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Objective:To analyse the pathogenic bacteria distribution and clinical characteristics of late-onset sepsis (LOS) among premature infants with gestational age less than 34 weeks in Henan Province.Methods:The clinical data of 6 590 premature infants admitted to 17 medical institutions in Henan Province from January 2019 to December 2020 were retrospectively analyzed. The gestational age of infants was less than 34 weeks and was admitted to the neonatal ward within 7 days after birth. SPSS 19.0 statistical software was used for data analysis.Results:Among 6 590 premature infants LOS developed in 751 cases (11.40%), of whom the diagnosis was confirmed in 276 cases (36.75%) and 475 cases (63.25%) were diagnosed clinically. The fatality rate related to LOS was 13.58%. There were significant differences in the incidence of LOS and infection-related mortality among infants with different gestational ages and body weights ( χ2=388.894 and 13.572, χ2=472.282 and 9.257, P<0.05 or <0.01). Among 276 children with confirmed LOS, 286 strains of pathogenic bacteria were isolated. Gram-negative bacteria were most prevalent (178 strains), accounting for 62.24% of all infections, followed by fungi (58 strains, 20.28%). Klebsiella pneumoniae was most frequently detected Gram-negative bacteria (117 strains, 40.91%), among which 32.48% (38/117) was carbapenem-resistant Klebsiella pneumoniae. The proportion of diagnosed sepsis, the proportion of catheterization, and the infection-related mortality of infants with LOS in tertiary hospitals were all higher than those in secondary hospitals ( χ2=6.212, 5.313 and 4.435, all P<0.05). The proportion of exclusive breastfeeding in secondary hospitals was lower than that in tertiary hospitals ( χ2=19.216, P<0.05). The time of antibacterial drug use before infection in specialized hospitals was longer than that in general hospitals ( χ2=3.276, P<0.05). Conclusion:The incidence of LOS among preterm infants in Henan Province is high, which was mainly caused by Gram-negative bacteria. The clinical characteristics of LOS caused by different pathogens and in different health institutions are different, the prevention and control strategy should be developed accordingly to reduce the incidence LOS of preterm premature infants.
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Objective To investigate the relationship between p53 and miR-29b in myofibroblasts (MFs)induced from human Tenon fibroblasts (HTFs).Methods Human Tenon tissues were obtained from strabismus surgery patients in the First Affiliated Hospital of Anhui Medical University from March to July 2016.The primary HTFs were cultured by tissue culture method and passaged to 2-4 generations.The cells were divided into 4 groups:HTFs group cells were normally cultured,the TGF-β1 activation group cells was activated with 10 ng/ml tumor necrosis factorβ1 (TGF-β1),the siRNA transfection group and the negative control group were transfected with p53 siRNA and negative siRNA,respectively.The expression of p53 protein and cell migration ability of HTFs and MFs were detected by immunohistochemical and cell scratch assay.The relative expression level of miR-29b and p53 mRNA in each group was detected by real-time PCR.This study followed the Helsinki declaration and the patients or their guardians signed informed consent.Results The primary HTFs had long fusiform shape,and were arranged in a spiral shape.After activated by TGF-β1,the cells were transformed into MFs,and the cell morphology was irregular and the arrangement was disordered.The staining intensity of p53 protein in TGF-β1 activation group was significantly stronger than that in HTFs group (t =-10.384,P<0.05).The migration distance of TGF-β1 activation group cells was significantly larger than that of HTFs group (P<0.05).The relative expression level of p53 mRNA was 1.00±0.00,3.95±2.61,0.06±0.06 and 0.98±0.16 in HTFs group,TGF-β1 activation group,siRNA transfection group and negative control group,respectively;the relative expression levels of miR-29b was 1.00±0.00,0.54±0.09,5.10±2.31 and 1.03 ±0.09 in HTFs group,TGF-β31 activation group,siRNA transfection group and negative control group,respectively;the overall differences were statistically significant (p53 mRNA:F =6.688,P =0.006;miR-29b:F=13.640,P=0.000).The relative expression of p53 mRNA was significantly higher and the relative expression of miR-29b was significantly lower in the TGFβ1 activation groups than those in the HTFs group and siRNA transfection group,the differences were statistically significant (all at P<0.05).Conclusions During the process of HTFs transforming to MFs,the expression of p53 is upregulated and the expression of miR-29b is downregulated,Supressing the expression of p53 can upregulate the expression of miR-29b.There may exist interactions between p53 and miR-29b,which regulate the formation of fibrotic scars.
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Objective To study the expressions of miR-10a, miR-93 and miR-200a in malignant ovarian tumor tissues and their clinical significances.Methods Real-time quantitative PCR was used to detect the expression of 40 cases of normal ovarian tissue, 40 cases of benign ovarian cyst and 40 cases of miR-10a, miR-93 and miR-200a in malignant ovarian tumor tissues.Analysis of variance and t-Test were respectively applied to compare the expression of miR in different tissues and analyze the correlation between miR and clinicopathological characteristics of malignant ovarian tumor .Kaplan-Meier Log-rank test and Cox proportional hazards regression model were adopted in prognosis.Results The expression lev-els of miR-10a in ovarian cancer tissues were significantly higher than that in benign ovarian tumor tissues and normal ovari-an tissues (p0.05).The expression quantity of miR-10a in malignant ovarian tumor tissues with greater omen-tum metastasis, lymph node metastasis and distant organ metastasis was significantly higher than that without metastasis ( p<0.05).The median survival time of patients with higher expression of miR -10a was lower than that of patients with low ex-pression of miR-10a (p=0.01).Multi-factor Cox model analysis showed that the expression quantity of miR -10a was an independent factor affecting survival prognosis in patients(p=0.002).Conclusion Our data suggest that miR-10a is asso-ciated with ovarian cancer metastasis, which is the main factor affecting prognosis in ovarian cancer.It might serve as a bio-marker for judging the prognosis in ovarian cancer.
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Objective To evaluate the post-puncture complications in children,and to evaluate the difference in the occurrence rate of headache and backache between patients who had 1 hour of supine recumbency and those who had 4 hours of supine recumbency,to provide evidence for the standardized lumbar puncture (LP) procedure of children.Methods Inpatients who were older than 3 and had diagnostic LP in the course of their treatment between Nov.2012 and Apr.2013 were enrolled.The LP was performed by the same investigator under the standardized institutional guideline for LP and the information of number of LP attempts,duration of LP and cerebrospinal fluid (CSF) drainage volume were recorded.After the procedure,all the children were randomly assigned into 2 groups:the test group had supine recumbency for 1 hour after LP,and the control group had it for 4 hours.For the first 5 days following LP,reports of headache and backache were recorded by another physician who didn't participate in this study.The difference in the occurrence rate of headache and backache between test group and control group was assessed by the chi-square test or Fisher's probabilities in 2 × 2 table.And a Logistic regression analysis was performed to assess the risk factors for the occurrence of the headache and backache.Results The overall frequency of headache was 4.6% (4/87 cases).The frequency of headache was not significantly different between the test group (2.4%) and control group (6.7%) (P =0.617).And the overall frequency of backache was 19.5 % (17/87 cases),and it was not significantly different between the 2 groups (test group:21.4%,control group:17.8 %,P =0.668).In a Logistic regression analysis,age (P =0.011,OR:6.884,95% CI:1.398-33.906) and the previous history of lumbar puncture (P =0.018,0R:0.126,95 % CI:0.026-0.618) were significant risk factors for the occurrence of backache.The risk of backache in children with more than 2 times of LP was decreased.And the risk was higher in the children older than 6 years than those younger than 6 years.Conclusions Headache and backache were the most frequent post-puncture complications in children.There was no difference between short duration (1 hour) of supine recumbency and long duration (4 hours) in preventing the occurrence of headache and backache after LP.