RESUMEN
During a 16-month period, 10 goats with listeriosis were identified in 2 herds that shared 3 bucks, including 1 that died of listeriosis. Using DNA fingerprinting, we determined that a single genetically unique Listeria monocytogenes strain had infected all goats from which isolates were available. All isolates were unable to metabolize rhamnose (rhamnose-negative), whereas as a species, L monocytogenes is considered to have a rhamnose-positive phenotype. Therefore, these isolates would have been characterized as a species other than L monocytogenes if any of a variety of commercial bacterial identification kits had been used for speciation. Silage was not fed to either herd, and L monocytogenes was not isolated from vaginal or rectal swab specimens obtained from healthy goats or from samples of feed. Because the 3 bucks were the only common elements between the 2 herds, our results suggest a venereal route of transmission for listeriosis.
Asunto(s)
Brotes de Enfermedades/veterinaria , Enfermedades de las Cabras/epidemiología , Listeria monocytogenes/clasificación , Listeriosis/veterinaria , Aborto Veterinario , Animales , Femenino , Gentamicinas/uso terapéutico , Enfermedades de las Cabras/microbiología , Enfermedades de las Cabras/transmisión , Cabras , Listeria monocytogenes/genética , Listeriosis/epidemiología , Listeriosis/microbiología , Masculino , Penicilina G/uso terapéutico , Reacción en Cadena de la Polimerasa/veterinaria , Polimorfismo de Longitud del Fragmento de Restricción , Embarazo , Recto/microbiología , Enfermedades de Transmisión Sexual/epidemiología , Enfermedades de Transmisión Sexual/microbiología , Enfermedades de Transmisión Sexual/veterinaria , Vagina/microbiologíaRESUMEN
The correlation between the antimicrobic standard disk agar diffusion procedure and a veterinary antimicrobic breakpoint minimal inhibitory concentration system was evaluated. Bacterial isolates representing 5 different genera were tested against 15 antimicrobics. There were 3,795 tests performed; 77.3% of the test results were in agreement, 22.6% were in disagreement. Thirty-one percent of the conflicting results were due to the organism being intermediate on the standard disk agar diffusion procedure. Results suggest that the breakpoint system needs more than 1 antimicrobic dilution per antimicrobic, or a change in some of the single dilutions in the breakpoint system would eliminate some of these discrepancies. Another possibility would be to utilize more than 1 panel design, i.e., 1 for gram-positive organisms, 1 for gram-negative organisms, and 1 for fastidious organisms.