Occurrence of GI parasites in ruminants of Kashmir and Ladakh.

The study was conducted in Kashmir and Ladakh Divisions of the erstwhile state of Jammu and Kashmir to work out the occurrence of gastrointestinal parasites in ruminants. The qualitative faecal examination of 476 samples revealed an overall prevalence of GI parasites to the tune of 80.04% comprising of 80.61% in cattle, 96.70% in sheep and 66.27% in goats. In Kashmir overall prevalence of GI parasites was 88.33% comprising of 80.61% in cattle, 98.41% in sheep and 100% in goats. Highest prevalence was observed for strongyles (84.00%) followed by Eimeria spp. (31.67%), Moniezia spp. (20.00%), Ascaris spp. (5.33%) and Strongyloides spp. (1.67%). Mixed infection was observed in 37.67% samples. In cattle, the parasites observed were strongyles (68.37%) and Eimeria spp. (13.27%) with mixed infection in 1.02% samples. In sheep highest prevalence was observed for strongylid eggs (97.88%) followed by Eimeria spp. (43.38%), Moniezia spp. (31.74%), Ascaris spp. (8.47%) and Strongyloides spp. (2.64%) with mixed infection in 59.26% samples. Amongst goats, only strongylid eggs (100%) were observed. In Ladakh region overall prevalence of GI parasites was 65.91% with 82.61% in sheep and 63.40% in pashmina goats. Highest prevalence was observed for Eimeria spp. (63.64%) followed by strongyles (9.09%) Moniezia spp. (5.11%) and Thysanosoma spp. (1.14%). Mixed infection was observed in 13.07% samples. In sheep, highest prevalence was observed for Eimeria spp. (73.91%) followed by strongyles (17.39%) and Thysanosoma spp. (8.70%) with mixed infection in 17.39% samples. In pashmina goats, the prevalence of Eimeria spp. (62.09%) was observed highest followed by strongylid eggs (7.84%) and Moniezia spp. (5.88%) with mixed infection in 12.42% samples.

Multiple anthelmintic resistance in gastrointestinal nematodes of Caprines on Mountain Research Centre for Sheep and Goat at Kashmir Valley, India.

The study was conducted to evaluate the status of anthelmintic resistance in Gastro-Intestinal Nematodes (GINs) of goats at an organized farm located in Kashmir, as there is no report of resistance against these parasites of goats from this temperate region, although it has been reported worldwide including India. Caprines reared at this farm exhibited reduced efficacy to multiple anthelmintics following treatments with Fenbendazole (FBZ), Closantel and Ivermectin (IVM) in Faecal Egg Count Reduction Test (FECRT). The results suggested that the overall efficacy was highest for IVM at 83.5% and 90.0% on 7th and 14th day post-treatment, respectively and least for FBZ at 44.3% and 62.5%, respectively, whereas the corresponding figures for closantel were 68.3% and 86.2%, respectively. The pre-treatment faecal culture revealed Haemonchus contortus, Teladorsagia circumcincta and Trichostrongylus colubriformis as predominant strongyles, however, in post-treatment samples, only H. contortus was observed. Further, the infective larvae were subjected to Allele specific PCR (AS-PCR) for accurate diagnosis of BZ resistance. The AS-PCR revealed 52% of H. contortus were homozygous resistant (rr) and 17% were heterozygous (rS) on day "0" before treatment and 100% homozygous resistant (rr) on 7th day post treatment. In both T. colubriformis and T. circumcincta, 100% population was homozygous susceptible (SS) at day "0" before treatment. The overall frequency of resistant (r) allele for H. contortus was 60.5% and for susceptible allele (s) was 39.5%. For T. colubriformis and T. circumcincta the frequency of susceptible allele (s) was 100%. The survey indicated that the GINs of goats on the farm have developed multiple anthelmintic resistance to FBZ, closantel and IVM and the condition is alarming in the farm. Moreover surveillance studies about status of anthelmintic resistance in other farms (Govternment as well as Private) of Kashmir valley should be carried out at large scale to develop effective and sustainable control strategies against GI Nematodes.

Efficacy of closantel, fenbendazole and ivermectin against GI helminths of cattle in central Kashmir.

Three trials were conducted to evaluate the therapeutic efficacy of closantel (@ 7.5 mg/kg body weight, orally), fenbendazole (@ 7.5 mg/kg body weight, orally) and ivermectin bolus (@ 0.2 mg/kg body weight, orally) against gastrointestinal (GI) helminth parasites of cattle in central zone of Kashmir valley. Twenty cattle suffering from GI helminth infection were randomly selected and divided into two groups in each trial. In each trial, one group comprising of fifteen animals were treated with anthelmintic and second group comprising of five animals were kept as infected untreated control. To determine the therapeutic efficacy of different anthelmintic drugs against GI helminths, faecal samples were directly collected from rectum before treatment and on 7th, 14th and 28th day post treatment and eggs per gram of faeces was determined by using Stoll's method. Then the efficacy of different anthelmintics was calculated by employing Faecal Egg Count Reduction Test. On 14th day post treatment closantel was found to be 100 and 94.44% effective against Fasciola spp. and strongyle worms, respectively, however, it was not found effective against Trichuris spp. The efficacy of fenbendazole was 100% against Fasciola spp., paramphistomes, Moniezia spp., Trichuris spp. and strongyle worms on 14th day post treatment. Ivermectin was found to be 100% effective against Trichuris spp. and strongyle worms on 14th day post treatment, however, it was not effective against Fasciola spp. and paramphistomes.

Prevalence of caprine GI helminths in temperate areas of Jammu & Kashmir.

Prevalence study on GastroIntestinal (GI) helminths of goats was carried out at Govt. Farms of twin districts of Bandipora and Ganderbal and locally reared goats of district Baramulla of the Kashmir Valley over a period of one year from December 2011 to November 2012. A total of 1016 faecal samples were examined which revealed overall prevalence of GI helminths to be 74.70%, with platyhelminths and nemathelminths in 14.76 and 70.07% animals, respectively. The helminths recorded in the present study were Fasciola spp. (2.75%), Dicrocoelium spp. (3.44%), paramphistomes (3.05%), Moniezia spp. (9.44%), strongyle worms including Nematodirus spp. (68.30%), Strongyloides spp. (5.90%) and Trichuris spp. (4.29%). On coprocultural examination Haemonchus spp. (71.05%) was found to be the most predominant strongyle worm followed by Trichostrongylus spp. (12.86%), Chabertia spp. (8.47%) and Ostertagia spp. (7.62%). Seasonal prevalence of GI helminths was observed highest in summer (78.03%) followed by spring (75.39%), winter (74.90%) and autumn (70.47%), the difference being statistically non-significant (P > 0.05). The prevalence of platyhelminths was found to be non-significantly higher in winter (20.00%) followed by spring (15.07%), summer (12.54%) and autumn (11.41%). Nemathelminths were recorded highest in summer (75.68%) followed by spring (71.82%), autumn (68.50%) and winter (64.31%). EPG ranged from 0 to 1500 and an average EPG count was found to be 312.7 ± 17.76. EPG was found to be highest in summer (453.80 ± 45.25) followed by spring (313.2 ± 28.76), autumn (256.1 ± 17.48) and winter (190.2 ± 32.0).Overall prevalence of GI helminths was found more in adult goats (77.85%) compared to young ones (67.42%), the difference being statistically significant (P < 0.05). Similarly, it was found to be higher in females (75.89%) as compared to males (66.12%) and the variation being statistically non-significant (P > 0.05).

Efficacy of ivermectin, closantel and fenbendazole against gastrointestinal nematodes of sheep in Kashmir valley.

The present work was undertaken to evaluate the therapeutic efficacy of ivermectin, closantel and fenbendazole under field conditions against Gastrointestinal Nematodes (GIN) of cross bred merino sheep in Budgam area of Kashmir Valley. A total of 115 sheep having Egg per gram of faeces (EPG) greater than or equal to 150 (mean EPG 258.89) were selected. The animals were randomly divided into four groups comprising of 30 animals each in three treatment groups (ivermectin, closantel and fenbendazole) and twenty-five in fourth untreated infected control group. Faecal samples from the selected animals were collected on day '0' pre treatment and on days 8th and 14th post treatment. Based on Faecal Egg Count Reduction Test (FECRT), ivermectin was found to be 98.80 % effective against strongyles on 8th day post treatment, however an efficacy of 100 % was seen against strongyle worms on 14th day post treatment. 98.80 and 100 % efficacy was observed on day 8th post treatment against strongyles in case of closantel and fenbendazole respectively, however efficacy decreased to 97.60 and 98.8 % respectively on 14th day post treatment. There was no evidence of development of resistance by GIN of cross bred merino sheep in District Budgam of Kashmir Valley to ivermectin, closantel and fenbendazole.

Integrated parasite management with special reference to gastro-intestinal nematodes.

Domestic animals are susceptible to a large number of parasitic diseases, which lead to severe economic losses to livestock industry. So, it is necessary to control parasitic infections in these animals. Control of these helminths is undertaken mostly by anthelmintics, but because of their widespread use there is development of resistance across the globe. However, total dependence on a single method of control has proved to be non-sustainable and cost ineffective in the long term. A combination of treatment and management is necessary to control parasitism so that it will not cause further economic losses to producer as well as to livestock industry. To become practically and ecologically sustainable, parasitic control schemes need to be based on integrated parasite management.

Oestrus ovis larvae in nasal cavity of sheep: a case report.

The present communication reports the infestation of nasal cavities of sheep by larvae of Oestrus ovis from Kashmir Valley.

Toxocara vitulorum in a suckling calf: a case study.

The present clinical case reports toxocariosis in a suckling calf and its management. One and a half month old crossbred Jersey calf was presented at a Veterinary Clinics, District Pulwama, Kashmir with a history of intermittent diarrhea, poor growth, weakness, moderate dehydration and anorexia. On inspection, the calf showed normal physiological parameters viz; body temperature, pulse rate and respiration rate. On faecal examination, eggs of Toxocara vitulorum were found. The animal was treated with 30 ml suspension of 1.5 % fenbendazole + 0.5 % praziquantel orally. After examination of faecal samples on 7th and 14th day post treatment no T. vitulorum eggs were observed.

Cytotoxic Potential and Molecular Characterization of Fungal Endophytes from Selected High Value Medicinal Plants of the Kashmir Valley - India.

The present study explores the fungal endophytes from selected high value medicinal plants to check their activities at in-vitro and in-vivo level. The in-vitro cytotoxicity of selected endophytes revealed potent growth inhibition against human cancer cell lines of leukemia (THP-1), lung (A549), prostate (PC-3), colon (Caco-2), neuroblastoma (IMR-32) and breast (MCF-7) at a concentration of 100 µg/ml. Among them the endophytic strains I. e., IIIM2, IIIM3, IIIM7 and IIIM8 showed most significant growth inhibition against colon (Caco-2), prostate (PC-3), lung (A549) and leukemia (THP-1) cancer cell lines. At the in-vivo level maximum (58.95%) tumor growth inhibition was documented with the extract of IIIM2 against Ehrlich Ascites Carcinoma mouse modal. All the potent fungal endophytic strains were characterized using ITS 4 and ITS 5 region sequencing and phylogenetic analysis was ascertained among them. This paper confirms the 2 elite endophytic fungal strains, IIIM2 and IIIM8, have the potential to act as a source of new anticancer compounds.

SIRS at Admission Is a Predictor of AKI Development and Mortality in Hospitalized Patients with Severe Alcoholic Hepatitis.

BACKGROUND: Systemic inflammatory response syndrome (SIRS) is associated with an increased risk of hepatic encephalopathy, renal failure, and poor outcome in patients with cirrhosis; however, there is a paucity of studies on this entity for severe alcoholic hepatitis (SAH). AIM: To evaluate SIRS at baseline as a predictor of development of acute kidney injury (AKI) and mortality in patients with SAH. METHODS: Consecutive in-patients with SAH (discriminant function ≥ 32) without AKI at baseline were followed up for the development and progression of AKI (AKIN criteria). RESULTS: Of the 365 patients (mean age 45.5 ± 9.5, 356 males), SIRS at baseline was present in 236 (64.6%). AKI developed in 122 (33.4%), of which 50 (40.9%) had progression of AKI. SIRS was associated with bacterial infections in 96 (40.6%) and in 140 (59.3%) occurred in the absence of proven infection microbiologically. The presence of SIRS predicted both AKI development (p < 0.001, OR 2.9, 95% CI 1.7-4.8) and AKI progression (p = 0.002, OR 3.27, 95% CI 1.48-7.21). Resolution of AKI also had a significant inverse association with SIRS (p = 0.001). High MELD score (p = 0.002, HR 1.1, 95% CI 1.02-1.09), in-hospital progression of AKI (p = 0.04, HR 1.54, 95% CI 1.003-2.38), and SIRS (p = 0.004, HR 1.98, 95% CI 1.25-3.1) were significant predictors of 90-day mortality (model 1), while high MELD score (p < 0.001, HR 1.1, 95% CI 1.04-1.12) and bacterial infections (p = 0.001, HR 1.8, 95% CI 1.27-2.6) were independent predictors of mortality in the second multivariate model (model 2). CONCLUSION: SIRS at admission predicts both the development of AKI and 90-day mortality in patients with SAH. This could definitely have a therapeutic and prognostic implication.

Dipylidium caninum infection in dogs infested with fleas.

The present study pertains to the Dipylidium caninum infection in dogs infested with fleas. Twenty dogs were presented to the Divison of Surgery, SKUAST-K for different surgical procedures. Majority of the dogs had a history of pruritus, loss of weight as well as rubbing their perineal region against the wall. On external examination dogs were found infested with Ctenocephalides canis. When dogs were anesthetized, motile segments were seen coming out of their anus, which were then identified as mature segments of D. caninum.

Prevalence of gastrointestinal helminth infections in ovine population of Kashmir Valley.

AIM: Gastrointestinal (GI) helminth parasitism is one of the major constraints for profitable sheep production. Due to variations in the prevalence of GI helminths from region to region, it becomes important to map out accurately the parasitic fauna of a particular region for development of suitable control measures. MATERIALS AND METHODS: An extensive study of GI helminths was carried out in Budgam district of Kashmir Valley over a period of 1 year. A total of 1200 fresh ovine faecal samples from both sexes of young ones and adults were collected in sterilized plastic bags and examined by standard sedimentation and floatation techniques. Positive faecal samples (15-20%) in each season were examined by Stoll's dilution method to determine the parasitic load. A total of 120 faecal samples (30 samples in each season) positive for strongyle eggs were subjected to coproculture using Petridish method and the third stage larvae were harvested to find out prevalence of different genera of strongyle worms. RESULTS: The overall prevalence of GI helminths was found to be 77% with platyhelminths and nemathelminthes in 26.58 and 60.92% animals, respectively. The overall prevalence of mixed GI helminths was found to be 8.67%. Eggs of various helminths encountered in the present study were those of Fasciola spp. (3.58%), Dicrocoelium spp. (11.58%), paramphistomes (4.83%), Moniezia spp. (7.92%), strongyle worms including Nematodirus spp. (57.75%), Strongyloides spp. (1.67%), and Trichuris spp. (1.5%). On coprocultural examination Haemonchus spp. (55%) was found to be most predominant strongyle worm followed by Trichostrongylus spp. (17.5%), Ostertagia spp. (11.67%), Oesophagostomum spp. (9.17%), and Chabertia spp. (6.67%). On seasonal basis, highest prevalence of GI helminths was recorded in summer (83.00%) followed by spring (78.67%), winter (76.33%), and autumn (70.00%), the difference being statistically non-significant (p>0.05). The prevalence of platyhelminths (Fasciola spp., Dicrocoelium spp. and Moniezia spp.) was found to be non-significantly higher in winter, but paramphistomes showed the highest prevalence in the summer season. Nemathelminth infection was found highest in summer season and lowest during the winter season. Eggs per gram (EPG) ranged from 0 to 1800, and an average EPG count was found to be 454.35±27.85. EPG was found to be highest in summer (684.00±69.83) and lowest in winter (202.38±18.82). The overall prevalence of GI helminths was found more in adult sheep (83.00%) compared to young ones (53.11%), the difference being statistically significant (p<0.05). Similarly, the prevalence of helminths was found to be higher in females (78.32%) as compared to males (72.97%), the variation being statistically non-significant (p>0.05). CONCLUSION: Seasonal variation plays an important role in the prevalence of GI helminths in addition to age and sex of the animal.

Evaluation of selected honey and one of its phenolic constituent eugenol against L1210 lymphoid leukemia.

People affected with leukemia are on the rise and several strategies were employed to thwart this deadly disease. Recent decade of research focuses on phenolic constituents as a tool for combating various inflammatory, cancer, and cardiac diseases. Our research showed honey and its phenolic constituents as crusaders against cancer. In this work, we explored the antileukemic activity of selected honey and one of its phenolic constituent eugenol against L1210 leukemia animal model. Results of this experiment showed that the selected honey samples as well as eugenol after intraperitoneal injection could not increase the median survival time (MST) of animals. Further, there was only slight marginal increase in the %T/C values of honey and eugenol treated groups. The number of phenolics present in the honey may not be a prime factor to promote antileukemic effect since there was no difference in the MST of two different honeys tested. This study limits the use of selected honey and eugenol against leukemia animal model.

Anticancer activity, toxicity and pharmacokinetic profile of an indanone derivative.

The present study describes anticancer effect of gallic acid based indanone derivative (1). Indanone 1 exhibited in vivo anticancer activity against Erhlich ascites carcinoma in Swiss albino mice by inhibiting tumor growth by 54.3% at 50 mg/kg b.wt. It showed antitubulin effect by inhibiting tubulin polymerase enzyme. In cell cycle analysis, it inhibited G2/M phase and induced apoptosis. It significantly suppressed VEGF-R1, VEGF-R2 and HIF-α in human breast cancer MCF-7 cells, thus exhibiting antiangiogenic activity. In acute oral toxicity, indanone 1 was well tolerated and was found to be non-toxic up to 1000 mg/kg b.wt. in Swiss albino mice. Pharmacokinetic studies in rabbits revealed rate of absorption, half life, volume of distribution with high plasma and blood clearance after i.v. administration. Indanone 1, is a safe and moderately active anticancer agent.

Intramuscular cavernous haemangioma of the triceps.

Haemangiomas are one of the most common soft tissue tumours comprising 7% of all benign tumours. Vascular malformations are often confused with haemangiomas. The etiology is unknown. They are common in infancy and childhood and females are more commonly affected. These tumours may be superficial or deep, and deeply seated lesions, are difficult to diagnose clinically and hence require radiographic assessment. Deep-seated haemangiomas are usually intramuscular, although intra-articular synovial haemangiomas also occur. The commonest anatomic site is the lower limb.Despite their vascular origin, haemangiomas do not metastasize or undergo malignant transformation. Many treatment modalities for the symptomatic haemangioma are available but surgical excision is the preferred treatment. We present an unusual case of a dumb-bell intramuscular haemangioma involving the triceps and extending into the cubital tunnel of the elbow, distinguish between haemangiomas and vascular malformations and emphasize the importance of surgical technique in ensuring ulnar nerve safety.

A cyano analogue of boswellic acid induces crosstalk between p53/PUMA/Bax and telomerase that stages the human papillomavirus type 18 positive HeLa cells to apoptotic death.

The p53 tumor suppressor pathway is disrupted by human papillomavirus (HPV) in over 90% of cervical cancers. HPV E6 protein promotes the degradation of p53 thereby inhibiting its stabilization and activation. This study demonstrates that treatment with a novel cyano derivative of 11-keto-ß-boswellic acid, i.e. butyl 2-cyano-3, 11-dioxours-1,12-dien-24-oate (BCDD) reduced the viral E6 mRNA expression and lead to the accumulation of transcriptionally active p53 in the nucleus of HPV18 HeLa cells following DNA damage. Western blot analysis showed that BCDD robustly up regulated time-dependent expression of p53/PUMA/p21 whereas it deprived cells essentially of p-AKT and NF-κB cell survival signalling cascade. BCDD appeared to gear up PUMA activation through p53 pathway and that both p53 and p21 translocated heavily into the nucleus. Simultaneously, it inhibited anti-apoptotic Bcl-2, augumented Drp-1 expression, disrupted mitochondrial functions causing the activation of proapoptotic proteins and caspases activation. Additionally, BCDD inhibited telomerase expression that's likely to result in a marked reduction of the tumorigenic potential of high-grade cervical cancers. Consequently BCDD caused apoptotic death in cervical cancer cells as evidenced by DNA fragmentation and PARP-cleavage. Further, BCDD did not affect the extrinsic signalling transduction pathway as depicted by its null effect on caspase-8. The in vivo anticancer activity of BCDD was investigated in Ehrlich Ascites carcinoma model where it exhibited tumor regression by 48% at 30 mg/kg, i.p., in mice. These findings indicated that BCDD is a potential candidate that may be found useful in the management of cervical cancer.

Effect of honey and eugenol on Ehrlich ascites and solid carcinoma.

Ehrlich ascites carcinoma is a spontaneous murine mammary adenocarcinoma adapted to ascites form and carried in outbred mice by serial intraperitoneal (i/p) passages. The previous work from our laboratory showed that honey having higher phenolic content was potent in inhibiting colon cancer cell proliferation. In this work, we extended our research to screen the antitumor activity of two selected honey samples and eugenol (one of the phenolic constituents of honey) against murine Ehrlich ascites and solid carcinoma models. Honey containing higher phenolic content was found to significantly inhibit the growth of Ehrlich ascites carcinoma as compared to other samples. When honey containing higher phenolic content was given at 25% (volume/volume) intraperitoneally (i/p), the maximum tumor growth inhibition was found to be 39.98%. However, honey was found to be less potent in inhibiting the growth of Ehrlich solid carcinoma. On the other hand, eugenol at a dose of 100 mg/kg i/p was able to inhibit the growth of Ehrlich ascites by 28.88%. In case of solid carcinoma, eugenol (100 mg/kg; i/p) showed 24.35% tumor growth inhibition. This work will promote the development of honey and eugenol as promising candidates in cancer chemoprevention.