RESUMEN
The present study has the aim to describe morphologically, morphometrically and stereologically some changes in the tongue epithelium of rat fetuses under the effect of cyclosphosphamide. This drug was used in a single 6mg/kg according to the body weight in the tenth pregnancy day while the control group received equal dosage of saline solution by the same duct and in the same day of pregnancy. The fetuses were plunged into fixed solution of Bouin for five days and the heads of then were divided and blocked in paraffin. Histological sections of six-micrometer thickness were performed and then colored with hematoxilin and eosin. It was used morphometrical and stereological analysis after the morphological study. The data were submitted under the statistical non-parametric test of Mann-Whitney. The results showed that the cyclosposphamide usage effect during the rat pregnancy caused a delayed in the embryo fetal growing, a reduction in the placental weight as wells as in the umbilicus cord length. The covering epithelia in the tongue of the treated group showed some changes since there was an epithelial hyperplasia associated with a cell hypotrophy. The fetuses in this group were more premature than the ones in the control.
El estudio tiene como objetivo describir morfológica, morfométrica y estereológicamente algunos cambios en el epitelio de la lengua de fetos de rata bajo los efectos de la ciclofosfamida. Esta droga fue usada en una muestra única de 6mg/kg de acuerdo al peso del cuerpo, en el décimo día de preñez, mientras el grupo control recibió igual dosis de una solución salina, por la misma vía, en los mismos días de preñez. Los fetos fueron sumergidos en solución fijadora de Bouin, por cinco días y las cabezas de ellos fueron cortadas y colocadas en bloques de parafina. Secciones histológicas de 6 µm fueron teñidas con hematoxilina-eosina. Después del estudio morfológico se realizaron análisis morfométricos y estereológicos. Se utilizó el test estadístico no paramétrico de Mann-Whitney. Los resultados mostraron que el uso de la ciclofosfamida provoca efectos durante la preñez de la rata, causando un retardo en el crecimiento de los embriones, una reducción en el peso de las placentas como también, en la longitud del funículo umbilical. El epitelio de revestimiento de la lengua del grupo tratado, mostró algunos cambios desde una hiperplasia epitelial asociada con una hipotrofia celular. Los fetos del grupo tratado fueron más prematuros que los del grupo control.
Asunto(s)
Animales , Femenino , Embarazo , Ratas , Lengua/efectos de los fármacos , Ciclofosfamida/farmacología , Ratas Wistar , Teratología , Antineoplásicos Alquilantes/farmacología , Epitelio/efectos de los fármacos , Feto , CariometríaRESUMEN
Male rats with 250 grs of weight were injected daily with 0.1 ml of B6 vitamin by peritoneal (way paths). The animal were sacrificed at three, seven, 10, 15 and 21 day after the upper incisor extraction. Its hemimaxila were retired and fixed in 10% formalin and after had been embedded in paraffin sections were cut with 6 micrometers pick. The analysis of the sections stained by hematoxylin and eosin when compared with the controls showed that: 1) the blood clot and fibrin net are substituted more rapidly by the granulation tissue; 2) granulation tissue is more plentiful, early and mature; 3) the bone tissue is more plentiful and mature.