RESUMEN
Cytokinin (CK) is a key plant hormone, but one whose effects are often misunderstood, partly due to reliance on older data from before the molecular genetic age of plant science. In this mini-review, we examine the role of CK in controlling the reproductive shoot architecture of flowering plants. We begin with a long overdue re-examination of the role of CK in shoot branching, and discuss the relatively paucity of genetic evidence that CK does play a major role in this process. We then examine the role of CK in determining the number of inflorescences, flowers, fruit and seed that plants initiate during reproductive development, and how these are arranged in space and time. The genetic evidence for a major role of CK in controlling these processes is much clearer, and CK has profound effects in boosting the size and number of most reproductive structures. Conversely, the attenuation of CK levels during the reproductive phase likely contributes to reduced organ size seen later in flowering, and the ultimate arrest of inflorescence meristems during end-of-flowering. We finish by discussing how this information can potentially be used to improve crop yields.
Asunto(s)
Citocininas , Brotes de la Planta , Citocininas/metabolismo , Brotes de la Planta/crecimiento & desarrollo , Brotes de la Planta/metabolismo , Flores/crecimiento & desarrollo , Reproducción/fisiología , Regulación de la Expresión Génica de las Plantas , Meristema/crecimiento & desarrollo , Meristema/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismoRESUMEN
Many plants show strong heteroblastic changes in the shape and size of organs as they transition from juvenile to reproductive age. Most attention has been focused on heteroblastic development in leaves, but we wanted to understand heteroblastic changes in reproductive organ size. We therefore studied the progression of reproductive development in the model plant Arabidopsis thaliana, and found strong reductions in the size of flowers, fruit, seed, and internodes during development. These did not arise from correlative inhibition by older fruits, or from changes in inflorescence meristem size, but seemed to stem from changes in the size of floral organ primordia themselves. We hypothesized that environmental conditions might influence this heteroblastic pattern and found that the ambient temperature during organ initiation strongly influences organ size. We show that this temperature-dependent heteroblasty is dependent on FLOWERING LOCUS T (FT)-mediated signal integration, adding to the repertoire of developmental processes regulated by this pathway. Our results demonstrate that rising global temperatures will not affect just fertility, as is widely described, but also the size and seed number of fruits produced. However, we also show that such effects are not hard-wired, and that selective breeding for FT expression during reproductive development could mitigate such effects.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Inflorescencia , Transducción de Señal , Arabidopsis/crecimiento & desarrollo , Arabidopsis/genética , Arabidopsis/metabolismo , Inflorescencia/crecimiento & desarrollo , Inflorescencia/genética , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Flores/crecimiento & desarrollo , Flores/genética , Regulación de la Expresión Génica de las Plantas , TemperaturaRESUMEN
Plants integrate environmental information into their developmental program throughout their lifetime. Light and temperature are particularly critical cues for plants to correctly time developmental transitions. Here, we investigated the role of photo-thermal cues in the regulation of the end-of-flowering developmental transition in the model plant Arabidopsis (Arabidopsis thaliana). We found that increased day length and higher temperature during flowering promote earlier inflorescence arrest by accelerating the rate at which the inflorescence meristem (IM) initiates floral primordia. Specifically, we show that plants arrest at a photo-thermal threshold and demonstrate that this photo-thermally mediated arrest is mediated by the floral integrator FLOWERING LOCUS T (FT), a known activator of flowering. FT expression increased over the duration of flowering, peaking during IM arrest, and we show that this is necessary and sufficient for photo-thermally induced arrest. Our data demonstrate the role of light and temperature, through FT, as key regulators of end-of-flowering. Overall, our results have important implications for understanding and modulating the flowering duration of crop species in changing light and temperature conditions in a warming global climate.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Meristema , Arabidopsis/fisiología , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/fisiología , Flores/fisiología , Regulación de la Expresión Génica de las Plantas , Inflorescencia/fisiología , Meristema/fisiologíaRESUMEN
The fruit and seed produced by a small number of crop plants provide the majority of food eaten across the world. Given the growing global population, there is a pressing need to increase yields of these crops without using more land or more chemical inputs. Many of these crops display prominent 'fruit-flowering feedbacks', in which fruit produced early in sexual reproductive development can inhibit the production of further fruit by a range of mechanisms. Understanding and overcoming these feedbacks thus presents a plausible route to increasing crop yields 'for free'. In this review, we define three key types of fruit-flowering feedback, and examine how frequent they are and their effects on reproduction in a wide range of both wild and cultivated species. We then assess how these phenomenologically distinct phenomena might arise from conserved phytohormonal signalling events, particularly the export of auxin from growing organs. Finally, we offer some thoughts on the evolutionary basis for these self-limiting sexual reproductive patterns, and whether they are also present in the cereal crops that fundamentally underpin global diets.
Asunto(s)
Frutas , Reproducción , Retroalimentación , Semillas , Productos AgrícolasRESUMEN
To maximize reproductive success, flowering plants must correctly time entry and exit from the reproductive phase. While much is known about mechanisms that regulate initiation of flowering, end-of-flowering remains largely uncharacterized. End-of-flowering in Arabidopsis (Arabidopsis thaliana) consists of quasi-synchronous arrest of inflorescences, but it is unclear how arrest is correctly timed with respect to environmental stimuli and reproductive success. Here, we showed that Arabidopsis inflorescence arrest is a complex developmental phenomenon, which includes the arrest of the inflorescence meristem (IM), coupled with a separable "floral arrest" of all unopened floral primordia; these events occur well before visible inflorescence arrest. We showed that global inflorescence removal delays both IM and floral arrest, but that local fruit removal only delays floral arrest, emphasizing their separability. We tested whether cytokinin regulates inflorescence arrest, and found that cytokinin signaling dynamics mirror IM activity, while cytokinin treatment can delay both IM and floral arrest. We further showed that gain-of-function cytokinin receptor mutants can delay IM and floral arrest; conversely, loss-of-function mutants prevented the extension of flowering in response to inflorescence removal. Collectively, our data suggest that the dilution of cytokinin among an increasing number of sink organs leads to end-of-flowering in Arabidopsis by triggering IM and floral arrest.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Arabidopsis/metabolismo , Inflorescencia/genética , Inflorescencia/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Citocininas , Meristema/genética , Meristema/metabolismo , Regulación de la Expresión Génica de las Plantas , Flores/genética , Flores/metabolismoRESUMEN
The production of seed in flowering plants is complicated by the need to first invest in reproductive shoots, inflorescences, flowers, and fruit. Furthermore, in many species, it will be months between plants generating flowers and setting seed. How can plants therefore produce an optimal seed-set relative to environmental resources when the "reproductive architecture" that supports seed-set needs to be elaborated so far in advance? Here, we address this question by investigating the spatio-temporal control of reproductive architecture in Arabidopsis (Arabidopsis thaliana) and Brassica napus. We show that resource and resource-related signals such as substrate volume play a key role in determining the scale of reproductive effort, and that this is reflected in the earliest events in reproductive development, which broadly predict the subsequent reproductive effort. We show that a series of negative feedbacks both within and between developmental stages prevent plants from over-committing to early stages of development. These feedbacks create a highly plastic, homeostatic system in which additional organs can be produced in the case of reproductive failure elsewhere in the system. We propose that these feedbacks represent an "integrated dominance" mechanism that allows resource use to be correctly sequenced between developmental stages to optimize seed set.
Asunto(s)
Arabidopsis/fisiología , Brassica napus/fisiología , Flores/crecimiento & desarrollo , Arabidopsis/crecimiento & desarrollo , Brassica napus/crecimiento & desarrollo , Inflorescencia/crecimiento & desarrollo , ReproducciónRESUMEN
Plants must carefully coordinate their growth and development with respect to prevailing environmental conditions. To do this, plants can use a range of nutritional and non-nutritional information that allows them to proactively modulate their growth to avoid resource limitations. As is well-known to gardeners and horticulturists alike, substrate volume strongly influences plant growth, and maybe a key source of non-nutritional information for plants. However, the mechanisms by which these substrate volume effects occur remain unclear. Here, we show that wheat plants proactively modulate their shoot growth with respect to substrate volume, independent of nutrient availability. We show that these effects occur in two phases; in the first phase, the dilution of a mobile 'substrate volume-sensing signal' (SVS) allows plants to match their shoot (but not root) growth to the total size of the substrate, irrespective of how much of this they can occupy with their roots. In the second phase, the dilution of a less mobile 'root density-sensing signal' (RDS) allows plants to match root growth to actual rooting volume, with corresponding effects on shoot growth. We show that the effects of soil volume and plant density are largely interchangeable and that plants may use both SVS and RDS to detect their neighbours and to integrate growth responses to both volume and the presence of neighbours. Our work demonstrates the remarkable ability of plants to make proactive decisions about their growth, and has implications for mitigating the effects of dense sowing of crops in agricultural practice.
Asunto(s)
Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Triticum/crecimiento & desarrollo , Nutrientes/metabolismo , Raíces de Plantas/fisiología , Brotes de la Planta/fisiología , Suelo , Triticum/fisiologíaRESUMEN
The reproduction of flowering plants is an incredibly important process, both ecologically and economically. A huge body of work has examined the mechanisms by which flowering plants correctly time their entry into the reproductive phase (the 'floral transition'). However, the corresponding mechanisms by which plants exit the reproductive phase remain relatively neglected. In this review, we identify four developmental processes that contribute to the end-of-flowering; floral arrest, inflorescence meristem arrest, inflorescence activation and 'vegetative transition'. We highlight that, due to the highly divergent nature of reproductive systems among flowering plants, these processes are differently important for end-of-flowering in different species. For each of these processes, we examine recent advances in understanding the regulatory mechanisms that govern the process, and how these mechanisms determine the timing of end-of-flowering.
Asunto(s)
Flores , Magnoliopsida , Flores/genética , Regulación de la Expresión Génica de las Plantas , Inflorescencia , Meristema , ReproducciónRESUMEN
A well-defined set of regulatory pathways control entry into the reproductive phase in flowering plants, but little is known about the mechanistic control of the end-of-flowering despite this being a critical process for optimization of fruit and seed production. Complete fruit removal, or lack of fertile fruit-set, prevents timely inflorescence arrest in Arabidopsis, leading to a previous proposal that a cumulative fruit/seed-derived signal causes simultaneous 'global proliferative arrest'. Recent studies have suggested that inflorescence arrest involves gene expression changes in the inflorescence meristem that are, at least in part, controlled by the FRUITFULL-APETALA2 pathway; however, there is limited understanding of how this process is coordinated at the whole-plant level. Here, we provide a framework for the communication previously inferred in the global proliferative arrest model. We show that the end-of-flowering in Arabidopsis is not 'global' and does not occur synchronously between branches, but rather that the arrest of each inflorescence is a local process, driven by auxin export from fruit proximal to the inflorescence apex. Furthermore, we show that inflorescences are competent for arrest only once they reach a certain developmental age. Understanding the regulation of inflorescence arrest will be of major importance to extending and maximizing crop yields.
Asunto(s)
Arabidopsis/metabolismo , Frutas/metabolismo , Ácidos Indolacéticos/metabolismo , Inflorescencia/crecimiento & desarrollo , Arabidopsis/crecimiento & desarrollo , Transporte Biológico , Inflorescencia/metabolismoRESUMEN
BACKGROUND: Strigolactones (SLs) are an important class of carotenoid-derived signalling molecule in plants, which function both as exogenous signals in the rhizosphere and as endogenous plant hormones. In flowering plants, SLs are synthesized by a core pathway of four enzymes and are perceived by the DWARF14 (D14) receptor, leading to degradation of SMAX1-LIKE7 (SMXL7) target proteins in a manner dependent on the SCFMAX2 ubiquitin ligase. The evolutionary history of SLs is poorly understood, and it is not clear whether SL synthesis and signalling are present in all land plant lineages, nor when these traits evolved. RESULTS: We have utilized recently-generated genomic and transcriptomic sequences from across the land plant clade to resolve the origin of each known component of SL synthesis and signalling. We show that all enzymes in the core SL synthesis pathway originated at or before the base of land plants, consistent with the previously observed distribution of SLs themselves in land plant lineages. We also show that the late-acting enzyme LATERAL BRANCHING OXIDOREDUCTASE (LBO) may be considerably more ancient than previously thought. We perform a detailed phylogenetic analysis of SMXL proteins and show that specific SL target proteins only arose in flowering plants. We also assess diversity and protein structure in the SMXL family, identifying several previously unknown clades. CONCLUSIONS: Overall, our results suggest that SL synthesis is much more ancient than canonical SL signalling, consistent with the idea that SLs first evolved as rhizosphere signals and were only recruited much later as hormonal signals.
Asunto(s)
Embryophyta , Lactonas/metabolismo , Magnoliopsida , Reguladores del Crecimiento de las Plantas , Proteínas de Plantas/genética , Embryophyta/clasificación , Embryophyta/genética , Embryophyta/metabolismo , Evolución Molecular , Magnoliopsida/clasificación , Magnoliopsida/genética , Magnoliopsida/metabolismo , Filogenia , Reguladores del Crecimiento de las Plantas/biosíntesis , Reguladores del Crecimiento de las Plantas/metabolismoRESUMEN
The spatio-temporal production of flowers is key to determining reproductive fitness in most flowering plants and yield in many crop species, but the mechanisms regulating this 'reproductive architecture' are poorly characterized. Here, we show that in members of the Brassicaceae, total flower number is largely independent of inflorescence number and that the proportion of flowers initiated on the secondary inflorescences represents ~50% of total floral production, irrespective of secondary inflorescence number. This '50% rule' acts as a coordinating principle for reproductive development in Brassicaceae, and similar principles may operate in other species. Our findings suggest that inflorescences continue to compete with each other for a fixed pool of meristematic potential after their activation.
Asunto(s)
Brassicaceae/crecimiento & desarrollo , Flores/crecimiento & desarrollo , Inflorescencia/crecimiento & desarrollo , ReproducciónRESUMEN
Hypocotyl phototropism of etiolated Arabidopsis seedlings is primarily mediated by the blue-light receptor kinase phototropin 1 (phot1). Phot1-mediated curvature to continuous unilateral blue light irradiation (0.5 µmol m(-2) s(-1)) is enhanced by overhead pre-treatment with red light (20 µmol m(-2) s(-1) for 15 min) through the action of phytochrome (phyA). Here, we show that pre-treatment with blue light is equally as effective in eliciting phototropic enhancement and is dependent on phyA. Although blue light pre-treatment was sufficient to activate early phot1 signaling events, phot1 autophosphorylation in vivo was not found to be saturated, as assessed by subsequently measuring phot1 kinase activity in vitro. However, enhancement effects by red and blue light pre-treatment were not observed at higher intensities of phototropic stimulation (10 µmol m(-2) s(-1)). Phototropic enhancement by red and blue light pre-treatments to 0.5 µmol m(-2) s(-1) unilateral blue light irradiation was also lacking in transgenic Arabidopsis where PHOT1 expression was restricted to the epidermis. Together, these findings indicate that phyA-mediated effects on phot1 signaling are restricted to low intensities of phototropic stimulation and originate from tissues other than the epidermis.
RESUMEN
Floral morphology determines the pattern of pollen transfer within and between individuals. In hermaphroditic species, the spatial arrangement of sexual organs influences the rate of self-pollination as well as the placement of pollen in different areas of the pollinator's body. Studying the evolutionary modification of floral morphology in closely related species offers an opportunity to investigate the causes and consequences of floral variation. Here, we investigate the recurrent modification of flower morphology in three closely related pairs of taxa in Solanum section Androceras (Solanaceae), a group characterized by the presence of two morphologically distinct types of anthers in the same flower (heteranthery). We use morphometric analyses of plants grown in a common garden to characterize and compare the changes in floral morphology observed in parallel evolutionary transitions from relatively larger to smaller flowers. Our results indicate that the transition to smaller flowers is associated with a reduction in the spatial separation of anthers and stigma, changes in the allometric relationships among floral traits, shifts in pollen allocation to the two anther morphs and reduced pollen : ovule ratios. We suggest that floral modification in this group reflects parallel evolution towards increased self-fertilization and discuss potential selective scenarios that may favour this recurrent shift in floral morphology and function.