Exhaustive identification and characterization of Colletotrichum siamense and Colletotrichum fructicola as causative agents of circular leaf spot disease of rubber tree (Hevea brasiliensis) in India.

The rubber tree (Hevea brasiliensis) is one of the major domesticated crops planted commercially for the production of natural rubber (NR) worldwide. In recent years, rubber trees in the Southern states of India and other rubber-producing countries have experienced a severe leaf spot disease, characterized by the appearance of several brown circular spots in the initial stage, which later spread all over the lamina of fully matured leaves, leading to yellowing and defoliation. The causal organism of this Circular Leaf Spot (CLS) disease has not been conclusively identified in any previous studies. In this study, we collected infected leaf samples from various locations in the South Indian states. We aimed to identify the actual fungal pathogen that causes the CLS disease on rubber trees. Based on the morphological and molecular analysis of the most frequently isolated fungi from infected leaf samples were identified as Colletotrichum siamense and Colletotrichum fructicola. Pathogenicity tests also confirmed the involvement of isolated Colletotrichum spp. in the development of CLS disease. These findings provide valuable insights into understanding the CLS disease and its impact on rubber cultivation. To our knowledge, it is the first report of C. siamense and C. fructicola associated with CLS disease of rubber trees in India.

Infestation of Portulaca oleracea (Portulacaceae) plants by Neotuerta platensis (Lepidoptera: Noctuidae) larvae in Brazil: evaluations for five consecutive years / Infestação de plantas de Portulaca oleracea (Portulacaceae) por larvas de Neotuerta platensis (Lepidoptera: Noctuidae) no Brasil: avaliações por cinco anos consecutivos

The bioecology and infestation aspects of Neotuerta platensis Berg, 1882 (Lepidoptera: Noctuidae) on plants are poorly known. This insect fed on the leaves of common purslane, Portulaca oleracea L. (Portulacaceae) for two consecutive years, which triggered its study in the following five years in Januária, Minas Gerais State, Brazil. The objective of this work was to study the bioecology and infestation aspects of N. platensis on P. oleracea plants in the field and laboratory. The mean duration (± SD) of the egg, larva and pupa stages was 3.6 ± 0.89, 11.5 ± 2.81 and 10.7 ± 1.97 days, respectively. The mean numbers of egg masses and eggs per female (± SD) were 3.8 ± 1.16 and 891.6 ± 116.83, respectively. The percentage of infested plants was 59, 74, 0, 78 and 75% and the mean numbers of larvae per plant (± SD) ranged from 0.7 ± 0.45 to 1.6 ± 0.49 individuals, respectively from 2015 to 2019. Neotuerta platensis larvae infested P. oleracea plants in four out of five years evaluated.

Os aspectos de bioecologia e infestação de Neotuerta platensis Berg, 1882 (Lepidoptera: Noctuidae) em plantas são pouco conhecidos. Esse inseto se alimentou das folhas de beldroega comum, Portulaca oleracea L. (Portulacaceae) por dois anos consecutivos, o que desencadeou seu estudo nos cinco anos seguintes em Januária, Minas Gerais, Brasil. O objetivo deste trabalho foi estudar a bioecologia e os aspectos da infestação de N. platensis em plantas de P. oleracea em campo e laboratório. A duração média (± DP) dos estágios de ovo, larva e pupa foi de 3,6 ± 0,89, 11,5 ± 2,81 e 10,7 ± 1,97 dias, respectivamente. Os números médios de posturas e ovos por fêmea (± DP) foram de 3,8 ± 1,16 e 891,6 ± 116,83, respectivamente. A porcentagem de plantas infestadas foi de 59, 74, 0, 78 e 75% e os números médios de larvas por planta (± DP) variaram de 0,7 ± 0,45 a 1,6 ± 0,49 indivíduos, respectivamente de 2015 a 2019. Larvas de N. platensis infestaram plantas de P. oleracea em quatro dos cinco anos avaliados.

Infestation of Portulaca oleracea (Portulacaceae) plants by Neotuerta platensis (Lepidoptera: Noctuidae) larvae in Brazil: evaluations for five consecutive years

Abstract The bioecology and infestation aspects of Neotuerta platensis Berg, 1882 (Lepidoptera: Noctuidae) on plants are poorly known. This insect fed on the leaves of common purslane, Portulaca oleracea L. (Portulacaceae) for two consecutive years, which triggered its study in the following five years in Januária, Minas Gerais State, Brazil. The objective of this work was to study the bioecology and infestation aspects of N. platensis on P. oleracea plants in the field and laboratory. The mean duration (± SD) of the egg, larva and pupa stages was 3.6 ± 0.89, 11.5 ± 2.81 and 10.7 ± 1.97 days, respectively. The mean numbers of egg masses and eggs per female (± SD) were 3.8 ± 1.16 and 891.6 ± 116.83, respectively. The percentage of infested plants was 59, 74, 0, 78 and 75% and the mean numbers of larvae per plant (± SD) ranged from 0.7 ± 0.45 to 1.6 ± 0.49 individuals, respectively from 2015 to 2019. Neotuerta platensis larvae infested P. oleracea plants in four out of five years evaluated.

Resumo Os aspectos de bioecologia e infestação de Neotuerta platensis Berg, 1882 (Lepidoptera: Noctuidae) em plantas são pouco conhecidos. Esse inseto se alimentou das folhas de beldroega comum, Portulaca oleracea L. (Portulacaceae) por dois anos consecutivos, o que desencadeou seu estudo nos cinco anos seguintes em Januária, Minas Gerais, Brasil. O objetivo deste trabalho foi estudar a bioecologia e os aspectos da infestação de N. platensis em plantas de P. oleracea em campo e laboratório. A duração média (± DP) dos estágios de ovo, larva e pupa foi de 3,6 ± 0,89, 11,5 ± 2,81 e 10,7 ± 1,97 dias, respectivamente. Os números médios de posturas e ovos por fêmea (± DP) foram de 3,8 ± 1,16 e 891,6 ± 116,83, respectivamente. A porcentagem de plantas infestadas foi de 59, 74, 0, 78 e 75% e os números médios de larvas por planta (± DP) variaram de 0,7 ± 0,45 a 1,6 ± 0,49 indivíduos, respectivamente de 2015 a 2019. Larvas de N. platensis infestaram plantas de P. oleracea em quatro dos cinco anos avaliados.

INFLUENCIA DE LA HERBIVORÍA Y LA APERTURA DE CLAROS SOBRE EL CRECIMIENTO TOTAL DE Brosimum alicastrum (Moraceae) / Influence of herbivory and gap-openness on whole-plant growth of Brosimum alicastrum (Moraceae)

RESUMEN El crecimiento compensatorio y las defensas vegetales son respuestas para lidiar con la herbivoría y la luz. El estudio de la influencia de los componentes morfo-fisiológicos, de la asignación de biomasa y de la defensa vegetal podría arrojar información que ayude a entender estas respuestas. Se evaluaron los efectos de la herbivoría y la apertura de claros sobre el crecimiento, los componentes morfo-fisiológicos, la asignación de biomasa y las defensas químicas y físicas en plántulas de Brosimum alicastrum en una selva mediana subcaducifolia. Se utilizó un diseño anidado con sitios de sotobosque y claros (n = 3 en cada uno) y dentro de ellos plántulas sometidas a tres tratamientos de herbivoría: control (n = 10), simulada (n = 10) y natural (n = 10). Se registró a lo largo de seis meses la influencia de la herbivoría sobre el crecimiento (biomasa, área foliar, altura, diámetro y producción de hojas), la producción de fenoles y la dureza foliar. Se halló un crecimiento compensatorio (todas las variables de crecimiento) con la herbivoría natural, subcompensatorio (biomasa, altura y diámetro) con la simulada, y sobrecompensatorio (producción de hojas) con la herbivoría natural sólo en claros. Los componentes morfológicos como el cociente del área foliar (LAR) y el área foliar específica (SLA) fueron mayores bajo claros y el fisiológico, como la tasa de asimilación neta (NAR), en sotobosque. La proporción de biomasa en sotobosque fue mayor hacia hojas y tallos y en claros más alta hacia raíces. La herbivoría fue mayor en claros y los fenoles foliares en sotobosque. No se encontró una relación (compromiso) entre las defensas y el crecimiento, sólo una tendencia negativa con los fenoles bajo condiciones limitantes de recursos, como ocurre en el sotobosque.

ABSTRACT Compensatory plant growth and plant defense are responses to deal with herbivory and light availability. The research focused on compensatory growth responses explained through the influence of morphological and physiological components of plant growth, allocation mass, and plant defense could clarify such process. We studied the effects of herbivory and gap-openness on compensatory growth responses, morphological and physiological components, allocation mass, and chemical defenses in seedlings of Brosimum alicastrum in a subhumid tropical forest. We used a nested design with understory and gap-openness site (n = 3 each) within each replicated site seedling belong to three herbivory treatments (n = 10 each): control, simulated, and natural; and along six months we studied the influence of leaf herbivory on plant growth (mass, leaf area, height, diameter, and leaf production) and production of phenols and leaf toughness. We recorded compensatory growth responses (all plant growth variables) with natural herbivory, subcompensatory (mass, height, and diameter) with simulated and, overcompensatory (leaf production) with natural herbivory although only under gap-openness. We found a higher leaf area ratio (LAR) and specific leaf area (SLA) (both morphological components) under gap-openness and high values of net assimilation rate (NAR) on understory. The mass proportion was highest in leaves and stems on the understory and higher in roots under gap-openness. Leafherbivory was highest under gap-openness while the phenols of the leaves on understory. We did not find a relationship (trade-off) between defense with plant growth, but it was possible to underline a negative pattern of the leaf phenols under limiting resources environment such as understory.

Highest Defoliation Tolerance in Amaranthus cruentus Plants at Panicle Development Is Associated With Sugar Starvation Responses.

Defoliation tolerance (DT) in Amaranthus cruentus is known to reach its apex at the panicle emergence (PE) phase and to decline to minimal levels at flowering (FL). In this study, defoliation-induced changes were recorded in the content of non-structural carbohydrates and raffinose family oligosaccharides (RFOs), and in the expression and/or activity of sugar starvation response-associated genes in plants defoliated at different vegetative and reproductive stages. This strategy identified sugar-starvation-related factors that explained the opposite DT observed at these key developmental stages. Peak DT at PE was associated with increased cytosolic invertase (CI) activity in all organs and with the extensive induction of various class II trehalose-phosphate synthase (TPS) genes. Contrariwise, least DT at FL coincided with a sharp depletion of starch reserves and with sucrose (Suc) accumulation, in leaves and stems, the latter of which was consistent with very low levels of CI and vacuolar invertase activities that were not further modified by defoliation. Increased Suc suggested growth-inhibiting conditions associated with altered cytosolic Suc-to-hexose ratios in plants defoliated at FL. Augmented cell wall invertase activity in leaves and roots, probably acting in a regulatory rather than hydrolytic role, was also associated with minimal DT observed at FL. The widespread contrast in gene expression patterns in panicles also matched the opposite DT observed at PE and FL. These results reinforce the concept that a localized sugar starvation response caused by C partitioning is crucial for DT in grain amaranth.

Impact of Diamondback Moth Density and Infestation Timing on Broccoli Yield.

The diamondback moth (DBM), Plutella xylostella (L.) (Lep.: Plutellidae), is an important pest of broccoli (Brassica oleracea L. var. italica Plenck). Few studies have focused on the real DBM impact on broccoli yield. We performed greenhouse studies to assess the effect of DBM densities and infestation timing (at pre-heading, heading, or during the entire cycle) on broccoli head weight. Polynomial trend analysis revealed a downward linear response of head weight to DBM densities, indicating that broccoli is susceptible to DBM attack. As for the infestation timing, infestations during the pre-heading stage significantly impacted head weight, whereas the same did not occur for infestations during the heading stage. DBM density did not affect plant height or total leaves but was upward-related to plant defoliation, and head weight correlated negatively with plant defoliation. These findings indicate that pre-heading is the determining stage for head weight loss of plants under DBM attack. In order to prevent quantitative losses, efforts for DBM management should be focused on this particular stage.

How much leaf area do insects eat? A data set of insect herbivory sampled globally with a standardized protocol.

Herbivory is ubiquitous. Despite being a potential driver of plant distribution and performance, herbivory remains largely undocumented. Some early attempts have been made to review, globally, how much leaf area is removed through insect feeding. Kozlov et al., in one of the most comprehensive reviews regarding global patterns of herbivory, have compiled published studies regarding foliar removal and sampled data on global herbivory levels using a standardized protocol. However, in the review by Kozlov et al., only 15 sampling sites, comprising 33 plant species, were evaluated in tropical areas around the globe. In Brazil, which ranks first in terms of plant biodiversity, with a total of 46,097 species, almost half (43%) being endemic, a single data point was sampled, covering only two plant species. In an attempt to increase knowledge regarding herbivory in tropical plant species and to provide the raw data needed to test general hypotheses related to plant-herbivore interactions across large spatial scales, we proposed a joint, collaborative network to evaluate tropical herbivory. This network allowed us to update and expand the data on insect herbivory in tropical and temperate plant species. Our data set, collected with a standardized protocol, covers 45 sampling sites from nine countries and includes leaf herbivory measurements of 57,239 leaves from 209 species of vascular plants belonging to 65 families from tropical and temperate regions. They expand previous data sets by including a total of 32 sampling sites from tropical areas around the globe, comprising 152 species, 146 of them being sampled in Brazil. For temperate areas, it includes 13 sampling sites, comprising 59 species. Thus, when compared to the most recent comprehensive review of insect herbivory (Kozlov et al.), our data set has increased the base of available data for the tropical plants more than 460% (from 33 to 152 species) and the Brazilian sampling was increased 7,300% (from 2 to 146 species). Data on precise levels of herbivory are presented for more than 57,000 leaves worldwide. There are no copyright restrictions. Please cite this paper when using the current data in publications; the authors request to be informed how the data is used in the publications.

Effects of Soybean Plant Population on Yield Loss From Defoliation.

Plant densities in Mid-South U.S. soybean, Glycine max (L.) Merr., fields can vary greatly due to a wide range of factors, although soybean yields are generally insensitive to variations in density. Currently, it is unknown if yield loss from insect-related defoliation varies across different soybean stand densities. Soybean was planted in Starkville and Stoneville, MS, in 2016 and 2017 at five seeding rates ranging from 123,500 to 420,070 seed/ha in 74,130 seed/ha increments. Each seeding rate contained a nondefoliated plot and a plot that was defoliated 67% at the R1 growth stage. Defoliated plants had a greater leaf expansion rate from R1 to R3 than nondefoliated plants. Defoliation reduced yield where plant densities were <192,800 plants/ha, but greater densities were not affected. Reduced yield in defoliated plots when compared with nondefoliated plots at equivalent R3 leaf area index values indicated that some resources were used to replace the removed leaf area instead of seed production. These results suggest that fields with substandard plant densities might benefit from a reduced treatment threshold for defoliating pests.

Low-Intensity, High-Frequency Grazing Strategy Increases Herbage Production and Beef Cattle Performance on Sorghum Pastures.

We assessed the effects of high-intensity and low-frequency (HILF) vs. low-intensity and high-frequency (LIHF) grazing on herbage production and performance of beef cattle grazing sorghum pastures. The experimental design was a complete randomized block with two treatments and four replicates (paddocks), carried out in 2014/15. The management target of 50 and 30 cm for pre- and post-grazing, respectively, a LIHF grazing management strategy oriented to maximize beef cattle herbage intake per unit time, was compared with a HILF grazing management strategy of 80 and 20 cm for pre- and post-grazing, respectively, aiming to maximize herbage accumulation and harvest efficiency. Sixteen Brangus steers of 15-month-old and 265 ± 21 kg of live weight (LW) were randomly distributed to paddocks (experimental units). The LIHF resulted in shorter rest periods when compared with the HILF. The greater leaf lamina mass in LIHF allowed greater sward light interception at post-grazing, resulting in greater total herbage production than HILF (7581 and 4154 kg DM/ha, respectively). The average daily gain (ADG) of steers was greater for the LIHF than for the HILF treatment (0.950 and 0.702 kg/animal, respectively); however, even with a greater stocking rate in the HILF, there was no difference for LW gain per ha, with an average of 4 kg LW/ha/day. Our findings demonstrated that the LIHF strategy that is based on offering to the animals an optimal sward structure to favor the maximum herbage intake rate fosters greater herbage production, harvesting efficiency, and ADG without compromising LW gain per area of beef steers, despite the lower herbage harvested per stocking cycle.

Are economic thresholds for IPM decisions the same for low LAI soybean cultivars in Brazil?

BACKGROUND: Economic thresholds (ETs) are well-established for defoliation of soybean, Glycine max, and have been updated for many of the newer cultivars; however, there is increasing grower adoption of cultivars with a reduced leaf area index (LAI). It is of theoretical and practical interest to determine low LAI cultivar tolerance to defoliation. We conducted experiments during two consecutive crop seasons (2017/2018 and 2018/2019) using three soybean cultivars (NS 5959 IPRO, NS 5445 IPRO, and DON MARIO 5.8i) and three defoliation levels (0%, 16.7%, and 33.3%) to evaluate the tolerance of reduced LAI soybean cultivars under different defoliation levels. RESULTS: We observed differences among cultivar's LAI during plant development during both years. Soybean LAI was reduced with increasing defoliation intensity. Tested continuous defoliation levels from plant development stages of V2 to R6 reduced the weight of 1000 seeds and yield but did not impact oil or protein content. CONCLUSIONS: Despite our findings that current ET for defoliators in soybean (30% defoliation during vegetative stage and 15% defoliation during reproductive stage) are valid, it is important to consider that continuous defoliation injury impacts the capacity of the plant to respond to injury and must be further evaluated for ET refinement in future research. © 2020 Society of Chemical Industry.

In Vitro Fermentation Patterns and Methane Output of Perennial Ryegrass Differing in Water-Soluble Carbohydrate and Nitrogen Concentrations.

The objective of this study was to determine the effect of perennial ryegrass (PRG) forages differing in their concentration of water-soluble carbohydrates (WSC) and crude protein (CP), and collected in spring and autumn, on in vitro rumen fermentation variables, nitrogen (N) metabolism indicators and methane (CH4) output, using a batch culture system. Two contrasting PRG pastures, sampled both in autumn and spring, were used: high (HS) and low (LS) sugar pastures with WSC concentrations of 322 and 343 g/kg for HS (autumn and spring), and 224 and 293 g/kg for LS in autumn and spring, respectively. Duplicates were incubated for 24 h with rumen inocula in three different days (blocks). Headspace gas pressure was measured at 2, 3, 4, 5, 6, 8, 10, 12, 18, and 24 h, and CH4 concentration was determined. The supernatants were analysed for individual volatile fatty acids (VFA) concentrations, and NH3-N. The solid residue was analysed for total N and neutral detergent insoluble N. Another set of duplicates was incubated for 4 h for VFA and NH3-N determination. The HS produced more gas (218 vs. 204 mL/g OM), tended to increase total VFA production (52.0 mM vs. 49.5 mM at 24 h), reduced the acetate:propionate ratio (2.52 vs. 3.20 at 4 h and 2.85 vs. 3.19 at 24 h) and CH4 production relative to total gas production (15.6 vs. 16.8 mL/100 mL) and, improved N use efficiency (22.1 vs. 20.9). The contrasting chemical composition modified in vitro rumen fermentation tending to increase total VFA production, reduce the acetate:propionate ratio and CH4 concentration, and improve N use efficiency through lower rumen NH3-N.

The morphogenesis of marandu palisadegrass at fixed or variable heights in different seasons of the year / Morfogênese do capim-marandu em alturas fixas ou variáveis ​​em diferentes estações do ano

Height variations in a sward over the year may be efficient for increasing plant growth, compared with maintenance of the sward at a constant height. Thus, this experiment was conducted from February 2013 to May 2014 to characterise the development of Urochloa brizantha syn. Brachiaria brizantha cv.Marandu (marandu palisadegrass), managed under three defoliation strategies: constant height (30 cm during the entire experimental period), increasing height (15 cm in the winter, 30 cm in the spring, and 45 cm in the summer), and decreasing height (45 cm in the winter, 30 cm in the spring, and 15 cm in the summer). The experimental design was completely randomised, in a split-plot arrangement, with four replicates. Phyllochron was higher in the winter, but leaf and stem elongation rates and the number of live leaves per tiller were lower compared with the spring and summer. In the summer, the swards under increasing height displayed a higher phyllochron than those under decreasing and constant height. When comparing seasons, the highest leaf senescence rate occurred in spring. In the winter, the sward with increasing height had shorter leaves and stems, in contrast to the summer. The sward under decreasing height showed a high stem elongation rate in the spring. Marandu palisadegrass has great flexibility in terms of defoliation management and a typical seasonal development pattern. Modifying the sward height results in a gradual change in the development of marandu palisadegrass and generates residual effects on the subsequent season.

As variações de altura em um pasto ao longo do ano podem ser eficientes em aumentar o crescimento das plantas, em comparação com a manutenção do pasto a uma altura constante. Assim, este experimento foi conduzido de fevereiro de 2013 a maio de 2014 para caracterizar o desenvolvimento de Urochloa brizantha syn. Brachiaria brizantha cv. Marandu (capim-marandu) manejado com três estratégias de desfolhação: altura constante (30 cm durante todo o período experimental), altura crescente (15 cm no inverno, 30 cm na primavera e 45 cm no verão) e altura decrescente (45 cm no inverno, 30 cm na primavera e 15 cm no verão). O delineamento experimental foi inteiramente casualizado, em esquema de parcelas subdivididas, com quatro repetições. O filocrono foi maior no inverno, mas as taxas de alongamento de folhas e caules e o número de folhas vivas por perfilho foram menores em comparação com a primavera e o verão. No verão, o relvado em altura crescente apresentou filocrono maior do que em altura decrescente e constante. Entre as estações, a maior taxa de senescência foliar ocorreu na primavera. No inverno, o pasto com o aumento da altura apresentava folhas e caules mais curtos, ao contrário do verão. O relvado em altura decrescente apresentou alta taxa de alongamento na primavera. O capim-marandu possui grande flexibilidade em termos de manejo de desfolha e um padrão típico de desenvolvimento sazonal. A modificação da altura do pasto resulta em uma mudança gradual no desenvolvimento do capim-marandu e gera efeitos residuais na estação subsequente.

Defoliation dynamics of Brachiaria brizantha pastures with distinct structural characteristics / Dinâmica de desfolhação em pastos de Brachiaria brizantha com características estruturais distintas

Although belonging to the same species, different Brachiaria brizantha varieties used for pasture vary significantly in their morphological structure. Therefore, this study starts from the central hypothesis that the defoliation dynamics during the period of occupation by animals in pastures formed by Brachiaria brizantha under rotational stocking varies among cultivars during the year. The experimental design used was randomized blocks with three treatments and three repetitions, with the cultivars consisting of Brachiaria brizantha cv. Xaraés, BRS Paiaguás, and Ecótipo B4. The defoliation patterns during the grazing periods were estimated using the marked tiller technique. Despite alterations in the tiller and forage mass components in the wet and dry seasons, no variations were observed in the tiller defoliation dynamics and the respective structural components. In the wet season, the area grazed only once was greater for BRS Paiaguás compared to the other two cultivars, but the other areas representing the grazing horizons were equally exploited among the grass varieties during the year. Therefore, based on the defoliation dynamics of pastures in Brazil's tropical climate, it is possible to suggest that pastures formed by grasses with different structures and growth habits can be submitted to similar management strategies without impairing the forage canopy exploitation by the animals.

Apesar de pertencerem à mesma espécie, existem diversas variedades disponíveis no mercado de pastos de Brachiaria brizantha, os quais variam significativamente na estrutura. Assim, este trabalho partiu da hipótese central que a dinâmica de desfolhação durante o período de ocupação dos animais em pastos de Brachiaria brizantha, sob lotação rotativa, varia entre cultivares ao longo do ano. O delineamento experimental utilizado foi de blocos casualizados com três tratamentos e três repetições constituídos pelas Brachiaria brizantha cv. Xaraés, BRS Paiaguás e o ecótipo B4. Os padrões de desfolhação, durante o rebaixamento dos pastos, foram estimados utilizando a técnica de perfilhos marcados. Apesar de alterações nos componentes do perfilho e massa de forragem, na época das águas e secas, não foi observado variações na dinâmica de desfolhação de perfilho e seus respectivos componentes estruturais. No período das águas a área pastejada apenas uma vez, foi maior na BRS Paiaguás quando comparado aos demais pastos, porém as demais áreas que equivalem aos horizontes de pastejo foram igualmente exploradas entre as gramíneas durante o ano. Portanto, baseado na dinâmica de desfolhação em pastos de clima tropical, é possível sugerir que pastos formados com gramíneas com diferentes estruturas e hábitos de crescimento podem ser submetidos a semelhantes estratégias de manejo sem prejudicar o processo de exploração do dossel forrageiro pelo animal.

Megathyrsus Maximus cv. Massai at different cutting frequencies / Megathyrsus Maximus cv. Massai submetido a frequências de corte

We evaluated the growth dynamics and forage production of Massai grass subjected to different cutting frequencies during the rainy season, with the aim to determine the most suitable cutting frequency to increase grass productivity. A randomized block design with four treatments (four cutting frequencies, 24, 32, 48, and 96 days) was used. The morphological characteristics were evaluated weekly in seven random tillers. Leaf appearance rate, phyllochron value, foliar elongation rate, leaf senescence rate, and stem elongation rate were influenced (p < 0.05) by the cutting frequency, while no effect was found for leaf life span. The number of live leaves per tiller, mean length of leaf blades, population density of tillers (DPP), and sheath length were influenced by the cutting frequency, along with plant height, dry mass of harvested leaf blade, dry mass of harvested stem, dry mass of harvested dead material, and leaf/stem ratio, while there was no effect on dry mass of forage harvested. The rates of leaf blade accumulation, stem accumulation, forage loss, and forage production were not affected by cutting frequency. A cutting frequency of 24 days resulted in the highest productivity values of Massai grass during the rainy season.(AU)

Objetivou-se neste trabalho avaliar a dinâmica de crescimento e produção de forragem do capim Massai submetido as frequências de desfolhação, durante o período chuvoso, com intuito de elucidar a melhor frequência de desfolhação no manejo do pastejo, para garantir maior produtividade do capim. Foi utilizado o delineamento experimental em blocos casualizado, com quatro tratamentos (quatro frequências de desfolhação do dossel, sendo 24; 32; 48 e 96 dias de intervalo de corte). As características morfogênicas foram avaliadas após o corte de uniformização, em sete perfilhos ao acaso por repetição. Estas avaliações foram feitas semanalmente. A taxa de aparecimento foliar, o filocrono, a taxa de alongamento foliar, a taxa de senescência foliar e a taxa de alongamento do colmo foram influenciados (p < 0,05) pelas frequências de corte do capim Massai, não havendo efeito (p > 0,05) na duração de vida da folha. O número de folhas vivas por perfilho, o comprimento médio de lâminas foliares, a densidade populacional de perfilhos (DPP) e o comprimento da bainha foram influenciados pelas frequências de corte do capim Massai. A altura da planta, a massa seca de lâmina foliar colhida, a massa seca do colmo colhida, a massa seca de material morto colhida e a relação folha/colmo foram influenciados pelas frequências de corte, não havendo resposta na massa seca de forragem colhida. As taxas de acúmulo de lâmina foliar, o acúmulo de colmo, a perda de forragem e a produção bruta de forragem não foram influenciadas pelas frequências de corte. A frequência de desfolhação no período de 24 dias, apresentou os melhores valores de produtividade do capim Massai durante o período chuvoso.(AU)

Megathyrsus Maximus cv. Massai at different cutting frequencies / Megathyrsus Maximus cv. Massai submetido a frequências de corte

We evaluated the growth dynamics and forage production of Massai grass subjected to different cutting frequencies during the rainy season, with the aim to determine the most suitable cutting frequency to increase grass productivity. A randomized block design with four treatments (four cutting frequencies, 24, 32, 48, and 96 days) was used. The morphological characteristics were evaluated weekly in seven random tillers. Leaf appearance rate, phyllochron value, foliar elongation rate, leaf senescence rate, and stem elongation rate were influenced (p < 0.05) by the cutting frequency, while no effect was found for leaf life span. The number of live leaves per tiller, mean length of leaf blades, population density of tillers (DPP), and sheath length were influenced by the cutting frequency, along with plant height, dry mass of harvested leaf blade, dry mass of harvested stem, dry mass of harvested dead material, and leaf/stem ratio, while there was no effect on dry mass of forage harvested. The rates of leaf blade accumulation, stem accumulation, forage loss, and forage production were not affected by cutting frequency. A cutting frequency of 24 days resulted in the highest productivity values of Massai grass during the rainy season.

Objetivou-se neste trabalho avaliar a dinâmica de crescimento e produção de forragem do capim Massai submetido as frequências de desfolhação, durante o período chuvoso, com intuito de elucidar a melhor frequência de desfolhação no manejo do pastejo, para garantir maior produtividade do capim. Foi utilizado o delineamento experimental em blocos casualizado, com quatro tratamentos (quatro frequências de desfolhação do dossel, sendo 24; 32; 48 e 96 dias de intervalo de corte). As características morfogênicas foram avaliadas após o corte de uniformização, em sete perfilhos ao acaso por repetição. Estas avaliações foram feitas semanalmente. A taxa de aparecimento foliar, o filocrono, a taxa de alongamento foliar, a taxa de senescência foliar e a taxa de alongamento do colmo foram influenciados (p 0,05) na duração de vida da folha. O número de folhas vivas por perfilho, o comprimento médio de lâminas foliares, a densidade populacional de perfilhos (DPP) e o comprimento da bainha foram influenciados pelas frequências de corte do capim Massai. A altura da planta, a massa seca de lâmina foliar colhida, a massa seca do colmo colhida, a massa seca de material morto colhida e a relação folha/colmo foram influenciados pelas frequências de corte, não havendo resposta na massa seca de forragem colhida. As taxas de acúmulo de lâmina foliar, o acúmulo de colmo, a perda de forragem e a produção bruta de forragem não foram influenciadas pelas frequências de corte. A frequência de desfolhação no período de 24 dias, apresentou os melhores valores de produtividade do capim Massai durante o período chuvoso.

Productive and structural responses of Urochloa brizantha cv. piatã subjected to management strategies / Respostas produtivas e estruturais do Urochloa brizantha cv. piatã submetido a estratégias de manejo

This experiment was carried out to evaluate management strategies for Piatã grass grown in the soil-climatic conditions of the south region of Mato Grosso State, Brazil. The treatments corresponded to four management strategies determined by the following pre- and post-harvest heights: 30/10, 30/15, 35/20 and 40/15. A completely randomized design with five replications was employed totaling twenty experimental units. The highest number of harvests was observed in the plots subjected to strategies 35/20 and 30/15, which allowed for two additional harvest cycles when compared with strategies 40/15 and 30/10. The strategies that provided plants with the greatest pre-harvest heights, 40/15 and 35/20, resulted in lower leaf elongation rates, stem elongation rates, and leaf senescence. Because of the lower post-harvest height, strategy 30/10 resulted in the largest tiller population, an average of 829 tillers m2. The highest herbage accumulation value, 18.600 kg ha-1 DM, was observed with management strategies 40/15 and 30/15. The management of Piatã grass based on the harvest target of 30/15 associates elevated herbage production with a favorable pasture structure for grazing.(AU)

Este experimento foi conduzido com o objetivo de avaliar as estratégias de manejo do capim Piatã cultivado nas condições solo-clima da região sul do Estado do Mato Grosso. Os tratamentos corresponderam a quatro estratégias de manejo determinadas pelas seguintes alturas pré e pós-colheita: 30/10, 30/15, 35/20 e 40/15. Utilizou-se um delineamento inteiramente casualizado com cinco repetições, totalizando vinte unidades experimentais. O maior número de colheitas foi observado nas parcelas submetidas às estratégias 35/20 e 30/15, o que permitiu dois ciclos adicionais de colheita quando comparados com as estratégias 40/15 e 30/10. As estratégias que proporcionaram às plantas as maiores alturas pré-colheita, 40/15 e 35/20, resultaram em menores taxas de alongamento foliar, taxas de alongamento do colmo e senescência foliar. Devido à menor altura pós-colheita, a estratégia 30/10 resultou na maior população de perfilhos, uma média de 829 perfilhos m2. O maior valor de acúmulo de forragem, 18.600 kg ha-1 de MS, foi observado com as estratégias de manejo 40/15 e 30/15. O manejo do capim Piatã baseado na meta de colheita de 30/15 associa a produção elevada de forragem a uma estrutura de pasto favorável ao pastejo.(AU)

Productive and structural responses of Urochloa brizantha cv. piatã subjected to management strategies / Respostas produtivas e estruturais do Urochloa brizantha cv. piatã submetido a estratégias de manejo

This experiment was carried out to evaluate management strategies for Piatã grass grown in the soil-climatic conditions of the south region of Mato Grosso State, Brazil. The treatments corresponded to four management strategies determined by the following pre- and post-harvest heights: 30/10, 30/15, 35/20 and 40/15. A completely randomized design with five replications was employed totaling twenty experimental units. The highest number of harvests was observed in the plots subjected to strategies 35/20 and 30/15, which allowed for two additional harvest cycles when compared with strategies 40/15 and 30/10. The strategies that provided plants with the greatest pre-harvest heights, 40/15 and 35/20, resulted in lower leaf elongation rates, stem elongation rates, and leaf senescence. Because of the lower post-harvest height, strategy 30/10 resulted in the largest tiller population, an average of 829 tillers m2. The highest herbage accumulation value, 18.600 kg ha-1 DM, was observed with management strategies 40/15 and 30/15. The management of Piatã grass based on the harvest target of 30/15 associates elevated herbage production with a favorable pasture structure for grazing.

Este experimento foi conduzido com o objetivo de avaliar as estratégias de manejo do capim Piatã cultivado nas condições solo-clima da região sul do Estado do Mato Grosso. Os tratamentos corresponderam a quatro estratégias de manejo determinadas pelas seguintes alturas pré e pós-colheita: 30/10, 30/15, 35/20 e 40/15. Utilizou-se um delineamento inteiramente casualizado com cinco repetições, totalizando vinte unidades experimentais. O maior número de colheitas foi observado nas parcelas submetidas às estratégias 35/20 e 30/15, o que permitiu dois ciclos adicionais de colheita quando comparados com as estratégias 40/15 e 30/10. As estratégias que proporcionaram às plantas as maiores alturas pré-colheita, 40/15 e 35/20, resultaram em menores taxas de alongamento foliar, taxas de alongamento do colmo e senescência foliar. Devido à menor altura pós-colheita, a estratégia 30/10 resultou na maior população de perfilhos, uma média de 829 perfilhos m2. O maior valor de acúmulo de forragem, 18.600 kg ha-1 de MS, foi observado com as estratégias de manejo 40/15 e 30/15. O manejo do capim Piatã baseado na meta de colheita de 30/15 associa a produção elevada de forragem a uma estrutura de pasto favorável ao pastejo.

Grazing Preference of Dairy Cows and Pasture Productivity for Different Cultivars of Perennial Ryegrass under Contrasting Managements.

The objective of this study was to evaluate the pasture performance of different cultivars of perennial ryegrass, two "high sugar" and two standard cultivars, under two contrasting agronomic managements (aimed at either decreasing or increasing water soluble carbohydrates concentration), and their effects on the grazing preference of dairy cows. Eight treatments arising from the factorial combination of four cultivars and two managements were randomly applied to 31-m2 plots in three blocks. Pasture dry matter production and growth rate were measured for one year. Three grazing assessments were performed to establish the grazing preferences of six dairy cows in spring, summer and autumn. High sugar cultivars produced less dry matter per hectare than the standard cultivars. Cows consumed more grass and harvested a greater proportion of the pasture under the agronomic management aimed at decreasing sugar concentration, i.e., with a greater nitrogen fertilization rate and under a more frequent defoliation regime, which could be explained by the greater crude protein concentration achieved under this management. The results suggest that the genetic selection for greater levels of sugars was at the expense of herbage yield, and that cows preferred to graze herbage with a greater crude protein level instead of a greater sugar concentration.

Herbivore resistance in congeneric and sympatric Nothofagus species is not related to leaf habit.

PREMISE: Two fundamental hypotheses on herbivore resistance and leaf habit are the resource availability hypothesis (RAH) and the carbon-nutrient balance hypothesis (CNBH). The RAH predicts higher constitutive resistance by evergreens, and the CNBH predicts higher induced resistance by deciduous species. Although support for these hypotheses is mixed, they have rarely been examined in congeneric species. METHODS: We compared leaf constitutive and induced resistance (as leaf polyphenol and tannin concentrations, and as damage level in non-choice experiments) and leaf traits associated with herbivory of coexisting Nothofagus species using (1) a defoliation experiment and (2) natural defoliation caused by an outbreak of a common defoliator of Nothofagus species. RESULTS: In the defoliation experiment, polyphenol and tannin concentrations were similar between deciduous and evergreen species; regardless of leaf habit, polyphenols increased in response to defoliation. In the natural defoliation survey, N. pumilio (deciduous) had significantly higher herbivory, lower carbon/nitrogen ratio and leaf mass per area, and higher nitrogen and phosphorus concentrations than N. betuloides (evergreen); N. antarctica (deciduous) had intermediate values. Polyphenol concentrations and herbivore resistance indicated by the non-choice experiment were lower in N. pumilio than in N. antarctica and N. betuloides, which had similar values. CONCLUSIONS: Higher herbivory in N. pumilio was associated with a higher nutritional value and a lower level of leaf carbon-based defenses compared to both the evergreen and the other deciduous species, indicating that herbivore resistance in Nothofagus species cannot be attributed to only leaf habit as predicted by the RAH or CNBH.

Effect of post-grazing residual leaf blade-length on the regrowth vigor of Piatã grass grazed under intermittent stocking / Efeito do comprimento residual de lâmina foliar pós-pastejo no vigor de rebrotação de capim Piatã pastejado sob lotação intermitente

The objective of this study was to evaluate the effect of post-grazing residual leaf blades (RLB) of different lengths on the morphogenic and structural characteristics of Urochloa brizantha 'Piatã' grazed upon by beef cattle. A completely randomized design was used, with three RLB lengths (2, 4, and 6 cm) and two paddocks as replicates over two grazing seasons (wet and wet-dry transition). To monitor the weekly forage growth, two representative clumps and 48 tillers per treatment were marked after grazing. The procedure continued until the plants reached the target pre-grazing height of 35-40 cm. Morphogenic characteristics were not affected by the treatments or seasons, with minor changes observed in mean sheath length (MSL) within season and tiller population density (TPD). The MSL reached its peak value of 210.37 mm during the wet season relative to 175.89 mm during the wet-dry transition. The TPD reached its lowest value (680 tillers m <->2) at the highest defoliation intensity (2 cm) relative to that at the lowest defoliation intensity (720 tillers m <->2). The different RLB lengths modified canopy height variably; canopy height was the highest with the 6 cm leaves (24.31 cm), but did not vary between the 2 cm and 4 cm leaves. The post-grazing leaf area index and interception of photosynthetically active radiation were modified by season. The highest defoliation intensity (2 cm) increased the rest period by seven days when compared with the lowest defoliation intensity (6 cm).(AU)

Objetivou-se avaliar o efeito de diferentes comprimentos de lâmina foliar residual (RLF) pós-pastejo sobre as respostas morfogênicas e estruturais do capim Urochloa brizantha cv. Piatã pastejado por bovinos de corte. O delineamento experimental utilizado foi o inteiramente casualizado, com três RLF (2, 4 e 6 cm) e duas repetições de piquetes, ao longo de duas estações (águas e transição águas-seca). Após o pastejo dos bovinos eram marcadas duas touceiras representativas, e 48 perfilhos por tratamento, para o acompanhamento semanal do crescimento forrageiro, durante o período necessário para as plantas atingirem as metas de alturas pré-pastejo determinada entre 35 e 40 cm. As características morfogênicas não diferenciaram entre os tratamentos e estações, que promoveu pouca alteração nas características estruturais, apenas comprimento médio de bainha (CMB) na estação e densidade populacional de perfilhos (DPP). O maior valor encontrado para CMB, foi na estação das águas 210,37 mm versus 175,89 mm para a transição. A DPP apresentou o menor valor (680 perfilhos m <->2) com a maior remoção (2 cm) em relação ao de menor remoção (720 perfilhos m <->2). As diferentes remoções de lâmina foliar proporcionou alteração na altura de saída, em que o tratamento 6 cm apresentou a maior altura (24,31 cm), não diferenciando os demais tratamentos. O índice de área foliar (IAF) e interceptação da radiação fotossinteticamente ativa (IRFA) foram modificados no pós pastejo. A maior remoção de lâmina foliar (2 cm) teve o seu período de descanso (PD) aumentado em 7 dias, em relação a menor remoção (6 cm).(AU)