Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus

Abstract This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5-200 µg/ml (r > 0.999). The range of recoveries was 92.27-102.54%. The relative standard deviation values for intra- and inter-day precision studies were 4.30 and 3.78%, respectively. This assay can be readily utilized as quality control method for catechin in the dried extract of P. pseudocaryophyllus.

Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves

Abstract Spondias mombin L., Anacardiaceae, is a plant native of Brazil, where it is known as "cajá". In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated. The anti-inflammatory activity was evaluated using the in vivo model carrageenan-induced peritonitis in mice. The in vitro antioxidant potential as well the cytotoxicity against 3T3 fibroblast cells also were evaluated. Through High Performance Liquid Chromatography-diode array detector analysis, an analytic method was developed and validated. It allowed the identification and quantification of ellagic acid and chlorogenic acid in hydroethanolic extract of S. mombin leaves. This extract showed anti-inflammatory effect at 100, 200, 300 and 500 mg/kg, however, the ethyl acetate fraction, at 200 mg/kg, showed the highlighted results. Ellagic acid and chlorogenic acid (2.5, 5 and 10 mg/kg) also inhibited the leukocyte migration to the site of inflammation. The extract, fractions and compounds showed significant antioxidant potential when evaluated in different assays. The results shown in this work suggest the anti-inflammatory potential of the leaf extract of S. mombim on peritonitis model induced by carrageenan, it was also observed antioxidant properties associated with an absence of cytotoxicity in cell culture. Further in vivo studies are required to confirm the anti-inflammatories action of S. mombin and its possible anti-inflammatory mechanisms of action.

Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis

AbstractThe aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 × 4.6 mm, 5 μm). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.

Validación de un método por CLAR para la cuantificación de L-prolina en la tintura al 20 por ciento de Murraya paniculata L. Jack / Validation of a high performance liquid chromatography method for quantitation of L-proline in 20 percent tincture from Murraya paniculata L. Jack

INTRODUCCIÓN: la búsqueda de técnicas analíticas para el control de la calidad de los medicamentos constituye un aspecto de gran interés en el campo farmacéutico, más si van dirigidas al estudio del o los marcadores químicos de las plantas medicinales, sus extractos y fitomedicamentos. OBJETIVO: validar un método de cromatografía líquida de alta resolución (CLAR) para la determinación cuantitativa del aminoácido L-prolina como sustancia marcador en la tintura de Murraya paniculata L. Jack. MÉTODOS: en el método por CLAR, la separación se realizó en una columna C-18 (UP5ODB-150/046), se utilizó como fase móvil una mezcla de solución buffer fosfato, pH ajustado a 2,4 y acetonitrilo (70:30 v/v), con una velocidad de flujo de 0,6 mL/min, modo isocrático, con detección ultravioleta a 440 nm. El volumen de inyección de la muestra fue de 20 µL. El método fue validado según la categoría I, siguiendo las exigencias internacionales. RESULTADOS: la curva de calibración fue lineal en el rango de concentraciones ensayadas (30 a 375 µg/mL), se observó una buena precisión con coeficientes de variación menores del 2 por ciento. Los valores de recobrado estuvieron dentro de los límites establecidos para los métodos cromatográficos (98-102 por ciento). Se demostró la especificidad del método, al no presentarse interferencias de picos adicionales en la zona de elusión del compuesto de interés (L-prolina). CONCLUSIONES: el método analítico por CLAR, validado para la cuantificación del aminoácido L-prolina en la tintura de M. paniculata, demostró ser lineal, preciso, exacto y específico bajo las condiciones de estudio(AU)

INTRODUCTION: the search for analytical methods that may monitor the quality of drugs is an issue of great interest in the pharmaceutical field, even more if they are directed to studying chemical markers of medicinal plants, their extracts and phytomedicines. OBJECTIVE: to validate a high-resolution liquid chromatography (HPLC) method for the quantitative determination of the L-proline amino acid as a marker substance in Murraya paniculata L. Jack tincture. METHODS: in the HPLC, the separation was performed on a C-18 (UP5ODB-150/046) column, with a mixture of phosphate buffer solution, pH adjusted to 2.4 and acetonitrile (70:30 v/v) used as mobile phase, the flow rate was 0.6 mL/min, isocratic mode with UV detection set at 440 nm. The injection volume of the sample was 20 µL. The method was validated according to category I, following international requirements. RESULTS: the calibration curve was linear over the concentration range tested (30-375 mg/mL), good precision was observed with a variation coefficient less than 2 percent. Recovery values were within the limits for chromatographic methods (98-102 percent). The method was specific since there was no-interference by additional peaks in the elution zone of the compound in question (L-proline). CONCLUSIONS: the HPLC analytical method, validated for the quantification of L-proline amino acid in M. paniculata tincture, proved to be linear, precise, accurate and specific under the study conditions(AU)

Um marcador químico de fácil detecção para a própolis de Alecrim-do-Campo (Baccharis dracunculifolia) / An easily detectable chemical marker for the Baccharis dracunculifolia propolis

O presente trabalho apresenta um marcador químico (MQ) volátil, de fácil detecção por cromatografia gasosa, para a própolis do alecrim-do-campo (Baccharis dracunculifolia). Trata-se do composto volátil mais abundante no extrato em diclorometano de própolis verdes dessa planta, mas que aparece, também, em diferentes concentrações, em extratos de diclorometano de própolis marrom, preta, vermelha e amarela, provenientes de regiões que contêm Baccharis dracunculifolia. O MQ está presente no extrato dos ápices vegetativos de alecrim em concentração significativa, mas sua concentração na folha de alecrim é baixa. Própolis de regiões sem alecrim não possuem o MQ. Este composto foi isolado recentemente e se trata do 3-prenilcinamato de alila. Amostras comerciais de extratos etanólicos de própolis verdes foram analisadas e a de primeira qualidade, tipo exportação, apresentou maior concentração de MQ. Tal descoberta facilita o rápido controle de qualidade de extratos etanólicos de própolis verdes.

In the present work a volatile chemical marker (CM) for the Baccharis dracunculifolia (Bd) propolis is proposed, which is easily detectable by gas chromatography. It is the most abundant volatile compound in dichloromethane extracts of green propolis from this plant, but it appears also, in different concentrations, in dichloromethane extracts of brown, dark and red propolis from regions where Bd grows. The CM is present in significative concentration in the bud extract of Bd, in contrast to the leaf extract where its concentration is low. Propolis from regions without Bd does not contain the CM. This compound was recently isolated; it is the allyl 3-prenylcinnamate. Commercial samples of green propolis ethanol extract were analyzed and the first quality one (exportation standard) presented the highest concentration on CM. This finding makes easier the quality control of green propolis extracts sold at the market.