RESUMEN
The integrated toxicities of 4-tert-octylphenol (t-OP) on Siganus oramin were investigated by dietary administration at doses of 5, 25 and 125 mg/kg body weight over 28 days. Significant increase was observed in the activity of hepatic glutathione S-transferase at 125 mg/kg on both day 14 and 28 in males, and at all doses on day 28 in females, and in hepatosomatic index at 25 mg/kg on day 14 in both sexes. Plasma levels of testosterone and cortisol decreased significantly at all doses on day 28. Histopathologic changes in liver, spleen, intestine and testis deteriorated with increasing doses and duration. The results suggest that S. oramin is sensitive to t-OP, and the above endpoints may be potential biomarkers for evaluating toxicities of environmental pollutants such as t-OP.
Asunto(s)
Fenoles/toxicidad , Tensoactivos/toxicidad , Contaminantes Químicos del Agua/toxicidad , Alimentación Animal , Animales , Biomarcadores/sangre , Relación Dosis-Respuesta a Droga , Monitoreo del Ambiente/métodos , Femenino , Glutatión Transferasa/metabolismo , Hidrocortisona/sangre , Intestinos/efectos de los fármacos , Intestinos/patología , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/patología , Masculino , Perciformes , Bazo/efectos de los fármacos , Bazo/patología , Testículo/efectos de los fármacos , Testículo/patología , Testosterona/sangre , Pruebas de ToxicidadRESUMEN
P53 gene protein expression in breast benign disease and cancer was studied quantitatively using flow cytometry and cellular immunofluorescence staining technique. The results showed that P53 positive expression rate and content were lower in the benign disease of breast than that of the breast cancer. The content of P53 protein expression was also associated with the DNA ploidy in breast cancer. The content of P53 protein expression was higher in tumors with aneuploid cells than those with the diploid ones. The five year survival rate were also lower in P53 protein expression positive cases than the P53 negative one's.