Singlet oxygen induces cell wall thickening and stomatal density reducing by transcriptome reprogramming.

Singlet oxygen (1O2) has a very short half-life of 10-5 s; however, it is a strong oxidant that causes growth arrest and necrotic lesions on plants. Its signaling pathway remains largely unknown. The Arabidopsis flu (fluorescent) mutant accumulates a high level of 1O2 and shows drastic changes in nuclear gene expression. Only two plastid proteins, EX1 (executer 1) and EX2 (executer 2), have been identified in the singlet oxygen signaling. Here, we found that the transcription factor abscisic acid insensitive 4 (ABI4) binds the promoters of genes responsive to 1O2-signals. Inactivation of the ABI4 protein in the flu/abi4 double mutant was sufficient to compromise the changes of almost all 1O2-responsive-genes and rescued the lethal phenotype of flu grown under light/dark cycles, similar to the flu/ex1/ex2 triple mutant. In addition to cell death, we reported for the first time that 1O2 also induces cell wall thickening and stomatal development defect. Contrastingly, no apparent growth arrest was observed for the flu mutant under normal light/dim light cycles, but the cell wall thickening (doubled) and stomatal density reduction (by two-thirds) still occurred. These results offer a new idea for breeding stress tolerant plants.

Degradation of dimethyl phthalate through Fe(II)/peroxymonosulphate heightened by fulvic acid: efficiency and possible mechanism.

Ferrous iron (Fe(II)) reacts with peroxymonosulphate (PMS) to form active oxidants that can degrade refractory organic pollutants. However, the conversion rate of Fe(III) to Fe(II) is slow, which limits its actual application. In the study, the effect of fulvic acid (FA) on the degradation of dimethyl phthalate (DMP) by Fe(II)/PMS was investigated. Moreover, the degradation process of DMP was predicted by the preliminary identification of active free radicals and intermediates. As expected, FA gave rise to a higher concentration of Fe(II) than that in Fe(II)/PMS to enhance the removal of DMP in Fe(II)/PMS system. The precipitate, involved in FA and iron, was an important composite to promote the degradation of DMP in the system. Also, the response surface methodology (RSM) was applied to model and optimize the degradation conditions of DMP. The highest removal efficiency (85.70%) was obtained at pH = 3.86, [PMS] = 0.96 mM, [FA] = 11.44 mg/L and [DMP] = 5 µM. The results of free radical quenching experiments and EPR showed that •OH and SO4•- were the main active radicals in this system. The degradation intermediates of DMP were monomethyl phthalate (MMP), phthalic acid and benzoic acid. Discoveries of this study had raised the current understanding of the application of FA keeping the cycles of Fe(II)/Fe(III) for peroxymonosulphate activation, which could afford valuable information for the degradation of organic pollutants by FA/Fe(II)/PMS.

Modification of plasma membrane H+-ATPase in Masson pine (Pinus massoniana Lamb.) seedling roots adapting to acid deposition.

To understand the regulation of roots plasma membrane H+-ATPase in Masson pine responding to acid deposition, the changes in biomass, plant morphology, intracellular H+, enzyme activity and H+-ATPase genes expression in Masson pine seedlings exposed to simulated acid rain (SAR, pH 5.6 and 4.6) with and without vanadate were studied. Simulated acid rain exposure for 60 days increased the intracellular H+ in pine roots whether added with 0.1 mM Na3VO4 or not. The growth of seedlings treated with SAR was maintained well, even the primary lateral root length, root dry weight and number of root tips in seedlings exposed to SAR at pH 4.6 were higher than that of the control (pH 6.6). However, the addition of vanadate resulted in severe growth inhibition and obvious decline in morphological parameters. Similarly, ATP hydrolytic activity and H+ transport activity of roots plasma membrane H+-ATPase, both were stimulated by SAR whereas they were inhibited by vanadate, and the highest activity stimulation was observed in pine roots subjected to SAR at pH 4.6. In addition, SAR also induced the expression of the investigated H+-ATPase subunits (atpB, atpE, atpF, atpH and atpI). Therefore, the roots plasma membrane H+-ATPase is instrumental in the growth of Masson pine seedlings adapting to acid rain by a manner of pumping more protons across the membrane through enhancing its activity, and which involves the upregulated gene expression of roots H+-ATPase subunits at transcriptional level.

Oxidative degradation/mineralization of dimethyl phthalate (DMP) from plastic industrial wastewater using ferrate(VI)/TiO2 under ultraviolet irradiation.

A novel ferrate(VI)/titanium dioxide/ultraviolet [Fe(VI)/TiO2/UV] system was successfully established for the photocatalytic oxidation of dimethyl phthalate (DMP). This system demonstrated a higher removal efficiency of DMP (95.2%) than the conventional TiO2/UV and Fe(VI) alone systems (51.8% and 23.5%, respectively) and produced obvious synergistic effects. Response surface methodology (RSM), based on a three level, three independent variables design, was conducted through Design Expert 8.0.6 program, and a second-order polynomial model (R2 = 0.998) was developed to quantitatively describe the photocatalysis of TiO2 combined with Fe(VI) oxidation under ultraviolet irradiation. The fresh TiO2 and photochemical reacted Fe(VI)/TiO2 were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and element dispersive spectrum (EDS), which indicated that Fe(VI) was imprinted into the TiO2, and the surface adsorbed Fe-O-(organic) materials inhibited DMP degradation. This photocatalytic oxidant showed high activity and stability after nine cycles without loss of its effectiveness (counting from the second cycle). The intermediates/products of DMP were analyzed by gas chromatography-mass spectrometry. The proposed pathway for DMP degradation involved one electron transfer of hydroxyl radical and breaking of the ester bond and benzene ring. The mineralization efficiencies of DMP in actual industrial wastewater and simulated water were 87.1% and 95.2%, respectively, suggesting practical field applications. A ecotoxicity test (17.3% inhibition on bioluminescence) in treating actual industrial wastewater containing DMP implied that the proposed Fe(VI)/TiO2/UV had a potential for industrial water treatment.