RESUMEN
Advances in molecular genetics have identified several species of RNA that fail to translate - hence the non-coding RNAs. The two major groups within this class of nucleic acids are microRNAs (miRNA) and long non-coding RNAs (lncRNA). There is growing body of evidence supporting the view that these molecules have regulatory effect on both DNA and RNA. The objective of this brief review is to explain the molecular genetic of these molecules, to summarize their potential as mediators of disease, and to highlight their value as diagnostic markers and as tools in disease management.
Asunto(s)
Artritis Reumatoide/genética , Enfermedades Cardiovasculares/genética , Diabetes Mellitus Tipo 2/genética , Genoma Humano , Neoplasias/genética , ARN Largo no Codificante/genética , Animales , Artritis Reumatoide/diagnóstico , Artritis Reumatoide/metabolismo , Artritis Reumatoide/patología , Biomarcadores/metabolismo , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/patología , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patología , Hematopoyesis/genética , Humanos , Personal de Laboratorio/educación , MicroARNs/genética , MicroARNs/metabolismo , Neoplasias/diagnóstico , Neoplasias/metabolismo , Neoplasias/patología , ARN Largo no Codificante/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Índice de Severidad de la EnfermedadRESUMEN
In order to promote understanding of UK governance and assurance relating to electronic health records research, we present and discuss the role of the Independent Scientific Advisory Committee (ISAC) for MHRA database research in evaluating protocols proposing the use of the Clinical Practice Research Datalink. We describe the development of the Committee's activities between 2006 and 2015, alongside growth in data linkage and wider national electronic health records programmes, including the application and assessment processes, and our approach to undertaking this work. Our model can provide independence, challenge and support to data providers such as the Clinical Practice Research Datalink database which has been used for well over 1,000 medical research projects. ISAC's role in scientific oversight ensures feasible and scientifically acceptable plans are in place, while having both lay and professional membership addresses governance issues in order to protect the integrity of the database and ensure that public confidence is maintained.
Asunto(s)
Acceso a la Información/ética , Comités Consultivos , Investigación Biomédica , Registros Electrónicos de Salud , Comités Consultivos/organización & administración , Bases de Datos Factuales , Agencias Gubernamentales , Humanos , Medición de Riesgo , Reino UnidoRESUMEN
A study was conducted to examine the duration of anthelmintic effect of copper oxide wire particles (COWP) in grazing goats, as data for the persistence of efficacy of COWP in this host species is limited. Forty-eight indigenous male goats were infected naturally by grazing them on Haemonchus contortus-infected pasture. When the faecal egg count (FEC) in the goats was 3179 ± 540 eggs per gram of faeces (mean ± standard error), half the animals were treated with 4 g COWP (day 0; mean live weight=25.5 ± 0.8 kg). Eight treated (COWP) and eight non-treated (CONTROL) goats were removed from the pasture on each of days 7, 28 and 56, maintained for 27 or 29 days in concrete pens and then humanely slaughtered for nematode recovery. Mean liver copper levels were in the high range in the goats removed from pasture at day 7 (treated: 191 ± 19.7 ppm; untreated: 120 ± 19.7 ppm; P=0.022), but had dropped to normal levels at days 28 and 56. The mean H. contortus burdens of the treated versus the non-treated goats were, respectively, 184 ± 48 and 645 ± 152 for the goats removed from pasture at day 7 (71% reduction; P=0.004), 207 ± 42 and 331 ± 156 at day 28 (37% reduction; P=0.945) and 336 ± 89 and 225 ± 53 at day 56 (-49% reduction; P=0.665). Weekly monitoring of FECs after treatment until slaughter indicated that the COWP-treated goats had lower FECs than the controls, the treatment main effect being significant at days 7, 28 and 56 (P<0.01). The day main effect and the treatment × day interaction were only significant for the goats removed from pasture at day 28 (P ≤ 0.001). Packed cell volumes increased during the course of the experiment (day, P<0.001), but the treatment main effect was significant only for the goats removed from pasture at day 28 (CONTROL 28 d, 28.65 ± 0.52%Asunto(s)
Antihelmínticos/administración & dosificación
, Cobre/administración & dosificación
, Enfermedades de las Cabras/tratamiento farmacológico
, Hemoncosis/veterinaria
, Haemonchus/efectos de los fármacos
, Animales
, Antihelmínticos/análisis
, Peso Corporal
, Cobre/análisis
, Cynodon/parasitología
, Heces/parasitología
, Enfermedades de las Cabras/parasitología
, Cabras
, Hemoncosis/tratamiento farmacológico
, Hemoncosis/parasitología
, Hematócrito/veterinaria
, Herbivoria
, Larva
, Hígado/química
, Masculino
, Recuento de Huevos de Parásitos/veterinaria
, Resultado del Tratamiento
RESUMEN
Haemonchosis is considered to be the most economically important gastrointestinal disease of small ruminants in the tropics and subtropics. However, chemical anthelmintics, which were the mainstay of control, have been compromised by a high prevalence of resistance worldwide. Copper oxide wire particles (COWP) have been shown to have anthelmintic effects, but few studies have examined their use under field conditions. The use of COWP was therefore evaluated as a tactical anthelmintic treatment in indigenous goats raised under communal farming conditions in Bergville, KwaZulu-Natal Province, South Africa. At the beginning of the summer rainfall season (October 2007), the faecal egg counts of 172 female goats belonging to 15 farmers were determined and this sampling continued every four weeks until the second week of January 2008. The goats within each of the 15 herds were ranked according to their faecal egg counts for this week. The goats were sequentially paired off within each ranking starting with those goats with the highest counts. One goat from each pair was randomly allocated to a treated or control group. Two weeks later, a 4 g COWP bolus was randomly administered to each goat in the treated group. Faecal egg counts were carried out on the goats two weeks following treatment, and the sampling of the goats then proceeded every four weeks until October 2008. Except for the six-week period prior to the administration of the COWP, the goats were examined according to the FAMACHA(©) system and symptomatically treated with 12 mg/kg levamisole when anaemic. The percentage reduction in faecal egg count due to the COWP treatment was 89.0%. Mean pre- and post-treatment faecal egg counts for the COWP-treated group (n=73) were 2347 eggs per gram of faeces (epg) and 264 epg, respectively. The corresponding values for the untreated controls (n=66) were 2652 epg and 2709 epg. The prevalence of Haemonchus spp. larvae in pre- and post-treatment faecal cultures was 72% and 46%, respectively. Symptomatic anthelmintic treatments in combination with mid-summer tactical treatments with COWP appear to be useful strategies for the control of Haemonchus contortus in indigenous goats in this farming system and this approach could have application in other similar agro-ecological zones.
Asunto(s)
Antihelmínticos/uso terapéutico , Cobre/uso terapéutico , Enfermedades de las Cabras/tratamiento farmacológico , Hemoncosis/veterinaria , Levamisol/uso terapéutico , Análisis de Varianza , Animales , Constitución Corporal , Peso Corporal , Heces/parasitología , Femenino , Cabras , Hemoncosis/tratamiento farmacológico , Haemonchus/fisiología , Hematócrito , Recuento de Huevos de Parásitos/veterinaria , Lluvia , Sudáfrica , Temperatura , Resultado del TratamientoRESUMEN
A 3-year grazing trial was performed during 2003-2005 on a commercial steer-producing farm in Sweden to study performance of second-season grazing (SSG) cattle following different levels of parasite exposure during their first grazing season. Initially, groups of 10 first-season grazing (FSG) cattle were each year assigned to four parasite control strategies: (1) turn-out onto pasture that during the previous year was grazed by SSG cattle, followed by a mid-July move to aftermath, (2) supplementation with concentrate and roughage for 4 weeks after turn-out, (3) no treatment, or (4) anthelmintic treatment (injectable doramectin) every fourth week. All animals were set stocked, except for those in group one. Next spring and following housing the cattle were turned out for their second grazing season onto approximately 25 ha communal pasturelands as a common mob. Weighing, faecal sampling and blood collection were performed at turn-out and then every 4 weeks for the 20-week grazing season. Faecal samples were also collected on day 10 after turn-out for detection of coccidian oocysts. Antibodies to Dictyocaulus viviparus were analysed at the time of their second housing period, and when elevated levels were recorded, stored serum samples from seropositive animals were analysed retrospectively. Results showed early-season weight losses of up to 47 kg in the SSG cattle. However, faecal egg counts were generally low and there was no correlation between SSG performance and treatment history as FSG cattle. Still, cumulative egg counts were significantly higher in animals that had been treated with anthelmintic as FSG cattle but serum pepsinogen concentrations showed no significant differences and the output of Eimeria alabamensis oocysts seldom exceeded 10,000 oocysts per gram faeces. Antibodies to D. viviparus were observed from July 2004 and from June 2005 but not in 2003. In 2004 and 2005, 64% and 83% of the animals, respectively, were seropositive for D. viviparus. It is concluded that weight gain penalties resulting from different levels of parasite infections the first grazing season remained during the second grazing season in 2004 and 2005 but no differences in weight gain could be identified in SSG cattle that had experienced different parasite control measures during their first grazing season. This was under conditions where the level of pasture infectivity was low to moderate during the second grazing period. However, D. viviparus was demonstrated in SSG animals during the two last years of the study and was an important confounding factor.
Asunto(s)
Crianza de Animales Domésticos/métodos , Enfermedades de los Bovinos/prevención & control , Enfermedades Parasitarias en Animales/prevención & control , Animales , Anticuerpos Antihelmínticos/sangre , Bovinos , Enfermedades de los Bovinos/sangre , Enfermedades de los Bovinos/inmunología , Heces/parasitología , Masculino , Enfermedades Parasitarias en Animales/tratamiento farmacológico , Factores de Tiempo , Tiempo (Meteorología) , Aumento de PesoRESUMEN
The high prevalence of resistance of Haemonchus contortus to all major anthelmintic groups has prompted investigations into alternative control methods in South Africa, including the use of copper oxide wire particle (COWP) boluses. To assess the efficacy of COWP against H. contortus in indigenous South African goats, 18 male faecal egg-count-negative goats were each given ca.1200 infective larvae of H. contortus three times per week during weeks 1 and 2 of the experiment. These animals made up an "established" infection group (ESTGRP). At the start of week 7, six goats were each given a 2-g COWP bolus orally; six goats received a 4-g COWP bolus each and six animals were not treated. A further 20 goats constituted a "developing" infection group (DEVGRP). At the beginning of week 1, seven of the DEVGRP goats were given a 2-g COWP bolus each; seven goats were treated with a 4-g COWP bolus each and no bolus was given to a further six animals. During weeks 1-6, each of these DEVGRP goats was given ca. 400 H. contortus larvae three times per week. All 38 goats were euthanized for worm recovery from the abomasa and small intestines in week 11. In the ESTGRP, the 2-g and 4-g COWP boluses reduced the worm burdens by 95% and 93%, respectively compared to controls (mean burden+/-standard deviation, SD: 23+/-33, 30+/-56 and 442+/-518 worms, P=0.02). However, in the DEVGRP goats, both the 2-g and 4-g COWP treatments were ineffective in reducing the worm burdens relative to the controls (mean burdens+/-SD: 1102+/-841, 649+/-855, 1051+/-661 worms, P=0.16). Mean liver copper levels did not differ between the ESTGRP goats treated with 2-g COWP, 4-g COWP or no COWP (mean+/-standard error of the mean, SEM, in ppm: 93.7+/-8.3; 101.5+/-8.3; 71.8+/-8.3, P=0.07) nor did they differ between the DEVGRP goats (mean+/-SEM, in ppm: 74.1+/-9.1; 75.4+/-9.1; 74.9+/-10.0, P>0.99). The copper values were considered adequate, but not high, for goats. The COWP boluses have the potential to be used in the place of conventional anthelmintics for the control of established H. contortus infections in indigenous South African goats, but their use as part of an integrated approach to control H. contortus in the field must be fully investigated.
Asunto(s)
Antinematodos/uso terapéutico , Cobre/uso terapéutico , Enfermedades Gastrointestinales/veterinaria , Enfermedades de las Cabras/tratamiento farmacológico , Hemoncosis/veterinaria , Animales , Antinematodos/administración & dosificación , Cobre/administración & dosificación , Heces/parasitología , Enfermedades Gastrointestinales/tratamiento farmacológico , Enfermedades Gastrointestinales/parasitología , Enfermedades de las Cabras/epidemiología , Cabras , Hemoncosis/tratamiento farmacológico , Hemoncosis/epidemiología , Haemonchus , Masculino , Recuento de Huevos de Parásitos , Sudáfrica/epidemiologíaRESUMEN
The benefit of long-term feeding of fresh or ensiled cassava foliage on gastrointestinal parasite in goats was evaluated. Eighteen male goats (15.15 +/- 2.83 kg and between 4-6 months) were randomly allocated into three treatments supplemented with 200 g of wheat bran head(-1) day(-1). All groups were fed ad-libitum on either grass (CO), fresh cassava (CaF) or ensiled cassava foliage (CaS). At the beginning of the trial, each goat was inoculated with 3000 L3 containing approximately 50% Haemonchus contortus. Individual LWt, FEC and PCV were measured at weekly intervals for 10 weeks. At the termination of the experiment all goats were slaughtered for worm recovery and enumeration. The goats in CaF and CaS had similar weight gains while those in CO lost weight (p < 0.05) through the trial. FEC in CaF and CaS were lower (p < 0.05) than CO during the patency of parasite infections, but there was no difference between CaF and CaS goats. PCV of all groups decreased from above 30% to around 25% at the end of the trial. The compositions of established worm burdens were mainly H. contortus (19-40%) and Trichostrongylus colubriformis (55-76%). TWB did not differ among the groups, however, CaS significantly reduced H. contortus burdens, as compared to CaF and CO (p < or = 0.005). Thus, ensiled cassava foliage reduced the H. contortus population while the fresh foliage only reduced worm fecundity.
Asunto(s)
Enfermedades de las Cabras/tratamiento farmacológico , Hemoncosis/veterinaria , Parasitosis Intestinales/veterinaria , Manihot , Fitoterapia/veterinaria , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Heces , Enfermedades de las Cabras/sangre , Enfermedades de las Cabras/epidemiología , Cabras/crecimiento & desarrollo , Hemoncosis/sangre , Hemoncosis/tratamiento farmacológico , Hemoncosis/epidemiología , Haemonchus/crecimiento & desarrollo , Haemonchus/aislamiento & purificación , Hematócrito/veterinaria , Parasitosis Intestinales/sangre , Parasitosis Intestinales/tratamiento farmacológico , Parasitosis Intestinales/epidemiología , Masculino , Infecciones por Nematodos/sangre , Infecciones por Nematodos/tratamiento farmacológico , Infecciones por Nematodos/epidemiología , Infecciones por Nematodos/veterinaria , Recuento de Huevos de Parásitos/veterinaria , Distribución Aleatoria , Ensilaje , Clima Tropical , Aumento de PesoRESUMEN
A 3-year grazing trial (2002-2004) was conducted on a commercial beef cattle farm in south-central Sweden to assess different methods of parasite control. This paper focuses on the dynamics of the free-living larval stages, whereas data on performance and within-host parasitological variables are presented in a complementary paper. Each year in May, 4 groups of 10 first-season grazing (FSG) steers were turned out on to separate 2ha paddocks and subjected to the following strategies: (1) spring turn-out on to pasture which had been grazed the previous year by second-season grazing (SSG) steers (paddock RT), followed by a move to aftermath (paddock AM) after 10 weeks (mid-July), (2) supplementary feeding with concentrate and hay for 4 weeks following turn-out (paddock FD), set stocked, (3) untreated control (paddock UT), set stocked and (4) anthelmintic treated control (paddock DO), set stocked. All paddocks were assigned a new set of FSG cattle each year whereas the treatments remained the same. Pasture infectivity were monitored partly by two tracer calves that grazed each paddock along with the FSG calves for 3 weeks after turn-out and prior to housing, partly by analysis of herbage samples for infective larvae (L3) that were collected from each paddock at monthly intervals between April and October. The predominant genera found were Cooperia and Ostertagia. Tracers grazing paddock RT overall harboured less worms, and in particular less Ostertagia spp., and tracers grazing paddock AM in mid-July harboured insignificant numbers of nematodes compared to tracers on the FD and UT paddocks. Although total worm counts varied between groups, smaller numbers were generally observed early in the grazing-season (May), compared to close to housing (September) when inhibited early L4 larvae were almost exclusively found. Results observed from herbage samples showed high numbers of L3 in spring before the time of turn-out, compared to around housing. In conclusion, the rotation control strategy showed promising results and provided a turn-out pasture that was 'nematode safe' to FSG cattle the following spring, whereas the feeding strategy failed as applied in this experiment.
Asunto(s)
Alimentación Animal , Crianza de Animales Domésticos/métodos , Enfermedades de los Bovinos/prevención & control , Enfermedades de los Bovinos/parasitología , Helmintiasis Animal/prevención & control , Estaciones del Año , Animales , Bovinos , Dieta , Helmintiasis Animal/parasitología , Factores de TiempoRESUMEN
The objective of the study was to investigate different aspects on the efficacy of three anthelmintics on cyathostomin nematodes of Swedish horses. A faecal egg count reduction (FECR) test was performed on 26 farms. Horses were treated orally with recommended doses of ivermectin, pyrantel pamoate or fenbendazole. Faecal samples were collected on the day of deworming and 7, 14 and 21 days later. No resistance was shown against ivermectin; the FECR was constantly >99%. The effect of pyrantel was assessed as equivocal in 6 farms 14 days after treatment; the mean FECR was 99%. As many as 72% of the fenbendazole-treated groups met the criteria for resistance; the mean FECR was 86%, ranging from 56% to 100%. A re-investigation of two farms where pyrantel resistance had been suspected clearly revealed unsatisfactory efficacy of pyrantel on one of these farms; the FECR varied from 72% to 89%. Twenty-six of the horses previously dosed with pyrantel or fenbendazole, and which still excreted >/=150 eggs per gram of faeces 14 days after treatment, were dewormed with ivermectin and fenbendazole or pyrantel in order to eliminate the remaining cyathostomins. A total of 13 cyathostomin species were identified from horses that initially received fenbendazole and seven species were identified from pyrantel-treated individuals. The egg reappearance period (ERP) following treatment with ivermectin and pyrantel was investigated on two farms. The shortest ERP after ivermectin treatment was 8 weeks and after pyrantel was 5 weeks. We conclude that no substantial reversion to benzimidazole susceptibility had taken place, although these drugs have scarcely been used (<5%) in horses for the last 10 years. Pyrantel-resistant populations of cyathostomins are present on Swedish horse farms, but the overall efficacy of pyrantel is still acceptable.
Asunto(s)
Antihelmínticos/uso terapéutico , Infecciones Equinas por Strongyloidea/tratamiento farmacológico , Strongylus/crecimiento & desarrollo , Animales , Resistencia a Medicamentos , Heces/parasitología , Fenbendazol/uso terapéutico , Caballos , Ivermectina/uso terapéutico , Recuento de Huevos de Parásitos/veterinaria , Prevalencia , Pamoato de Pirantel/uso terapéutico , Infecciones Equinas por Strongyloidea/epidemiología , Infecciones Equinas por Strongyloidea/parasitología , Suecia/epidemiologíaRESUMEN
This study was Conducted To Evaluate The Anthelmintic Effect Of Neem (azadirachta Indica) On Nematode Parasites Of Sheep. Twelve Santa Ines Cross Bred Sheep From A Government Farm were randomly selected and equally divided into control (n = 6) and treated groups (n =6). Faecal egg counts (FEC) using the modified McMaster technique and the FAMACHA score for assessing clinical anaemia were carried out daily and recorded for 6 weeks. At the end of the study all the animals were slaughtered and the total worm count (TWC) was done. The results of FEC showed that there was no significant difference between the control and treated group (p = 0.081). However, worm burden estimations showed that the number of parasites was significantly higher in the control group compared to the treated group (p < 0.05). This result indicated that feeding Neem had an effect on worm numbers in sheep, but was not reflected in their faecal egg counts. Further work is needed to reconfirm the effect of Neem on helminth infections of sheep.
Asunto(s)
Azadirachta , Nematodos/efectos de los fármacos , Hojas de la Planta , Ovinos/parasitología , Animales , Femenino , Masculino , Recuento de Huevos de ParásitosRESUMEN
The anthelmintic resistance status was investigated of nematode parasites of sheep and goats owned by smallholder farmers in communities that received breeding stock from a source where a high level of anthelmintic resistance has been reported. The investigation used the faecal egg count reduction technique, whereby suitable animals within each of eight separate communities were pooled to achieve the numbers required to conduct separate tests for both sheep and goats. Anthelmintics tested were albendazole (ABZ), tetramisole (TET), a combination (ABZ + TET) and ivermectin (IVM), at the manufacturers' recommended dose rates. Results showed that there was no evidence of anthelmintic resistance in nematode parasites of either sheep an goats in any community. This indicates that dilution of resistant parasites imported with introduced breeding stock, and the low selection pressure imposed by the smallholder farmers themselves, has prevented anthelmintic resistance from emerging in nematode parasites of small ruminants in these communities.
Asunto(s)
Antihelmínticos/farmacología , Resistencia a Medicamentos , Enfermedades de las Cabras/tratamiento farmacológico , Infecciones por Nematodos/veterinaria , Enfermedades de las Ovejas/tratamiento farmacológico , Animales , Relación Dosis-Respuesta a Droga , Quimioterapia Combinada , Etiopía/epidemiología , Heces/parasitología , Cabras , Nematodos/efectos de los fármacos , Infecciones por Nematodos/tratamiento farmacológico , Recuento de Huevos de Parásitos/veterinaria , Pruebas de Sensibilidad Parasitaria/veterinaria , OvinosRESUMEN
To evaluate non-chemical strategies to control pasture-borne parasites in first-season grazing (FSG) cattle, a 3-year grazing trial was conducted during 2002-2004 on naturally infected pastures on a commercial beef cattle farm in Sweden. A uniform pasture was divided in 4 equal 2 ha paddocks onto each of which 10, 5-9 months old dairy breed steer calves were allocated at turn-out in May each year. Two strategies were evaluated: (1) turn-out onto pasture which had been grazed the previous year by second-season grazing (SSG) steers, followed by a move to aftermath in mid-July (RT) and (2) supplementation with concentrate and roughage for 4 weeks from turn-out (FD). Comparisons were made with an untreated (UT), and an anthelmintic treated control group (DO). Animal parasitology and performance were monitored monthly throughout the 20 weeks grazing period. Additional sampling occasions were performed on day 9 (for coccidia) and 10 weeks after turn-out (mid-July). Due to clinical parasitic gastro-enteritis (PGE), salvage treatments were performed on all animals in group FD approximately 7 weeks after turn-out in 2003 and of three animals in group UT 5 weeks after turn-out in 2004. In 2003, the geometric mean oocyst excretion 9 days after turn-out was approximately 150,000 opg of mainly Eimeria alabamensis in group FD, and in 2004 approximately 180,000 opg in group UT. Apart from the DO group, geometric mean faecal egg counts (FEC) were between 80 and 400 epg 4 weeks after turn-out. Mean serum pepsinogen concentrations (SPC) of approximately 3.6 U tyrosine were recorded in the FD and UT groups from late August 2002. In 2003 and 2004, mean concentrations in these groups were between 4.1 and 7.2 U tyrosine 8 weeks after turn-out. By the end of the three grazing seasons the average weight gain difference compared to the DO group was for FD -29, -38 and -5 kg and for RT -4, -21 and +14 kg, and compared to the UT group -18, +2 and +22 for FD and +7, +19 and +41 kg for group RT. In conclusion, the rotation control strategy showed promising results, whereas the strategic feeding was poor from a parasite control standpoint.
Asunto(s)
Enfermedades de los Bovinos/prevención & control , Enfermedades de los Bovinos/parasitología , Coccidiosis/veterinaria , Industria Lechera/métodos , Infecciones por Nematodos/veterinaria , Animales , Antihelmínticos/administración & dosificación , Antihelmínticos/farmacología , Anticuerpos Antihelmínticos/sangre , Anticuerpos Antiprotozoarios/sangre , Bovinos , Coccidiosis/inmunología , Coccidiosis/prevención & control , Suplementos Dietéticos , Eimeria/aislamiento & purificación , Heces/parasitología , Ivermectina/administración & dosificación , Ivermectina/análogos & derivados , Ivermectina/farmacología , Masculino , Nematodos/aislamiento & purificación , Infecciones por Nematodos/inmunología , Infecciones por Nematodos/prevención & control , Recuento de Huevos de Parásitos/veterinaria , Pepsinógeno A/sangre , Distribución Aleatoria , Factores de Tiempo , Aumento de Peso/fisiologíaRESUMEN
Nematode parasite infections of semi-domestic reindeer grazing in their natural habitat in northern Finland were monitored for approximately 2 years. This was achieved by monthly faecal egg counts of male and female calves and adult females from an experimental reindeer herd, in addition to estimating the acquisition of nematode infection from pasture using tracer reindeer calves. The most abundant parasite was Ostertagia gruehneri in the worm counts of tracer animals and in faecal egg counts of adult female reindeer. Capillaria sp. eggs were detected in calves and adults, but Nematodirinae eggs were only recovered from calves. Faecal egg counts showed variations between months for each nematode species, with male and female calves shedding similar numbers of eggs. During each year, calves shed more Capillaria sp. eggs than adult female reindeer, but similar numbers of O. gruehneri eggs. Egg counts of O. gruehneri were more abundant in late summer-autumn (July-September), whereas Capillaria sp. and the Nematodirinae dominated the winter months (November-February). The seasonal trends of adult worm burdens of O. gruehneri in the tracers paralleled the egg count patterns. Capillaria sp. was not detected in tracer worm counts. Tracer worm burdens showed that the proportion of inhibited larvae of O. gruehneri and Nematodirinae steadily increased from spring to early winter, followed by a decline and a commensurate increase in the number of adult parasites in the second summer. This investigation showed that parasite transmission occurs continuously throughout the year for nematode parasites of reindeer in northern Finland.
Asunto(s)
Nematodos/crecimiento & desarrollo , Infecciones por Nematodos/veterinaria , Reno/parasitología , Estaciones del Año , Animales , Antihelmínticos/administración & dosificación , Regiones Árticas/epidemiología , Capillaria/crecimiento & desarrollo , Capillaria/aislamiento & purificación , Heces/parasitología , Femenino , Finlandia/epidemiología , Intestino Delgado/parasitología , Ivermectina/administración & dosificación , Masculino , Nematodos/aislamiento & purificación , Infecciones por Nematodos/epidemiología , Ostertagia/crecimiento & desarrollo , Ostertagia/aislamiento & purificación , Recuento de Huevos de Parásitos/veterinaria , Dinámica Poblacional , Nieve , Estrongílidos/crecimiento & desarrollo , Estrongílidos/aislamiento & purificaciónRESUMEN
Trials were conducted on 3 commercial sheep farms in Sweden to assess the effect of administering spores of the nematode trapping fungus, Duddingtonia flagrans, together with supplementary feed to lactating ewes for the first 6 weeks from turn-out on pastures in spring. Also control groups of ewes, receiving only feed supplement, were established on all 3 farms. Groups were monitored by intensive parasitological investigation. The ewes and their lambs were moved in late June to saved pastures for summer grazing, the lambs receiving an anthelmintic treatment at this time. After approximately 6 weeks on summer pasture the lambs were weaned, treated a second time with anthelmintic, and returned to their original lambing pastures for finishing. Decisions as to when lambs were to be marketed were entirely at the discretion of the farmer co-operators. No difference in lamb performance was found between the two treatments on all three farms. This was attributed to the high levels of nutrition initially of the ewes limiting their post-partum rise in nematode faecal egg counts in spring, which in turn resulted in low levels of nematode infection on pastures throughout the autumn period. Additionally, pastures were of good quality for the lambs during the finishing period, so they grew at optimal rates as far as the farmers were concerned.
Asunto(s)
Ascomicetos/fisiología , Nematodos/crecimiento & desarrollo , Infecciones por Nematodos/veterinaria , Control Biológico de Vectores/métodos , Enfermedades de las Ovejas/prevención & control , Crianza de Animales Domésticos/métodos , Animales , Animales Recién Nacidos/crecimiento & desarrollo , Heces/parasitología , Femenino , Lactancia , Masculino , Infecciones por Nematodos/prevención & control , Recuento de Huevos de Parásitos/veterinaria , Poaceae , Distribución Aleatoria , Estaciones del Año , Ovinos , SueciaRESUMEN
Despite the extraordinary success in the development of anthelmintics in the latter part of the last century, helminth parasites of domestic ruminants continue to pose the greatest infectious disease problem in grazing livestock systems worldwide. Newly emerged threats to continuing successful livestock production, particularly with small ruminants, are the failure of this chemotherapeutic arsenal due to the widespread development of anthelmintic resistance at a time when the likelihood of new products becoming commercially available seems more remote. Changing public attitudes with regards to animal welfare, food preferences and safety will also significantly impact on the ways in which livestock are managed and their parasites are controlled. Superimposed on this are changes in livestock demographics internationally, in response to evolving trade policies and demands for livestock products. In addition, is the apparently ever-diminishing numbers of veterinary parasitology researchers in both the public and private sectors. Industries, whether being the livestock industries, the public research industries, or the pharmaceutical industries that provide animal health products, must adapt to these changes. In the context of helminth control in ruminant livestock, the mind-set of 'suppression' needs to be replaced by 'management' of parasites to maintain long-term profitable livestock production. Existing effective chemical groups need to be carefully husbanded and non-chemotherapeutic methods of parasite control need to be further researched and adopted, if and when, they become commercially available. This will require veterinary parasitology researchers from both the public and private sectors to work in close co-operation to ensure 'sustainability' - not only of the livestock industries that they service - but also for their very own activities and enterprises.
Asunto(s)
Bienestar del Animal , Animales Domésticos , Antihelmínticos/uso terapéutico , Seguridad de Productos para el Consumidor , Helmintiasis Animal/prevención & control , Animales , Residuos de Medicamentos , Resistencia a Medicamentos , Humanos , Carne/normas , Control Biológico de Vectores , InvestigaciónRESUMEN
Twenty-four calves unexposed to pasture were allocated to four groups and inoculated with either two doses of 5 million Eimeria alabamensis oocysts at turn-out (E), 90,000 L3 of Ostertagia ostertagi and Cooperia oncophora divided on six occasions (N) or both oocysts and larvae as above (E + N). A control group was left uninoculated (C). For 10 weeks, the groups grazed in separate uniform paddocks not previously grazed by cattle. By day 5, most calves in groups E and E + N developed clinical coccidiosis that resulted in reduced weight gain compared to C and N. Mean trichostrongylid faecal egg counts in groups N and E + N never exceeded 300 eggs per gram of faeces, and average serum pepsinogen levels were less than 3.8 U tyrosine. This experiment demonstrates the potential impact of E. alabamensis on the performance of previously unexposed calves, whereas no aggravated effects were observed due to concurrent infections with gastrointestinal nematodes.
Asunto(s)
Enfermedades de los Bovinos/patología , Coccidiosis/veterinaria , Eimeria , Trichostrongyloidea , Tricostrongiloidiasis/veterinaria , Animales , Castración , Bovinos , Coccidiosis/complicaciones , Coccidiosis/patología , Masculino , Pepsinógenos/sangre , Tricostrongiloidiasis/complicaciones , Tricostrongiloidiasis/patología , Pérdida de PesoRESUMEN
The reindeer husbandry range of Scandinavia overlaps with sheep, goat, and cattle pastures. The aim of this study was to determine whether reindeer are suitable hosts for ovine or bovine nematode parasites, and thus may spread these parasites into the reindeer husbandry regions. To render worm-free, twelve 4-month-old male reindeer calves, six lambs, and six bovine calves were given ivermectin at 200 microg/kg body weight. Five weeks post-treatment, six reindeer calves were each artificially dosed with 10,000 third-stage larvae (L3) of gastrointestinal nematodes derived from sheep, and an additional six reindeer with L3 derived from cattle. Lambs and bovine calves received the same dose of ovine and bovine larvae as reindeer, from the same larval source, respectively. Faecal samples collected on five occasions after the larval dosing revealed that by the fourth week, all reindeer calves, lambs, and bovine calves were infected. Animals were slaughtered on days 40 (reindeer) or 47 (lambs and bovine calves) after the larval dosing. Reindeer calves were most susceptible to L3 derived from sheep. The overall mean intensity of Haemochus contortus, Trichostrongylus axei, and Teladorsagia circumcincta, did not differ between reindeer and sheep; however, early fourth-stage larvae of H. contortus were more abundant in reindeer (p = 0.002). The establishment of bovine-derived Ostertagia ostertagi was similar in reindeer (62%) and bovine calves (57%), but larval inhibition was much higher in reindeer (91%, p < 0.001) than in cattle (31%). Very poor establishment of bovine derived Cooperia oncophora was recorded in reindeer calves (2%) compared with bovine calves (59%). These results show that young reindeer are susceptible hosts to the important gastrointestinal parasites of sheep (T. circumcincta, H. contortus) and cattle (O. ostertagi), as well as being a suitable host for T. axei.
Asunto(s)
Animales Salvajes/parasitología , Enfermedades de los Bovinos/transmisión , Reservorios de Enfermedades/veterinaria , Nematodos/patogenicidad , Infecciones por Nematodos/veterinaria , Reno/parasitología , Enfermedades de las Ovejas/transmisión , Animales , Bovinos , Enfermedades de los Bovinos/parasitología , Susceptibilidad a Enfermedades/veterinaria , Heces/parasitología , Masculino , Infecciones por Nematodos/epidemiología , Infecciones por Nematodos/parasitología , Infecciones por Nematodos/transmisión , Oocistos , Ovinos , Enfermedades de las Ovejas/parasitologíaRESUMEN
Previous studies have shown that between-year transmission of Haemonchus contortus in Swedish sheep flocks is almost entirely as over-wintered populations within housed animals, and not on pasture. This suggests that eradication of this parasite is a realistic possibility. Thus, two sheep farms with a history of H. contortus infection on the Swedish island of Oland were selected for study. During the winter housing period of 2003/2004 all ruminants (sheep and cattle) on both farms were treated with ivermectin. Monitoring by faecal egg counts and infective larval differentials of ewes and lambs for the subsequent two grazing seasons, together with total abomasal worm counts of 10 lambs from each farm at the end of the first grazing year, showed that this objective was achieved.
Asunto(s)
Antiparasitarios/uso terapéutico , Hemoncosis/veterinaria , Ivermectina/uso terapéutico , Enfermedades de las Ovejas/tratamiento farmacológico , Animales , Animales Recién Nacidos , Heces/parasitología , Femenino , Hemoncosis/tratamiento farmacológico , Hemoncosis/epidemiología , Hemoncosis/transmisión , Haemonchus/efectos de los fármacos , Vivienda para Animales , Masculino , Recuento de Huevos de Parásitos/veterinaria , Estaciones del Año , Ovinos , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/transmisión , Suecia/epidemiología , Resultado del TratamientoRESUMEN
Five complimentary studies were undertaken with the overall aim to examine the ability of free-living stages of Haemonchus contortus to over-winter and tolerate cold stress. Two studies deal with the development and long-term survival of eggs and infective larvae of two geographically different isolates (Kenya and Sweden). Eggs and larvae were monitored in climatic chambers at temperatures that fluctuated daily between -1 degrees C and 15 degrees C, or at constant temperatures of 5 degrees C and 15 degrees C. The development from egg to larvae was dependent on temperatures over 5 degrees C. The long time survival was favoured at lower temperatures. Furthermore, the overwintering capacity of the free-living stages of these isolates was estimated under Swedish field conditions. Two groups of lambs were experimentally infected with different isolates, and kept separated on previously ungrazed plots. In early May the following year, two parasite-naive tracer lambs were turned out on each of the plots to estimate the pick up of overwintered larvae. This experiment was replicated in central and southern Sweden. In addition, two experiments were performed in 2003 on pasture previously grazed by naturally infected sheep. One trial was on a pasture in southern Sweden grazed by a commercial flock, where extreme numbers of H. contortus were found towards the end of the grazing season 2002. The other study was on a pasture plot in central Sweden grazed by a hobby flock in 2002, where three of six lambs died due to haemonchiasis. Overwintered H. contortus was recorded on three of four experimental sites. Worm burdens were in all instances extremely low. No differences in development and survival were found between the isolates. Consequently, overwintering on pasture is of no practical significance in the transmission of H. contortus between grazing-seasons in Sweden.