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BACKGROUND: Efficacy testing of immunotherapy in field studies is often hampered by variation of airborne allergens. Standardized allergen exposure in challenge chamber settings might be an alternative. Therefore, we developed a universal technique to create an atmosphere loaded with allergen particles of adjustable size from aqueous solutions of licensed allergen extracts. OBJECTIVE: The aim of this study was to apply this technique and test the safety and efficacy of challenges with house dust mite (HDM) allergen in the Fraunhofer allergen challenge chamber. METHODS: Aerosol particles carrying HDM allergen were produced by spray-drying of an aqueous solution containing HDM allergen and lactose. In a monocenter, placebo-controlled, single-blind, dose-escalation pilot study, 18 subjects with perennial allergic rhinitis and sensitization to HDM were exposed to HDM allergen for 4 h at either 250, 500, 1000 SQE/m3 or lactose alone (0 SQE/m3 ) 7 days apart. The dose of 500 SQE/m³ was repeated to investigate reproducibility. Total nasal symptom score (TNSS) was the primary endpoint. RESULTS: Exposure to HDM increased TNSS (mean ± SD) to 3.4 ± 1.8, 3.3 ± 2.1, and 3.6 ± 2.0 at 250, 500 and 1000 SQE/m3 , respectively, while lactose alone did not change TNSS (0.7 ± 0.6). The results were reproducible at 500 SQE/m3 . Pulmonary function and adverse event frequency did not change with escalation of allergen dose. CONCLUSION: This HDM allergen particle generation is safe, specific and reproducible and can therefore be used for efficacy testing of immunotherapy and for basic clinical research.
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Antígenos Dermatofagoides/inmunología , Inmunización , Pyroglyphidae/inmunología , Adulto , Animales , Antígenos Dermatofagoides/administración & dosificación , Antígenos Dermatofagoides/efectos adversos , Desensibilización Inmunológica , Espiración , Femenino , Humanos , Inmunización/métodos , Inmunoglobulina E/inmunología , Masculino , Persona de Mediana Edad , Mucosa Nasal/inmunología , Mucosa Nasal/metabolismo , Óxido Nítrico/metabolismo , Pruebas de Función Respiratoria , Rinitis Alérgica/diagnóstico , Rinitis Alérgica/inmunología , Rinitis Alérgica/terapia , Factores de Riesgo , Adulto JovenRESUMEN
BACKGROUND: In pulmonary medicine, aerosolization of substances for continuous inhalation is confined to different classes of nebulizers with their inherent limitations. Among the unmet medical needs is the lack of an aerosolized surfactant preparation for inhalation by preterm neonates, to avoid the risks associated with endotracheal intubation and surfactant bolus instillation. In the present report, we describe a high-concentration continuous powder aerosolization system developed for delivery of inhalable surfactant to preterm neonates. METHODS: The developed device uses a technique that allows efficient aerosolization of dry surfactant powder, generating a surfactant aerosol of high concentration. In a subsequent humidification step, the heated aerosol particles are covered with a surface layer of water. The wet surfactant aerosol is then delivered to the patient interface (e.g., nasal prongs) through a tube. RESULTS: The performance characteristics of the system are given as mass concentration, dose rate, and size distribution of the generated aerosol. Continuous aerosol flows of about 0.84 L/min can be generated from dry recombinant surfactant protein-C surfactant, with concentrations of up to 12 g/m(3) and median particle sizes of the humidified particles in the range of 3 to 3.5 µm at the patient interface. The system has been successfully used in preclinical studies. CONCLUSION: The device with its continuous high-concentration delivery is promising for noninvasive delivery of surfactant aerosol to neonates and has the potential for becoming a versatile disperser platform closing the gap between continuously operating nebulizers and discontinuously operating dry powder inhaler devices.
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Recien Nacido Prematuro , Nebulizadores y Vaporizadores , Proteína C Asociada a Surfactante Pulmonar/administración & dosificación , Surfactantes Pulmonares/administración & dosificación , Síndrome de Dificultad Respiratoria del Recién Nacido/tratamiento farmacológico , Administración por Inhalación , Aerosoles , Química Farmacéutica , Diseño de Equipo , Humanos , Humedad , Recién Nacido , Ensayo de Materiales , Tamaño de la Partícula , Polvos , Proteína C Asociada a Surfactante Pulmonar/química , Surfactantes Pulmonares/química , Proteínas Recombinantes/administración & dosificaciónRESUMEN
To contribute to the social debate about the role of functional foods in the prevention of overweight and obesity using an ecological model to study the positioning of functional foods and their social implications. Positioning was conceptualized as the relative attention given to functional foods within the range of preventive strategies, and the way in which they address specific causes of overweight. A systematic review was conducted to identify (A) preventive strategies aiming at the individual; (B) technological approaches; and (C) environmental strategies. All strategies were further classified according to the nature of causes they refer to - either individual or environmental. In the prevention of overweight/obesity, an emphasis on strategies designed to change the quality of food products and supplies has developed. Technological strategies particularly relate to functional foods; however, while providing a new dimension to food products, they do not challenge the underlying lifestyles causing overweight. Furthermore, they also stress individual responsibility for overweight/obesity and technological solutions to it. From a societal perspective, the characteristics of functional foods indicate that they can only be expected to play a limited role in overweight/obesity prevention. The ecological approach suggests that other strategies targeting individual and social causes need to be developed and marketed equally well.
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Tecnología de Alimentos , Alimentos Orgánicos , Obesidad/prevención & control , Sobrepeso/prevención & control , Humanos , Estilo de Vida , Valor NutritivoRESUMEN
BACKGROUND: There is an increasing need for allergen inhalation systems to perform basic clinical research and test anti-allergic drugs under well-controlled conditions. This requires stability of environmental conditions like temperature and humidity, as well as allergen concentration and reproducible induction of allergic symptoms. OBJECTIVE: The aim of this study was to validate an environmental exposure unit for controlled human pollen inhalation studies in participants with seasonal allergic rhinitis. METHODS: Temperature, relative humidity, and air flow rate were kept constant with an air conditioning system. Pollen atmosphere was generated using a specially designed feeding system and monitored online by laser counter and offline using rotating rod samplers. Efficacy (total nasal symptom score, nasal air flow rate, nasal secretion) and safety (lung function) parameters were evaluated at different pollen concentrations and repeated allergen challenges. RESULTS: Temperature, humidity, and air flow rate in the environmental exposure unit remained constant within a range of <2%. The spatial distribution and the temporal stability of the pollen concentration varied only slightly over 4 h (+/-10% and <4%, respectively). Dose-dependent induction of allergic rhinitis symptoms, reduction in nasal air flow rate, and increase in nasal secretion were observed over time. These effects were reproducible from day to day. Lung function remained clinically normal at all concentrations and from day to day. CONCLUSIONS: Thus, pollen exposure in the environmental exposure unit is an effective, reproducible, safe, and suitable method for single-centre clinical studies on the efficacy of anti-allergic treatment or basic clinical research.
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Alérgenos/análisis , Exposición a Riesgos Ambientales , Pruebas de Provocación Nasal/instrumentación , Poaceae , Polen , Rinitis Alérgica Estacional/inmunología , Adulto , Hiperreactividad Bronquial , Femenino , Humanos , Masculino , Pruebas de Provocación Nasal/métodos , Factores de TiempoRESUMEN
A method for inhalative exposure of minipigs to aerosols and gases has been developed. Minipigs are exposed via mask inhalation to the test substance using a computer-controlled exposure system that permits simultaneous exposure of groups of four animals in parallel to different controlled dose levels. We studied inhalation treatment of verapamil, a cardiovascular drug, and show good absorption and favorable pharmacokinetics when compared with iv drug application. The results shown in this study encourage inhalation studies with the Göttingen minipig.
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Bloqueadores de los Canales de Calcio/farmacocinética , Exposición por Inhalación , Porcinos Enanos/metabolismo , Verapamilo/farmacocinética , Aerosoles , Animales , Bloqueadores de los Canales de Calcio/administración & dosificación , Bloqueadores de los Canales de Calcio/sangre , Estudios Cruzados , Femenino , Inyecciones Intravenosas , Pulmón/fisiología , Masculino , Porcinos , Verapamilo/administración & dosificación , Verapamilo/sangreRESUMEN
Within the frame of inprocess analytics of the synthesis of pharmaceutical drugs a lot of HPLC methods are required for checking the quality of intermediates and drug substances. The methods have to be developed in terms of optimal selectivity and low limit of detection, minimum running time and chromatographic robustness. The goal was to shorten the method development process. Therefore, the screening of stationary phases was automated by means of switching modules equipped with 12 HPLC columns. Mobile phase and temperature could be optimized by using Drylab after evaluating chromatograms of gradient elutions performed automatically. The column switching module was applied for more than three dozens of substances, e.g. steroidal intermediates. Resolution (especially of isomeres), peak shape and number of peaks turned out to be the criteria for selection of the appropriate stationary phase. On the basis of the "best" column the composition of the "best" eluent was usually defined rapidly and with less effort. This approach leads to savings in manpower by more than one third. Overnight, impurity profiles of the intermediates were obtained yielding robust HPLC methods with high selectivity and minimized elution time.
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Cromatografía Líquida de Alta Presión/métodos , Autoanálisis , Esteroides/análisisRESUMEN
Previous investigations on the effects of cigarette smoke on cultured cells have used mainly smoke condensate dissolved in culture medium. A system has been designed which allows direct exposure of cells to fresh cigarette smoke, without an intervening layer of growth medium between the cells and the smoke. Preliminary results have been obtained which demonstrate the viability of the system. V79 cells were cultured on porous membranes (Transwell; Costar). During smoke exposure only the lower surface of each Transwell is supplied with culture medium from the bottom of the culture chambers. In this way the cells had direct contact with the atmosphere at the upper surface and could be exposed directly to the test compound. The constructed exposure system consists of a smoke generator and an exposure unit containing six Transwells, the latter contained in an incubator. Cigarette smoke was generated using a standard 2 s, 35 ml puff once per min. The puff is diluted with conditioned air from the incubator and injected into the exposure unit. Following exposure of the cells to air only for 3 h there was no effect upon V79 cell viability. However, after exposure to smoke containing between 88 and 224 mg/m3 particulate matter, an inhibition of cell proliferation and induction of micronuclei was measured. When a Cambridge filter pad was placed between the cigarette and the cell exposure system to remove particulate matter cell proliferation was also reduced and an increased frequency of micronuclei above the control value was measured.
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Micronúcleos con Defecto Cromosómico/efectos de los fármacos , Contaminación por Humo de Tabaco/efectos adversos , Aerosoles , Aire , Animales , Cámaras de Exposición Atmosférica , Línea Celular , Cricetinae , Pulmón/citología , Pruebas de Micronúcleos/instrumentación , Pruebas de Micronúcleos/métodos , Tamaño de la PartículaRESUMEN
In order to outline basic concepts for the design of a bacterial aerosol infection model, the development of a pig model with Actinobacillus pleuropneumoniae is described. First, reproducibility of aerosol parameters should be maintained by optimizing generating and sampling conditions. Survival rates of the chosen strain must be predictable. Secondly, inhalation conditions for the recipients have to be standardized to enable the determination of deposition sites and the dose administered. Subsequently, dose-response relationship should be evaluated to find a suitable challenge dose. Furthermore, it seems necessary to establish methods to obtain local specimens for determination of the local immune responses. The present study demonstrates that after aerosol challenge pigs were completely protected after inhalation and partially protected after oral application of A. pleuropneumoniae vaccines and describes techniques to administer bacteria in a dose-dependent, viable way. Using the infection model several stages of the disease from acute pleuropneumonia to chronic infection can be induced for research purposes.
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Infecciones por Actinobacillus/inmunología , Infecciones por Actinobacillus/veterinaria , Actinobacillus pleuropneumoniae , Vacunas Bacterianas/administración & dosificación , Enfermedades de los Porcinos , Infecciones por Actinobacillus/prevención & control , Actinobacillus pleuropneumoniae/inmunología , Administración por Inhalación , Administración Oral , Aerosoles , Animales , Líquido del Lavado Bronquioalveolar , Relación Dosis-Respuesta a Droga , Humanos , Esquemas de Inmunización , Linfocitos/inmunología , Reproducibilidad de los Resultados , PorcinosAsunto(s)
Infecciones por Actinobacillus/veterinaria , Actinobacillus pleuropneumoniae/inmunología , Inmunización/métodos , Pleuroneumonía/veterinaria , Enfermedades de los Porcinos/prevención & control , Infecciones por Actinobacillus/inmunología , Infecciones por Actinobacillus/prevención & control , Actinobacillus pleuropneumoniae/patogenicidad , Administración Oral , Aerosoles , Animales , Anticuerpos Antibacterianos/biosíntesis , Vacunas Bacterianas/administración & dosificación , Líquido del Lavado Bronquioalveolar/inmunología , Inmunidad Mucosa , Pulmón/inmunología , Masculino , Pleuroneumonía/inmunología , Pleuroneumonía/prevención & control , Porcinos , Enfermedades de los Porcinos/inmunologíaRESUMEN
A new cluster arousal analysis system was developed to quantify microdisturbances in the sleep architecture. The system was evaluated in 10 healthy volunteers and the sleep of 23 outpatients with chronic low back pain was investigated before and after a special rehabilitative training. The comparison of sleep parameters before and after the treatment showed a significant reduction of the cluster disturbed sleep (CDS), whereas sleep efficiency and the number of stage shifts remained unchanged. The reduction of the CDS correlated with a reduction of depression scale. This cluster arousal analysis appears to be a sensitive tool for evaluating alterations in the sleep microstructure.
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A series of 113 consecutive patients underwent surgical treatment for a supratentorial meningioma between January 1955 and January 1975. There were 73 women and 40 men. There were 10 postoperative deaths (8.8%). The surgical procedure was graded according to Simpson's classification. 18 patients had a recurrence (17.5%). Women had a preponderance for recurrence. In our series the recurrence rate was 13 for women (17.8%) and 5 for men (12.5%). There was no correlation with the age of the patient or site of the tumour. The grade of initial surgery and the histology of the tumour were shown to be important.