RESUMEN
AIMS: Atopic dermatitis (AD) is marked by elevated levels of immunoglobulin E and skin lesions such as oedema and haemorrhage. Kimchi is a Korean fermented food that contains beneficial bacteria for human health. In this study, Lactobacillus plantarum CJLP55, CJLP56, CJLP133 and CJLP136 isolated from Kimchi were investigated for their capacity to inhibit AD. METHODS AND RESULTS: The three strains, CJLP55, CJLP133 and CJLP136, suppressed AD-like skin lesions, high serum IgE levels and epidermal thickening. The three strains diminished the accumulation of eosinophils and mast cells into topical inflammatory sites and the enlargement of axillary lymph nodes, which are responsible for the dorsal dermatitis. CJLP55, CJLP133 and CJLP136 decreased production of type 2 cytokines such as IL-4 and IL-5 in lymph node cell culture. CJLP133 and CJLP136 increased IFN-γ secretion, while CJLP55 enhanced IL-10 production. CONCLUSIONS: The three strains isolated from Kimchi suppress house-dust mite-induced dermatitis in NC/Nga mouse, a representative animal model of human AD. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings suggest that lactobacilli isolated from Kimchi inhibit AD, probably by altering the balance of Th1/Th2 ratio or inducing IL-10 production.
Asunto(s)
Dermatitis Atópica/terapia , Microbiología de Alimentos , Lactobacillus plantarum/aislamiento & purificación , Probióticos , Piel/patología , Administración Oral , Animales , Brassica/microbiología , Dermatitis Atópica/inmunología , Dermatitis Atópica/patología , Modelos Animales de Enfermedad , Eosinófilos/inmunología , Femenino , Fermentación , Inmunoglobulina E/sangre , Inflamación/patología , Interferón gamma/biosíntesis , Interleucina-10/biosíntesis , Interleucina-4/biosíntesis , Interleucina-5/biosíntesis , Ganglios Linfáticos/patología , Mastocitos/inmunología , Ratones , PyroglyphidaeRESUMEN
Ginsenoside Rg3, which is obtained as a by-product during the steaming of red ginseng, at 300 microg/ml enhanced the proliferation of the total spleen and bone marrow (BM) cells in both the cyclophosphamide (CYC)-treated and non-CYC-treated groups.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , División Celular/efectos de los fármacos , Ginsenósidos/farmacología , Panax , Fitoterapia , Extractos Vegetales/farmacología , Bazo/efectos de los fármacos , Animales , Antineoplásicos Fitogénicos/administración & dosificación , Antineoplásicos Fitogénicos/uso terapéutico , Células de la Médula Ósea/efectos de los fármacos , Ciclofosfamida , Cartilla de ADN , Ginsenósidos/administración & dosificación , Ginsenósidos/uso terapéutico , Masculino , Ratones , Ratones Endogámicos ICR , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Reacción en Cadena de la Polimerasa , Bazo/citologíaRESUMEN
The transfer of a hydrogen atom-a proton and an electron-is a fundamental process in chemistry and biology. A variety of hydrogen atom transfer reactions, involving iron complexes, phenols, hydroxylamines, tBuOOH, toluene, and related radicals, are shown to follow the Marcus cross relation. Thus, the Marcus theory formalism based on ground-state energetics and self-exchange rates, originally developed for electron transfer processes, is also valuable for hydrogen atom transfer. Compounds that undergo slow proton transfer (C-H bonds) or slow electron transfer (cobalt complexes) also undergo slow hydrogen atom transfer. Limitations of this approach are also discussed.