[Clinical Characteristics and Treatment of Blastic Plasmacytoid Dendritic Cell Neoplasm].

OBJECTIVE: To explore the clinical manifestations, diagnosis, treatment and prognosis of blastic plasmacytoid dendritic cell neoplasm(BPDCN). METHODS: The clinical features, bone marrow morphology and immunophenotyping, treatment and prognosis of 4 patients with BPDCN were analyzed retrospectively. RESULTS: 4 patients had bone marrow， spleen and lymph nodes involvement， 2 patients had skin lesions， and 3 patients had central nervous system infiltration． Tailing phenomenon of abnormally cells could be seen in bone marrow. The immunophenotyping showed that CD56, CD4 and CD123 expression was observed in 4 patients， and CD304 in 3 patients． One patient refused chemotherapy and died early. Both patients achieved complete remission after the initial treatment with DA+VP regimen, 1 of them achieved complete remission after recurrence by using the same regimen again． One patient failed to respond to reduced dose of DA+VP chemotherapy, and then achieved complete remission with venetoclax+azacitidine. CONCLUSION: The malignant cells in BPDCN patients often infiltrate bone marrow, spleen and lymph nodes, and have specical phenotypes, with poor prognosis. The treatment should take into account both myeloid and lymphatic systems. The treatment containing new drugs such as BCL-2 inhibitors combined with demethylation drugs is worth trying.

Improving cadmium accumulation by Solanum nigrum L. via regulating rhizobacterial community and metabolic function with phosphate-solubilizing bacteria colonization.

Soil cadmium (Cd) mobilized with phosphate-solubilizing bacteria (PSB), especially for strains effectively colonized in rhizosphere, is an important pathway for promoting its accumulation by Cd-hyperaccumulators. In this study, screened PSB strains, Acinetobacter pittii (AP) and Escherichia coli (EC), were used to evaluate their effects on Cd mobilization in rhizosphere, Cd accumulation by Solanum nigrum L., and rhizobacterial community and metabolic function under different colonization condition. Results indicated that AP or EC inoculated in soils significantly promoted plant growth, and simultaneously motivated Cd accumulation in S. nigrum L. by 119% and 88%, respectively, when compared with that of uninoculated treatment. Higher efficiency colonization of AP contributed to more organic acids (malic, l-proline, l-alanine, and γ-aminobutanoic) production in the rhizosphere soil and Cd accumulation by S. nigrum L., when compared with that of EC treatment. Taxonomic distribution and co-occurrence network analyses demonstrated that inoculation of AP or EC enriched dominant microbial taxa with plant growth promotion function and keystone taxa related to Cd mobilization in the rhizosphere soil, respectively. Inoculated strains up-regulated the expression of genes related to bacterial mobility, amino acid metabolism, and carbon metabolism among rhizobacterial community. Overall, this study provided a feasible method for soil Cd phytoremediation by promoting Cd mobilization with the enhancement of keystone taxa and organic acid secretion based on the high-efficiency colonization of PSB.

Effect of Phosphate-Solubilizing Bacteria on the Mobility of Insoluble Cadmium and Metabolic Analysis.

Phosphate-solubilizing bacteria (PSB) can promote plant growth by dissolving insoluble phosphate. Therefore, PSB may have the potential to improve the mobility of heavy metals in soils and enhance phytoextraction. This study isolated a few PSB strains that could dissolve CdCO3 and solid Cd in soil. Two typical PSB, namely, high- and low-Cd-mobilizing PSB (Pseudomonas fluorescens gim-3 and Bacillus cereus qh-35, respectively), were selected to analyze the metabolic profiles, metabolic pathways, and mechanisms of mobilization of insoluble Cd. A total of 34 metabolites secreted by the two PSB strains were identified. Gluconic acid was the main contributor to Cd dissolution (42.4%) in high-Cd-mobilizing PSB. By contrast, gluconic acid was not secreted in low-Cd-mobilizing PSB. Metabolic pathway analysis showed that gluconic acid was produced by the peripheral direct oxidation pathway. Hence, PSB with peripheral direct oxidation pathway were likely to have high-Cd-mobilizing capacity.