Comparison of Effects of Defferent Operation Ways in Treatment of Pigmented Nevus on Face and Neck / 昆明医科大学学报

Objective Through clinical observation and statistics, to get the best curative effect of surgical operation in treating pigment nevus method and provide clinical guidance. Methods We reviewed of face and neck patients (1100 patients) with pigmented nevus in the department in the department of the dermatology,plastic surgery from January 2013 to October 2015, two different methods was designed on each parts and effect of the treatment, especially satisfaction degree was analyzed by statistical methods.Results In 100 cases of patients, only 20 patients had mild scar hyperplasia at the neck incision and the rest of the patients were satisfactory. For special parts such as mouth,nose and eye around,along thedirection of the muscle, arc and along the direction of dermatoglyph incision was designed respectively, patients obtained with higher postoperative satisfaction (P<0.05) . Conclusion In pigmented nevus of face and neck surgery treatment, surgical incision design requires dynamic and static combining method,incision design is important for the postoperative effect and patients' satisfaction.

Study on lingual mucosa carcinogenesis of C57BL/6 mice induced by 4-nitroquinoline 1-oxide / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aimed to induce carcinogenesis of lingual mucosa in C57BL/6 mice by feeding them 4-nitroquinoline 1-oxide (4NQO) solution.</p><p><b>METHODS</b>A total of 85 C57BL/6 mice were randomly divided into distilled water control group (DD group, n=5), 1,2-propylene glycol control group (PG group, n=5), and experimental group (EP group, n= 75). The mice in the experimental group were medially fed in 15 cages. By contrast, the mice in DD, EP, and PG groups were watered with distilled water, 50 mg.L-1 4NQO solution, and 1,2-propylene glycol solution. The mice in EP group were executed every two weeks from week 0, and the mice in the control groups were sacrificed at the 28th week. The mice were weighed. Mucosal lesions were measured by macroscopic observation and histopathologic detection.</p><p><b>RESULTS</b>One mouse in EP group died of unknown reason. The weight of the mice in EP group presented weight loss compared with the mice in DD and PG groups after the 24th week. Seventy-nine macroscopic lesions were observed in the lingual mucosa, oral floor, and upper palatal and buccal mucosa. A total of 70 macroscopic lesions (88.6%) were located in the lingual mucosa. Mucosal lesions changed from simple hyperplasia to squamous cell carcinomas. Well-differentiated squamous cell carcinomas were observed in all mice of EP group by pathological section at the 28th week. No lesion was found in the mice of DD and PG groups.</p><p><b>CONCLUSION</b>The animal model of lingual squamous cell carcinomas was successfully established. The periods from 12th to 16th week and 20th to 28th week were the ideal times for the research on pathogenesis of early and medial-advanced stage during carcinogenesis of squamous cell carcinomas.</p>

Screening of highly efficient small interfering RNAs against bone morphogenetic protein-7 / 南方医科大学学报

<p><b>OBJECTIVE</b>To obtain highly efficient small interfering RNAs (siRNAs) against bone morphogenetic protein-7 (BMP-7) for future functional analysis of BMP-7 in bone homeostasis.</p><p><b>METHODS</b>We designed 4 independent siRNA sequences against rat BMP-7 and transfected them in rat umbilical vein endothelial cells. After 72 h, we examined BMP-7 mRNA and protein level in the transfected cells using real-time PCR and Western blotting, respectively.</p><p><b>RESULTS</b>All the 4 siRNAs effectively reduced BMP-7 expression in rat umbilical vein endothelial cells, and among them BMP-7-3 siRNA showed the highest efficiency.</p><p><b>CONCLUSION</b>We have obtained an efficient siRNA to knockdown BMP-7 expression in vitro for further investigation of the role of BMP-7 in bone and cartilage formation.</p>

A study on puncturing the temporomandibular joint cavity of rabbits and withdrawing synovia / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to study the methods of how to puncture the temporomandibular joint (TMJ) cavity of rabbits and get the synovia.</p><p><b>METHODS</b>The authors punctured joint cavity and withdrew the synovia from bilateral TMJ cavities of 12 New Zealand rabbits with different methods, such as the traditional puncture method, improved puncture, puncture under X ray, puncture guided by ultrasonography and spiral CT, and withdrawing synovia by micropump.</p><p><b>RESULTS</b>Only one cavity was successfully punctured using the traditional puncture method. A total of 4 cases were successfully punctured using the improved method. X ray and ultrasonography did not locate the TMJ cavity precisely. The spiral CT helped observation and puncture. However, the above methods did not work in collecting synovia. The method of micropump withdrew synovia successfully.</p><p><b>CONCLUSION</b>The improved method and spiral CT are efficient methods to precisely puncture TMJ cavity. The technique of micropump successfully resolves the problem of withdrawing synovia.</p>

An experimental study on gene transfection of human interleukin-1 receptor antagonist gene into chondrocytes of temporomandibular joint / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate methods and expression of the recombinant human interleukin-1 receptor antagonist gene transfected into chondrocytes of temporomandibular joints (TMJ).</p><p><b>METHODS</b>Chondrocytes derived from the TMJ of 5-7 months old human fetus were transfected by recombinant human interleukin-1 receptor antagonist gene via cationic liposome (Lipefectine) as a medium. The expressing level of hIL-1ra protein was detected using immunocytochemistry and enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The positive results of the immunocytochemistry were found. The positive expression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection (P < 0.01).</p><p><b>CONCLUSION</b>This feasible method provides experimented evidence for the future gene therapy of temporomandibular joint osteoarthrosis.</p>

The Application of Decompression and Suction Drainage in the Treatment of Mandibular Cystic Lesions / 昆明医科大学学报

Objective To evaluate the effect of decompression suction drainage treated mandibular cystic lesions,and compared with the othertreatments such as surgery,decompression,and suction drainage.In order to explore its value in the field of mandible conservative treatment.Methods 36 cases of mandibular cystic lesions were performed decompression and suction drainage.Compared the cystic size before and after the treatment to evaluate the clinical effect.Results According the pathology,29 cases are cyst and 7 cases are mural-ameloblastoma.28 cases which X-ray show a remarkable lessen,6 cases have no change and 2 cases deteriorated.Conclusion decompression suction drainage is one of the ideal treatment for mandibular cystic lesions.