RESUMEN
To investigate the mechanism of antiprogestins in the regulation of ovarian function, a dual-chamber culture system was prepared with the amnion membrane of human placenta. Isolated porcine granulosa and thecal cells were grown on both sides of the amnion and co-cultured with or without RU486 and ZK98, 734. After 48 h incubation, the progesterone receptor (PR) and estrogen receptor (ER) of both cells were detected by flow cytometry. Progesterone and estradiol concentrations in the media were measured by radioimmunoassay. The results showed that antiprogestins increased PR contents in both cells; no significant change was found for ER. At the same time the progesterone and estradiol production by granulosa cells was inhibited; the progesterone production by thecal cells was reduced also. These data suggest that progesterone regulates progesterone synthesis. This autocrine/paracrine action may be the approach through which progesterone controls PR upregulation. It could be one mechanism for the inhibition of follicle development and steroidogenic function by antiprogestins.
Asunto(s)
Estrenos/farmacología , Antagonistas de Hormonas/farmacología , Mifepristona/farmacología , Ovario/fisiología , Receptores de Estrógenos/fisiología , Receptores de Progesterona/fisiología , Amnios/fisiología , Animales , Estradiol/análisis , Femenino , Citometría de Flujo , Células de la Granulosa/efectos de los fármacos , Células de la Granulosa/fisiología , Humanos , Ovario/citología , Ovario/efectos de los fármacos , Progesterona/análisis , Radioinmunoensayo , Receptores de Estrógenos/efectos de los fármacos , Receptores de Progesterona/efectos de los fármacos , Porcinos , Células Tecales/efectos de los fármacos , Células Tecales/fisiología , Regulación hacia ArribaRESUMEN
Defensins are endogenous antimicrobial peptides. We demonstrated recently the existence of RK-1, a defensin-like peptide in rabbit kidneys. Defining the renal-defensin system is an essential step toward understanding how the kidney responds to infection. Here, using a combination of high-performance liquid chromatography, matrix-assisted laser desorption time-of-flight mass spectrometry, amino acid compositional analysis, and microsequence analysis, we show that the rabbit kidney contains a novel alpha-defensin-like peptide, RK-2. This work establishes the existence of a new subfamily of alpha-defensins in the kidney.
Asunto(s)
Antiinfecciosos/metabolismo , Proteínas Sanguíneas/fisiología , Inmunidad/fisiología , Riñón/metabolismo , Secuencia de Aminoácidos , Aminoácidos/análisis , Animales , Defensinas , Datos de Secuencia Molecular , Conejos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
45 acne patients and 38 healthy subjects were assayed for serum testosterone and estradiol levels by RIA. The results showed that serum testosterone levels of male patients were normal, but serum estradiol levels were significantly higher (40%) than in healthy males. In female patients, estradiol levels were normal, but testosterone levels were significantly higher (47%) than in healthy females. Of the 18 female patients, 16 had various degrees of menstrual dysfunction, and some even had slight hirsutism. Therefore, male acne patients should not be treated with estrogen, and in female acne patients with ovarian dysfunction, artificial menstrual cycle therapy is recommended.
