RESUMEN
Colon cancer is one of the most common digestive tract malignancies, having the second highest mortality rate among all tumors, with a five-year survival of advanced patients of only 10%. Efficient, targeted drugs are still lacking in treating colon cancer, so it is urgent to explore novel druggable targets. Here, we demonstrated that annexin A1 (ANXA1) was overexpressed in tumors of 50% of colon cancer patients, and ANXA1 overexpression was significantly negatively correlated with the poor prognosis of colon cancer. ANXA1 promoted the abnormal proliferation of colon cancer cells in vitro and in vivo by regulating the cell cycle, while the knockdown of ANXA1 almost totally inhibited the growth of colon cancer cells in vivo. Furthermore, ANXA1 antagonized the autophagic death of honokiol in colon cancer cells via stabilizing mitochondrial reactive oxygen species. Based on these results, we speculated that ANXA1 might be a druggable target to control colon cancer and overcome drug resistance.
RESUMEN
In this study, a highly sensitive and reliable method for the determination of 20 polyfluoroalkane substances (PFASs) in milk was established using the QuEChERS approach and an online interference trapping LC-MS/MS analysis. By a calibration with stable-isotope-labeled internal standards, we showed that the method displayed excellent linear dynamic ranges for 20 PFASs (correlation coefficients ≥0.997). The LOQs for the two types of PFASs, perfluorinated carboxylic acids (PFCAs) and perfluorinated sulfonic acids (PFSAs), were 0.010 and 0.050 µg/L, respectively. At the three spiking levels, the average recoveries for PFCAs ranged from 78.5 to 111% with the RSD (n = 6) within 1.20-13.1%, and those for PFSAs ranged from 72.8 to 105% with the RSD (n = 6) within 3.53-14.9%. By the developed method, 16 PFASs were found to be positive in 46 milk samples, and the levels for the PFASs with longer chains were significantly higher than those reported from other known regions.