[Development of a set of diagnostic test systems for analysis of expression of C-myc, mad1, max, p53, and E2F1 gene oncomarkers by the reverse transcription reaction-polymerase chain reaction alignment technique].

Based on the performed studies, the authors developed test systems to analyze the expression of mRNA of the p53, C-myc, mad1, max, and E2F1 genes. These test systems could reveal a statistically significant difference between follicular adenoma and carcinoma of the thyroid in their expression of p53 mRNA. It should be noted that the use of our developed test systems is promising when searching for the diagnostic and prognostic markers of cancer, analyzing, and creating the genetic networks characterizing this or that cancer.

[Hygienic aspects of human sensitization under the influence of biological factors of the dwelling environment].

The paper deals with the risk of occurrence and development of allergic diseases under the influence of biological housing factors, such as molds, staphylococci, ticks, and insects. It shows that biological domestic allergens are of high importance in human sensitization. There is evidence that supports the allergic hazard of the above factors to children and adults.

[Effect of excess D,L-tryptophan on the level of aminoacylation of tRNA by 14C-tryptophan]. / Vplyv nadlyshku D,L-triptofanu na riven' aminoatsyliuvannia tRNK 14C-triptofanom

The effect of the D,L-tryptophan excess in the rat organism was studied as applied to the level of amino acylation of tRNA by 14C-tryptophan. It is established that the introduction of tryptophan for three days in doses of 50 and 75 mg per 100 g of the living weight evokes a 24 and 40% decrease, respectively, in the level of amino acylation of the rat livel tRNA by 14C-tryptophan. The ability of accepting 14C-tryptophan in total preparations of the experimental rats liver tRNA considerably lower as compared to norm. A short-term heating in the presence of magnesium ions partially restores their decreased acceptor ability. This gives ground to suppose that the molecules of tRNAtr in the total preparation of the rat liver tRNA with a great tryptophan excess in the organism partially change their conformation and this is one of the reasons of the decrease in the level of tRNA amino acylation by 14C-tryptophan. The decrease in the protein synthesis at the first stage with the presence of a great tryptophan excess in the organism is evident to be also connected with the inhibition of tryptophanyl-tRNA-synthetase activity.