Treatment and biomechanical assessment of patients with chronic sacroiliac joint syndrome.

OBJECTIVE: To evaluate diagnostic and biomechanical correlates and treatment outcomes of manipulative/adjustive care in patients highly selected for sacroiliac joint syndrome (SIJS). DESIGN: Descriptive case series, 1 wk baseline, 1 yr follow-up. SETTING: Private chiropractic practice. PATIENTS: Ten out of 153 consecutive new patients (4 male and 6 female) with "primary," chronic, uncomplicated SIJS were selected over an 11-mo period on the basis of painful SIJ and provocation tests. MAIN OUTCOME MEASURES: Back pain (visual analogue scale), Oswestry disability index, lumbar provocation tests and biomechanical measures of gait and postural sway. INTERVENTION: Six-wk regimen of mechanical force, manually assisted, short lever adjustments (MFMA) with an Activator instrument. RESULTS: Pain decreased significantly from a mean baseline value of 25 to 12 (t = 2.28; p < .05). Likewise, the average disability scores diminished from 28 to 13% (t = 2.3; p < .05), and a reduction in the number of positive provocation tests was noted (Fisher Exact Probability range Z = 0.025-0.045). Gait and sway parameters were indistinguishable from normals, before or after treatment. Response to the 1-yr follow-up questionnaire (6/10) revealed stability of symptoms at a low level. CONCLUSIONS: While the majority of subjects recorded some degree of positive outcome, we conclude that: a) discrete SIJS remains difficult to diagnose, but may be possible by judicious choice of screening tests; b) MFMA may benefit some patients with chronic SIJ pain; and c) gait and sway measurement yielded no correlation with clinical conditions.

Proliferation of cultivated cells from normal, preneoplastic and neoplastic human breast tissue--preliminary report.

The success of cultivation of epithelial cells from normal, neoplastic and preneoplastic tissues is reviewed. MCDB 170 medium with special supplements is useful for epithelial cell culture. The epithelial character was demonstrated by the reaction with 3 different monoclonal antibodies. Most cell populations from preneoplastic and neoplastic tissues were identified as fibroblasts. Cell proliferation as measured by cell doubling time, labeling index, flow cytometry did not differ between the tissue types examined. Only 3H-thymidine uptake showed an increased tendency in cells from preneoplastic and neoplastic tissues. The use of normal human breast epithelial cells for molecular biological studies is recommended.

Response of different mammary epithelial cell lines to a mammary derived growth inhibitor (MDGI).

MDGI, a 14.5-KDa protein, is a chemically defined growth inhibitor, which we have purified from lactating bovine mammary gland and characterized biologically in a mouse Ehrlich ascites mammary tumour (EAT) short term suspension culture. It has now been tested for its inhibitory activity on proliferation of four malignant mammary epithelial cell lines of human and mouse origin and normal human mammary epithelial cells. In all experiments, cells were brought to quiescence by serum or growth factor deprivation. Using [3H]TdR pulse labelling the effect of MDGI was measured on the restimulation of proliferation after medium change. MaTu and T47 D, human malignant mammary epithelial cell lines, as well as the mouse malignant mammary epithelial cell line mMaCa 20177 could be inhibited, whereas the human malignant mammary epithelial cell line MCF7 showed a slight stimulation. MDGI showed no activity on the residual DNA synthesis of all cell lines after starvation. Normal human mammary epithelial cells (HMEC) of different passages could also be inhibited. Their responsiveness seemed to be dependent on the number of passages. Cells from high passages (10-14) showed a higher sensitivity, which is also about 10 times higher than that of the malignant cell lines. Furthermore, growth factors like insulin, epidermal growth factor (EGF) and fetal calf serum (FCS), known to be potent antagonists to the MDGI activity in the EAT and, in the case of insulin, also in the MaTu culture (shown in the present study), do not abolish the inhibitory activity of MDGI on HMEC cells. These results demonstrate that the inhibitory activity of MDGI is not exclusively restricted to EAT cells studied so far.

Secretion of phosphoproteins associated with neoplastic transformation and with the action of transforming growth factors.

Incubation of conditioned media derived from mammary epithelial cells and from fibroblasts with [gamma-32P]ATP revealed much higher intensities of labeled polypeptides in transformed cells compared to normal cells. Conditioned media from human mammary carcinoma cells have in common the presence of characteristic phosphoproteins with molecular masses of about 37,22 and 19.5 kDa. Ehrlich Ascites Mammary Carcinoma Cells secrete a dominant 32 kDa phosphoprotein. Normal fibroblasts secrete elevated levels of phosphoproteins after treatment with transforming growth factors. A phosphoprotein with molecular mass of about 37 kDa becomes secreted preferentially if cell-conditioned media were labeled in vivo. The results indicate that the phosphoproteins comprise a family of secretory polypeptides associated with early steps of transformation.

Monoclonal antibodies against antigens of the human mammary carcinoma cell line MCF-7.

Mouse monoclonal antibodies against the human mammary carcinoma cell line, MCF-7, were produced and tested against a panel of cell lines. They recognize a variety of intra- and extracellular antigens. Four of them did not react with the human rhabdomyosarcoma cell line A-204 used as a discriminating second target during initial screening. One of these, 45-B/B3, is specific for epithelia, and probably recognizes a cytokeratin. This antibody clearly differentiates carcinomas from sarcomas and lymphomas, and epithelial cells in culture from fibroblasts. It is not species-restricted.

[Isolation of foamy virus type II out of haematopoetic cells from baboons with haematoblastoses and from healthy animals (author's transl)]. / Isolierung des Foamy-Virus Typ II aus den Zellen der blutbildenden Organe von gesunden und an Hämoblastose erkrankten Mantelpavianen.

Foamy virus Type II persists in the haematopoetic organs of 75 percent of baboons in the Suchumi flock. A mixed infection with foamy virus types I and II seems to be possible. Foamy viruses are isolated as well from monkeys with haemoblastoses as from healthy animals. New information concerning the intrauterine transmission of foamy viruses were obtained.

[Investigation of electrophoretical enzyme phänotypes glucose-6-phosphate dehydrogenase, red cell acid phosphatase, phosphoglucomutase and adenosine deaminase of permanent human cell lines (author's transl)]. / Untersuchung der elektrophoretischen Enzymphänotypen Glukose-6-Phosphatdehydrogenase, Saure Erythrozytenphosphatase, Phosphoglukomutase und Adenosindesaminase von 11 permanenten Menschzellinien

Isoenzymes of 11 cell lines were investigated by electrophoretical separation. All lines have been cultivated from tissues of white persons, all but one (Leuc. Th. B.) were phosphoglucomutase 1 and all were adenosine deaminase 1. Three out of 11 cell lines did show glucose-6-phosphate dehydrogenase (G6PD) type B as expected. Two out of 8 cell lines with G6PD A were distinguishable by their specific type of "red cell" acid phosphatase (SEP). We conclude that in vitro the electrophoretical G6PD phänotyp B changed to phänotype A. Further 4 lines had other peculiarities which are indicative to their originality, though they were G6PD A. Our investigations did show that G6PD may become type A if a cell line changes to permanent growth capacity in vitro. The enzyme marker G6PD A alone may not be valuated as an absolute evidence for contamination or mix up with He-La Cells.

[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)]. / Eliminierung eines Paramyxovirus aus Oncornavirus-produzierenden menschlichen Zellinien

A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.

[Presence of RNA-and DNA-tumor viruses within a cell line from mouse spleen and thymus (author's transl)]. / Gleichzeitiges Vorkommen von RNS- und DNS-Tumorviren in einer Milz-Thymus-Zellinie der Maus

Cells of spleen and thymus from mice infected with Rauscher leukaemia virus were cultivated in vitro. A new cell type repeatedly grew out of this cell line. Electron-optically, RNA--viruses and DNA-viruses were found within the new lines, but there was no cell containing both viruses at the same time. According to experimental results in rats, the DNA virus is polyoma-like. The importance the presence of two different viruses within the same cell is discussed.

[C-type-like particles in normal human placentas (author's transl)]. / C-Typ-ähnliche Partikel in normalen menschlichen Plazenten

Evidence is presented for the presence of C-type-like particles in the syncytiotrophoblast of normal human placentas. In 10 of 110 mainly immature placentas studied both extracellular and budding particles were found.

Serological analysis of an oncornavirus (PMF virus) detected in malignant permanent human cell lines.

Immunodiffusion analysis of the PMF virus which was detected in malignant permanent human cell lines revealed positive reactions with antisera against the Mason-Pfizer monkey virus (MPMV). No cross-reactivity was demonstrated with murine leukemia virus (MuLV), rat leukemia virus (RaLV), hamster leukemia virus (HaLV), feline leukemia virus (FeLV), simian (woolly monkey) sarcoma virus (SSV-1) and mouse mammary tumor virus (MTV). The cross-reactive antigens of the PMF virus and the MPMV are considered as evidence for the human origin of the PMF virus.