Impacts of heat stress on the accuracy of a noseband sensor for detection of eating and rumination behavior in confined cattle.

Precision monitoring of feeding behaviors can aid in dairy herd management. Noseband sensors (RumiWatch System [RW]; Itin + Hoch GmbH) have been established as an automated gold standard for evaluating precision technologies in grazing cows, but more advanced algorithms have not been validated in confinement settings. Additionally, little is known regarding effects of environmental conditions on sensor performance. Therefore, accuracy of RW in quantifying eating and rumination time in confinement was evaluated using 2 versions of the analysis software algorithms (RW Converter V.7.3.2 and V.7.3.36) under thermoneutral (TN; 21.0°C, 64.0% relative humidity [RH], temperature-humidity index [THI] = 67) and heat stress conditions (HS; cyclical daily temperatures to mimic diurnal patterns; 0700-1900 h: 33.6°C, 40.0% RH, THI = 83.5; 1900-0700 h: 23.2°C, 70.0% RH; THI = 70.3). Nine individually housed Holstein × Simmental cross steers were fitted with RW noseband sensors. Agreement for eating time reported by RW and visual observations (1-min scan sampling) was very high in TN regardless of software version (concordance correlation coefficient [CCC]: V.7.3.2 = 0.91; V.7.3.36 = 0.94), and remained high to very high (CCC: V.7.3.2 = 0.89; V.7.3.36 = 0.95) during HS. Agreement for rumination time was very high regardless of software version in both TN (CCC: V.7.3.2 = 0.93; V.7.3.36 = 0.99) and HS (CCC: V.7.3.2 = 0.91; V.7.3.36 = 0.99). Overall, RW accurately quantified eating and ruminating time in confined cattle, and noseband sensors retained accuracy during heat stress. These results indicate RW may serve as a benchmark for future precision technology validations in dairy cattle managed in confinement systems.

Impacts of heat stress on the accuracy of an ear-tag accelerometer for monitoring rumination and eating behavior in dairy-beef cross cattle using an automated gold standard.

Accelerometer-based technologies can be utilized for precision monitoring of feeding behaviors, but limited information is available regarding the impact of varying environmental conditions on sensor performance. The objective of this study was to determine if a commercially available ear-tag sensor (CM; CowManager SensOor, Agis Automatisering BV) could accurately quantify eating and rumination time under heat stress conditions. Data obtained from CM sensors was compared with data collected using an automated gold standard (RW; Rumiwatch System; Itin+Hoch). Automated measurements were obtained from 2 experiments in which cattle were exposed to heat stress conditions. In the principal study (Experiment 1), 3428 h of data were collected from 9 Holstein × Angus steers (470.9 ± 23.9 kg) subjected to either thermoneutral (TN; 21.0°C; 64.0% humidity; temperature-humidity index [THI] = 67; 12- and 12-h light and dark cycle; n = 1714 h), or heat stress conditions (HS; cyclical daily temperatures to mimic diurnal patterns; 0800 - 2000 h: 33.6°C, 40.0% RH, THI: 83.5; 2000 - 0800 h: 23.2°C, 70.0% RH; THI: 70.3; n = 1714 h). Data (n = 719 h) from 6 Holstein x Angus steers (487.9 ± 9.1 kg) were obtained from a subsequent experiment (Experiment 2) to confirm consistency of ear-tag accelerometer performance under elevated THI (HS conditions as described above). In Experiment 1, CM was capable of quantifying rumination time with high accuracy under TN conditions (concordance correlation coefficient [CCC]: 0.75 - 0.81). Overall, agreement between CM and the automated gold standard declined 6 - 7% during HS, which was most apparent later in the day when cattle had been subjected to HS for multiple hours (moderate agreement; CCC: 0.68). Accuracy for rumination time was also only moderate for data collected during Experiment 2 (CCC: 0.55 - 0.61). In contrast, CM reported total eating (eating with the head down + head up while masticating) time with moderate accuracy for TN (CCC: 0.53 - 0.54), only achieved negligible to low accuracy during HS (CCC: 0.39 - 0.44 [Experiment 1] and 0.17 - 0.34 [Experiment 2]). Sensor performance did improve when CM eating time was compared specifically to the time spent with the head down reported by RW; HS still negatively influenced sensor performance, however, with high agreement during TN (CCC: 0.72 - 0.73) but low to moderate agreement during HS (CCC: 0.65 - 0.69 [Experiment 1] and 0.40 - 0.58 [Experiment 2]). Results of this study suggest accuracy of ear-tag accelerometers may be impaired when cattle are subjected to heat stress.

Fecal Microbiota, Forage Nutrients, and Metabolic Responses of Horses Grazing Warm- and Cool-Season Grass Pastures.

Integrating warm-season grasses into cool-season equine grazing systems can increase pasture availability during summer months. The objective of this study was to evaluate effects of this management strategy on the fecal microbiome and relationships between fecal microbiota, forage nutrients, and metabolic responses of grazing horses. Fecal samples were collected from 8 mares after grazing cool-season pasture in spring, warm-season pasture in summer, and cool-season pasture in fall as well as after adaptation to standardized hay diets prior to spring grazing and at the end of the grazing season. Random forest classification was able to predict forage type based on microbial composition (accuracy: 0.90 ± 0.09); regression predicted forage crude protein (CP) and non-structural carbohydrate (NSC) concentrations (p < 0.0001). Akkermansia and Clostridium butyricum were enriched in horses grazing warm-season pasture and were positively correlated with CP and negatively with NSC; Clostridum butyricum was negatively correlated with peak plasma glucose concentrations following oral sugar tests (p ≤ 0.05). These results indicate that distinct shifts in the equine fecal microbiota occur in response different forages. Based on relationships identified between the microbiota, forage nutrients, and metabolic responses, further research should focus on the roles of Akkermansia spp. and Clostridium butyricum within the equine hindgut.

Crabgrass as an equine pasture forage: impact of establishment method on yield, nutrient composition, and horse preference.

Warm-season grasses (WSG) incorporated into traditional cool-season rotational grazing systems to increase summer yields are typically established in monoculture in separate pasture areas. Few studies have evaluated alternative interseeded establishment of WSG, despite potential benefits for improving biodiversity and land-use efficiency. The objective of this study was to determine the impact of establishment method (monoculture vs. interseeded) on crabgrass pasture forage yield, nutritive value, and preference under equine grazing. Three adult standardbred mares grazed two main plots on two consecutive days (8 hr/d) for three grazing events in 2019: Jul 28/29 (GRAZE 1), Aug 20/30 (GRAZE 2), Oct 1/2 (GRAZE 3). Each main plot contained four replicates of three treatments: mixed cool-season grass (CSG); Quick-N-Big crabgrass (CRB) [Digitaria sanguinalis (L.) Scop.] interseeded into existing cool-season grass (INT), and CRB established as a monoculture (MON). The cool-season grass mix included Inavale orchardgrass [Dactylis glomerata (L.)], Tower tall fescue [Lolium arundinaceum (Schreb.) Darbysh.], and Argyle Kentucky bluegrass [Poa pratensis (L.)]. Herbage mass (HM) and sward height (SH) were measured prior to each grazing event and samples were collected (0800-1000 h) for chemical composition analysis. Observed grazing time (GT) in each sub-plot as determined by 5-min scan sampling was utilized as marker of horse preference. Forage HM was greater in MON (8043 ± 1220 kg/ha) than CSG (5001 ± 1308 kg/ha; P = 0.003), with a trend for greater total HM in MON vs. INT (6582 ± 1220 kg/ha: P = 0.06), but HM did not differ between INT and CSG. The SH was also greatest for MON (28 ± 1.11; INT: 23.6 ± 1.11; CSG: 19.7 ± 1.37 cm; P < 0.003). Forage nutrients (digestible energy and crude protein) were largely similar across treatments and met requirements of horses at maintenance. Horse GT was lower in MON (22.6 ± 3.77 min/sub-plot) than in INT (31.9 ± 3.79 min/sub-plot; P = 0.003) and there was a trend for lower GT in MON vs. CSG (29.9 ± 4.17 min/sub-plot: P = 0.07). These results indicate interseeding CRB would not effectively increase yields of traditional cool-season grass equine rotational grazing systems and would not supply similar levels of summer forage provided by monoculture establishment. Results of this study also suggest horses may prefer cool-season grass pasture forage over warm-season crabgrass.

Fecal microbiome of horses transitioning between warm-season and cool-season grass pasture within integrated rotational grazing systems.

BACKGROUND: Diet is a key driver of equine hindgut microbial community structure and composition. The aim of this study was to characterize shifts in the fecal microbiota of grazing horses during transitions between forage types within integrated warm- (WSG) and cool-season grass (CSG) rotational grazing systems (IRS). Eight mares were randomly assigned to two IRS containing mixed cool-season grass and one of two warm-season grasses: bermudagrass [Cynodon dactylon (L.) Pers.] or crabgrass [Digitaria sanguinalis (L.) Scop.]. Fecal samples were collected during transitions from CSG to WSG pasture sections (C-W) and WSG to CSG (W-C) on days 0, 2, 4, and 6 following pasture rotation and compared using 16S rRNA gene sequencing. RESULTS: Regardless of IRS or transition (C-W vs. W-C), species richness was greater on day 4 and 6 in comparison to day 0 (P < 0.05). Evenness, however, did not differ by day. Weighted UniFrac also did not differ by day, and the most influential factor impacting ß-diversity was the individual horse (R2 ≥ 0.24; P = 0.0001). Random forest modeling was unable to accurately predict days within C-W and W-C, but could predict the individual horse based on microbial composition (accuracy: 0.92 ± 0.05). Only three differentially abundant bacterial co-abundance groups (BCG) were identified across days within all C-W and W-C for both IRS (W ≥ 126). The BCG differing by day for all transitions included amplicon sequence variants (ASV) assigned to bacterial groups with known fibrolytic and butyrate-producing functions including members of Lachnospiraceae, Clostridium sensu stricto 1, Anaerovorax the NK4A214 group of Oscillospiraceae, and Sarcina maxima. In comparison, 38 BCG were identified as differentially abundant by horse (W ≥ 704). The ASV in these groups were most commonly assigned to genera associated with degradation of structural carbohydrates included Rikenellaceae RC9 gut group, Treponema, Christensenellaceae R-7 group, and the NK4A214 group of Oscillospiraceae. Fecal pH also did not differ by day. CONCLUSIONS: Overall, these results demonstrated a strong influence of individual horse on the fecal microbial community, particularly on the specific composition of fiber-degraders. The equine fecal microbiota were largely stable across transitions between forages within IRS suggesting that the equine gut microbiota adjusted at the individual level to the subtle dietary changes imposed by these transitions. This adaptive capacity indicates that horses can be managed in IRS without inducing gastrointestinal dysfunction.

Diurnal Variation in Forage Nutrient Composition of Mixed Cool-Season Grass, Crabgrass, and Bermudagrass Pastures.

Warm-season grasses have been suggested as alternative low non-structural carbohydrate (NSC) pasture forages. The purpose of this study was to evaluate nutrient composition and diurnal changes in soluble carbohydrates for the warm-season annual 'Quick-N-Big' crabgrass [CRB; Digitaria sanguinalis (L.) Scop.] and the warm-season perennial 'Wrangler' bermudagrass [BER; Cynodon dactylon (L.) Pers] in comparison to mixed cool-season grass [CSG; 'Inavale' orchardgrass (Dactylis glomerata [L.]), 'Tower' tall fescue (Lolium arundinaceum [Schreb.] Darbysh.), and 'Argyle' Kentucky bluegrass (Poa pratensis [L.])]. Samples were collected at 4-hour intervals over 3 d when each forage reached the boot stage of maturity. Digestible energy was greatest for CSG (2.29 ± 0.34 Mcal/kg) and lowest for BER (2.13 ± 0.34 Mcal/kg), while crude protein was lowest for CSG (16.1 ± 0.29%) and neutral detergent fiber was greatest for BER (60.0 ± 0.41; P ≤ .0008). Non-structural carbohydrates were greater for CSG (17.6% ± 0.26%) compared to BER (10.6% ± 0.26%) or CRB (10.9% ± 0.26%; P < .0001). Overall, NSC was greatest in the afternoon and evening (14.5-14.9 ± 0.60%) and lowest in the early morning (11.2-11.4 ± 0.60%; P ≤ .04), but diurnal variation was most pronounced in CSG versus either Warm-season grasses. Results of this study provide needed data on nutritional composition of CRB and BER and demonstrate that these grasses may serve as pasture forages for horses where NSC intake is of concern. Results also support recommendations for restricting grazing to early morning to limit NSC consumption, particularly in CSG pastures.

Yield, nutrient composition, and horse condition in integrated crabgrass and cool-season grass rotational grazing pasture systems.

Integration of warm-season grasses into traditional cool-season pastures can increase summer forage for grazing cattle. The aim of this study was to determine impacts of this practice on yield and nutrient composition of equine rotational pasture systems as well as horse body condition. Two 1.5 ha rotational systems (6 to 0.25 ha sections/system) were evaluated: a control system (CON) (all sections mixed cool-season grass [CSG-CON]) and an integrated rotational grazing system (IRS) (three CSG sections [CSG-IRS] and three Quick-N-Big crabgrass [Digitaria sanguinalis (L.) Scop.; CRB-IRS]). Three horses per system grazed in three periods: EARLY (mid-May to mid-July), SLUMP (mid-July to mid-September), and LATE (mid-September to mid-November). Herbage mass (HM) was measured prior to each rotation and samples were collected (0800 to 1000 h) for nutrient analysis. Grazing days were tracked to calculate carrying capacity (CC). Horse condition measures were assessed monthly. Over the full grazing season, 9,125 kg of forage was available for grazing in IRS versus 6,335 kg in CON. The CC was 390 horse d for IRS, while only 276 horse d for CON. Total HM/section did not differ during EARLY when CRB was not available (CSG-IRS: 2,537 ± 605; CSG-CON: 3,783 ± 856 kg/ha), but CC was greater in CSG-IRS (220 ± 37 horse d/ha) than CSG-CON (92 ± 26 horse d/ha; P = 0.03). In SLUMP, both HM and CC were greater in CRB-IRS (HM: 4,758 ± 698 kg/ha; CC: 196 ± 31 horse d/ha) than CSG-IRS (HM: 1,086 ± 698 kg/ha; CC: 32 ± 31 horse d/ha) or CON (HM: 970 ± 493 kg/ha; CC: 46 ± 22 horse d/ha; P < 0.02). While HM did not differ by section type in LATE (1,284 ± 158 kg/ha), CC was greater in CSG-CON (84 ± 9 horse d/ha) versus CRB-IRS (32 ± 13 horse d/ha; P = 0.03) and CSG-IRS (40 ± 13 horse d/ha; P = 0.06). During SLUMP, water-soluble carbohydrates (WSC) were lower in CRB-IRS (4.46% ± 0.80%) than CSG-CON (7.92% ± 0.90%; P < 0.04), but not CSG-IRS (5.93% ± 1.04%); however, non-structural carbohydrates (NSC) did not differ (7.05% ± 0.62%). There were no differences in WSC (6.46% ± 0.54%) or NSC (7.65% ± 0.54%) by section type in LATE. Horses in IRS maintained a body condition score (BCS) of 5.78 ± 0.48, but BCS did not differ by system (CON: 6.11 ± 0.48). Thus, integrated grazing increased summer pasture yield and provided adequate nutrition to maintain horse condition, but further research is needed to improve late-season production. Integrated grazing may not, however, provide an advantage in limiting dietary NSC, as NSC remained low for all pasture sections.

Effects of 27 mo of rotational vs. continuous grazing on horse and pasture condition.

The objective of this study was to determine whether rotational grazing generates horse, pasture, or cost benefits over continuous grazing. The study established two replicates (1.57 ha each) of rotational (R; four grazing sections and a stress lot per replicate, where horses were fed a moderate quality grass hay at 2% of body weight when not grazing) and continuous (C) grazing systems (treatments). Twelve Standardbred mares were grazed for an overall stocking rate of 0.52 ha/horse (n = 3 in each pasture). Recommended management practices for each grazing system were followed for 27 mo including three grazing seasons. Samples were collected monthly between 0800 and 1000. Results were analyzed in SAS (V9.4) using mixed model repeated-measures analysis of covariance, chi-square tests of association, and two-sample t-tests. Alpha level was set at P < 0.05. The C horses were maintained on pasture for 100% of the study duration (844 d; August 1, 2014 to November 22, 2016), while R horses had access to pasture for approximately half of this time (408 ± 33 d). The average length of grazing bout per rotational grazing section during the grazing season increased numerically each year from 7.88 ± 0.76 d in 2014, 10.0 ± 0.61 d in 2015, and 10.9 ± 0.80 d in 2016. Average horse body condition score (BCS) and body fat differed by treatment, with C horses (BCS 6.3 ± 0.05, 17.9 ± 0.15% body fat) greater than R horses (BCS 5.9 ± 0.05, 16.8 ± 0.15% body fat). Both sward height and herbage mass were greater in R (11.8 ± 0.1 cm tall; 1,513 ± 41 kg/ha) than C pastures (6.9 ± 0.1 cm tall; 781 ± 35 kg/ha). The R pastures had higher proportions of vegetative and total cover, planted grasses (tall fescue and orchardgrass), and weeds but lower proportions of grass weeds (nonplanted grasses) and other (rocks, litter, bare ground, etc.) as compared with C pastures. Digestible energy, acid detergent fiber, and calcium were higher in R vs. C pastures; however, crude protein was lower in R vs. C pastures. There were no significant differences between treatments for average monthly amount of hay fed (C, 597 ± 34.1 vs. R, 659 ± 34.1 kg) or average monthly pasture maintenance cost (C, $17.55 ± 3.14 vs. R, $20.50 ± 3.14). This study is one of few replicated experiments comparing the effects of rotational and continuous grazing for horses on pasture quality, horse condition, and production costs. The results here support the recommendation of rotational grazing for production, environmental, and ecological purposes.

Validation and Implementation of an Automated Chew Sensor-Based Remote Monitoring Device as Tool for Equine Grazing Research.

Field studies characterizing equine grazing activity primarily rely on observational protocols, limiting the quantity and accuracy of collected data. The objectives of this study were to validate an automated chew sensor technology, the EquiWatch System (EWS), for detecting grazing behaviors and to demonstrate potential applications of the EWS in equine grazing research. Eight mature standardbred mares were used in this study. EquiWatch System validation was completed in two phases: grazing time was evaluated in experiment 1 and chew counts in experiment 2. The correlation between visual observations and system-recorded grazing time was high (concordance correlation coefficient [CCC] = 0.997). There was also a high agreement between the sum of manually counted bites and chews and total chew counts reported by the EWS (CCC = 0.979). Following validation, a pilot study was conducted using the EWS to assess feeding behaviors of horses with unrestricted pasture access (PAS) versus horses offered ad libitum hay (HAY). Horses spent more time engaged in feeding behavior on PAS (14.79 ± 0.48 hr/d) than HAY (11.98 ± 0.48 hr/d; P < .0001). Chewing rate also differed by forage (PAS 83.92 ± 1.61; HAY 68.50 ± 1.61 chews/min; P < .0001). However, although the magnitude of these behavioral parameters was influenced by treatment, the underlying 24-hour patterns were largely preserved regardless of forage type. These results demonstrate that the EWS can generate data necessary for characterizing feeding behavior in horses. Future studies implementing this tool could provide a greater understanding of biological, environmental, and nutritive factors driving grazing behavior in horses.

Effects of grazing system, season, and forage carbohydrates on glucose and insulin dynamics of the grazing horse.

Rotational grazing is a recommended practice for grazing livestock, but little is known about its benefits with respect to grazing horses. The objective of this study was to investigate the effects of continuous (CON) and rotational (ROT) grazing on forage nutrient composition and whether those concentrations influenced circulating glucose and insulin concentrations in the grazing horse. Twelve mature Standardbred mares were paired by age and weight and randomly assigned to 1 replicate of either a 1.5 ha cool-season grass CON or ROT system for a total of 3 mares in each system. Mares on CON were allowed to graze the entire system at all times, whereas mares on ROT were given access to a 0.4 ha pasture section and stress lot where they were confined during inclement weather and slow forage growth. Blood and feces from horses and forage from each system were sampled over one 24-h period in June, August, and October. Blood was assessed for plasma glucose (GLU) and serum insulin (INS), feces for pH, and forage for nutritional composition. Data were analyzed by ANOVA with repeated measures with significance set at P < 0.05. There were no treatment differences for water and ethanol soluble carbohydrates (WSC and ESC, respectively), starch, ADF, and NDF, but CP was lower in ROT compared to CON (P = 0.04). With respect to month, WSC were highest in June compared to August and October, whereas ESC were highest in June compared to only August. Starch was lower in October than in June and August. Concentrations of ADF and NDF were lowest in October compared to June and August. Crude protein was higher in October than June and August. Plasma GLU and serum INS were affected by season and time of day but not grazing system. For all horses, GLU was highest in August (105.6 ± 1.3 mg/dL), whereas INS was highest in October (0.21 ± 0.02 µg/L; P < 0.0001). Fecal pH only varied by season and was highest in August (7.06; P < 0.0001). Few consistent correlations between grazing systems were found with the exception of INS with ESC (R = 0.32 to 0.39; P < 0.04) and INS and GLU with ADF and NDF in August and October (R = -0.31 to -0.48; P < 0.04). In conclusion, grazing system did not affect the forage carbohydrate concentrations or GLU or INS in horses; however, season did have an effect on both forage nutrient content and glucose metabolism in horses.

Technical note: Comparing 4 techniques for estimating desired grass species composition in horse pastures.

Many methods exist for estimating species composition, but few studies compare those useful in improved horse pastures. The objective of this study was to examine 4 techniques for estimating desirable forage species composition in 2 cool-season horse pastures based on prevalence estimates, repeatability, bias, and practicality, and to select a method for use in a subsequent grazing study. The techniques included Equine Pasture Evaluation Disc (EPED), Line-Point Intercept with 3 transects of 50 observations each (LPI 3-50), LPI with 5 transects of 30 observations each (LPI 5-30), and Step Point (StPt). A generalized linear-mixed effects model procedure of SAS (GLIMMIX) with a logit link was used to test for differences among each species separately. When methods were significantly different (α = 0.05), pairwise comparisons were performed using a paired t-test. The methods did not differ in detecting creeping bentgrass (P = 0.3334) or orchardgrass (P = 0.4207), but there were differences for Kentucky bluegrass (P = 0.0082), tall fescue (P = 0.0314), and other (P = 0.0448). Repeatability plots displayed lower method repeatability as species prevalence increased. Agreement was analyzed between pairs of methods by grass species. Five out of 30 pairs showed significant overall bias (P = 0.0114, 0.0045, 0.0170, 0.0328, and 0.0404), and 3 of them were between LPI 3-50 and EPED. The LPI 3-50 and LPI 5-30 techniques agreed perfectly in prevalence and bias, as did StPt and EPED, meaning they can be used interchangeably. The techniques LPI 3-50 and EPED were the most dissimilar methods. In conclusion, StPt can be used interchangeably with LPI, but StPt was selected due to its thorough representation of the pastures and ease of use.

Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses.

Objectives were to evaluate effects of (1) repetitive arthrocentesis on biomarkers of inflammation (prostaglandin E(2), PGE(2)) and aggrecan synthesis (chondroitin sulfate-846; CS) in synovial fluid (SF); (2) exercise and superoxide dismutase (SOD) supplementation on biomarkers of inflammation, antioxidant status, and aggrecan synthesis, in horses. Preliminary trial. Standardbreds underwent four arthrocentesis procedures within 48 h and exhibited elevated CS and no changes in PGE(2). Exercise trial. this randomized crossover design used twelve Standardbred mares which received either treatment (3000 IU d(-1) oral SOD powder) or placebo (cellulose powder) for 6 wks which culminated with them running a repeated sprint exercise test (RSET). Samples were collected before (PRE), during (PEAK), and following exercise (POST). Exercise resulted in increased (P < 0.05) antioxidant defenses including erythrocyte SOD, total glutathione, glutathione peroxidase, gene transcripts for interferon-gamma, interleukin-10, and interleukin-1ß in blood, and decreased plasma nitric oxide. Exercise increased (P < 0.05) SF CS and adjusted-PGE(2), and higher (P < 0.05) CS and PGE(2) were found in hock versus carpus joints. No treatment effects were detected. Results suggest normal adaptive responses likely due to exercise-induced tissue microdamage and oxidative stress. Additional research is needed to identify benefit(s) of SOD supplementation in horses.

Antioxidant status in elite three-day event horses during competition.

The objective of this study was to determine if competition intensity would have an effect on antioxidant status in horses before and during a three-day event. Body weight, body condition score, and blood was sampled from CCI2* (n = 19) and CCI3* (n = 23) horses before the start of dressage, 20 to 30 min following cross-country, and 18-24 h after cross-county. Data were analyzed using a PROC MIXED in SAS. There were no differences between CCI2* and CCI3* horses during competition for plasma cortisol, lactate, α-tocopherol, retinol, or erythrocyte glutathione peroxidase. After cross-country, CCI3* horses had higher serum creatine kinase (P = 0.003) and aspartate aminotransferase (P < 0.0001) than the CCI2* horses. Plasma ß-carotene was higher in the CCI2* horses compared to the CCI3* horses (P = 0.0001). Total erythrocyte glutathione was also higher in the CCI2* horses versus CCI3* horses (P < 0.0001). These results are the first report of antioxidant status of horses competing in this level of a three-day event. The changes in antioxidant and muscle enzymes observed between divisions are likely due to the increased anaerobic and musculoskeletal demand on the upper level horses and the fitness required to compete at that level.

Nutritional supplementation, performance, and oxidative stress in college soccer players.

The purpose of this study was to examine changes in performance and metabolic parameters in collegiate soccer players during preseason preparation and to determine the impact of a nutraceutical blend proposed to reduce oxidative stress. Male Division I college soccer players (n = 22) performed a progressive maximal treadmill test at the beginning and end of preseason to assess changes in Vo2max, velocity at lactate threshold (VLT), time-to-exhaustion, lipid hydroperoxide (LPO), 8-isoprostane, and creatine kinase (CK) response. After baseline testing, athletes were randomly assigned to receive the nutraceutical blend (EXP; n = 12) or an isocaloric equivalent (CON; n = 10) for 20 days of preseason training. DeltaVo2max (2.1 +/- 3.3 ml.kg.min, p = 0.007), DeltaVLT (0.8 +/- 1.4 km.h, p = 0.045), and Deltatime-to-exhaustion (39.4 +/- 77.4 seconds, p = 0.033) were improved across groups, but a significant effect of supplementation on performance was not seen. Changes in resting levels of CK from the beginning to end of preseason were significantly lower (p = 0.044) in EXP (64.8 +/- 188.4 U.L) than in CON (292.8 +/- 304.8 U.L). Additionally, EXP demonstrated a significant decrease in the magnitude of the 8-isoprostane response at Trial 2 compared with Trial 1 (effect size [ES] = -0.74), whereas CON had an increased response (ES = 0.20). A similar pattern was seen for LPO (p = 0.067). Preseason training in male college soccer players resulted in significant improvements in Vo2max, VLT, and time-to-exhaustion. Supplementing with a proprietary antioxidant and nutraceutical blend may enhance some of these effects as indicated by magnitude of the responses. However, it appears that the most notable effects of supplementation were seen for reduced CK and oxidative stress, at least with short-term supplementation.

Some commonly fed herbs and other functional foods in equine nutrition: a review.

Most herbs and functional foods have not been scientifically tested; this is especially true for the horse. This paper reviews some of the literature pertinent to herbal supplementation in horses and other species. Common supplements like Echinacea, garlic, ginger, ginseng, and yucca are not regulated, and few studies have investigated safe, efficacious doses. Ginseng has been found to exert an inhibitory effect on pro-inflammatory cytokines and cyclooxygenase-2 expression. Equine studies have tested the anti-inflammatory effects of a single dose of ginger, post-exercise. Echinacea has been reported to have anti-inflammatory and antioxidant properties. Yucca contains steroid-like saponins, which produce anti-inflammatory, antioxidant, and anti-spasmodic effects. However, some herbs have drug-like actions that interact with dietary components and may contain prohibited substances like salicylates, digitalis, heroin, cocaine and marijuana. Horses fed garlic at >0.2g/kg per day developed Heinz body anaemia. Drug-herb interactions are common and caution needs to be taken when implementing 'natural product' usage.

Temporal folate status during lactation in mares and growth in foals.

OBJECTIVE: To identify changes in folate status of mares and foals during lactation and growth, respectively. ANIMALS: 20 Thoroughbred mares and foals. PROCEDURES: Pregnant mares, and following foaling the same mares with their foals, were maintained on mixed grass-legume pasture and fed either a traditional dietary supplement rich in sugar and starch (SS) or a dietary supplement high in fat and fiber (FF). Blood samples were collected monthly from mares and foals up to 6 months after foaling. Total folate concentration in feed and forage was determined. Analyses of plasma folate, RBC folate, plasma homocysteine (HCY), and milk folate concentrations were performed. RESULTS: Mare plasma folate concentrations declined moderately during 6 months of lactation. Mare RBC folate concentrations initially increased after foaling up to 3 months but declined toward the end of the study. Plasma HCY concentration was higher for mares fed the SS supplement, compared with mares fed the FF supplement from foaling to 6 months of lactation. Milk folate concentrations decreased during the first 3 months and then increased. Foal plasma folate initially declined but then increased. Stable concentrations of RBC folate were observed in foals. Plasma HCY concentrations in foals were unaffected by growth during the last 5 months. References range values for plasma folate, RBC folate, milk folate, and plasma HCY concentrations in healthy lactational mares and young growing foals were determined. CONCLUSIONS AND CLINICAL RELEVANCE: Folate status was not impaired in lactating mares and growing foals under the conditions in our study. It appears that folate supplementation is not necessary.

Effects of oral potassium supplementation on acid-base status and plasma ion concentrations of horses during endurance exercise.

OBJECTIVE: To compare effects of oral supplementation with an experimental potassium-free sodium-abundant electrolyte mixture (EM-K) with that of oral supplementation with commercial potassium-rich mixtures (EM+K) on acid-base status and plasma ion concentrations in horses during an 80-km endurance ride. ANIMALS: 46 healthy horses. PROCEDURE: Blood samples were collected before the ride; at 21-, 37-, 56-, and 80-km inspection points; and during recovery (ie, 30-minute period after the ride). Consumed electrolytes were recorded. Blood was analyzed for pH, PvCO2, and Hct, and plasma was analyzed for Na+, K+, Cl-, Ca2+, Mg2+, lactate, albumin, phosphate, and total protein concentrations. Plasma concentrations of H+ and HCO3-, the strong ion difference (SID), and osmolarity were calculated. RESULTS: 34 (17 EM-K and 17 EM+K treated) horses finished the ride. Potassium intake was 33 g less and Na+ intake was 36 g greater for EM-K-treated horses, compared with EM+K-treated horses. With increasing distance, plasma osmolarity; H+, Na+, K+, Mg2+, phosphate, lactate, total protein, and albumin concentrations; and PvCO2 and Hct were increased in all horses. Plasma HCO3-, Ca2+, and Cl- concentrations were decreased. Plasma H+ concentration was significantly lower in EM-K-treated horses, compared with EM+K-treated horses. Plasma K+ concentrations at the 80-km inspection point and during recovery were significantly less in EM-K-treated horses, compared with EM+K-treated horses. CONCLUSIONS AND CLINICAL RELEVANCE: Increases in plasma H+ and K+ concentrations in this endurance ride were moderate and unlikely to contribute to signs of muscle fatigue and hyperexcitability in horses.

Lipoic acid as an antioxidant in mature thoroughbred geldings: a preliminary study.

alpha-Lipoic acid (LA) has demonstrated antioxidant effects in humans and laboratory animals. The objective of this study was to determine whether the effects of LA are similar in horses. Five Thoroughbred geldings were supplemented with 10 mg/kg/d DL-alpha-lipoic acid in a molasses and sweet feed carrier and five received only the carrier as a placebo (CON). Blood samples were obtained at baseline (0 d), after 7 and 14 d of supplementation, and 48 h postsupplementation (16 d). Blood fractions of red and white blood cells (RBC and WBC, respectively) and plasma were analyzed for glutathione (GSH), glutathione peroxidase (GPx) and total plasma lipid hydroperoxides (LPO). An experienced veterinarian observed no adverse clinical effects. Plasma LPO baselines differed between groups (P = 0.002). When covariates were used, there was a decrease over time in the LA group (P = 0.015) and concentrations were lower in the LA group than in the CON group at 7 and 14 d (P = 0.022 and P = 0.0002, respectively). At baseline, GSH concentration was 69 +/- 7 in WBC and 115 +/- 13 mmol/mg protein in the RBC, with no differences resulting from either time or treatment. The GPx activity was 47 +/- 4 and 26 +/- 5 U/g protein at baseline WBC and RBC, respectively, with a lower concentration in the LA group's WBC at 7 (P = 0.019) and 14 d (P = 0.013). The results show that 10 mg/kg LA had no evident adverse effects, and moderately reduced the oxidative stress of horses allowed light activity. These findings encourage studying of LA in horses subjected to strenuous exercise.