RESUMEN
To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 mmol/L, which was 51.17 mol/L when combined with curcumin and random sequence. The IC50 of curcumin was 30.02 mmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins.
Asunto(s)
Inhibición de Migración Celular/efectos de los fármacos , Neoplasias del Colon , Curcumina/farmacología , Neoplasias/prevención & control , ARN , ARN Interferente Pequeño/efectos de los fármacosRESUMEN
Objective: The aim of this study was to evaluate the effect of mucosa suspension procedure (MSP) by comparing procedure for prolapse and hemorrhoids (PPH). Methods: We compared the safety, clinical outcome, and cost between PPH and MSP for the surgical treatment of hemorrhoidal disease. A total of 80 patients with II and III degree hemorrhoids were included in the study. MSP group (n=40) and PPH group (n=40) were applied at random. Results: Both MSP and PPH are safe surgical treatments for patients with II and III degree hemorrhoids. Complications of the patients, such as persistent pain and tenesmus in the MSP group were less than the PPH group. Moreover, MSP had lower costs. Conclusion: MSP is a safe, effective, and inexpensive procedure for patients with hemorrhoidal disease. We consider that this technique should be widely used.
Objetivo: El objetivo del estudio fue comparar la seguridad, los resultados clínicos y el costo entre el procedimiento quirúrgico de suspensión de la mucosa y el manejo quirúrgico del prolapso para el tratamiento de las hemorroides. Métodos: Se incluyeron en el estudio 80 pacientes quienes presentaban hemorroides sintomáticas grado II y III; 40 (50%) recibieron como tratamiento el procedimiento quirúrgico de suspensión de la mucosa y 40 (50%) recibieron el manejo quirúrgico del prolapso. Resultados: Ambos tratamientos quirúrgicos fueron eficientes y seguros observando una menor proporción de persistencia del dolor y tenesmo con el procedimiento quirúrgico de suspensión de la mucosa con costos más bajos para este tipo de cirugía. Conclusión: La suspensión de la mucosa es un procedimiento eficaz, seguro y de bajo costo para los pacientes con enfermedad hemorroidal. Esta técnica debería usarse más ampliamente.