RESUMEN
OBJECTIVE: To study ultrasonic naked gene delivery and its effect control, and to investigate the new methods, which are easy-to-use, reliable and non-cytotoxic for application in gene therapy so as to build an optimal parameter set. METHOD: Suspensions of different kinds of cells were exposed to calibrated ultrasound field (35.1 kHz) with different parameters for gene delivery. Morphology, membrane permeability, naked gene expression efficiency, threshold of cell damage and cell viability were examined by laser scanning confocal microscopy, fluorescent microscopy, flow cytometry and spectrophotometer. RESULT: The plasmid of green fluorescent protein (GFP) was delivered into S180 cells under optimal parameters without cell damage. The transfection rate was about 35.83% +/- 2.53% (n=6) in viable cells. The GFP expression intensity in ultrasound exposure group had a higher fluorescent peak than AVV-GFP group and control group (P<0.001). CONCLUSION: Ultrasound can effectively deliver naked gene into cells using optimal parameters without cell damage. The gene uptake depends on energy accumulation at 90% of cell viability, and the tendency of apoptosis is found in S180 tumor cells.
Asunto(s)
Terapia Genética/métodos , Plásmidos/genética , Transfección/métodos , Ultrasonido , Animales , Membrana Celular/química , Membrana Celular/metabolismo , Supervivencia Celular , Células Cultivadas , Pollos , Eritrocitos , Estudios de Evaluación como Asunto , Genes Reporteros/genética , Proteínas Fluorescentes Verdes/genética , Permeabilidad , Conejos , Sarcoma 180RESUMEN
In this paper, point to point video communication system based on internet is deeply studied. Meanwhile the application system designed practically is introduced. It is emphasized that video compression coding based on low bit rate stream with ITU-T H. 263 agreement and related international standards is realized.
Asunto(s)
Comunicación , Diagnóstico por Computador/métodos , Internet , Telemedicina , Diagnóstico por Computador/instrumentación , Procesamiento de Imagen Asistido por Computador/instrumentación , Procesamiento de Imagen Asistido por Computador/métodos , Almacenamiento y Recuperación de la Información/métodos , Programas Informáticos , Interfaz Usuario-Computador , Grabación de Cinta de Video/métodosRESUMEN
Ultrasound therapy is a new type of therapy technology, there are hyperthermia and high intensity focused ultrasound surgery (HIFUS). Compared with a single frequency system, mutiple frequency system has an additional function to combine power patterns of different frequencies. This function increases the availability of power patterns to treat tumors of various shapes and depths. Therefore, we have in this article proposed a system with the ability to drive ultrasonic phased arrays of multiple resonant frequencies for ultrasound hyperthermia and HIFUS. The results show that this system is able to (1) drive multi-element applicators or phased arrays of a single resonant frequency through the multichannel power amplifiers, (2) concurrently drive transducer with different resonant frequencies, (3) adjust the relative phase and output power of each channel for the scanning ultrasonic focus, and (4) operate each channel with good output stability.