RESUMEN
The authors systemically reviewed the fast development of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and its clinical application in the past decades. The advantages of this objective technique include real time scanning, easy manipulation and no harm to the subjects examined. Combined with pattern recognition methodology and further confirmation with the clinical and pathological diagnosis, the goal of fast differentiation of malignancy from benign lesions could be achieved. ATR-FTIR spectroscopy technique has shown high differential capacity for benign and malignant tissues such as thyroid, breast and pulmonary diseases. ATR-FTIR spectroscopy has being applied in investigating the differential value (the sensitivity, specificity, and accuracy) of metastatic lymph nodes in thyroid and breast cancer with encouraging results. ATR-FTIR technique would become a promising tool in tissue diagnosis intra-operatively. ATR-FTIR spectroscopy has also been widely applied in detecting bio-fluid to differentiate diseases. The serum ATR-FTIR spectroscopy has the ability of reflecting disease-related information in a fingerprint manner with little amount of blood. Several published articles have covered diseases such as glioma, chest pain, prostate cancer, renal failure, Alzheimer's disease, and ovarian cancer. The results of these researches have proved the efficacious discriminate value of this method. As ATR-FTIR spectroscopy has the potential of fast analysis, accurate diagnosis, and low cost-effective value. It would become one of the most important assisting diagnosis tools in future. Follow-up study should focus on enhancing sample quality and enlarging sample size to have further prospective clinical application.
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Análisis de Fourier , Espectroscopía Infrarroja por Transformada de Fourier , Neoplasias de la Mama , Estudios de Seguimiento , Humanos , Masculino , Neoplasias de la PróstataRESUMEN
To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of lymph node metastasis in gastric cancer by Fourier transform infrared (FTIR) spectrometry. FTIR spectra of surgically removed fresh lymph nodes were measured by FTIR via probe of attenuated total reflection (ATR). For each spectrum, 13 bands were indentified and assigned between 3 000 and 1 000 cm(-1). Peaks in the spectra were measured and relative intensity ratios were calculated and compared between the spectra of Metastatic lymph nodes (MLN) and Non-metastatic lymph nodes (NMLN). Standard statistic analysis was performed. 720 lymph nodes were measured in 38 gastric cancer patients. Results show that there were significant differences between the FTIR of 540 MLN and 180 NMLN. (1) For the band related to nucleic acid: The ratios of I1240/I1460 (p = 0.015) and I1080/I1460 (p = 0.034) increased in MLN, which shows that the relative quantity of nucleic acid was more in MLN than that in NMLN. (2) For the bands related to protein: The ratios of I1640 /I1460 (p = 0.001) and I146/I1460 (p = 0.027) increased in MLN, which shows that the relative quantity of protein was more in MLN. (3) For the bands related to lipid: The ratio of I2855/I460 and I1740/I1460 decreased in MLN FTIR spectrum, indicating the lower relative quantity of lipid in MLN. (4) For the bands related to carbohydrate: The ratio of I1160/I1460 (p = 0.023) decreased in MLN FTIR spectrum, indicating the lower relative quantity of carbohydrate in MLN. The results demonstrate that the FTIR spectroscopy technique maybe develop into a promising method for in situ and quick intraoperative differential diagnosis of lymph node metastasis in gastric cancer.
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Metástasis Linfática/diagnóstico , Neoplasias Gástricas/patología , Carbohidratos , Humanos , Lípidos , Ganglios Linfáticos/patología , Ácidos Nucleicos , Proteínas , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Cancer is a disease that does great harms to the health of human beings. FT-IR spectroscopy could identify variability at the molecular level in biological specimens. It is a rapid and noninvasive method, which could be used intraoperatively to modify surgical procedures. The aim of this paper is to identify and separate cancer from colitis in endoscopic colon biopsies through the use of FT-IR spectroscopy. A total of 88 endoscopic colon samples, including 41 cases of colitis and 47 cases of colon cancer, were obtained. Specimens were placed on an ATR accessory linked to FT-IR spectrometer with a MCT detector for greater stability and sensitivity. Later, specimens were sent for the histological examination as the reference in the spectral analysis. 41 colitis and 47 cancer specimens were compared. Spectra preprocessed with smoothing and normalization were used for discrimination analysis. PCA was processed to simplify the spectrum data set. Naive Bayes classifier model was constructed for diagnostic classification. Leave-one-out cross-validation method was utilized to assess the discrimination results. The sensitivity of FT-IR detection for cancer achieves 97.6%. The results showed that colon cancer could be distinguished from colitis with high accuracy using FT-IR spectroscopy and chemometrics.
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Biopsia/métodos , Colitis/diagnóstico , Neoplasias del Colon/diagnóstico , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Adulto , Anciano , Teorema de Bayes , Neoplasias del Colon/patología , Endoscopía/métodos , Femenino , Humanos , Masculino , Oncología Médica/métodos , Persona de Mediana Edad , Análisis de Componente Principal , Reproducibilidad de los ResultadosRESUMEN
To explore the feasibility of Fourier transform infrared spectrometry (FTIR) for the diagnosis of colon neoplasms, fresh samples of eighteen cancers and ten adenomas were collected during colon surgery and were measured by FTIR via probe of attenuated total reflection (ATR). The peak position and the intensity of all bands were measured and compared between the malignant and benign groups. Results show that the FTIR of malignant neoplasm was different from that of benign one. (1) For the bands related to lipid: The ratio of I2 925 /I1 460 (p = 0.018) and I1 740/I1 460 (p = 0.009) decreased in cancer FTIR spectrum, indicating the lower relative quantity of lipid in malignant neoplasm. (2) For the bands related to protein: The ratios of I3 375/ I1 460 (p = 0.012) and I1 550/I1 460 (p = 0.041) increased in cancer, which shows that the relative quantity of protein was more in cancer than that in adenoma. (3) For the band related to nucleic acid: In malignant tissue the peak position in 1 080 cm(-1) shifted toward a higher wave number (p = 0.039), compared with that in benign one. And the ratio of I1 083/I1 460 increased significantly (p = 0.036), illustrating the higher relative quantity of nucleic acid to lipids in malignancy. (4) The peak position of 1 305 cm(-1) shifted to a lower wavenumber (p = 0.041), which should be determined in further research It is concluded that our initial research showed that the FTIR maybe develops into a promising method for rapid differential diagnosis of colon malignant and benign neoplasms.
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Neoplasias Colorrectales/diagnóstico , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Adenoma/diagnóstico , Colon/fisiopatología , HumanosRESUMEN
In the present investigation, 24 cases of meningoima tissues (including 12 cases of benign tumor and 12 cases of malignant tumor 12) were detected using FT-mid-IR spectroscopy linked with attenuated total reflectance (ATR) accessory. These FTIR spectra obtained from the above-mentioned meningoima tissues were analyzed and compared. Significant differences were found in the spectral features of different kinds of meningoima tissues for example fibrous type meningoima and endothelioid meningoima; and for the same type of meningoima tissues the significant differences in the spectram features can be also observed with the increase of grade malignancy. The malignant tumor can be distinguished primarily from benign tumor by the changes of position, shape and intensity of infrared absorption bands, particularly in the ranges of 1 000-1 500 cm(-1). The results show that the peak position of the bands (such as 1 160 cm(-1)) can be used to distinguish the characteristic of meningoima which are in agreement with the pathological results. The accuracy is larger than 85%. These results demonstrate that FT-mid-IR spectroscopy exhibits prospect to develop a novel, non-invasive and rapid method for the diagnosis the brain tumors.
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Neoplasias Meníngeas/diagnóstico , Meningioma/diagnóstico , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Humanos , Neoplasias Meníngeas/patología , Meningioma/patologíaRESUMEN
The blood glucose concentrations of volunteers from diabetes patients and healthy adults (all patients and volunteers who joined this experiment gave their consents) were measured by using a modified WQF-200 FTIR spectrometer with a newly designed ATR accessory from the Beijing Rayleigh Analytical Instrument Corp. The determination basis for this technique from the physiological point of view is also discussed based on the experimental results, which indicated that the glucose measured by the FT-Mid-IR ATR instrument is from the secretions on the skin and glucose components within the body. The secreted glucose components will increase with the time increasing. The authors' previous study demonstrated 1 120 cm(-1) band as an index to characterize the blood glucose. During the experiments, the authors used the band of 1 455 cm(-1) as internal standard because of its stability, and because the relative intensity of I1 120/I1 455 band possesses the higher sensitivity. Meanwhile, from the spectra, the relative intensity of I1 120/I1 455 band of the glucose in both sources exhibits a linear relationship with blood glucose concentration within the body. The dried blood has the similar spectra as fingers'. The fingers' spectra will exhibit higher sensitivity if the time of measurement is longer after washing hand, and the results showed that when measured 10 minutes after washing hand, the sensitivity will be higher than that when measured 4 minutes after washing hand. All the results can be used in promoting a convenient, rapid and noninvasive way to monitor the continuous variation of blood glucose concentration of diabetes patients in real time.
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Glucemia/análisis , Monitoreo Fisiológico/métodos , Espectroscopía Infrarroja por Transformada de Fourier , Adulto , Diabetes Mellitus , HumanosRESUMEN
Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflection(ATR) probe was used to study the benign and malignant tissues of gallbladder. FTIR spectra of 18 tissues of gallbladder carcinoma and 139 benign tissues were collected and analyzed. The results demonstrated that bands related to lipid, carbohydrate, and nucleic acid from malignant samples were significantly different from those of benign ones: (1) The peak positions of 1 167 and 1 123 cm(-1) shifted toward lower wave number (P < 0.05) but that of 1 309 cm(-1)' to the higher one (P < 0.05); (2) The relative intensity ratios of I2 856/I1 461, I1 167/ I1 461, I1 123/I1 461, I1 082/I1 461, increased significantly (P < 0.05) (3) The half-widths of 1 167 and 1 082 cm(-1) increased significantly (P < 0.05), whereas the half-width of 1 461 cm(-1) decreased significantly (P < 0.05). (4) The probability of 1 750 cm(-1) band of gallbladder carcinoma tissues increased significantly (P < 0.05).
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Carcinoma/patología , Vesícula Biliar/patología , Espectroscopía Infrarroja por Transformada de Fourier , Carbohidratos , Humanos , Lípidos , Ácidos NucleicosRESUMEN
The aim of the present research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared spectra) of the cultured carcinoma cell and nucleus with FTIR spectroscopy, and investigating the special spectral features of the carcinoma cell and nucleus compared with the carcinoma tissues. In this paper, the gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna 5700-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gallbladder carcinoma cell (GBC-SD) and nucleus were centrifuged to provide a small pellet of cell and nucleus for FTIR analysis. The cell and nucleus pellet was then placed on the OMNIC sampler. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cell and nucleus (GBC-SD) and that of the corresponding gallbladder tissues. Several infrared spectral features of the carcinoma cell and nucleus were obtained. All the results suggest that the spectral features of the carcinoma cell and nucleus can be well reflected by that of the carcinoma tissue, though the later is more complicated, which might originate from the intrinsic complexity of the tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
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Núcleo Celular/química , Núcleo Celular/patología , Neoplasias de la Vesícula Biliar/química , Neoplasias de la Vesícula Biliar/patología , Línea Celular Tumoral , Centrifugación , Neoplasias de la Vesícula Biliar/diagnóstico , Humanos , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
BACKGROUND: Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line. METHODS: The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue. RESULTS: Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue. CONCLUSION: The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
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Neoplasias de la Vesícula Biliar/diagnóstico , Vesícula Biliar/patología , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Línea Celular Tumoral , Diagnóstico Precoz , Neoplasias de la Vesícula Biliar/patología , Humanos , Técnicas In VitroRESUMEN
OBJECTIVE: To explore a novel non-invasive method in detection of thyroid cancer by Fourier transform infrared (FTIR) spectroscopy. METHODS: Surface FTIR spectra of 15 cases of thyroid cancer and 51 cases of normal subjects were collected. 22 variables of 11 bands including peak positions and relative intensities were measured and all data were statistically analyzed. RESULTS: In the cancer group: (1) the peak position of 1743 cm(-1) was shifted toward higher wave number (P < 0.05), and that of 1250 cm(-1) to the lower (P < 0.05), when compared to those of normal ones. (2) The relative intensity ratios of I(1546)/I(1460), I(1250)/I(1460), I(1120)/I(1460), I(1080)/I(1460) were significantly increased (P < 0.05). (3) The presence rate of band of 1340 cm(-1) was significantly decreased (P < 0.05). CONCLUSION: FTIR surface spectra may become a novel powerful non-invasive approach of detecting thyroid cancer in regular routine check-up.
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Espectroscopía Infrarroja por Transformada de Fourier/métodos , Glándula Tiroides/química , Neoplasias de la Tiroides/química , Neoplasias de la Tiroides/diagnóstico , HumanosRESUMEN
To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared (FTIR) spectroscopy, 26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery. And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection (ATR) without pretreatment immediately after resection. For each spectrum, 12 bands were identified and assigned between 3 800 and 1 000 cm(-1). The peak position was found, and the intensity and area of the bands were measured; The ratios of the intensity and area were calculated. Standard statistic analysis was performed. The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue: 1. The bands related to lipid: The peak of 2 925 cm(-1) shifted to lower wave number significantly (P = 0.033); 2. The bands related to nucleic acid and lipids: the ratios of I1 083/ I1 460 (P = 0.005), S1 083 /S1 460 (P = 0.001) and S1 240/S1 460 (P = 0.025) raised significantly, indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy. 3. The bands related to protein: The ratios of I1 550/I1 083 (P = 0.000) and S1 550/S1 083 (P = 0.000) reduced significantly, implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably. Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.
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Colangiocarcinoma/patología , Espectroscopía Infrarroja por Transformada de Fourier , Colangiocarcinoma/diagnóstico , Humanos , Lípidos/análisis , Ácidos Nucleicos/análisis , Estructura Secundaria de Proteína , Proteínas/análisisRESUMEN
The aim of the study is to explore the possibility of detecting metastatic lymph nodes by FTIR spectra during the surgery of thyroid cancer. The FTIR spectra of 20 metastatic lymph nodes and 69 non-metastatic ones were collected via an ATR (attenuated total reflectance) probe. For each spectrum, 28 variables of 13 bands including peak positions and relative intensities were measured. The variables of metastatic lymph node were compared to those of non-metastatic ones using standard statistic methods. The results indicated that the FTIR spectra of metastatic lymph nodes were significantly different from non-metastatic ones in the bands related to protein, lipid, nucleic acid and carbohydrate. (1) Variations of bands related to protein: The relative intensity ratios of I3 280 /I1 460, I1 640 /I1 460 and I1 546/I1 460 increased significantly (P < 0.05); (2) Variations of bands related to lipid: The relative intensity ratios of I1 743 /I1 460 decreased significantly (P < 0.05). On the contrary, I 400 /I1 460 increased significantly (P < 0.05); (3) The peak positions of 1 165 and 1 120 cm(-1) which were all assigned to carbohydrate shifted toward higher wave number (P < 0.05). The relative intensity ratio of I1 165 /I1 460 decreased significantly (P < 0.05); (4) The peak positions of 1 085 cm(-1) related to nucleic acid shifted to the lower wave number (P < 0.05); (5) Other undetermined bands: The relative intensity ratios of I1 303 /I1 460 and I1 303 /I1 240 increased significantly (P < 0.05). FTIR spectroscopy could be a reliable and practicable method for metastatic lymph nodes diagnosis in the operation of thyroid cancer. It could be applied in detecting metastatic lymph nodes which can not be determined by palpation in surgery.
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Ganglios Linfáticos/patología , Espectroscopía Infrarroja por Transformada de Fourier , Neoplasias de la Tiroides/patología , Humanos , Metástasis LinfáticaRESUMEN
The aim of this research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared) spectra of the cultured carcinoma cells with FTIR spectroscopy and investigating the special spectral features of the carcinoma cells compared with the carcinoma tissues. In the present paper, the gastric carcinoma tissues confirmed by histology were measured using a Nicolet Magna750-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gastric carcinoma cells (SGC7901) were centrifuged to provide a small pellet of cells for FTIR analysis. The cell pellet was then placed on a specially designed salt plate made of BaF2. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cells (SGC7901) and that of the corresponding gastric tissues. Several infrared spectral features of the carcinoma cells were obtained: the different bands between cells and tissues locate in the range of 3 000-3 600 cm(-1) and 1 640 cm(-1) which are the range of the hydroxy stretching and blending bands of H2O. There are more H2O out of carcinoma cells in carcinoma tissues, so the strong bands of H2O cover the distinctive bands of carcinoma cells in carcinoma tissues. Although the carcinoma tissue is more complicated, which might originate from the intrinsic complexity of the tissue, the results suggest that the spectral features of the carcinoma cells can be well reflected by that of the carcinoma tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
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Carcinoma/patología , Absorción , Animales , Línea Celular Tumoral , Humanos , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
In the present paper, 17 cases of freshly resected malignant thyroid tissue samples and 23 cases of benign thyroid tissue samples were characterized using FTIR spectroscopy with an ATR probe. Statistic analysis indicated that the FTIR spectra of cancerous tissue were significantly different from those of benign tissue. In comparison with normal tissues, the FTIR spectra of malignant thyroid tissues possess the following features: (1) Variation of bands related to protein: Amide I band shifted to lower wave number significantly (P<0.01), while amide II band shifted to higher wave number(P<0.05), and the ratios of I1640/I1460 and I1640/I1550 were raised (P<0.01). The authors can conclude that the amounts and the secondary structure of protein in malignant tissue changed. (2) The peaks at 2955, 2920, 2870, 2850 and 1740 cm(-1) appeared less frequently suggesting that the relative quantity of lipid in malignant tissue decreased significantly; (3) Variation of bands related to nucleic acid: The band of 1241 cm(-1) shifted to (1238.29+/-2.87)cm(-1), and the ratios of I1080/I1460 were raised significantly(P<0.05). The most possible reason for the changes was that the mutation of DNA and the amounts of nucleic acid had increased in malignant neoplasms. Experimental results show that the malignant thyroid tissues can be distinguished from the benign tissues by their infrared spectra. This approach proves that FTIR spectroscopy is a reliable and practicable method for thyroid cancer diagnosis in operations. The authors have expanded their research on detecting the thyroid cancer FTIR spectra via percutaneous and in vivo, and have achieved a positive result which is going to be reported in another paper.
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Espectroscopía Infrarroja por Transformada de Fourier/métodos , Glándula Tiroides/patología , Neoplasias de la Tiroides/química , Neoplasias de la Tiroides/patología , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Glándula Tiroides/química , Glándula Tiroides/cirugía , Neoplasias de la Tiroides/diagnóstico , Neoplasias de la Tiroides/cirugía , Adulto JovenRESUMEN
In the present work, 20 patients with salivary pleomorphic adenoma were recruited for FTIR spectroscopic measurement. These obtained FTIR spectra were analyzed and compared. It was found that there were significant differences in the spectral features of the skin covering normal salivary gland, pleomorphic adenoma, and carcinoma change of pleomorphic adenoma, such as the changes in peak position, band shape and relative intensity of the bands in the ranges of 1000-1800 cm(-1) and 2800-3000 cm(-1). Pathological diagnosis demonstrated that 2 of the 20 patients suffered actually carcinoma change of pleomorphic adenoma, which is in good agreement with the result of FTIR spectroscopicmeasurement. FTIR spectroscopic m ethodsuggested that pleomorphic adenoma is the intermediate between normal salivary gland and carcinoma change of pleomorphic adenoma.
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Adenoma Pleomórfico/química , Neoplasias de las Glándulas Salivales/química , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Adenoma Pleomórfico/diagnóstico , Adenoma Pleomórfico/patología , Humanos , Neoplasias de las Glándulas Salivales/diagnóstico , Neoplasias de las Glándulas Salivales/patologíaRESUMEN
Naphthenic acid (NA) was mixed with PC88A in heptane, and 10 moL x L(-1) KOH aqueous solution was used to saponify the solution subsequently. The transparent and clear appearance of the obtained solution indicated the formation of w/o micell and microemusion. In the present study, micell with high saponification percentage (80%) was used to extract neodymium. The organic phase structure was characterized using FTIR spectroscopy in the extraction process. IR spectra indicate that the characteristic peaks of nu(COOH), nu(s)(COO-) and nu(P=O) shift to lower wavenumbers as the loading of Nd in organic phase increases. The results demonstrated that Nd(III) are coordinated to P=O and COO- groups simultaneously, and the supramolecules with self-assembly structure was formed containing PC88A and NA, which were binding with Nd(III) in the extracted organic phase.
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AIM: To determine if Fourier-transform infrared (FT-IR) spectroscopy of endoscopic biopsies could accurately diagnose gastritis and malignancy. METHODS: A total of 123 gastroscopic samples, including 11 cases of cancerous tissues, 63 cases of chronic atrophic gastritis tissues, 47 cases of chronic superficial gastritis tissues and 2 cases of normal tissues, were obtained from the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance (ATR) accessory was linked to a WQD-500 FT-IR spectrometer for spectral measurement followed by submission of the samples for pathologic analysis. The spectral characteristics for different types of gastroscopic tissues were summarized and correlated with the corresponding pathologic results. RESULTS: Distinct differences were observed in the FT-IR spectra of normal, atrophic gastritis, superficial gastritis and malignant gastric tissues. The sensitivity of FT-IR for detection of gastric cancer, chronic atrophic gastritis and superficial gastritis was 90.9%, 82.5%, 91.5%, and specificity was 97.3%, 91.7%, 89.5% respectively. CONCLUSION: FT-IR spectroscopy can distinguish gastric inflammation from malignancy.
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Gastritis/diagnóstico , Gastroscopía , Espectroscopía Infrarroja por Transformada de Fourier , Neoplasias Gástricas/diagnóstico , Adulto , Anciano , Anciano de 80 o más Años , Biopsia , Femenino , Gastritis/patología , Humanos , Masculino , Persona de Mediana Edad , Espectroscopía Infrarroja por Transformada de Fourier/normas , Neoplasias Gástricas/patología , Factores de TiempoRESUMEN
AIM: Real-time and rapid identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology. METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies. RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research. CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopic method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.
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Neoplasias Colorrectales/diagnóstico , Tecnología de Fibra Óptica , Espectroscopía Infrarroja por Transformada de Fourier , Anciano , Neoplasias del Ciego/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Fibras ÓpticasRESUMEN
BACKGROUND: Fourier transform infrared (FT-IR) spectroscopy is an effective tool for investigation of chemical changes at the molecular level. We previously demonstrated that FT-IR spectroscopy can reliably distinguish multiple types of carcinoma from healthy tissue. Because various stomach diseases are common, it is important to explore a noninvasive and rapid method to detect malignancy and gastritis in endoscopic biopsies. Our aim was to classify endoscopic biopsies into healthy, gastritis, and malignancy through the use of FT-IR spectroscopy. METHODS: A total of 103 endoscopic samples, including 19 cases of cancer, 35 cases of chronic atrophic gastritis, 29 cases of chronic superficial gastritis, and 20 healthy tissue samples, were obtained at the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance accessory was linked to a WQD-500 FT-IR spectrometer for biopsy measurement. The spectral characteristics for different types of tissues were correlated with the corresponding pathology results. The gastric biopsies were classified by FT-IR spectroscopy and a discriminant analysis method. RESULTS: There were significant differences in the FT-IR spectra of four types of gastric biopsies. The discriminant analysis results demonstrated that the sensitivity of FT-IR detection for healthy, superficial gastritis, atrophic gastritis, and gastric cancer was 90%, 90%, 66%, 74%, respectively, which could help satisfy clinical diagnostic requirements. CONCLUSION: FT-IR spectroscopy can distinguish disease processes in gastric endoscopic biopsies.
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Gastritis/diagnóstico , Neoplasias Gástricas/diagnóstico , Biopsia , Enfermedad Crónica , Gastritis/patología , Gastroscopía , Humanos , Análisis Multivariante , Espectroscopía Infrarroja por Transformada de Fourier , Estómago/patología , Neoplasias Gástricas/patologíaRESUMEN
In this paper, the authors have reviewed their investigation on the clinical detection of tumor tissues by infrared spectroscopy in recent ten years. Based on the comparison of different IR spectroscopic methods such as IR transmission spectroscopy, micro-IR spectroscopy etc, the authors found the good consistency of the results of ATR (attenuated total reglection) IR spectroscopic method with those of pathological biopsy. The authors have directly measured the IR spectra of frozen tissues stored in liquid nitrogen and freshly resected tissues, and have realized the measurement of tumor tissues in vito during the operation process using a specially designed IR spectrometer connected with a mid-IR fiberoptic with an ATR probe. The authors have investigated the malignant and normal tissues including parotid, esophagus, stomach, colon, liver, gallbladder, breast, thyroid etc. and compared with the pathological results. The accuracy of this novel IR detection method is more than 90%.