Pathological features of bovine pericardium implanted into mice abdominal wall after preservation with glutaraldehyde or glycerin / Aspectos patológicos do implante de pericárdio bovino tratado pelo glutaraldeído e pela glicerina em parede abdominal de camundongos

Background: Lately, the use of biological materials has been widely indicated in surgical procedures to restore structure and function of injured tissues. Bioimplants require different conservation techniques; among these, glutaraldehyde preservation stands out owing to its higher antimicrobial efficiency as compared to glycerin. In view of the need to determine a concentration of glutaraldehyde that can act as a biocide but do not cause undesirable tissue reactions, this study aimed to identify and quantify gross and microscopic tissue alterations after implantation of bovine pericardium, which was preserved in various concentrations of glutaraldehyde, in the abdominal wall of mice. Materials, Methods & Results: Fresh pericardia from 18 bovines were fractioned into 1cm2 samples and treated with a 98% glycerin solution for 30 days (control group), or 0.625%, 1%, and 1.5% glutaraldehyde solution for 18 days (experimental groups). An abdominal muscle fragment was excised from each mouse, and a 1-cm2 fragment of preserved pericardium was implanted in the area. Sixty mice (n = 15 per treatment) divided into groups were observed for 7, 14, and 30 days, and five animals from each group were euthanized at each time point for gross and microscopic examination. Fragments of the implants and adjacent skin lesions were harvested, fixed in formalin, and processed for routine histology [...](AU)

Pathological features of bovine pericardium implanted into mice abdominal wall after preservation with glutaraldehyde or glycerin / Aspectos patológicos do implante de pericárdio bovino tratado pelo glutaraldeído e pela glicerina em parede abdominal de camundongos

Background: Lately, the use of biological materials has been widely indicated in surgical procedures to restore structure and function of injured tissues. Bioimplants require different conservation techniques; among these, glutaraldehyde preservation stands out owing to its higher antimicrobial efficiency as compared to glycerin. In view of the need to determine a concentration of glutaraldehyde that can act as a biocide but do not cause undesirable tissue reactions, this study aimed to identify and quantify gross and microscopic tissue alterations after implantation of bovine pericardium, which was preserved in various concentrations of glutaraldehyde, in the abdominal wall of mice. Materials, Methods & Results: Fresh pericardia from 18 bovines were fractioned into 1cm2 samples and treated with a 98% glycerin solution for 30 days (control group), or 0.625%, 1%, and 1.5% glutaraldehyde solution for 18 days (experimental groups). An abdominal muscle fragment was excised from each mouse, and a 1-cm2 fragment of preserved pericardium was implanted in the area. Sixty mice (n = 15 per treatment) divided into groups were observed for 7, 14, and 30 days, and five animals from each group were euthanized at each time point for gross and microscopic examination. Fragments of the implants and adjacent skin lesions were harvested, fixed in formalin, and processed for routine histology [...]

Bioimplantes de donantes cadavéricos para uso cardiovascular: el valor de una oportunidad / Bioimplants of cadaveric donors for cardiovascular use: value of an opportunity

La alteración anatómica del corazón, trae como consecuencia anormalidades en su funcionalidad; dependiendode la severidad de la lesión llega a ser necesario el reemplazo de algunas estructuras con un implante que puede ser de tipo biológico. Las posibilidades establecidas para el procesamiento de tejidos biológicos de uso cardiovascular, permiten disponer en la actualidad de una variedad de bioimplantes para el manejo quirúrgico de pacientes con diversas patologías. En este sentido se han desarrollado técnicas para el procesamiento, conservación y almacenamiento de tejidos, llamados homoinjertos, los cuales se obtienen de donantes cadavéricos, en los que aún se conserva viabilidad celular. Desafortunadamente, de otro lado, queda el sinsabor de ver frustrada la vida de miles de personas que por violencia u otras causas accidentales pierden su vida; aunque ello incremente las oportunidades de alivio para gran cantidad de pacientes que esperan por un implante biológico producto de un donante; es la ambivalencia que existe entre las oportunidades de salvar vidas y el costo social que representan las víctimas de la violencia que se ofrecen como donantes.

The structural alteration of the heart, entails a functional damage; depending on how severe is the lesion can benecessary the replacement of the structure with an implant that can be of a biological type. The opened possibilities to biological tissue processing for cardiovascular use, allow to have at the present time a variety of bioimplants for the surgical treatment of patients with diverse pathologies. In this sense it has been developed techniques for processing, conservation and storage of tissues, called homografts, which are obtained from cadaveric donors, in which still cellular viability is conserved. Unfortunately, of another hand, it is left the sadness to see frustrated the life of thousands of people who by violence or other accidental causes are death; although it increases the opportunities of relievement for great amount of patients who wait by biological implants derived of a donor; it is the ambivalence that exist between the opportunities to save lives and the social cost that represent the victims of the violence which they are offered like donors.