Asunto(s)
Cateterismo Periférico , Activadores Plasminogénicos/uso terapéutico , Terapia Trombolítica/métodos , Activador de Tejido Plasminógeno/uso terapéutico , Activador de Plasminógeno de Tipo Uroquinasa/uso terapéutico , Arteriopatías Oclusivas/tratamiento farmacológico , Oclusión de Injerto Vascular/tratamiento farmacológico , Humanos , Infusiones Intravenosas , Activadores Plasminogénicos/administración & dosificación , Activador de Tejido Plasminógeno/administración & dosificación , Estados Unidos , Activador de Plasminógeno de Tipo Uroquinasa/administración & dosificación , Trombosis de la Vena/tratamiento farmacológicoRESUMEN
GI1147211 is a 7-substituted 10,11-ethylenedioxy-20(S)-camptothecin analogue that inhibits the nuclear enzyme topoisomerase I. In this Phase I and pharmacological study, 24 patients with advanced solid malignancies received a total of 72 courses of GI147211 as a 30-min infusion daily for 5 consecutive days, at doses ranging from 0.3 to 1.75 mg/m2/day. Severe neutropenia precluded dose escalation above 1.5 mg/m2/day in minimally pretreated patients, and both severe neutropenia and thrombocytopenia were dose limiting in heavily pretreated patients at doses above 1.0 mg/m2/day. These doses are, therefore, recommended for subsequent Phase II evaluations of GI147211 in patients with comparable prior therapy. Nonhematological toxicities, including nausea, vomiting, fatigue, and anorexia, were mild to moderate. The disposition of GI147211 in blood was described by a linear three-compartment model, with renal elimination accounting for only 11% of drug distribution. No relationship was observed between the pharmacological exposure to GI147211 and effects on neutrophils; however, patients who developed dose-limiting myelosuppression did experience greater exposure to both the lactone and total forms of the drug. The hydrolysis kinetics of GI147211 revealed not only a shift of the drug to the inactive carboxylate form in human serum albumin but also stabilization of the lactone in erythrocytes, perhaps accounting for the observed lactone:total area under the concentration-time curve ratio of 0.27. These results indicate that GI147211 exhibits predictable toxicities and that further studies are warranted to determine the distinct role of this compound among currently available camptothecin analogues.
Asunto(s)
Antineoplásicos/efectos adversos , Antineoplásicos/farmacocinética , Camptotecina/análogos & derivados , Neoplasias/tratamiento farmacológico , Adulto , Anciano , Antineoplásicos/administración & dosificación , Camptotecina/administración & dosificación , Camptotecina/efectos adversos , Camptotecina/farmacocinética , Esquema de Medicación , Femenino , Humanos , Infusiones Intravenosas , Masculino , Persona de Mediana Edad , Neoplasias/sangre , Neutropenia/inducido químicamente , Trombocitopenia/inducido químicamenteRESUMEN
BACKGROUND & AIMS: A prospective double-blinded study with preset sonographic criteria has not been performed to assess the accuracy of duplex ultrasonography in determining the patency of transjugular intrahepatic portosystemic shunts (TIPS). The purpose of this study was to determine the sensitivity and specificity of duplex ultrasonography in predicting shunt malfunction using accepted preset sonographic criteria. METHODS: Sixty ultrasonographic and venographic follow-up comparisons were made on 38 cirrhotic patients who had undergone TIPS placement for variceal bleeding (n = 28) or intractable ascites (n = 10). Ultrasonographic results were analyzed by one of two board-certified ultrasonographers without knowledge of venographic findings. RESULTS: Of the 31 occluded (n = 8) and stenotic (n = 23) shunts, ultrasonography accurately predicted a shunt malfunction (occlusion or stenosis) in only 11 studies and incorrectly predicted patency in 20. Compared with venography, ultrasonography had a sensitivity of 35% and a specificity of 83% in predicting TIPS stenosis or occlusion. CONCLUSIONS: These results suggest that duplex sonography is not a sensitive test in predicting the presence of a hemodynamically significant stenosis and that shunt status should be assessed by venography and direct portal pressure measurements until a more reliable and proven noninvasive ultrasonographic criterion is devised.
Asunto(s)
Derivación Portosistémica Intrahepática Transyugular , Ultrasonografía Doppler Dúplex , Grado de Desobstrucción Vascular , Adulto , Método Doble Ciego , Femenino , Predicción , Humanos , Masculino , Persona de Mediana Edad , Flebografía , Estudios Prospectivos , Sensibilidad y Especificidad , Grado de Desobstrucción Vascular/fisiologíaRESUMEN
OBJECTIVES: This study examined condom use in legal Nevada brothels. METHODS: Forty female prostitutes in two brothels were interviewed about client resistance to condoms and techniques for facilitating condom use. RESULTS: Of 3290 clients in the previous month, 2.7% (95% confidence interval [CI] = 2.2%,3.4%) were reluctant to use condoms. Of these individuals, 72% ultimately used condoms, while 12% chose nonpenetrative sex without condoms. The remaining 16% left the brothels without services. Condom use rates were markedly lower with nonpaying sex partners (lowers) than with clients. CONCLUSIONS: Brothel prostitutes may be at greater risk for acquiring HIV and other sexually transmitted diseases from lovers than from clients.
Asunto(s)
Condones , Conocimientos, Actitudes y Práctica en Salud , Comunicación Persuasiva , Trabajo Sexual/psicología , Parejas Sexuales/psicología , Adulto , Empleo/legislación & jurisprudencia , Empleo/estadística & datos numéricos , Femenino , Humanos , Masculino , Estado Civil , Persona de Mediana Edad , Nevada , Factores de Riesgo , Trabajo Sexual/legislación & jurisprudencia , Enfermedades de Transmisión Sexual/prevención & control , Encuestas y CuestionariosAsunto(s)
Condones , Anticoncepción , Falla de Equipo , Femenino , Humanos , Masculino , Enfermedades de Transmisión SexualRESUMEN
The well documented hydrolysis of the alpha-hydroxy-delta-lactone ring moiety in camptothecin and related analogues is routinely monitored using high-performance liquid chromatography (HPLC). Previous HPLC separations of the lactone and carboxylate forms of camptothecins have often required mobile phases containing three to four components; ion-pairing reagent to provide adequate retention of the carboxylate form of the drug; buffer to control the ionic strength and pH of the mobile phase; acetonitrile to control the retention of the lactone form and, in some instances, sodium dodecyl sulfate to reduce peak tailing. Because of the complexity of the mobile phases employed, development of these assays can be a laborious process, requiring re-optimization for each new analogue. In this study, we have developed a simple HPLC methodology for the simultaneous separation of the lactone and carboxylate forms of numerous camptothecin analogues. The mobile phase employed includes only triethylamine acetate (TEAA) buffer and acetonitrile. In this application, triethylamine serves multiple roles; as the ion-pairing reagent, as a masking agent for underivatized silanols and as the major buffer component. By altering only the composition of TEAA buffer with respect to acetonitrile, method development becomes a more streamlined and time efficient process. In this publication, we present the simultaneous separation of the lactone and carboxylate forms of camptothecin and four related analogues, namely, topotecan, GI147211, 10-aminocamptothecin and the CPT-11-SN-38 drug-metabolite pair. It is proposed that this new mobile phase, consisting of only triethylamine acetate buffer and acetonitrile, can be used for the analysis of the several camptothecin derivatives presently in clinical trials as well as the numerous other analogues in preclinical development.
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Antineoplásicos Fitogénicos/sangre , Camptotecina/sangre , Ácidos Carboxílicos/sangre , Cromatografía Líquida de Alta Presión/métodos , Lactonas/sangre , Acetonitrilos , Tampones (Química) , Camptotecina/análogos & derivados , Etilaminas , Humanos , Concentración de Iones de Hidrógeno , Irinotecán , Concentración Osmolar , TopotecanRESUMEN
BACKGROUND: The purpose of the present study was to examine the histological consequences of the endoluminal exclusion of blood flow effected with stent graft technology. METHODS AND RESULTS: In 25-kg mongrel dogs, patulous vein patch infrarenal aortoplasty with iliac vein produces a fusiform abdominal aortic dilation (AAD). All aortic tributaries were preserved. Endoluminal exclusion via transfemoral placement of a thin-wall Dacron graft occurred 4 +/- 2 months later (n = 23). Balloon-expandable stents anchored the ends of the graft to the aorta. Hematoxylin and eosin, elastin van Gieson's, and Masson's trichrome staining was performed 6 and 12 months later at death. In control nongrafted AADs, the arterial portion of the AAD was lined by elastin -and collagen-rich intimal hyperplasia, and the venous portion developed medial hyperplasia containing collagen but little elastin. After stent graft placement, the stent struts and the graft were completely incorporated into an elastin-poor, collagen-rich neointima. Fibrosis of the vein patch was observed at 1 year. laminated thrombus did not form in the AAD until immediately after stent graft placement; flow arrest occurred in the space between the graft and the AAD intima despite the patent tributaries. At 6 and 12 months, microscopic recanalization was seen in this thrombus, although macroscopic flow was not discernible by duplex imaging or angiography. No AAD growth was measured. CONCLUSIONS: Aortic dilation was not observed at 1 year after stent graft placement within AADs with patent side branches despite microscopic evidence of thrombus recanalization. A collagen-rich and elastin-poor neointima incorporated the entire stent graft.
Asunto(s)
Aorta Abdominal/patología , Stents , Animales , Perros , Músculo Liso Vascular/patologíaRESUMEN
OBJECTIVES: The purpose of this study was to evaluate condom use and the incidence of breakage and slippage during vaginal intercourse among female prostitutes in legal Nevada brothels, where use of condoms is required by law. METHODS: Forty-one licensed prostitutes in three brothels were enrolled in a prospective trial in August 1993. Used condoms were collected to verify reported breaks visually. Retrospective breakage and slippage rates were obtained in a standardized interview. RESULTS: Condoms were used for every act of vaginal intercourse with a brothel client during the study period, as well as in the previous year. In the prospective study phase, condoms were used in 353 acts of vaginal intercourse with clients. No condoms broke, and none fell off the penis during intercourse. Only twice (0.6%) did condoms completely fall off during withdrawal. Twelve times (3.4%) during intercourse and 15 times (4.3%) during withdrawal, condoms slipped down the penis but did not fall off. CONCLUSIONS: These findings, among the lowest breakage and slippage rates published, suggest that regular condom use may lead to condom mastery and the development of techniques to reduce the likelihood of breakage and slippage.
Asunto(s)
Condones/estadística & datos numéricos , Trabajo Sexual , Adulto , Falla de Equipo , Femenino , Humanos , Masculino , Nevada , Estudios Prospectivos , Proyectos de Investigación , SexoRESUMEN
Healthy children, 1.5 to seven years old, were divided into three groups of 20 each. Group 1 received midazolam 0.5 mg.kg-1, Group 2, ketamine 6 mg.kg-1 and Group 3 a mixture of midazolam 0.4 mg.kg-1 + ketamine 4 mg.kg-1. Each dose was mixed with atropine 0.02 mg.kg-1 plus an equal volume of cherry syrup and was given orally 20 to 30 min prior to surgery. A grade of 1 (asleep, difficult to arouse), 2 (asleep, easily aroused), 3 (awake, calm), 4 (awake, anxious, occasional cry), or 5 (crying, agitated), was assigned at the time of parental separation and again when mask induction was begun. A grade of 1-3 was considered successful. For parental separation, the mixture of ketamine+midazolam was 100% successful, ketamine 90% and midazolam 75%. Successful mask induction for the mixture of ketamine+midazolam was 85%, midazolam 65% and ketamine 42%. This study indicates that a mixture of ketamine+midazolam is the most effective.
Asunto(s)
Adyuvantes Anestésicos/administración & dosificación , Anestésicos Disociativos/administración & dosificación , Hipnóticos y Sedantes/administración & dosificación , Ketamina/administración & dosificación , Midazolam/administración & dosificación , Medicación Preanestésica , Administración Oral , Anestesia por Inhalación , Ansiedad/prevención & control , Nivel de Alerta/efectos de los fármacos , Atropina/administración & dosificación , Niño , Preescolar , Llanto , Combinación de Medicamentos , Aromatizantes , Humanos , Lactante , Máscaras , Vehículos Farmacéuticos , Agitación Psicomotora/prevención & control , Sueño/efectos de los fármacos , Vigilia/efectos de los fármacosAsunto(s)
Cateterismo Periférico/instrumentación , Medios de Contraste , Arteria Hepática/diagnóstico por imagen , Vena Porta/diagnóstico por imagen , Derivación Portosistémica Quirúrgica/métodos , Várices Esofágicas y Gástricas/terapia , Femenino , Fluoroscopía , Hemorragia Gastrointestinal/terapia , Venas Hepáticas , Humanos , Persona de Mediana Edad , Derivación Portosistémica Quirúrgica/instrumentación , Radiografía IntervencionalRESUMEN
Barriers and spermicides are being developed for the prevention of unwanted pregnancy and sexually transmitted infection. The inconvenience of current methods limits their acceptability and leads to higher user failure rates. New methods, such as female condoms, nonlatex male condoms, and new cervical caps are being developed: the Reality (Wisconsin Pharmacal, Jackson, WI) polyurethane vaginal pouch has already received conditional Food and Drug Administration approval for use in the United States. The material used in a Food and Drug Administration-approved nonallergenic glove is being tested for its effectiveness as a condom. In addition, the Fem Cap, a cervical cap that had a life table probability of failure within 1 year of 4.8%, is expected to be available within the next 2 years. It is important for clinicians to be informed of new options becoming available for those patients who may be good candidates for these barrier methods.
PIP: Reproductive health providers have long overlooked barrier and spermicide contraceptives, but they are rather effective when used consistently and correctly, have relatively few side effects, and protect against sexually transmitted diseases (STDs) and HIV. The mean percentage of women who have an unplanned pregnancy during the first year, if they have correctly and consistently used condoms is 2%, 3% for spermicides, 6% for cervical cap and diaphragm, 6% for nulliparous users of the sponge, and 9% for parous users of the sponge. If they do not use these methods and/or spermicides properly, however, failure rates increase 3-7 times. New barrier methods and spermicides should help people overcome obstacles, e.g., sensitivity or allergy to latex. One condom manufacturer is developing nonlatex male condoms for those who are sensitive or allergic to latex or the chemicals added to latex during the manufacturing process. Another manufacturer has altered the male condom by adding a silicone-lubricated pouch of latex to fit loosely around the glans penis, thereby reducing sensation loss. A new barrier method is the cervical cap which fits and covers the cervix snugly. Female condoms empower women to protect themselves against pregnancy, STDs, and HIV. They should be available to the US market soon. Some research indicates that spermicide use with barrier methods poses a risk of urinary tract infections, but the benefits exceed the risk. Barrier and spermicide methods appear to protect against cervical cancer and pregnancy complications. In conclusion, reproductive health providers need to realize their role in controlling patient use of barriers and spermicides, as well as the important role barrier methods play in protecting against pregnancy and STDs. Therefore, they should encourage patients to use the new methods and teach them to use them effectively.
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Dispositivos Anticonceptivos/estadística & datos numéricos , Espermicidas/uso terapéutico , Dispositivos Anticonceptivos/efectos adversos , Anticonceptivos Poscoito/uso terapéutico , Estudios de Evaluación como Asunto , Femenino , Humanos , Masculino , Aceptación de la Atención de Salud , Embarazo , Complicaciones del Embarazo/epidemiología , Complicaciones del Embarazo/mortalidad , Embarazo no Deseado/estadística & datos numéricos , Espermicidas/efectos adversos , Infecciones Urinarias/epidemiología , Infecciones Urinarias/etiología , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/mortalidadRESUMEN
A prospective study using two brands of condoms found that of 405 condoms used for intercourse, 7.9% either broke during intercourse or withdrawal or slipped off during intercourse; none of these events were related to condom brand, past condom use or use of additional lubricant. Of the remaining condoms, 7.2% slipped off during withdrawal; slippage was not related to condom brand or past use of condoms, but it was significantly higher when additional lubricant was used.
PIP: The use of the Pleasure Plus condom was compared with Trojan-Enz to determine the breakage and slippage during use by US couples. Factors associated with slippage and breakage are discussed. The comparison indicated that thicker than currently available condoms (standards) were considered more comfortable. The prospective study involved complete data from 68 couples recruited from the Emory University Family Planning Program of which 79% were black, 18% non-hispanic white, and 3% native American or White Hispanic. The woman's mean age was 34.4 years. 25% were 30.3 years and 25% were 39.0 years. Male partner's mean age was 37.7 years, with similar proportions 30 and 42 years. 65% were circumcised males. 26% had not used a condom in the year preceding the study. 21% had never used a condom in their relationship. Among users, average use in the preceding month was 5.8 condoms and in the preceding year, 21.1 condoms. Among condom users, the average breakage rate was 3.1%/couple in the preceding month and 4.7% in the preceding year. These breakage rates were higher than reported rates in the US. Couples were asked to use 3 experimental and 3 standard condoms within a 4-week period. The results of the use of 203 experimental condoms showed that 3 broke during intercourse or withdrawal and 4 broke after the use of 202 standard condoms. Of the 405 condoms used, breakage reported was 1.7% with no significant differences between the 2 types. Those inexperienced had the highest breakage rate of 2 out of 84 condoms (2.4%). Previous users had 1.6% breakage rates. Of the experienced users, those who had reported condom breaks prior to the study experienced higher rates of breakage (2.8%) vs. those who did not (.9%), but the difference was not statistically significant. Slippage was 6.4% for the experimental and 5.9% for the standard condoms. Of those experimental condoms not breaking or falling off during intercourse, 6.4% slipped during withdrawal. Sensitivity ratings for women were 6.7% for the experimental condoms and 5.7% for the standards compared with the male sensitivity ratings of 6.1% and 5.1%, respectively. Ratings were lower among circumcised men. Women rated the experimental condoms at 6.9 and 5.6 for the standards on the average, while men rated them at 6.4 and 5.1, respectively. Some preferred the blue color. It is pointed out that the 14.6% slippage and breakage rate for both condoms is high and creates risk. These method failure and user failure rates are higher than in reported studies. An explanation for the high rates may be lack of user seriousness in the study, or poor wording of questions, and imperfect use with oil-based lubricants. Condom efficacy studies are a high priority given the AIDs and sexually transmitted disease prevention efforts.
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Dispositivos Anticonceptivos Masculinos , Adolescente , Adulto , Femenino , Infecciones por VIH/prevención & control , Infecciones por VIH/transmisión , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Forty-nine mutually monogamous couples used a total of 478 condoms during vaginal intercourse in a prospective trial whose purpose was to discover whether the performance of a new non-latex hypoallergenic condom was substantially equivalent to that of a leading condom brand already being marketed. Of these 478 condoms, seven (1.46%) either broke or fell off the penis during intercourse. Two (0.42%) of the 471 condoms that did not break or fall off during intercourse fell off the penis when it was being withdrawn from the vagina. Altogether, 469 (98.1%) of the 478 condoms used for intercourse survived intact throughout intercourse and withdrawal. Differences in breakage and slippage rates for the two condom brands were statistically insignificant. The overall 98.1% success rate is much higher than the rate of success found in a previous condom trial with nearly identical research protocol. The reason for the difference is attributed to much more precise questioning of subjects in the current trial.
PIP: This comparative study of the Tactylon condom and the Trojan-Enz condom was conducted in order to obtain FDA market approval. 50 female subjects were recruited through public notices for hospital and university personnel. The requirements were that female subjects must be between the ages of 18-55, fertile and not presently using another contraceptive, and not at risk for or have a significant medical history of sexually transmitted disease. Male partners were also to be in good health and currently not using another form of contraception. Couples were to use condoms in 10 acts of intercourse over a 5-week period without a lubricant. Each couple received 5 of each kind of condom and 10 questionnaires, each numbered sequentially from 1 to 10. Condoms and questionnaires were to be used in sequential order beginning with 1. Half the couples started with the Tactylon condom and the other half with Trojan-Enz. Demonstrations and instruction were given for use. Caution is advised in interpreting breakage rates due to the small sample sizes (250 for each brand). 49 of the subject completed the study. 82% were black and 18% were whites, non-Hispanic. 45% were married, 24% single, 27% were divorced, and 4% separated. 14% were nulliparous, 27% with 1 child, 37% with 2 children, and 22% with 3 children. 37% reported incomes of 20,000 or 35,000. The mean age was 34.4 for females and 37.2 for males. 84% of males were circumcised. The average monthly number of acts of intercourse was 14.5 and yearly 147.2. Condoms were used in this study similar to past behavior, with 10 condoms used in 21 days. 45% had not used condoms in the preceding month, and 16% in the preceding year. 10% had never used condoms in their relationship. The average use of condoms for those using in the past month was 7.8. The average breaking rate per condom for those who used condoms in the prior month was 6.2%, and in the prior year 5.0%. The breakage and slippage rates were high. Conditions for breakage and slippage were defined and clearly recorded. Evaluation for pleasure was requested of each party. The results were that breakage and slippage rates were similar for the 2 condoms, and lower than prior use and unrelated to use of a lubricant or prior use conditions. The frequency of slippage necessitates further research. The falling off rate of 3 in 472 condoms was not statistically different from retrospective rates of users. Prior trial rates were higher perhaps due to the condoms being lubricated, or the changed wording on the questionnaire to differentiate falling off. The subject's evaluations of condoms were also similar for both condoms.
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Coito , Dispositivos Anticonceptivos Masculinos/normas , Adulto , Estudios de Evaluación como Asunto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Conducta SexualRESUMEN
A computer program has been developed to assist in the preparation of proper drug and anaesthetic doses for children. An individualized reference sheet containing the doses of the more commonly used drugs for the induction and maintenance of anaesthesia, fluid requirements, blood volume information, ventilatory variables, estimated endotracheal tube sizes and emergency drug doses constitutes the output of this program. The importance of such a computer program in improving paediatric anaesthetic management is discussed.