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Objective To explore the therapeutic effect of intravenous transplantation of human amniotic mesenchymal stem cells (hAMSCs) on protection of motor behaviors in hSOD1-G93A mouse models of amyotrophic lateral sclerosis (ALS).Methods Amnion membranes were obtained from placentas delivered by healthy mother donors.The hAMSCs were gradually isolated and purified from amnion membranes using tissue culture method.Immunophenotypes of the isolated hAMSCs were analyzed using fluorescence activated cell sorter (FACS).Transgenic mice harboring a high copy number of hSOD1-G93A (B6SJL-TgN [SOD1-G93A] 1Gur) transgene were used in this study.Hemizygous transgenic progenies were maintained by mating the transgenic males with F1 hybrid wild-type (WT)females.The progenies were genotyped by polymerase chain reaction (PCR) using genomic DNA isolated from mouse tail after birth.The study included hSOD1-G93A mice transplanted with hAMSCs,PBS-injected transgenic mice,and normal WT mice (n=12).The hAMSCs were administered intravenously in jugular vein of the mice under anesthesia.The cells (1 ×106) in 200 μL PBS were delivered over 10 min.Animals received cells or PBS at 12,14,and 16 weeks old,respectively.The disease onset and progression of ALS mice models were monitored using rotarod performance test,PaGE test,and CatWalk gait analysis since 8 weeks old every week.Results (1) The immunophenotype of the isolated hAMSCs was conformed using FACS.These cells were positive for CD29,CD44,CD73,CD90,and CD166,but negative for CD14,CD34,CD45,CD123,and human leukocyte (site) DR antigen.Interestingly,stage specific embryo surface antigen 4 and octuber binding transcription factor 4 were detected in hAMSCs.(2) ALS mice in the hAMSCs transplantation group had significantly improved motor functions than those in the PBS treatment group:motor performance on the rotarod test (from 14 to 18 weeks old),PaGE test (from 15 to 18 weeks old) and CatWalk gait analysis (from 15 to 19weeks old) in hAMSCs-injected ALS mice was significantly improved as compared with that in the PBS treatment group (P＜0.05).Conclusions The multiple transplantation of hAMSCs by intravenous delivery can bring amelioration of the disease phenotype,as evidenced by improved motor function in hSOD1-G93A mouse models.The hAMSCs transplantation can be considered as a promising cellular treatment for ALS.
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Objective To investigate the clinical efficacy of thumb-tack needle therapy for the early and middle stages of senile knee osteoarthritis.Method Sixty patients were allocated, using a random number table, to observation and control groups, 30 cases each.Thecontrol group took meloxicam tablets7.5 mg once daily and the observation group received thumb-tack needle therapy8 hrs once daily inaddition.Liangqiu(ST34), Xiyangguan(GB33), Neixiyan(Ex-LE4), Dubi(ST35), Yinlingquan(SP9),Yanglingquan(GB34)and Ashipoint were selected forthumb-tack needle therapy.Both groups were treated five times a week for two consecutive weeks.The WOMAC score and the Lysholm score were recorded before and after treatment.Theclinical therapeutic effects were evaluated.Result The total efficacy rate was 93.3% inthe observation group and 80.0% in the control group.It was higher inthe observation group thanin the control group and there was a statistically significant difference (P<0.05). The WOMAC score decreased and the Lysholm score increased in both groups after treatment; there were statistically significant pre-/post-treatment differences (P<0.05).after treatment, the WOMAC score and the Lysholm score improved more inthe observation group than in the control group; there were statistically significant differences (P<0.05).Conclusion Thumb-tack needle therapyhas a definite effect on the early and middle stages of senile knee osteoarthritis.It can relieve the pain and improveknee functionin the patients.
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ObjectiveTo investigate the status of benefit finding in diabetic patients,and mediating effect of resilience between family support and benefit finding in diabetic patients.MethodsFive hundred and thirteen diabetic patients from a hospital of Harbin were recruited as investigative subjects from September 2015 to June 2016.The resilience scale,family adaptability,cohesion scale and benefit finding scale were used in the study.ResultsThe scores of benefit finding,resilience and family adaptability and cohesion were 55.67±12.87,70.65±10.39 and 104.25±15.38,separately.The total score of family adaptability and cohesion was positively related to the total score of benefit finding(r=0.389,P<0.05).The total score of resilience was positively related to the total score of benefit finding(r=0.155,P<0.05).The total score of family adaptability and cohesion was positively related to the total score of resilience(r=0.112,P<0.05).Benefit finding as the dependent variable,the results of hierarchical regression analysis indicated that family adaptability and cohesion (β=0.382,P<0.01),resilience(β=0.098,P<0.01)entered the regression equation.There was a partial mediating effect of resilience between family support and benefit finding in diabetic patients.The mediating effect was 0.011,accounted for 2.8% of the total effect.The resilience was the important protective factor for benefit finding.ConclusionFamily support has a direct influence on benefit finding and an indirect effect mediated by resilience on benefit finding.
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Objective To investigate the clinical efficacy of ear acupoint thumbtack needle embedding in treating senile primary insomnia.Method Seventy patients were allocated, using a random number table, to observation and control groups, 35 cases each. The observation group received ear acupoint thumbtack needle embedding with alternate selections of left and right ear acupoints and the control group took estazolam tablets, 1 mg once before bedtime. Both groups were treated once every other day, for 30 days. The Pittsburgh Sleep Quality Index (PSQI) score and the Insomnia Severity Index (ISI) score were recorded before and after treatment. The clinical therapeutic effects were evaluated.Result The total efficacy rate was 91.4% in the observation group and 82.9% in the control group. It was higher in the observation group than in the control group; there was a statistically significant difference (P<0.05). In the observation group, the sleep quality, sleep efficiency and daytime dysfunction scores and the total score were lower after treatment than before (P<0.05). In the control group, the sleep duration and sleep efficiency scores and the total score were lower after treatment than before (P<0.05). After treatment, the sleep disturbance and daytime dysfunction scores and the total score were lower in the observation group than in the control group (P<0.05) and the sleep duration score was lower in the control group than in the observation group (P<0.05). In both groups, the ISI score was lower after treatment than before (P<0.05). It decreased more significantly in the observation group than in the control group; therewas a statistically significant difference (P<0.05).Conclusion Ear acupoint thumbtack needle embedding has a definite therapeutic effect on senile primary insomnia. It can reduce sleep disturbances, improve daytime function and decrease insomnia severity in the patients.
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Objective To investigate the role of hysteroscopy combined with laparoscopy in treatment of infertility.Methods 102 patients with infertility treated by hysteroscopy combined with laparoscopy were retrospectively analyzed.Results Of 102 cases of infertility,there were 57 cases with Pelvic adhesions and tubal obstruction,24 cases with endometriosis,12 cases with polycystic ovary syndrome,4 cases with endometrial polyp,3 cases with hysteromyoma and 2 cases with uterus septum.The first three cases were chronic pelvic inflammatory with salpingemphraxis ( 55.88% ), endometriosis Ⅰ -Ⅱ (23.53% ) and polycystic ovary syndrome (11.76% ).The postoperative pregnancy rate was 41.18%, 3 patients with ectopic pregnancy (7.14%) and 4 spontaneous abortion (9.52%).Conclusion Laparoscopy with hysteroscopy played an important role in treatment of infertility.It was an effective method and worthy to be widely used.
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Objective To explore the effects of air pollution on daily outpatient vivits of hospital. Methods Correlation analysis was carried out based on data of daily outpatient visits of Taiyuan Central Hospital(TCH) and monitoring data on the concentration of TSP and SO2 in air and temeprature and humidity area of Xinghualing district where TCH was located by the time series analysis. Results The concentiations of TSP and SO2 in air exceeded the corresponding standards recommended by WHO. SO2 was the statistically significant forecasting factor for the daily outpatient visits of the department of respiratory disease, emergency and piediatrics. TSP was the statistically singnificant forecasting factor for the daily outpatient visits of department of cardiovascular disease and other departments. Conclusion Severe air pollution by TSP and SO2 effected the dialy outpatient visits in a certain degree.
