Deep learning pose detection model for sow locomotion.

Lameness affects animal mobility, causing pain and discomfort. Lameness in early stages often goes undetected due to a lack of observation, precision, and reliability. Automated and non-invasive systems offer precision and detection ease and may improve animal welfare. This study was conducted to create a repository of images and videos of sows with different locomotion scores. Our goal is to develop a computer vision model for automatically identifying specific points on the sow's body. The automatic identification and ability to track specific body areas, will allow us to conduct kinematic studies with the aim of facilitating the detection of lameness using deep learning. The video database was collected on a pig farm with a scenario built to allow filming of sows in locomotion with different lameness scores. Two stereo cameras were used to record 2D videos images. Thirteen locomotion experts assessed the videos using the Locomotion Score System developed by Zinpro Corporation. From this annotated repository, computational models were trained and tested using the open-source deep learning-based animal pose tracking framework SLEAP (Social LEAP Estimates Animal Poses). The top-performing models were constructed using the LEAP architecture to accurately track 6 (lateral view) and 10 (dorsal view) skeleton keypoints. The architecture achieved average precisions values of 0.90 and 0.72, average distances of 6.83 and 11.37 in pixel, and similarities of 0.94 and 0.86 for the lateral and dorsal views, respectively. These computational models are proposed as a Precision Livestock Farming tool and method for identifying and estimating postures in pigs automatically and objectively. The 2D video image repository with different pig locomotion scores can be used as a tool for teaching and research. Based on our skeleton keypoint classification results, an automatic system could be developed. This could contribute to the objective assessment of locomotion scores in sows, improving their welfare.

Lameness in Pregnant Sows Alters Placental Stress Response.

Pregnant sows from commercial pig farms may experience painful states, such as lameness, an essential indicator in assessing sow welfare. We investigated the effect of lameness during the last third of pregnancy on reproductive performance and placental glucocorticoid concentrations in sows. Periodic locomotion assessments were carried out on two commercial pig farms using a validated 0-5 scoring system (from 0: normal locomotion; to 5: a downer animal). Sows from both farms (N = 511) were grouped based on their average locomotion scores. On Farm 1, 30 sows were selected and grouped as either Not Lame (NL = 16; X¯ = 0-1) or Lame (L = 14; X¯ > 1). On Farm 2, 39 sows were selected and grouped as either Not Lame (G1 = 12; X¯ = 0-1), Moderately Lame (G2 = 13; X¯ = 1.1-2), or Severely Lame (G3 = 14; X¯ ≥ 2.1). Reproductive data (gestation length, litter weight, average piglet weight, litter size, and the number of piglets born alive/mummified/stillborn) were recorded on both farms. Moreover, on Farm 2, piglet intrauterine growth restriction score and the number of piglets dead during the first week were also recorded, and placenta samples were collected to determine their cortisol/cortisone concentrations. A linear mixed model was used to analyze the data. The proportion of lameness in pregnant sows (N = 511) was >40%, and the gestation length tended to decrease with the presence of lameness (p < 0.1) in both farms. G2 sows had a higher placental cortisol/cortisone ratio than G1 and G3 sows (p < 0.01). In conclusion, lameness was high in the sows assessed, which may decrease sow gestation length and reduce placental efficiency in protecting the offspring from the sows' stress response.

Asinine milk mitigates stress-mediated immune, cortisol and behavioral responses of piglets to weaning: A study to foster future interventions in humans.

Introduction: The present study assessed whether asinine milk supplementation improved the immune and behavioral responses of piglets during an early life weaning stress event as a model for its future use in humans. Methods: For this, 48 piglets from 4 different litters were used. At 20 days of age, piglets were weighed and allocated with their litter and dam into group pens until 28 days of age. Four piglets from each litter were then randomly assigned to either (1) asinine milk supplementation (n = 16) (2), skimmed cow milk supplementation (n = 16) or (3) no supplementation (n = 16; control group). The supplementations were voluntarily administered for 3 days preweaning and 3 days postweaning using a baby bottle. The effects on the weaning stress response were assessed through salivary cortisol measurements; behavioral tests such as the open field, novel object end elevated plus maze tests; and gene expression of HSD11B1, NR3C1 and IL1B in PBMCs, which was determined by RT-qPCR and normalized to GAPDH and UBB. To test the effect of the supplementations on weight, milk intake, gene expression, and behavior, a randomized block design was used with repeated measurements over time by the PROC MIXED procedure. Results and discussion: The effects on salivary cortisol were determined using the ratio between the morning and afternoon concentrations, considering the time before and after the weaning event. Principal component analysis (PCA) and Fisher's test were performed to evaluate the behavior test data. When comparing salivary cortisol concentrations between the pre- and postweaning periods, there was a difference (p < 0.05) between the supplementation groups in the afternoon period, suggesting that piglets fed asinine milk had lower afternoon cortisol concentrations postweaning than their counterparts. For the behavioral tests, the supplementations had no measurable effects. No difference was between groups pre- and postweaning for the expression of HSD11B2, which codes for an enzyme that breaks down cortisol. However, the expression of NR3C1, which encodes the glucocorticoid receptor, was significantly upregulated in piglets supplemented with cow milk (mean 1.245; p < 0.05). Conclusion: Asinine milk downregulated 1L1B gene expression, which codes for an inflammatory cytokine. In conclusion, these results suggest that supplementation with asinine milk may represent a strategy to diminish the damage associated with an early life event by modulating IL1B expression and reducing salivary cortisol levels in piglets undergoing weaning stress. Further transcriptomic and metabolomic studies may improve our understanding of the molecular pathways that mediate this systemic immune-mediated response.

An enriched maternal environment and stereotypies of sows differentially affect the neuro-epigenome of brain regions related to emotionality in their piglets.

Epigenetic mechanisms are important modulators of neurodevelopmental outcomes in the offspring of animals challenged during pregnancy. Pregnant sows living in a confined environment are challenged with stress and lack of stimulation which may result in the expression of stereotypies (repetitive behaviours without an apparent function). Little attention has been devoted to the postnatal effects of maternal stereotypies in the offspring. We investigated how the environment and stereotypies of pregnant sows affected the neuro-epigenome of their piglets. We focused on the amygdala, frontal cortex, and hippocampus, brain regions related to emotionality, learning, memory, and stress response. Differentially methylated regions (DMRs) were investigated in these brain regions of male piglets born from sows kept in an enriched vs a barren environment. Within the latter group of piglets, we compared the brain methylomes of piglets born from sows expressing stereotypies vs sows not expressing stereotypies. DMRs emerged in each comparison. While the epigenome of the hippocampus and frontal cortex of piglets is mainly affected by the maternal environment, the epigenome of the amygdala is mainly affected by maternal stereotypies. The molecular pathways and mechanisms triggered in the brains of piglets by maternal environment or stereotypies are different, which is reflected on the differential gene function associated to the DMRs found in each piglets' brain region . The present study is the first to investigate the neuro-epigenomic effects of maternal enrichment in pigs' offspring and the first to investigate the neuro-epigenomic effects of maternal stereotypies in the offspring of a mammal.

Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts.

The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain.

Poor welfare compromises testicle physiology in breeding boars.

In commercial pig breeding farms, boars are often exposed to stressful situations, such as confined housing conditions, inadequate environmental temperature, food restriction, lameness, diseases, among other challenges. Confined housing conditions, such as crates, are reported as a major source of stress for pregnant sows, and were banned in the UK and in Europe, however there is limited information about the impact of this housing system for boars. The goal of this study was to investigate the impact of three different housing conditions for boars and the consequence on the testicles. We studied 27 crossbred boars (F1 large white and landrace), housed in crates (n = 9), pens (n = 9), or enriched pens (n = 9), during 10 weeks. We collected data of scrotal superficies mean temperature (SSMT) with a thermal camera; we measured testicular parenchyma perfusion (ultrasound evaluation); and we measured sperm characteristics. We found that boars housed in crates had a higher SSMT (p < 0.05) and higher testicular parenchyma perfusion than boars housed in pens and enriched pens (p = 0.01). Regarding the semen features, we found that boars housed in crates showed more agglutinated semen, and higher values of linear curved linear velocity (VCL) than boars housed in pens and enriched pens, both indicators of reduced fertility. These results indicates that boars housed in pens and in enriched pens showed better indicators of testicular health, better sperm motility features (VCL, p = 0.046), and less agglutinated sperm (p < 0;05) than that observed in boars kept in crates.

Vaginal Microbiota Diversity in Response to Lipopolysaccharide in Gilts Housed Under Three Housing Systems.

The United Kingdom and European Union have banned crates for pregnant sows. However, animals are kept in a restrictive environment for up to four weeks after mating, leading to stress and different responses of the animals' immune system. Here, we used vaginal flushing of gilts to investigate whether housing systems or an experimental inflammatory challenge with lipopolysaccharide (LPS) can modify the gilt vaginal microbiome. Alpha-diversity indices showed differences in the microbiota of gilts housed under different systems (q = 0.04). Shannon alpha-diversity richness was higher in gilts group-housed in pens than in gilts housed in crates (q = 0.035), but not higher than in other groups. The relative abundance of the operational taxonomic unit (OTU) (q < 0.05) revealed specific differences in housing systems before a LPS or saline (SAL control) challenge. We found different abundances in taxa of Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Proteobacteria in gilts housed in the different systems before challenge. After the LPS challenge, significant differences were detected in the relative abundance of OTUs (q < 0.05) for the LPS-challenged group compared with SAL animals for each housing system. The phylum Staphylococcus showed higher abundance among the LPS-challenged gilts than in SAL-challenged animals. Furthermore, Enterobacter was more abundant in the LPS-challenged gilts housed in crates than in SAL-challenged gilts housed in crates. Streptococcus suis, Conchiformibius, Globicatella and Actinobacillus were more abundant in LPS-challenged gilts in indoor group housing than in SAL gilts in the same housing system. Gilts kept outdoors did not show changes in vaginal microbiota after an LPS challenge. Gilts housed in crates showed clinical signs of urogenital infection, whereas gilts housed outdoors and in indoor group housing did not. The relationship between environment, immune response, and microbiota suggested that animals in a poor environments experience difficulties responding to a challenge and their vaginal microbiota is altered as a consequence, with decreased richness of normal vaginal microbiota, and increased opportunistic bacteria. Welfare indicators measured by gilts' responses to housing systems however, do not fully explain mechanisms associated with the unique signature in vaginal microbiota encountered in the different housing systems.

Pain assessment in horses using automatic facial expression recognition through deep learning-based modeling.

The aim of this study was to develop and evaluate a machine vision algorithm to assess the pain level in horses, using an automatic computational classifier based on the Horse Grimace Scale (HGS) and trained by machine learning method. The use of the Horse Grimace Scale is dependent on a human observer, who most of the time does not have availability to evaluate the animal for long periods and must also be well trained in order to apply the evaluation system correctly. In addition, even with adequate training, the presence of an unknown person near an animal in pain can result in behavioral changes, making the evaluation more complex. As a possible solution, the automatic video-imaging system will be able to monitor pain responses in horses more accurately and in real-time, and thus allow an earlier diagnosis and more efficient treatment for the affected animals. This study is based on assessment of facial expressions of 7 horses that underwent castration, collected through a video system positioned on the top of the feeder station, capturing images at 4 distinct timepoints daily for two days before and four days after surgical castration. A labeling process was applied to build a pain facial image database and machine learning methods were used to train the computational pain classifier. The machine vision algorithm was developed through the training of a Convolutional Neural Network (CNN) that resulted in an overall accuracy of 75.8% while classifying pain on three levels: not present, moderately present, and obviously present. While classifying between two categories (pain not present and pain present) the overall accuracy reached 88.3%. Although there are some improvements to be made in order to use the system in a daily routine, the model appears promising and capable of measuring pain on images of horses automatically through facial expressions, collected from video images.

The in-utero experience of piglets born from sows with lameness shapes their life trajectory.

Experiences during gestation can alter the mother's behavior and physiology, thereby potentially affecting the behavioral and physiological development of the offspring. In livestock, one common challenge for pregnant animals is lameness: a multifactorial condition that causes pain, stress, resulting in poor welfare outcomes. Since maternal pain can affect offspring development, we aimed to quantify the behavioral response in 142 piglets born from sows with different degrees of lameness during pregnancy. Gait scores of 22 pregnant group-housed sows were assessed six times at 2-week intervals. Lameness scores varied from 0 (no lameness) to 5 (most severe lameness score). Saliva samples and behavior were assessed in the sows throughout pregnancy. Sows were moved to individual farrowing pens and placental tissue was collected for glucocorticoid assessment. At 28 days of age, piglets were weaned, weighed, and regrouped by body size and sex. Skin lesions were counted for each piglet on days 28, 29, and 30 after birth. During open field and novel object tests on day 30, the vocalization and activity levels were evaluated. Piglet data were grouped by the lameness score of the sows as G1 (without lameness), G2 (moderate lameness), and G3 (severe lameness). Data analysis included ANOVA or Kruskal-Wallis tests and pairwise comparisons which were performed using Tukey and Kramer (Nemenyi) test with Tukey-Dist approximation for independent samples. G2 piglets were heavier than G3 at weaning. G1 piglets had fewer skin lesions at days 28 and 29 than G2 piglets. Moreover, G1 piglets vocalized more than G2 when they were subjected to the combined open field and novel object test. We did not identify differences among sows showing different lameness scores in the concentration of placental or salivary glucocorticoids. Lameness in pregnant sows altered the offspring's weight gain, number of skin lesions and vocalizations, together showing evidence that lameness in sows affect offspring performance and behavior.

Two Hours of Separation Prior to Milking: Is This Strategy Stressful for Jennies and Their Foals?

The goal of this study was to assess whether or not a separation period of 2 h is stressful for jennies and foals, as measured by changes in behaviour, salivary cortisol, and milk production. This study was reviewed and approved by the Committee for the Use and Care of Animals in Research (CEUA) of the School of Veterinary Medicine and Animal Science of the University of São Paulo. Fourteen multiparous Pêga jennies (245 kg average body weight) and their foals were assessed from day 45 to 135 of lactation. Dams and foals were separated for 2 h prior to milking. Behavioural assessments and saliva samples were collected before and after separation, every 15 days, resulting in 14 samples per individual animal. Behavioural states (affiliative and inactivity) and events (agonistic, abnormal, eliminative and vocalisations) of the jennies were observed during 6 min in both periods. Moreover, milk yield was measured. Few significant behavioural and salivary cortisol changes were observed, and milk yield was not affected by cortisol levels in response to the separation. The 2-h separation period, on the basis of the collected variables, did not appear to be stressful for the assessed group of Pêga jennies or foals; however, their ability to adapt to milking routine stress remains to be investigated.

How Epigenetics Can Enhance Pig Welfare?

Epigenetics works as an interface between the individual and its environment to provide phenotypic plasticity to increase individual adaptation capabilities. Recently, a wide variety of epi-genetic findings have indicated evidence for its application in the development of putative epi-biomarkers of stress in farm animals. The purpose of this study was to evaluate previously reported stress epi-biomarkers in swine and encourage researchers to investigate potential paths for the development of a robust molecular tool for animal welfare certification. In this literature review, we report on the scientific concerns in the swine production chain, the management carried out on the farms, and the potential implications of these practices for the animals' welfare and their epigenome. To assess reported epi-biomarkers, we identified, from previous studies, potentially stress-related genes surrounding epi-biomarkers. With those genes, we carried out a functional enrichment analysis of differentially methylated regions (DMRs) of the DNA of swine subjected to different stress-related conditions (e.g., heat stress, intrauterine insult, and sanitary challenges). We identified potential epi-biomarkers for target analysis, which could be added to the current guidelines and certification schemes to guarantee and certify animal welfare on farms. We believe that this technology may have the power to increase consumers' trust in animal welfare.

The development of the AWIN welfare assessment protocol for donkeys / O desenvolvimento do protocolo de avaliação de bem-estar AWIN (Animal Welfare Indicators) para jumentos

The donkey population has increased in the last 10 years, with an estimated 50 million donkeys currently worldwide. Donkey welfare, meanwhile, is an increasing global concern that receives close public scrutiny. However, multiple challenges are surrounding how donkey welfare is assessed and recorded. The Animal Welfare Indicators (AWIN) project is the first project, funded by the European Commission, intended to improve donkey welfare by developing a scientifically sound and practical on-farm welfare assessment protocol. The present study describes the procedure for the development of the AWIN welfare assessment protocol for donkeys: 1) selection of promising welfare indicators; 2) research to cover gaps in knowledge; 3) stakeholder consultation; 4) testing the prototype protocol on-farm. The proposed two-level strategy improved on-farm feasibility, while the AWIN donkey app enables the standardized collection of data with prompt results. Although limitations are linked with a relatively small reference population, the AWIN welfare assessment protocol represents the first scientific and standardized approach to evaluate donkey welfare on-farm.(AU)

Na última década, a população de jumentos vem aumentando; estima-se que existam aproximadamente 50 milhões de em todo o mundo. O bem-estar dos jumentos é uma preocupação global crescente, que recebe um escrutínio público próximo. No entanto, existem vários desafios em torno de como o bem-estar do jumento é avaliado e registrado. O projeto Indicadores de Bem-Estar Animal (AWIN) foi o primeiro projeto, financiado pela Comissão Europeia, destinado a melhorar o bem-estar dos jumentos, desenvolvendo um protocolo de avaliação do bem-estar cientificamente válido e prático na fazenda. O presente estudo descreve o procedimento para o desenvolvimento do protocolo de avaliação de bem-estar AWIN para jumentos: 1) seleção de indicadores promissores de bem-estar; 2) pesquisa para cobrir lacunas no conhecimento; 3) consulta às partes interessadas; 4) testando o protocolo do protótipo em fazendas. A estratégia proposta em dois níveis de avaliação melhorou a viabilidade na fazenda, além disso, o aplicativo AWIN donkey permite coletar dados de maneira padronizada e mostrar resultados rapidamente. Embora a limitação esteja ligada a uma população de referência relativamente pequena, o protocolo de avaliação de bem-estar do AWIN representa a primeira abordagem científica e padronizada para avaliar o bem-estar de jumentos em fazendas.(AU)

The population of donkeys and mules in Brazil according to agricultural censuses from 1960 to 2017 / População de jumentos e muares no Brasil segundo os censos agropecuários de 1960 a 2017

To better understand and manage the population of donkeys and mules, it is essential to know information about its size. In developing countries, these animals are often used as working animals, mainly by poor populations in agricultural and livestock labor, but their use is decreasing in Brazil. Brazilian official data regarding donkeys and mules started to be measured at the agricultural census of 1960 and, from that time, eight agricultural censuses were published. But until the writing of this paper, no scientific paper was dedicated to the analysis of the results of the official agricultural censuses on the population of donkeys and mules in Brazil. Thus, we aim to compile and analyze the official Brazilian data from 1960 until 2017 to enhance the actions of stakeholders, researchers, and decision-makers concerned with Brazilian donkeys and mules.(AU)

Para entender e gerenciar melhor a população de jumentos e mulas é essencial saber informações sobre seu tamanho. Em países em desenvolvimento, esses animais são frequentemente usados como animais de trabalho, principalmente por populações pobres na agricultura e pecuária, mas seu uso está diminuindo no Brasil. Os dados oficiais brasileiros sobre jumentos e mulas começaram a ser mensurados no censo agrícola de 1960 e a partir dessa época foram realizados oito censos agrícolas. Até a redação deste artigo, porém, nenhum trabalho científico analisou os resultados dos censos agropecuários quanto à população de jumentos e mulas no Brasil. Deste modo, nosso objetivo é compilar e analisar os dados oficiais brasileiros de 1960 até 2017, para aprimorar as ações dos stakeholders, pesquisadores e tomadores de decisão preocupados com os jumentos e mulas brasileiras.(AU)

Brazilian donkey slaughter and exports from 2002 to 2019 / Abate e exportação de jumentos no Brasil de 2002 a 2019

The international demand for donkeys has increased sharply in recent years, motivated by China's growing interest in producing ejiao (a traditional medicine made from donkey skin) and, in a smaller proportion, donkey meat. Since the Chinese donkey population dropped by 75.4% in the past 25 years, the country has searched for supply in the international market, mainly in Africa and South America. Aiming to understand the participation of Brazil in this scenario, this paper presents and discusses official data on donkey slaughter and donkey meat and skin exports in Brazil from 2002 to 2019. After the recent news reports of donkey trade-related mistreatment in the Brazilian Northeast, the issue has attracted attention from public authorities, the media, and civil society, but it lacked a quantitative description that could give a tangible dimension to the situation. The interpretation of these data is made with the collaboration of qualitative research methods, emphasizing some aspects of human-animal relations.(AU)

A demanda internacional por jumentos vem aumentando drasticamente nos últimos anos, motivada pelo crescente interesse da China em produzir ejiao (medicamento tradicional feito a partir da pele do animal) e, em menor proporção, carne de jumento. Devido à redução de 75,4% na população de jumentos chineses nos últimos 25 anos, o país tem buscado se abastecer no mercado internacional, principalmente na África e na América do Sul. Com o objetivo de compreender a participação do Brasil neste cenário, este artigo apresenta e discute dados oficiais sobre abate de jumentos e exportação de carne e pele de jumentos no Brasil, de 2002 a 2019. Após a recente divulgação de notícias sobre maus-tratos relacionados ao comércio de jumentos no Nordeste do Brasil, o assunto tem atraído a atenção do poder público, da mídia e da sociedade civil, mas ainda carecia de uma descrição quantitativa que pudesse fornecer uma dimensão tangível à situação. A interpretação desses dados é feita com a colaboração de métodos de pesquisa qualitativa, enfatizando algumas facetas das relações humano-animais.(AU)

Computational classification of animals for a highway detection system / Classificação computacional de animais para sistema de detecção nas rodovias

Vehicle-animal collisions represent a serious problem in roadway infrastructure. To avoid these roadway collisions, different mitigation systems have been applied in various regions of the world. In this article, a system for detecting animals on highways is presented using computer vision and machine learning algorithms. The models were trained to classify two groups of animals: capybaras and donkeys. Two variants of the convolutional neural network called Yolo (You only look once) were used, Yolov4 and Yolov4-tiny (a lighter version of the network). The training was carried out using pre-trained models. Detection tests were performed on 147 images. The accuracy results obtained were 84.87% and 79.87% for Yolov4 and Yolov4-tiny, respectively. The proposed system has the potential to improve road safety by reducing or preventing accidents with animals.(AU)

As colisões entre veículos e animais representam um sério problema na infraestrutura rodoviária. Para evitar tais acidentes, medidas mitigatórias têm sido aplicadas em diferentes regiões do mundo. Neste projeto é apresentado um sistema de detecção de animais em rodovias utilizando visão computacional e algoritmo de aprendizado de máquina. Os modelos foram treinados para classificar dois grupos de animais: capivaras e equídeos. Foram utilizadas duas variantes da rede neural convolucional chamada Yolo (você só vê uma vez) ­ Yolov4 e Yolov4-tiny (versão mais leve da rede) ­ e o treinamento foi realizado a partir de modelos pré-treinados. Testes de detecção foram realizados em 147 imagens e os resultados de precisão obtidos foram de 84,87% e 79,87% para Yolov4 e Yolov4-tiny, respectivamente. O sistema proposto tem o potencial de melhorar a segurança rodoviária reduzindo ou prevenindo acidentes com animais.(AU)

Case report: the use of the AWIN welfare assessment protocol to monitor a group of abandoned donkeys / Estudo de caso: o uso do protocolo AWIN de avaliação de bem-estar para monitorar um grupo de jumentos abandonados

The objective of this study was to reach a diagnosis of the living conditions of abandoned donkeys kept in a restricted farm area through the assessment of their welfare level utilizing the AWIN protocol as a methodological tool. These animals were supposed to be sent to slaughter, but after the activity was temporarily banned, they were abandoned by traders. The protocol of welfare assessment was associated with general environmental and sanitary conditions. Information regarding the mortality rates was also gathered. According to the welfare assessment results, the living conditions of these animals were acceptable in some areas, despite the insufficient shade and shelter, a 3-month food restriction period, and a mortality rate of over 70%. These results demonstrate that welfare assessment protocols must be adapted to crises and databases for welfare indicators in diverse conditions must be created.(AU)

O objetivo deste estudo foi formular um diagnóstico das condições de vida de jumentos abandonados mantidos em uma área restrita de uma propriedade através da avaliação de seu nível de bem-estar, utilizando o protocolo AWIN como ferramenta metodológica. Estes animais seriam destinados ao abate, porém, depois da suspensão temporária da atividade, foram abandonados pelos proprietários. O protocolo de avaliação de bem-estar foi associado às condições ambientais e sanitárias gerais. Informações sobre os índices de mortalidade também foram coletadas. De acordo com os resultados da avaliação de bem-estar, as condições de vida destes animais estavam aceitáveis em algumas áreas, embora não houvesse sombreamento e abrigo suficientes, um período de restrição alimentar de 3 meses e um índice de mortalidade acima de 70%. Estes resultados demonstram que protocolos de avaliação de bem-estar devem ser adaptados a situações de crise, e bancos de dados para indicadores de bem-estar em condições diversas devem ser criados.(AU)

The union between technical knowledge and activism as a tool to save the donkey / A união entre o conhecimento técnico e o ativismo de proteção animal como ferramenta para salvar os jumentos

This case report presents the importance of articulation between legal professionals with the expertise of those who work in different areas of animal science and the activists of animal cause. The report is based on the experience that took place in the interior of the State of Bahia, with a donkey herd, the target of foreign groups interested in donkey hide exploration. The animals were rescued from mistreatment and slaughter, thanks to efficient legal work, aided by several areas of the veterinary sciences, and supported politically by the movement of animal activists. The union between activism and technical knowledge in the areas of health, breeding, nutrition, animal welfare, and legal knowledge is a tool that should not be overlooked. On the contrary, it has proved effective, confirming a strong and innovative link capable of saving animals, promoting their welfare, generating technical knowledge, and new and promising proposals for intersectoral action.(AU)

Este caso relata a experiência da articulação entre profissionais da área jurídica com a expertise daqueles que atuam nas diversas áreas da ciência animal e os militantes do ativismo da causa animal. O relato de caso toma por base a experiência ocorrida no interior do estado da Bahia, no Brasil, com um rebanho de jumentos, alvo de grupos estrangeiros interessados na exploração de pele. Os animais foram salvos de maus-tratos e do abate graças ao eficiente trabalho jurídico, auxiliado por várias áreas da ciência animal e apoiado politicamente pelo movimento de ativistas da causa animal. A união entre o ativismo e o conhecimento técnico é poderosa ferramenta capaz de estabelecer relações, conexões e promover a divulgação do conhecimento na comunidade e, principalmente, salvar a vida de animais humanos e animais não humanos.(AU)

The reality of the donkey's exploitation for the hide trade in Brazil: disease outbreaks and animal welfare compromised in rescued donkeys / A realidade da exploração de jumentos para exportação de pele no Brasil: surtos sanitários e bem-estar animal comprometido em asininos resgatados

About 800 donkeys that were confined in a restrictive area used in a manner comparable to a warehouse for receiving donkeys for slaughter were abandoned. After receiving reports of mistreatment, civilians acted to save the animals. A task force was organized that planned veterinary and zootechnical actions and activities for daily health management, feeding, and clinical care to attend to the abandoned donkeys. Positive cases were diagnosed for glanders, equine infectious anemia, equine herpesvirus, and equine babesiosis. The objective of this communication is to bring to the attention of the scientific community the interventions in the area of animal health and welfare, to address the episode of northeast donkeys that were victims of international trade. It is fundamental to change the approach related to the management of donkeys in Brazil, and appeal to the necessity to identify ethical and sustainable ways to incorporate donkeys in Brazil in the 21st century.(AU)

Cerca de 800 jumentos confinados em uma área utilizada como entreposto de recebimento e encaminhamento de jumentos para abate foram abandonados. Após denúncias de maus tratos, a sociedade civil atuou para salvar os animais. Foi constituída uma força tarefa que planejou ações e atividades veterinárias e zootécnicas atuando diariamente no manejo sanitário, na alimentação e nos cuidados clínicos na assistência aos jumentos abandonados. Foram diagnosticados casos positivos de mormo, anemia infecciosa equina, herpesvírus equino e babesiose equina. O objetivo desta comunicação foi relatar para a comunidade científica as intervenções nas áreas de saúde e bem-estar animal no episódio dos jumentos nordestinos vítimas do comércio internacional. É fundamental mudar a abordagem em relação ao manejo de jumentos no Brasil e apelar para a necessidade de identificar maneiras éticas e sustentáveis de incorporar os jumentos no Brasil do século 21.(AU)

Stereotypic Behavior in Sows Is Related to Emotionality Changes in the Offspring.

Some effects of expressing stereotypic behavior have not yet been elucidated. During gestation, the environment has the potential to interfere with offspring development and to have prenatal or longer-term consequences. We tested the hypothesis that the occurrence of stereotypic behavior during gestation could affect the phenotype of the offspring. Twenty-eight pregnant sows were studied by comparing two groups differing in the amount of stereotypy shown. We analyzed emotionality in the offspring from sows showing high or low stereotypy frequency using the open field and novel object tests. In the open field test, piglets from sows with a high rate of stereotypies walked more in central sectors (p < 0.0001) and lateral sectors (p = 0.04) than piglets from sows with a low rate of stereotypies. In the novel object test, the offspring from low stereotypy sows vocalized more (p = 0.008). We demonstrate for the first time that the stereotypic behavior by the mother during gestation changes the phenotype of the offspring, in particular, their emotionality.

Maternal stress in sheep during late pregnancy influences sperm quality in early puberty of the offspring.

Stress at the end of sheep gestation can damage the reproductive development of young males. The aim of the present study was to evaluate the effects of LPS administration in the last third of sheep pregnancy on the reproductive parameters of prepubertal rams. Thirty-six pregnant nulliparous ewes (12 ±â€¯2 months old; 45 ±â€¯6 kg) were assigned to two treatments, LPS (E. coli; 0.8 µg kg-1) and control (placebo/saline) administered in late pregnancy (120 days post-conception). The animals gave birth to 17 male lambs (11 LPS; 8 control). Reproductive development of the young rams was analyzed from 5 to 12 months of age. A completely randomized design in double factorial scheme was used. The data were analyzed by analysis of variance. The model included treatment (LPS; control), age as main effects and their interactions, and the animal as a repeated measure. Means were compared by the PDIFF-SAS (Pr > |t|) at P < 0.05. An effect of age was observed for scrotal circumference, testicular consistency, homogeneity of testicular parenchyma, vascularization, semen quantity and quality, and blood testosterone concentration (P < 0.05). LPS increased sperm defects (P < 0.05) but an interaction with age was not observed (P > 0.05) with higher abnormalities only during months 8 and 9 (P < 0.05) and not thereafter. In summary, LPS did not cause long-term damage to testicular morphology analyzed from the onset of puberty to sexual maturity. However, LPS treatment affected sperm morphology during early puberty of the offspring.