RESUMEN
CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element-mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.
Asunto(s)
Segregación Cromosómica/genética , Proteínas de Drosophila , Cinetocoros/metabolismo , Metafase/genética , Proteínas Asociadas a Microtúbulos/genética , Animales , Ciclo Celular/genética , Células Cultivadas , Centrómero/metabolismo , Proteínas Cromosómicas no Histona/genética , Clonación Molecular , Drosophila , Femenino , Eliminación de Gen , Genes Letales , Mutación de Línea Germinal , Proteínas Asociadas a Microtúbulos/metabolismo , Mitosis/genética , Proteínas Motoras Moleculares/genética , Proteínas Motoras Moleculares/metabolismo , Datos de Secuencia Molecular , Mutagénesis Insercional , Homología de Secuencia de AminoácidoRESUMEN
The role of cytoskeletal elements in the cellularization of syncytial Drosophila embryos is becoming evident; however, the distribution and role of organelles such as the Golgi complex, essential for membrane biogenesis, remain unknown. We have cloned a Golgi-membrane-associated polypeptide, beta-COP, from Drosophila. Immunocytochemical studies of syncytial Drosophila embryos with anti-Drosophila beta-COP antibody reveal that Golgi membranes are spatially segregated from the rapidly dividing nuclei. In early embryos, the Golgi membranes are located in the embryonic cortex and nuclei are confined to the core. This distribution of Golgi membranes may serve in preparation of the embryonic cortex for the accommodation of nuclei upon their eventual migration to the cortex and in biogenesis of the excessive plasma membrane needed for cellularization of syncytial embryos.
Asunto(s)
Drosophila melanogaster/embriología , Aparato de Golgi/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , División Celular , Núcleo Celular/metabolismo , Clonación Molecular , Proteína Coatómero , ADN Complementario/genética , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Técnica del Anticuerpo Fluorescente , Células Gigantes/metabolismo , Proteínas Asociadas a Microtúbulos/genética , Datos de Secuencia Molecular , Ratas , Proteínas Recombinantes de Fusión/genética , Homología de Secuencia de AminoácidoAsunto(s)
Membrana Celular/metabolismo , Proteínas de Unión al GTP/metabolismo , Membranas Intracelulares/metabolismo , Fosfolipasa D/metabolismo , Transducción de Señal , Factores de Ribosilacion-ADP , Animales , Proteínas Portadoras/metabolismo , Genes Letales , Aparato de Golgi/metabolismo , Humanos , Orgánulos/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismoRESUMEN
We have recently shown that ilimaquinone (IQ) causes the breakdown of Golgi membranes into small vesicles (VGMs for vesiculated Golgi membranes) and inhibits vesicular protein transport between successive Golgi cisternae (Takizawa et al., 1993). While other intracellular organelles, intermediate filaments, and actin filaments are not affected, we have found that cytoplasmic microtubules are depolymerized by IQ treatment of NRK cells. We provide evidence that IQ breaks down Golgi membranes regardless of the state of cytoplasmic microtubules. This is evident from our findings that Golgi membranes break down with IQ treatment in the presence of taxol stabilized microtubules. Moreover, in cells where the microtubules are first depolymerized by microtubule disrupting agents which cause the Golgi stacks to separate from one another and scatter throughout the cytoplasm, treatment with IQ causes further breakdown of these Golgi stacks into VGMs. Thus, IQ breaks down Golgi membranes independently of its effect on cytoplasmic microtubules. Upon removal of IQ from NRK cells, both microtubules and Golgi membranes reassemble. The reassembly of Golgi membranes, however, takes place in two sequential steps: the first is a microtubule independent process in which the VGMs fuse together to form stacks of Golgi cisternae. This step is followed by a microtubule-dependent process by which the Golgi stacks are carried to their perinuclear location in the cell. In addition, we have found that IQ has no effect on the structural organization of Golgi membranes at 16 degrees C. However, VGMs generated by IQ are capable of fusing and assembling into stacks of Golgi cisternae at 16 degrees C. This is in contrast to the cells recovering from BFA treatment where, after removal of BFA at 16 degrees C, resident Golgi enzymes fail to exit the ER, a process presumed to require the formation of vesicles. We propose that at 16 degrees C there may be general inhibition in the process of vesicle formation, whereas the process of vesicle fusion is not affected.
Asunto(s)
Aparato de Golgi/fisiología , Membranas Intracelulares/fisiología , Microtúbulos/fisiología , Animales , Antibacterianos/farmacología , Brefeldino A , Línea Celular , Ciclopentanos/farmacología , Citoplasma/metabolismo , Citoplasma/fisiología , Citoplasma/ultraestructura , Aparato de Golgi/metabolismo , Aparato de Golgi/ultraestructura , Membranas Intracelulares/metabolismo , Membranas Intracelulares/ultraestructura , Microscopía Electrónica , Microtúbulos/metabolismo , Microtúbulos/ultraestructura , Paclitaxel/farmacología , Quinonas/farmacología , Ratas , TemperaturaRESUMEN
We have identified a novel natural metabolite, ilimaquinone (IQ), from sea sponges that causes Golgi membranes to break down completely in vivo into small vesicular structures (called vesiculated Golgi membranes [VGMs]). Under these conditions, transport of newly synthesized proteins from endoplasmic reticulum (ER) to the cis-Golgi-derived VGMs is unaffected; however, further transport along the secretory pathway is blocked. Upon removal of the drug, VGMs reassemble rapidly into a Golgi complex, and protein transport is restored. By employing a cell-free system that reconstitutes vesicular transport between successive Golgi cisternae, we provide evidence that the inhibition of protein transport by IQ is specifically due to an inhibition of transport vesicle formation. In addition, like brefeldin A (BFA), IQ treatment prevents the association of beta-COP and ADP-ribosylation factor to the Golgi membranes; however, unlike BFA treatment, there is no retrograde transport of Golgi enzymes into ER.