NATURALLY OCCURRING RADIONUCLIDE 40K BODY CONTENT OF KAKRAPAR GUJARAT SITE POPULATION.

An attempt was made to investigate the naturally occurring radionuclide 40K body content of the Kakrapar Gujarat Site (India) population by using whole-body counting method. The population comprises occupational workers (regular and contract personnel working at Kakrapar Atomic Power station) and students from various educational institutions. A total of 1056 subjects of different age groups and gender were monitored for 40K body content by using a Standfast-II Whole Body Counting System. The reactor released radionuclides such as 137Cs, 60Co and 131I are not detected in occupational workers of Kakrapar Atomic Power station which indicates that occupational workers are free from radioactive contamination. Based on the activity measurement, an effective dose due to 40K was estimated for different groups of the site population based on the dietary habits, sex and age. Statistical significant correlation was not observed in any of the groups. The annual effective dose due to 40K was found to be in the range of 52-243 µSv y-1 with an average of 133 ± 35 µSv y-1 for the vegetarian group (588 subjects), 60-223 µSv y-1 with an average of 136 ± 35 µSv y-1 for the non-vegetarian group (468 subjects), 52-243 µSv y-1 with an average of 134 ± 35 µSv y-1 for the male group (1007 subjects) and 67-203 µSv y-1 with an average of 128 ± 35 µSv y-1 for the female group (49 subjects), respectively. Among the four age groups of the population studied, an effective dose due to 40K is slightly higher in the age group of 20-29 (161 subjects). The annual effective dose due to 40K reported by UNSCEAR, 1982(3) is 180 µSv y-1.

Assessment of daily intake of trace elements by Kakrapar adult population through ingestion pathway.

Concentration of trace elements such as Fe, Cu, Ni, and Zn were measured in cereals, pulses, vegetables, fish, meat, milk, egg, and water samples collected around Kakrapar, Gujarat, India. A wide variation of the trace element concentration was observed among all the dietary matrices. The concentration of Fe is comparatively more in all the dietary matrices. The concentrations of these elements are translated into intake rates through ingestion pathways. Daily intake (milligrams/day) of Fe, Cu, Ni, and Zn by adult population of Kakrapar, Gujarat were 16.5 +/- 6.2, 3.3 +/- 1.2, 1.8 +/- 1.0, and 3.6 +/- 1.3, respectively. Dietary sources of Fe, Cu, and Ni by Kakrapar adult population are comparable with RDA. In case of Zn, the daily dietary intake is comparatively lower than that of RDA.

Evidence for the incorporation of [32P]orthophosphate into leaf inositol phospholipids.

Leaf discs of brinjal, tomato, sugar cane and maize rapidly incorporated [32P]orthophosphate into total phospholipids. Analyses of the labelled lipid extracts by thin-layer chromatography, autoradiography and comparison with inositol phospholipid standards demonstrated the labelling of phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate in addition to other phospholipids. The presence of polyphosphoinositides was further confirmed by deacylation of phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate and separation of the water-soluble products, glycerophosphoinositol phosphate and glycerophosphoinositol bisphosphate by formate exchange chromatography. Incorporation of [32P]orthophosphate into inositol phospholipids was time-dependent, with monoester phosphate groups attaining isotopic equilibrium within 90 min of incubation. After 2 h, incorporation of label into phosphatidylinositol, phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate was about 15, 10 and 3%, respectively, of the total phospholipids. The ratio of radioactivity in phosphatidylinositol/phosphatidylinositol monophosphate/phosphatidylinositol bisphosphate was about 5:5:1 in brinjal leaves. However, this ratio may be an overestimate of the amounts of inositol phospholipids present, as other lysophospholipids may comigrate with standards.