Modification of the Francis Medium for the Conversion of Histoplasma capsulatum to the Yeast-Like Growth Phase.

We propose a modification of Francis agar used for identification of the causative agent of histoplasmosis by in vitro conversion of the mycelial culture to the yeast-like growth phase. For improving of the growth characteristics of the medium, we used FT-agar with glucose-vitamin additives developed for culturing of the tularemia causative agent. The modified Francis medium is characterized by significantly higher growth properties and allowed 10-fold increasing the number of CFU of yeast-like cells of both American and African histoplasmosis causative agent.

[Transport medium for pathogenic pseudomonads]. / Transportnaia sreda dlia patogennykh psevdomonad.

A transporting medium was designed for the transportation and preservation for 5 to 7 days of material infected with agents of malleus and melioidosis. Foreign contaminating microorganisms gradually die in this medium, whereas solitary cells of malleus and melioidosis agents (100 bacterial cells per 0.1 ml) survive.