RESUMEN
An increase of biliary lipid secretion is known to occur in the rat under sustained administration of statin-type 3-hydroxy-3-methylglutaryl (HMG) coenzyme A (CoA) reductase inhibitors. The present study has addressed critical mechanisms of hepatic lipid synthesis and phosphatidylcholine (PC) biliary transport in the rat fed with a 0.075% pravastatin diet for 3 weeks. After treatment, biliary secretion of PC and cholesterol increased to 233% and 249% of controls, while that of bile salts was unchanged. Activity of cytidylyltransferase (CT), a major regulatory enzyme in the CDP-choline pathway of PC synthesis, was raised in both microsomal and cytosolic fractions (226% and 150% of controls), and there was an increase to 187% in the mass of active enzyme as determined by Western blot of microsomal protein using an antibody specific to CT. Cytosolic activity of choline kinase, another enzyme of the CDP-choline pathway, also increased to 175% of controls. In addition, there was an over eightfold increase in the HMG CoA reductase activity and mRNA. Thus, an increased PC and cholesterol synthetic supply to hepatocytes appeared as a basic mechanism for the biliary hypersecretion of these lipids. Notwithstanding the increased synthesis, hepatic PC content was unchanged, suggesting an enhanced transfer of this lipid into bile. Indeed, there was a sevenfold increase of multidrug resistance gene 2 (mdr2) gene mRNA coding for a main PC canalicular translocase. Thus, hypersecretion of biliary PC in the model studied can be explained by an up-regulation of mdr2 gene transcription and its P-glycoprotein product mediating the biliary transfer of PC supplied by an increased biosynthesis.
Asunto(s)
Subfamilia B de Transportador de Casetes de Unión a ATP/genética , Transportadoras de Casetes de Unión a ATP/genética , Bilis/metabolismo , Hígado/metabolismo , Fosfatidilcolinas/metabolismo , Pravastatina/farmacología , Transcripción Genética/efectos de los fármacos , Animales , Bilis/efectos de los fármacos , Ácidos y Sales Biliares/metabolismo , Colesterol/metabolismo , Resistencia a Múltiples Medicamentos/genética , Regulación de la Expresión Génica/efectos de los fármacos , Hígado/efectos de los fármacos , Masculino , Ratas , Ratas Sprague-DawleyRESUMEN
To obtain more insight into the mechanisms regulating CTP:phosphocholine cytidylyltransferase (CT), we determined the effect of oleate treatment on the rate of CT release from permeabilized Hep G2 cells and the distribution of the CT remaining in the permeabilized cells. When we permeabilized untreated cells in pH 7.5 buffer containing 0.15 M KCl, the rate of CT release was much slower than the release of lactate dehydrogenase. Oleate treatment caused a further decrease in CT release from cells. In untreated cells, 70-80% of the CT remaining in cells 10 min after permeabilization was recovered as soluble CT. Oleate treatment increased the amount of bound CT but over 50% of the CT in cells 10 min after permeabilization was recovered as soluble CT. In both control and oleate-treated cells, the increase in CT release with time correlated with a decrease in the amount of CT recovered from permeabilized cells as soluble CT. These results suggested that CT existed in a form that was not immediately available for release from permeabilized cells, but was recovered in the soluble fraction after cell disruption. When cells were permeabilized in 10 mM imidazole-20% glycerol-5 mM Mg2+ pH 6.5, over 80% of CT in control and over 90% of CT in oleate-treated cells was recovered bound to the particulate fraction. Essentially no CT was released from the cells. The recovery of CT in the particulate fraction required Mg2+ to be present when permeabilization was initiated. The addition of Mg2+, after cells were disrupted, did not increase CT in the particulate fraction. In untreated cells, 50% of bound CT was active. Oleate treatment increased the amount of active CT in the particulate fraction to over 70% of total. About 50% of particulate CT in untreated cells but only 15% in oleate-treated cells was extracted with 0.15 M KCl. Inactive CT was preferentially extracted by KCl. The bound CT was recovered in isolated nuclei. Overall, the results suggested that both inactive and active CT are bound to nuclear membranes, and that the activation of CT involves conversion of CT loosely bound to membrane to a form more tightly bound to membranes perhaps by hydrophobic interaction with phospholipids. This model does not involve translocation from a soluble pool.
Asunto(s)
Permeabilidad de la Membrana Celular , Citidililtransferasa de Colina-Fosfato/farmacocinética , Proteínas de la Membrana/metabolismo , Transporte Biológico/efectos de los fármacos , Carcinoma Hepatocelular , Permeabilidad de la Membrana Celular/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Humanos , Líquido Intracelular/enzimología , Modelos Biológicos , Membrana Nuclear/enzimología , Ácido Oléico/farmacología , Solubilidad , Células Tumorales CultivadasRESUMEN
We previously identified a protein from rat liver that binds CTP:phosphocholine cytidylyltransferase (CT). We have now purified this protein (cytidylyltransferase-binding protein (CTBP)) from rat liver. The purification involved precipitation at pH 5 and extraction of the precipitate with buffer, followed by sequential chromatography on DEAE-Sepharose and butyl-agarose. Final purification was accomplished by either preparative electrophoresis or hydroxylapatite chromatography. Amino acid sequences from six peptides derived from pure CTBP matched sequences in transcytosis-associated protein (TAP) with 98% identity. Thus, CTBP was positively identified to be TAP. Purified CTBP increased the activity of purified CT measured with phosphatidylcholine (PC)/oleic acid. In the absence of PC/oleic acid, CTBP did not stimulate CT activity. Dilution of CT to reduce the Triton X-100 concentration produced a loss of CT activity. The lost activity was recovered by the addition of CTBP plus PC/oleic acid to the assay, but not by the addition of either PC/oleic acid or CTBP alone. Removal of CTBP from purified preparations by immunoprecipitation with CTBP antibodies eliminated the activation of CT. Both CT and CTBP were shown to bind to PC/oleic acid liposomes. The formation of complexes between CT and CTBP in the absence of PC/oleic acid liposomes could not be demonstrated. These results suggest that CTBP functions to modify the interaction of CT with PC/oleic acid liposomes, resulting in an increase in the catalytic activity perhaps by the formation of a ternary complex between CT, CTBP, and lipid. Overall, these results suggest that CTBP (TAP) may function to coordinate the biosynthesis of phosphatidylcholine with vesicle transport.
Asunto(s)
Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Metabolismo de los Lípidos , Proteínas de la Membrana/química , Proteínas de la Membrana/metabolismo , Nucleotidiltransferasas/metabolismo , Proteínas de Transporte Vesicular , Secuencia de Aminoácidos , Animales , Activación Enzimática , Proteínas de la Matriz de Golgi , Liposomas , Hígado/enzimología , Masculino , Datos de Secuencia Molecular , Unión Proteica , Ratas , Homología de Secuencia de AminoácidoRESUMEN
We previously identified a 112-kDa protein (CTBP) that binds CTP:choline-phosphate cytidylyltransferase (CT) (D.A. Feldman and P.A. Weinhold, 1993, J. Biol. Chem. 268, 3127-3135). In this study we show that fatty acids promote the binding of cytidylyltransferase to CTBP. Gel filtration chromatography separated CTBP from CT in liver cytosol. CTBP was eluted slightly slower than the thyroglobulin standard. The addition of oleate to cytosol followed by incubation at 37 degrees C resulted in the formation of aggregates containing both CT and CTBP. The aggregates eluted in the void volume of the gel filtration column, sedimented to the bottom of glycerol density gradients, and precipitated at concentrations of polyethylene glycol lower than required to precipitate CT and CTBP in untreated cytosol. Immunoprecipitation by CT antibodies of both CT and CTBP in aggregate preparations provided further evidence for the formation of CT-CTBP complexes. A smaller CT-CTBP complex was isolated by glycerol density centrifugation from cytosol incubated with oleate at 4 degrees C. Overall, these results suggest that oleate promotes the binding of CT to CTBP. The results suggest that a dissociable complex is formed at 4 degrees C in the presence of oleate which is polymerized by incubation at 37 degrees C to a large aggregate that is more resistant to dissociation. Complex formation at 37 and 4 degrees C was dose dependent at oleate concentrations between 50 and 200 microM. Complex formation at 4 degrees C was specifically promoted by long-chain, unsaturated fatty acids. These results suggest that CTBP may be involved in the fatty acid-induced translocation of cytidylyltransferase.
Asunto(s)
Proteínas Portadoras/metabolismo , Ácidos Grasos/metabolismo , Nucleotidiltransferasas/metabolismo , Animales , Proteínas Portadoras/aislamiento & purificación , Centrifugación por Gradiente de Densidad , Precipitación Química , Citidililtransferasa de Colina-Fosfato , Cromatografía en Gel , Citosol/efectos de los fármacos , Citosol/metabolismo , Ácidos Grasos/química , Ácidos Grasos/farmacología , Técnicas In Vitro , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Nucleotidiltransferasas/aislamiento & purificación , Polietilenglicoles , Pruebas de Precipitina , Ratas , Ratas Sprague-Dawley , Relación Estructura-ActividadRESUMEN
We studied the effect of choline depletion on the biosynthesis of phosphatidylcholine (PC) and the distribution and phosphorylation of cytidylyltransferase (CT) in HepG2 cells. Phosphocholine concentrations decreased within 24 h of choline depletion to values less than 2% of controls. The incorporation of [3H]glycerol into PC was reduced in choline-depleted (CD) cells. The apparent turnover of PC was similar in CD and choline-supplemented (CS) cells (T1/2 = 20 h). The methylation pathway for PC synthesis increased nearly 10-fold in CD cells. Cell growth was similar in CD and CS cells. Over 95% of CT activity in CS cells was in the soluble pool. Choline depletion resulted in a progressive decrease in CT activity and immunodetected enzyme in the soluble pool and a corresponding increase in membrane CT over a 48-h period. Choline supplementation of CD cells caused a rapid release of membrane CT (complete release by 3 h). Two phosphorylated forms of CT were identified. One form contained a higher level of phosphorylation (HPCT) than the other form (LPCT). HPCT migrated slightly slower than LPCT on SDS gels. CD cells contained only LPCT in both soluble and membrane pools. CS cells contained only HPCT. During choline depletion PC content decreased nearly 20% but CT binding did not occur until LPCT was generated in cytosol. Conversely, choline supplementation released LPCT into cytosol and HPCT was formed only after the release. We conclude that both the induction of binding sites, perhaps by depletion of PC and dephosphorylation of HPCT to LPCT, are required for CT translocation to membranes. The release of CT from membranes is initiated by changes in membrane binding sites followed by trapping of the CT in the soluble pool by phosphorylation of LPCT to HPCT.
Asunto(s)
Colina/metabolismo , Nucleotidiltransferasas/metabolismo , Fosfatidilcolinas/biosíntesis , Sitios de Unión , Transporte Biológico , Membrana Celular/enzimología , Colina/farmacología , Citidililtransferasa de Colina-Fosfato , Humanos , Membranas Intracelulares/enzimología , Fosforilación , Células Tumorales CultivadasRESUMEN
The soluble form of CTP:phosphocholine cytidylyltransferase, which has previously been assumed to be cytosolic, has been localized to the nucleus of several cell types. Indirect immunofluorescence microscopy indicated a nuclear location in HepG2, NIH-3T3, and L-cells. A comparison of the fluorescence pattern of wild-type CHO cells with a cytidylyltransferase-deficient mutant provided genetic evidence that cytidylyltransferase is nuclear in CHO cells. The enzyme is also predominantly nuclear in rat liver, as revealed by staining frozen sections of that tissue. When L-cells were fractionated by enucleation, over 95% of cytidylyltransferase activity was found in the nuclear fraction, providing biochemical evidence for a nuclear location in these cells. In light of the demonstration that the membrane-bound cytidylyltransferase in CHO cells is associated with the nuclear envelope (Watkins, J. D., and Kent, C. (1992) J. Biol. Chem. 267, 5686-5692), these results suggest that this enzyme is predominantly an intranuclear enzyme.
Asunto(s)
Núcleo Celular/enzimología , Proteínas Nucleares/metabolismo , Nucleotidiltransferasas/metabolismo , Células 3T3 , Animales , Western Blotting , Células CHO , Citidililtransferasa de Colina-Fosfato , Cricetinae , Técnica del Anticuerpo Fluorescente , Hígado/enzimología , RatonesRESUMEN
Antisera raised against purified cytidylyltransferase (CT) immunoprecipitated CT activity from liver cytosol and detected the M(r) 45,000 subunit of CT on Western blots. Antisera detected a M(r) 112,000 protein on Western blots of liver cytosol. This protein was not detected in purified CT and was not detected by preimmune serum. The 112-kDa antibodies, isolated by affinity chromatography, did not immunoprecipitate CT activity. Antiserum raised against an N-terminal sequence of CT and antibodies raised against an internal sequence of CT immunoprecipitated CT activity but did not detect the 112-kDa protein. These results showed that the 112-kDa protein was not a form of CT. We concluded that the antiserum probably contained anti-idiotypic antibodies that recognized CT binding sites on the 112-kDa protein. Purified CT that was conjugated to horseradish peroxidase bound to crude 112-kDa protein immobilized on nitrocellulose. The binding was competitively reduced by purified CT and by affinity-purified antibodies to the 112-kDa protein. CT and 112-kDa protein coeluted from DEAE-Sepharose. When the putative 112-kDa protein-CT complex was chromatographed on a second DEAE-Sepharose column or on a Bio-Gel A-1.5m column, CT activity and 112-kDa protein were eluted together. Chromatography of the complex on hydroxylapatite dissociated most of the complex, producing CT free of the 112-kDa protein. We conclude that the 112-kDa protein is a CT-binding protein. The formation and/or dissociation of this complex may be important in the regulation of CT.
Asunto(s)
Hígado/enzimología , Nucleotidiltransferasas/metabolismo , Proteínas/metabolismo , Animales , Complejo Antígeno-Anticuerpo , Unión Competitiva , Western Blotting , Citidililtransferasa de Colina-Fosfato , Cromatografía de Afinidad , Cromatografía por Intercambio Iónico , Electroforesis en Gel de Poliacrilamida , Sueros Inmunes , Sustancias Macromoleculares , Peso Molecular , Nucleotidiltransferasas/inmunología , Nucleotidiltransferasas/aislamiento & purificación , Unión Proteica , Proteínas/inmunología , Proteínas/aislamiento & purificación , RatasAsunto(s)
Citosol/enzimología , Hígado/enzimología , Nucleotidiltransferasas/análisis , Animales , Citidililtransferasa de Colina-Fosfato , Estabilidad de Enzimas , Cinética , Lípidos/farmacología , Liposomas , Peso Molecular , Nucleotidiltransferasas/antagonistas & inhibidores , Nucleotidiltransferasas/efectos de los fármacos , RatasRESUMEN
Fatty acids are known to cause an increase in the incorporation of radioactive choline into phosphatidylcholine. A coincident increase in membrane cytidylyltransferase activity is well documented. The purpose of the present studies was to determine the direct effects of oleic acid on the kinetic properties of membrane cytidylyltransferase. An examination of the reaction characteristics of membrane cytidylyltransferase revealed that membranes from adult rat lung contained high CTPase activity. This activity prevented the determination of reaction velocities at low CTP concentrations. The CTPase activity was blocked by the addition of ADP or ATP to the reaction. The addition of 6.0 mM ADP to the assay mixture enabled us to determine the effect of oleate on the CTP Km. Oleate (122 microM) caused a significant decrease in CTP Km for microsomal cytidylyltransferase (0.99 mM to 0.33 mM) and H-Form cytidylyltransferase (1.04 mM to 0.27 mM). Oleate did not decrease the CTP Km for L-Form cytidylyltransferase. Oleate had no effect on the choline phosphate Km in microsomal, H-Form or L-Form cytidylyltransferase. Oleate also increased the Vmax for cytidylyltransferase. The increase was dependent upon the concentration of oleate with a maximal increase of 50-60% at 100-130 microM oleate. We conclude that oleate has a direct stimulatory effect on cytidylyltransferase when it is in the active form (membrane bound or H-Form lipoprotein complex). We suggest that the kinetic effects operate synergistically with other regulatory mechanisms such as translocation or conversion of inactive to active species. The direct effect of oleate on the cytidylyltransferase may be an important regulatory mechanism when CTP concentrations are limiting.
Asunto(s)
Ácidos Grasos/farmacología , Microsomas/enzimología , Nucleotidiltransferasas/efectos de los fármacos , Animales , Centrifugación por Gradiente de Densidad , Citidililtransferasa de Colina-Fosfato , Técnicas In Vitro , Cinética , Pulmón/enzimología , Masculino , Microsomas Hepáticos/enzimología , Nucleotidiltransferasas/metabolismo , Ácido Oléico , Ácidos Oléicos/farmacología , RatasRESUMEN
Lipids, particularly surface-active phospholipids, have been proposed to provide an important protective barrier in the gastric mucosa. The predominant surface-active phospholipid in the pulmonary surfactant complex is dipalmitoylphosphatidylcholine. To determine whether the gastric epithelium synthesizes and secretes this phospholipid, primary cultures of canine gastric mucous cells isolated by counterflow elutriation were studied. During the 24-hour period of culture, the gastric mucous cells incorporated 3H-choline into phosphatidylcholine, with dipalmitoylphosphatidylcholine representing 13.8% +/- 0.6% of the phosphatidylcholine synthesized. When mucous cell preparations with greater chief cell contamination were studied, they incorporated significantly less precursor into dipalmitoylphosphatidylcholine. Administration of prostaglandin E2, a cytoprotective agent, to the cultured mucous cells for 1 hour led to a significant increase in phosphatidylcholine release, reaching a maximum of 120.4% +/- 4.2% (P less than 0.001) at 10(-6) mol/L. No significant stimulation of phospholipid release by prostaglandin E2 was seen in the fractions containing a greater proportion of chief cells. To further establish the relationship between mucin and phospholipid secretion, two gastric cancer cell lines, Hs746T and KATO III, were studied. Using immunocytochemical and biochemical techniques, mucin synthesis and secretion were confirmed by these cell lines. The Hs746T cells were significantly more active in the secretion of both mucin and phospholipid than the KATO III cells. The Hs746T line secreted 5.7-fold more mucin and 7.3-fold more phospholipid than KATO III cells during a 24-hour period of culture. The association between mucin and phospholipids in an aqueous solution was also studied. Purified mucin in the concentration of 0.5-2 mg/mL of glycoprotein led to a significant dose-dependent increase in phospholipid solubility, suggesting the formation of a glycoprotein-phospholipid complex. The current studies indicate that the gastric mucous cell is the source of surfactant phospholipids as well as mucin. The synthesis and release of mucin and phospholipid are functions of the mucous cell that play a critical role in the primary defense of gastric epithelium.
Asunto(s)
1,2-Dipalmitoilfosfatidilcolina/biosíntesis , Dinoprostona/farmacología , Mucinas Gástricas/metabolismo , Mucosa Gástrica/metabolismo , Fosfolípidos/metabolismo , 1,2-Dipalmitoilfosfatidilcolina/metabolismo , Animales , Células Cultivadas , Perros , Epitelio/metabolismo , Mucinas Gástricas/biosíntesis , Mucinas Gástricas/química , Mucosa Gástrica/efectos de los fármacos , Fosfatidilcolinas/metabolismo , Células Tumorales CultivadasRESUMEN
We examined the effect of fatty acids on phosphatidylcholine synthesis and cytidylyltransferase activity in Hep G2 cells. Treatment of Hep G2 cells with oleic acid caused an increase in the incorporation of [methyl-14C]choline into phosphatidylcholine and a corresponding decrease in radioactivity in choline phosphate using a pulse-chase procedure. This result is consistent with a fatty acid-induced increase in the cytidylyl-transferase step in the choline pathway. We measured cytidylyltransferase activity in membrane fractions and in cytosol (100,000 x g supernatant or soluble enzyme released by digitonin). The activity increased in both membrane and cytosol. Thus, an increase in total activity occurred. Cytidylyltransferase protein determined by Western blot immunoassay increased after oleic acid treatment. Immunotitration of cytidylyltransferase protein also indicated that an increase in enzyme protein resulted from oleic acid treatment. Cycloheximide did not prevent the oleic acid-induced increase in cytidylyltransferase activity. The increase in enzyme activity was apparent when we measured the activity in the presence or absence of lipid activators. Separation of cytosolic cytidylyltransferase into H- and L-forms showed that the increase in cytosolic activity was due to an increase in H-form. The amount of L-form did not change. We interpret these results to suggest that fatty acid treatment of Hep G2 cells promoted the formation of active cytidylyltransferase (H-form) from a preexisting inactive form. The increased activity was distributed between membranes and the lipoprotein form in cytosol (H-form).
Asunto(s)
Carcinoma Hepatocelular/metabolismo , Neoplasias Hepáticas/metabolismo , Nucleotidiltransferasas/metabolismo , Fosfatidilcolinas/biosíntesis , Western Blotting , Citidililtransferasa de Colina-Fosfato , Cicloheximida/farmacología , Digitonina/farmacología , Ácido Oléico , Ácidos Oléicos/farmacología , Células Tumorales Cultivadas/efectos de los fármacosRESUMEN
The activity of choline-phosphate cytidylyltransferase is increased by glucocorticoids in late gestation fetal lung in association with increased phosphatidylcholine biosynthesis. Previous indirect data had suggested that the stimulatory effect of the hormone was due to activation of existing enzyme rather than synthesis of new cytidylyltransferase protein. Using a rabbit antibody raised against purified rat liver choline-phosphate cytidylyltransferase, we have now quantitated the amount of the enzyme in fetal rat lung explants cultured with and without dexamethasone. Our results show that the hormone increased the activity of the enzyme but not the amount of cytidylyltransferase protein. Thus the stimulatory effect of dexamethasone on cytidylyltransferase is due to activation of existing enzyme rather than induction of enzyme synthesis.
Asunto(s)
Dexametasona/farmacología , Pulmón/embriología , Nucleotidiltransferasas/metabolismo , Animales , Western Blotting , Citidililtransferasa de Colina-Fosfato , Activación Enzimática/efectos de los fármacos , Edad Gestacional , Técnicas de Inmunoadsorción , Pulmón/efectos de los fármacos , Pulmón/enzimología , Fosfatidilcolinas/biosíntesis , Ratas , Ratas EndogámicasRESUMEN
The purpose of these studies was to determine the properties of the membrane-bound cytidylyltransferase in adult lung and to assess the relationship between the microsomal enzyme and the two forms of cytidylyltransferase in cytosol. Microsomes, isolated by glycerol density centrifugation, contained significantly less cytidylyltransferase than microsomes isolated by differential centrifugation (11.6 +/- 3.2 vs. 30 +/- 11 nmol/min per g lung). The released activity was recovered as H-form cytidylyltransferase. Cytidylyltransferase activity was not removed from microsomes by washing of the microsomal pellet with homogenizing buffer. Triton X 100 extracted all of the cytidylyltransferase from microsomes. The extracted activity was similar to H-form. Chlorpromazine dissociated microsomal enzyme to L-form. Chlorpromazine has been shown previously to dissociate H-form to L-form. These results suggested that microsomal cytidylyltransferase existed in a form similar if not identical to cytosolic H-form. In vitro translocation experiments demonstrated that the L-form of cytidylyltransferase was the species which binds to microsomal membranes. Triton X 100 extraction of microsomes from translocations experiments removed the bound enzyme activity. Glycerol density fractionation indicated that the activity in the Triton extract was H-form cytidylyltransferase. We concluded that the active lipoprotein form of cytidylyltransferase (H-form) is the membrane-associated form of cytidylyltransferase in adult lung; that it is formed after the L-form binds to microsomal membranes and that cytosolic H-form is released from the membrane.
Asunto(s)
Isoenzimas/metabolismo , Pulmón/enzimología , Microsomas/enzimología , Nucleotidiltransferasas/metabolismo , Animales , Fraccionamiento Celular , Centrifugación por Gradiente de Densidad , Citidililtransferasa de Colina-Fosfato , Citosol/enzimología , Diacilglicerol Colinafosfotransferasa/aislamiento & purificación , Diacilglicerol Colinafosfotransferasa/metabolismo , Electroforesis en Gel de Poliacrilamida , Feto , Membranas Intracelulares/enzimología , Isoenzimas/aislamiento & purificación , Masculino , Peso Molecular , Nucleotidiltransferasas/aislamiento & purificación , Ratas , Ratas EndogámicasRESUMEN
The subcellular forms of cytidylyltransferase (EC 2.7.7.15) in rat lung, rat liver, Hep G2 cells, A549 cells and alveolar Type II cells from adult rats were separated by glycerol density centrifugation. Cytosol prepared from lung, Hep G2 cells, A549 cells and alveolar Type II cells contained two forms of the enzyme. These species were identical to the L-Form and H-Form isolated previously from lung cytosol by gel filtration. Liver cytosol contained only the L-Form. Rapid treatment of Hep G2 cells with digitonin released all of the cytoplasmic cytidylyltransferase activity. The released activity was present in both H-Form and L-Form. The molecular weight of L-Form was determined from sedimentation coefficients and Stokes radius values to be 97,690 +/- 10,175. Thus, the L-Form appears to be a dimer of the Mr 45,000 catalytic subunit. The f/f degrees value of 1.5 indicated that the protein molecule has an axial ratio of 10, assuming a prolate ellipsoid shape. The estimated molecular weight of the H-Form was 284,000 +/- 25,000. The H-Form was dissociated into L-Form by incubation of cytosol at 37 degrees C. Triton X-100 (0.1%) and chlorpromazine (1.0 mM) also dissociated the H-Form into L-Form. Western blot analysis indicated that both forms contained the catalytic subunit. An increase in Mr 45,000 subunit coincided with the increase in cytidylyltransferase activity in L-Form, which resulted from the dissociated of H-Form. The L-Form was dependent on phospholipid for activity. The H-Form was active without lipid. Phosphatidylinositol was present in the H-Form isolated from Hep G2 cells. The phosphatidylinositol dispersed when the H-Form was dissociated into L-Form. Phosphatidylinositol and phosphatidylglycerol cause L-Form to aggregate into a form similar to H-Form. Phosphatidylcholine/oleic acid (1:1 molar ratio) and oleic acid also aggregated the L-Form. Phosphatidylcholine did not produce aggregation. We conclude that the H-Form is the active form of cytidylyltransferase in cytoplasm. The H-Form appears to be a lipoprotein consisting of an apoprotein (L-Form dimer of the Mr 45,000 subunit) complexed with lipids. A change in the relative distribution of H-Form and L-Form in cytosol would alter the cellular activity and thus may be important in the regulation of phosphatidylcholine synthesis.
Asunto(s)
Carcinoma Hepatocelular/enzimología , Neoplasias Hepáticas/enzimología , Neoplasias Pulmonares/enzimología , Pulmón/enzimología , Nucleotidiltransferasas/aislamiento & purificación , Alveolos Pulmonares/enzimología , Adenoma/enzimología , Animales , Centrifugación por Gradiente de Densidad , Fenómenos Químicos , Química Física , Citidililtransferasa de Colina-Fosfato , Citosol/enzimología , Digitonina , Humanos , Hígado/enzimología , Masculino , Peso Molecular , Nucleotidiltransferasas/metabolismo , Ratas , Ratas Endogámicas , Células Tumorales CultivadasRESUMEN
The initiation of pulmonary surfactant synthesis during fetal development has been shown to be under hormonal control. Using cultured lung cells isolated from 19-day-gestation fetal rats, we evaluated the effects of various hormones on the activity and subcellular distribution of cholinephosphate cytidylyltransferase, a rate-controlling enzyme in phosphatidylcholine synthesis. The cells were incubated in medium containing 10% carbon-stripped fetal bovine serum to which dexamethasone, triiodothyronine, and/or conditioned medium from dexamethasone-treated fetal rat lung fibroblasts were added for 48 h. Dexamethasone and fibroblast-conditioned medium increased microsomal enzyme activity 169% +/- 6% (mean +/- SE, p less than 0.01) and 150% +/- 2% (p less than 0.05) over control levels, respectively. Further, dexamethasone increased cytosolic specific activity 160% +/- 17% (p less than 0.05). Addition of T3 to the fibroblast-conditioned medium caused a further increase in microsomal activity, but T3 alone had no effect. Increased microsomal cytidylyltransferase activity correlated with an increased rate of [3H]choline incorporation into disaturated phosphatidylcholine. Hormonal induced increases in enzyme activity were not adequately explained by simple translocation of enzyme from cytosol to microsomes. Cycloheximide (5 micrograms/ml) inhibited enzyme stimulation by dexamethasone and fibroblast-conditioned medium, suggesting that protein synthesis of new enzyme or regulatory proteins is involved. We conclude that hormones modulate cytidylyltransferase activity of isolated fetal lung cells. Dexamethasone and fibroblast-conditioned medium exert their major effects by stimulating microsomal activity.
Asunto(s)
Dexametasona/farmacología , Pulmón/enzimología , Nucleotidiltransferasas/análisis , Triyodotironina/farmacología , Animales , Células Cultivadas , Citidililtransferasa de Colina-Fosfato , Medios de Cultivo/farmacología , Cicloheximida/farmacología , Feto/citología , Fibroblastos/efectos de los fármacos , Cinética , Pulmón/citología , Pulmón/embriología , Fosfatidilcolinas/análisis , Ratas , Fracciones Subcelulares/enzimologíaRESUMEN
Ethanolaminephosphotransferase (EC 2.7.8.1) activity was determined in lung microsomes using diacylglycerols generated endogenously from [14C]glycerol 3-phosphate and different mixtures of fatty acids. Ethanolaminephosphotransferase used endogenously generated dipalmitoylglycerol better than dioleoylglycerol. The apparent Km and the reaction rates for four different endogenously generated mixtures were the same (16 nmol/mg microsomal proteins). The apparent Km values for CDP-ethanolamine were the same (0.26 mm) for endogenously generated dipalmitoylglycerol and dioleoylglycerol. The amount of diacylglycerol generated in microsomes was 2-3-times the apparent Km for diacylglycerol. Dipalmitoylglycerol, supplied exogenously as a Tween 20/phosphatidylglycerol emulsion, was nearly twice as active as dioleoylglycerol. Both dipalmitoylglycerol and dioleoylglycerol were more active as substrates when emulsions were made with phosphatidylglycerol/Tween 20 than with Tween 20 alone. The results suggest that ethanolaminephosphotransferase in lung is relatively nonselective for molecular species of diacylglycerol. In addition, the results suggest that the concentration of diacylglycerol and the physical state in which it is presented to the enzyme can affect the apparent selectivity of ethanolaminephosphotransferase for diacylglycerols.
Asunto(s)
Diglicéridos/metabolismo , Etanolaminofosfotransferasa/metabolismo , Glicéridos/metabolismo , Pulmón/enzimología , Fosfotransferasas/metabolismo , Animales , Relación Dosis-Respuesta a Droga , Ácidos Grasos/metabolismo , Masculino , Microsomas/enzimología , Fosfatidiletanolaminas/biosíntesis , Ratas , Ratas Endogámicas , Especificidad por SustratoRESUMEN
We reported previously the purification of CTP:phosphorylcholine cytidylyltransferase from rat liver (Weinhold, P. A., Rounsifer, M. E., and Feldman, D. A. (1986) J. Biol. Chem. 261, 5104-5110). The purified enzyme appeared to contain equal amounts of two nonidentical proteins, with Mr of about 38,000 and 45,000. We have now separated and purified these proteins. Polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate indicated that each protein was homogeneous. The 45,000 protein contained the catalytic activity. Analysis by gel filtration chromatography and glycerol gradient centrifugation indicated that the 38,000 and 45,000 proteins in the purified cytidylyltransferase were independently associated with Triton X-100 micelles. The apparent Mr of the complexes suggested that a tetramer of each protein was bound to one Triton X-100 micelle. The isolated 45,000 catalytic protein had the same lipid requirement and kinetic properties as the purified cytidylyltransferase containing both proteins. Enzyme activity was stimulated to maximal values by phosphatidylcholine vesicles containing 9 mol % of either oleic acid, phosphatidylinositol, or phosphatidylglycerol. The amino acid compositions of the isolated 38,000 and 45,000 proteins were distinctly different. Overall, the results suggested that a tetramer of the 45,000 protein possessed nearly optimal catalytic activity. A functional role of the 38,000 protein as part of a cytidylyltransferase enzyme complex could not be documented. However, the need for stabilizing concentrations of Triton X-100 in the purified enzyme preparation may have prevented the association of the two proteins.
Asunto(s)
Hígado/enzimología , Nucleotidiltransferasas/metabolismo , Aminoácidos/análisis , Citidililtransferasa de Colina-Fosfato , Electroforesis en Gel de Poliacrilamida , Cinética , Lípidos/análisis , Espectroscopía de Resonancia Magnética , Peso Molecular , Octoxinol , Ácido Oléico , Ácidos Oléicos/farmacología , Fosfatidilcolinas/farmacología , Fosfatidilgliceroles/farmacología , Polietilenglicoles/farmacologíaRESUMEN
We have purified CTP:phosphorylcholine cytidylyltransferase from rat liver cytosol 2180-fold to a specific activity of 12,250 nmol/min/mg of protein. The purified enzyme was stable at -70 degrees C in the presence of Triton X-100 and 0.2 M phosphate. The purified enzyme gave a single protein and activity band on nondenaturing polyacrylamide electrophoresis. Separation by sodium dodecyl sulfate-polyacrylamide electrophoresis indicated that the purified enzyme contained subunits with Mr of 39,000 and 48,000. Gel filtration analysis indicated that the native enzyme was a tetramer containing two 39,000 and two 48,000 subunits. The purified enzyme appeared to bind to Triton X-100 micelles, one molecule of tetramer/micelle. Maximal activity was obtained with 100 microM phosphatidylcholine-oleic acid vesicles (8-10-fold stimulation). Phosphatidylglycerol produced a 4-5-fold increase in activity at 10 microM. The pH optimum and true Km values for CTP and phosphorylcholine were similar to those reported previously for crude preparations of cytidylyltransferase. The overall behavior of cytidylyltransferase during purification and subsequent analysis suggested that it has hydrophobic properties similar to those exhibited by membrane proteins.
Asunto(s)
Hígado/enzimología , Nucleotidiltransferasas/metabolismo , Animales , Precipitación Química , Citidililtransferasa de Colina-Fosfato , Cromatografía , Citosol/enzimología , Estabilidad de Medicamentos , Electroforesis en Gel de Poliacrilamida , Femenino , Sustancias Macromoleculares , Micelas , Peso Molecular , Nucleotidiltransferasas/antagonistas & inhibidores , Nucleotidiltransferasas/aislamiento & purificación , Octoxinol , Fosfolípidos/farmacología , Polietilenglicoles/farmacología , Ratas , Reactivos de Sulfhidrilo/farmacologíaRESUMEN
Triacylglycerol lipase with maximal activity at pH 5 was present in adult and fetal lung. The activity was inhibited by serum concentrations used to measure lipoprotein lipase and by 0.5 M NaCl. The activity in homogenates from fetal lung was about 40% of the activity in adult lung homogenates. The activity increased to 80% of the adult levels during the first 24-48 h following birth. Acidic triacylglycerol lipase was present in all subcellular fractions from adult lung. However, the major amount of activity appeared to be associated with lysosomes. Fetal lung contained significantly more activity in the cytosolic fraction compared to the adult. The reaction produced free fatty acids (65%), 1,2(2,3)-diacylglycerol (22%) and 2-monoacylglycerol (12%). Minimal amounts of 1,3-diacylglycerol and 1(3)-monoacylglycerol were formed. Diacylglycerol lipase and monoacylglycerol hydrolase activities at pH 5 were independently determined and both were higher than the triacylglycerol lipase activity. The subcellular distribution of diacylglycerol lipase and monoacylglycerol hydrolase differed from that of triacylglycerol lipase. Overall, the results indicated that the lung has considerable intracellular lipase activity and therefore could readily hydrolyze intracellular triacylglycerol to free fatty acids. The reaction also produced significant amounts of 1,2-diacylglycerol which suggests that triacylglycerol could be a direct source of diacylglycerol for phospholipid synthesis.
Asunto(s)
Lipasa/metabolismo , Pulmón/enzimología , Envejecimiento , Animales , Fraccionamiento Celular , Femenino , Feto , Concentración de Iones de Hidrógeno , Cinética , Lipasa/aislamiento & purificación , Pulmón/embriología , Pulmón/crecimiento & desarrollo , Embarazo , Ratas , Fracciones Subcelulares/enzimologíaRESUMEN
The activity of the low molecular weight form of cytidylyltransferase from fetal lung cytosol and adult liver cytosol was stimulated more by phosphatidylcholine-oleic acid (1:1 molar ratio) vesicles than by phosphatidylglycerol vesicles. Phosphatidylcholine alone did not stimulate the activity, while oleic acid alone produced only slight stimulation. Vesicles prepared from phosphatidylinositol, phosphatidylglycerol-cholesterol (2:1) and phosphatidylglycerol-phosphatidylcholine (1:1) all stimulated the activity to the same extent. Phosphatidylcholine-oleic acid vesicles (molar ratio 2:1) produced less stimulation than 1:1 vesicles. Phosphatidylcholine-palmitic acid vesicles (2:1) were about 50% as active as the corresponding phosphatidylcholine-oleic acid vesicles. All vesicles were in the size range of small unilamellar vesicles as judged by Sephacryl S-1000 chromatography. Stimulation also occurred when phosphatidylcholine vesicles and oleic acid were added separately to the assay. The stimulation by phospholipid vesicles was correlated with the ability of the vesicles to bind cytidylyltransferase, determined by sucrose density centrifugation of the enzyme-vesicles mixtures. We conclude that the stimulation of soluble cytidylyltransferase occurs through binding of the enzyme to anionic membrane surfaces. Suitable anionic membranes can be prepared either from anionic phospholipids, or by the addition of anionic lipids (unesterified fatty acids or phosphatidylglycerol) to phosphatidylcholine.