Asunto(s)
Neoplasias de la Mama , beta Catenina , Apoptosis , Neoplasias de la Mama/genética , Proteína C-Reactiva , Ciclo Celular , División Celular , Línea Celular Tumoral , Proliferación Celular , Regulación hacia Abajo , Femenino , Humanos , Proteínas del Tejido Nervioso , Vía de Señalización Wnt , beta Catenina/genética , beta Catenina/metabolismoRESUMEN
OBJECTIVE: This study is aimed at analysing the endogenous metabolites profiling of patients with diabetic osteoporosis, so as to provide the reference for pathogenesis research of diabetic osteoporosis. PATIENTS AND METHODS: The 1H-NMR metabolomics technology, combined with pattern recognition analysis and SIMCA-P 12.0 statistical analysis, were employed to identify the metabolites differences between diabetic patients with disordered bone metabolism (research group) and healthy volunteers (normal group) in this study. RESULTS: Compared with normal group, the results show that in research group, the levels of O-acetyl glycoprotein, proline, 1-methyl histidine, tricarboxylic acid cycle (TCA cycle) product (citric acid and α-ketoglutaric acid) decline, while the levels of branched chain amino acids (leucine, isoleucine, valine), glucose, choline, creatine, inositol, glutamine, aspartic acid, alanine, glycine, and citrulline increase. CONCLUSIONS: There are disordered metabolic pathways and imbalanced bone synthetic materials and regulatory substances in diabetic patients with bone metabolic abnormality. These metabolic abnormalities could be the specific indicators in early diagnosis of diabetic osteoporosis.
Asunto(s)
Enfermedades Óseas Metabólicas/metabolismo , Diabetes Mellitus/metabolismo , Metabolómica , Enfermedades Óseas Metabólicas/sangre , Diabetes Mellitus/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Espectroscopía de Protones por Resonancia MagnéticaRESUMEN
Small intestinal mucosal morphology, lactase activity and lactose hydrolysis were examined by peroral intestinal biopsy and intraluminal perfusion of the small intestine in 28 patients with acute and chronic diarrhea. This study shows that lactase deficiency and mucosal damage are more common in childhood diarrhea, and there are positive correlations among intestinal morphology, lactase activity and lactose hydrolysis. A small bowel biopsy is useful both in establishing the diagnosis and guiding dietary treatment for infants with chronic diarrhea. The pathophysiologic mechanism of diarrhea is also discussed.
