RESUMEN
Long-term therapeutic hyperoxia may exert serious toxic effects on intestinal epithelial cells in vitro and in vivo. The aim of the present study was to investigate the cause of this intestinal injury under conditions of hyperoxia. Caco-2 cells were treated with different concentrations of hydrogen peroxide (H2O2) and 85% hyperoxia for 24 h. higher rates of injury of Caco-2 cells were observed in the hyperoxia and H2O2 groups compared with the control group. The reactive oxygen species (ROS) level of the hyperoxia group was significantly higher compared with that of the 400 µM H2O2 group. The protein and gene levels of RelA, RelB, hypoxiainducible factor-1α, tumor necrosis factor-α and apoptosis signalregulating kinase 1 were significantly higher in the hyperoxia and H2O2 groups compared with those in the control group. In conclusion, during hyperoxia, intestinal epithelial cells were destroyed and the levels of ROS were increased. Therefore, ROS may play an important role in intestinal injury in a hyperoxic environment.
Asunto(s)
Hiperoxia/fisiopatología , Mucosa Intestinal/metabolismo , Necrosis/patología , Especies Reactivas de Oxígeno/toxicidad , Animales , Apoptosis/efectos de los fármacos , Apoptosis/genética , Células CACO-2 , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Células Epiteliales/patología , Regulación de la Expresión Génica/genética , Humanos , Peróxido de Hidrógeno/toxicidad , Hiperoxia/inducido químicamente , Hiperoxia/genética , Hiperoxia/metabolismo , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , Mucosa Intestinal/lesiones , Mucosa Intestinal/patología , MAP Quinasa Quinasa Quinasa 5/genética , FN-kappa B/genética , Necrosis/inducido químicamente , Necrosis/metabolismo , Oxígeno/metabolismo , Oxígeno/toxicidad , Especies Reactivas de Oxígeno/metabolismo , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Redox imbalance is established in various human diseases. Treatment of intestinal epithelial cells with hyperoxia for a prolonged period of time may cause serious effects on redox balance. Secretory component (SC) protein is secreted by intestinal epithelial cells, and has a vital role in mucosal immune systems and intestinal defense. The present study aimed to investigate the influence of reactive oxygen species (ROS) on intestinal epithelial cells and intestinal epithelial SC protein under hyperoxic conditions. Caco-2 cells were treated with increasing concentrations of hydrogen peroxide (H2O2) or 85% O2 (hyperoxia) for 24 h. Flow cytometry, immunohistochemistry staining, western blot analysis and reverse transcription-quantitative polymerase chain reaction were performed to detect the expression levels of SC protein. Significantly increased apoptosis and mortality rates were observed in hyperoxia- and H2O2-treated Caco-2 cells, as compared with the untreated control cells (P<0.05). Protein and mRNA expression levels of SC were significantly increased in hyperoxia- and H2O2-treated groups, as compared with the control group (P<0.05). During hyperoxia, intestinal epithelial cells were destroyed and ROS levels increased. Therefore, the results of the present study suggested that ROS might have an important role in intestinal injury in hyperoxic environments.
