RESUMEN
Automated inspection has proven to be the most effective approach to maintaining quality in industrial-scale manufacturing. This study employed the eye-in-hand architecture in conjunction with deep learning and convolutional neural networks to automate the detection of defects in forged aluminum rims for electric vehicles. RobotStudio software was used to simulate the environment and path trajectory for a camera installed on an ABB robot arm to capture 3D images of the rims. Four types of surface defects were examined: (1) dirt spots, (2) paint stains, (3) scratches, and (4) dents. Generative adversarial network (GAN) and deep convolutional generative adversarial networks (DCGAN) were used to generate additional images to expand the depth of the training dataset. We also developed a graphical user interface and software system to mark patterns associated with defects in the images. The defect detection algorithm based on YOLO algorithms made it possible to obtain results more quickly and with higher mean average precision (mAP) than that of existing methods. Experiment results demonstrated the accuracy and efficiency of the proposed system. Our developed system has been shown to be a helpful rim defective detection system for industrial applications.
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Aprendizaje Profundo , Robótica , Algoritmos , Redes Neurales de la ComputaciónRESUMEN
<p><b>OBJECTIVE</b>To investigate the expression of ErbB-3 binding protein-1 (EBP-1), matrix metalloproteinase 9 (MMP-9) and E-cadherin (E-cad) in adenoid cystic carcinoma and their correlation.</p><p><b>METHODS</b>Immunohistochemistry(PV6000 method) was used to detect EBP-1, MMP-9 and E-cad expression in 66 cases of adenoid cystic carcinoma tissues and matched para-cancerous normal tissues. In this study all cases were successfully followed up.</p><p><b>RESULTS</b>The positive expression rate of EBP-1 in adenoid cystic carcinoma tissues was 85%. EBP-1 expression was significantly correlated to pathological pattern and clinical stage (P < 0.05), but not to gender and age. In addition, there was a negative correlation between EBP-1 and E-cad expression, and positive correlation between EBP-1 and MMP-9.</p><p><b>CONCLUSIONS</b>EBP-1 and its correlation with MMP-9 and E-cad may be used as useful indicators for clinical assessment of tumor biological behavior and prognosis in patients with adenoid cystic carcinoma.</p>
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Femenino , Humanos , Masculino , Persona de Mediana Edad , Proteínas Adaptadoras Transductoras de Señales , Metabolismo , Biomarcadores de Tumor , Metabolismo , Cadherinas , Metabolismo , Carcinoma Adenoide Quístico , Metabolismo , Patología , Estudios de Seguimiento , Neoplasias Pulmonares , Metástasis Linfática , Metaloproteinasa 9 de la Matriz , Metabolismo , Invasividad Neoplásica , Estadificación de Neoplasias , Proteínas de Unión al ARN , Metabolismo , Neoplasias de las Glándulas Salivales , Metabolismo , PatologíaRESUMEN
<p><b>OBJECTIVE</b>To investigate the role of transcription factor special AT-rich binding protein 2 (SATB2) in the osteoblasts differentiation of bone marrow stromal cells (BMSC) in vitro.</p><p><b>METHODS</b>Rats bone marrow stromal cells were isolated by Percoll sedimentation and the cells were placed and allowed to attach for three times. After passages, expression plasmid pBABE-hygro-satb2 was constructed, then transfected into BMSC. BMSCs were inoculated in conditioned medium and osteogenic factors were detected by western blotting and reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The morphological observation of BMSC showed either spindle or polygonal pattern. The cellular phenotypic marker of the third passage was CD29 positive and CD34 negative. The growth curve possessed "S" pattern. The intensity of calfilication in BMSC was higher in SATB2 transfection group (IA value 125974 ± 241) than that in the control groups (IA value 178486 ± 406). Moreover, cell migration rate increased in SATB2 transfection group [width of scratch (0.72 ± 0.01) mm] compared with control group [width of scratch (0.83 ± 0.03) mm]. In addition, the mRNA expression of osteogenic factors runt-related transcription factor 2, Osterix, activating transcription factor 4, integrin-binding sialoprotein were upregulated.</p><p><b>CONCLUSIONS</b>Cells cultured with this method have general biological characteristics and osteogenic differentiation potential in vitro. SATB2 can promote osteoblasts differentiation of BMSC.</p>
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Animales , Masculino , Ratas , Factor de Transcripción Activador 4 , Metabolismo , Células de la Médula Ósea , Metabolismo , Patología , Diferenciación Celular , Movimiento Celular , Células Cultivadas , Subunidad alfa 1 del Factor de Unión al Sitio Principal , Metabolismo , Sialoproteína de Unión a Integrina , Metabolismo , Proteínas de Unión a la Región de Fijación a la Matriz , Genética , Metabolismo , Osteoblastos , Biología Celular , Osteogénesis , Plásmidos , ARN Mensajero , Metabolismo , Ratas Sprague-Dawley , Células del Estroma , Metabolismo , Patología , Antígenos Thy-1 , Metabolismo , Factores de Transcripción , Genética , Metabolismo , TransfecciónRESUMEN
Due to an increased risk of infection, dental implant in organ transplantation patients has long been considered questionable, particularly when the restoration is complicated. Five-year follow-up data of a 45-year-old liver transplant recipient with long-term immunosuppressive therapy was reported. One year after liver transplantation, 11 Brånemark implants were inserted in the maxilla and mandible, using minimally invasive surgery. Oral clinical parameters included peri-implant bone absorption, probing depth, and implant mobility. The measured fifth-year parameters were within normal ranges indicating a stable osseointegration with moderate vertical bone loss. This case report suggests that immunocompromised patients can be successfully rehabilitated with dental implants through careful examination, suitable antibiotic administration, and minimally invasive dental implant procedure.
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Humanos , Masculino , Persona de Mediana Edad , Implantes Dentales , Inmunosupresores , Usos Terapéuticos , Trasplante de Hígado , Alergia e InmunologíaRESUMEN
<p><b>OBJECTIVE</b>To study the p53/p21 fusion gene as a potential fusion gene for the gene therapy of human oral squamous cell carcinoma.</p><p><b>METHODS</b>p21 cDNA was obtained from normal human embryonic lung cells by RT-PCR, fusing with p53 gene. The recombinant plasmid pcDNA-p53/p21 was constructed by inserting the p53/p21 fusion gene into eukaryotic expression vector pcDNA3.1 and subsequently transfected into human oral squamous cell carcinoma cell line (Tca8113) with lipofectamine. RT-PCR and Western blot were used to demonstrate the expression of p53/p21 fusion gene. Using clonal formation experiment and (3)H-TdR incorporation assay were used to evaluate the clonal formation and proliferation ability of Tca8113 cells.</p><p><b>RESULTS</b>It was observed that p53/p21 fusion gene could inhibit clonal formation and proliferation of human oral carcinoma. RT-PCR and Western blot demonstrated that it was the expression of exogenous p53/p21 fusion gene that led to the above results.</p><p><b>CONCLUSIONS</b>Transfection of p53/p21 fusion gene to Tca8113 cells could inhibit the tumor cell proliferation and clone formation in vitro, and make itself a potential fusion gene for the gene therapy of human oral squamous cell carcinoma.</p>