Dietary supplementation of mineral nanoparticles for channel catfish (Ictalurus punctatus).

This study evaluated the supplementation of iron and copper nanoparticles in channel catfish diets and their influences on growth and health. A comparative feeding trial was carried out for 9 weeks to evaluate combinations of iron and copper nanoparticles: only iron nanoparticles (IronNP), only copper nanoparticles (CopperNP), CopperNP + IronNP, and a control diet supplemented with inorganic iron and copper (FeSO4 and CuSO4). After a 9-week feeding trial, growth performance, hematological parameters, whole-body proximate composition, and intestinal microbiota were evaluated, and fish were subjected to a bacterial challenge against Edwardsiella ictaluri to evaluate the contribution of the experimental treatments to fish health status. No statistical differences were detected for catfish fed the various diets in terms of production performance or survival after bacterial challenge. The hematocrit and RBC counts from fish fed the diet containing copper nanoparticles were significantly lower than the control group. A higher relative abundance of gram-positive bacteria was found in the digesta of catfish fed diets containing copper nanoparticles. Furthermore, in the context of hematology, iron nanoparticles did not impact the blood parameters of channel catfish; however, reduced hematocrits were observed in fish fed the copper nanoparticle diet, which lacked supplemental dietary iron, thus reinforcing the importance of dietary iron to catfish hematopoiesis. Nonetheless, additional studies are needed to investigate the effects of dietary copper nanoparticle supplementation in catfish diets to better illuminate its effects on the intestinal microbiota.

Effect of Parenteral Supplementation of Minerals and Vitamins on Oxidative Stress Biomarkers and Hepatic Fatty Acid Metabolism in Dairy Cows During the Transition Period.

In the present work we aimed to study the effects of parenteral vitamin and mineral supplementation on hepatic fatty acid metabolism as well as on the oxidative stress biomarkers in biological samples of transition cows. The supplemented group (SG, n = 11) received a subcutaneous injection of 5 mL of vitamin A palmitate 35 mg/mL, vitamin E acetate 50 mg/mL plus other injection of 5 mL of copper edetate 10 mg/mL, zinc edetate 40 mg/mL, manganese edetate 10 mg/mL, and sodium selenite 5 mg/mL on days - 60, - 30, and 7 (± 3) relative to calving. The control group (CG, n = 11) received two subcutaneous injections of 5 mL of 9 mg/mL sodium chloride at the same times of the SG. Blood, urine, and liver biopsies were sampled 21 (± 3) days before the expected calving date and 7 and 21 (± 3) days after calving. Results revealed that supplemented animals had higher glutation peroxidase (GSH-Px) activity, lower and higher concentration of 3-nitrotyrosine (3-NT) in the liver and plasma, respectively, higher expression of the mitochondrial beta-oxidation enzyme carnitine palmitoyltransferase 1 in the liver, and lower content of hepatic triacylglycerol, mirroring plasma liver function parameters. No differences between groups were found in the superoxide dismutase activity, MDA concentrations, the protein abundance of peroxisomal acyl-CoA oxidase 1, diacylglycerol O-acyltransferase 1, and peroxisome proliferator-activated receptor alpha. These results suggest that the vitamin and mineral supplementation provided to dairy cows had a beneficial effect on GSH-Px activity, hepatic 3-NT concentration, and on the metabolic adaptation during the peripartum period.

Influence of Molybdenum and Organic Sources of Copper and Sulfur on the Performance, Carcass Traits, Blood Mineral Concentration, and Ceruloplasmin Activity in Lambs.

This study aimed to evaluate the effects of molybdenum (Mo) and organic and inorganic sources of copper (Cu) and sulfur (S) on the performance, carcass traits, and blood concentration of these minerals in lambs. Forty male non-castrated crossbred Dorper x Santa Inês lambs (20 ± 1.2 kg of body weight and 90 ± 2 d of age) were randomly allocated into one of the ten following treatments: (T0) control, basal diet; (T1) Mo; (T2) inorganic Cu and inorganic S; (T3) inorganic Cu and organic S; (T4) organic Cu and inorganic S; (T5) organic Cu and organic S; (T6) Mo plus inorganic Cu and inorganic S; (T7) Mo plus inorganic Cu and organic S; (T8) Mo plus organic Cu and inorganic S; and (T9) Mo plus organic Cu and organic S. Regardless of the source, Mo, Cu, and S were added at levels of 10 mg, 10 mg, and 2000mg/kg DM, respectively. The mineral supplements (Mo, Cu, and S) were added into the total mixed ration (TMR) by mixing them apart with the mineral and vitamin premix and then put into the TMR. The animals were kept in individual pens and received a total mixed ration for 84 days. Body weight and blood sampling was performed every 28 days. All animals were slaughtered after 84 days, and carcass traits were evaluated. Although organic sources of Cu and S added to Mo supplementation had increased the ADG throughout the study, this effect did not reflect in the heavier final BW outcomes for this treatment. In addition, no effect of these treatments was observed on the carcass traits. The serum Cu concentration was higher for the T0 group compared to the other groups; otherwise, Mo reduced the serum Cu concentration compared to the other groups. Considering the interaction among the minerals and their sources at 84 d of study, organic sources of Cu and S treatment and Mo associated with inorganic sources of Cu plus organic S treatment had an increased serum Cu concentration compared to other groups. Regardless of time, organic sources of Cu and S increased serum S concentration. At 84 days after enrollment, serum Mo concentration was lower for the control group compared to the other groups. Further, Mo supplementation increased its blood concentration compared to the control group throughout the study. The control group had the highest ceruloplasmin activity compared to the other groups; otherwise, at 84 d of the study, either Mo or inorganic S supplementation reduced ceruloplasmin activity. Serum ceruloplasmin activity was higher when Cu supplementation, regardless of source, was associated with organic S. However, at d 84 of the study, inorganic Cu associated to organic S supplements increased serum ceruloplasmin activity. In this current study, it was not possible to identify a pattern in the variables studied, however, further studies are needed to confirm that organic sources of Cu and S interacted alone without a defined pattern.

Experiences and Lessons Learned in Developing and Implementing a Population-Based Nutrition and Health Surveillance System in Guatemala 2011-2021.

Background: Practice-based experiences documenting development and implementation of nutrition and health surveillance systems are needed. Objectives: To describe processes, methods, and lessons learned from developing and implementing a population-based household nutrition and health surveillance system in Guatemala. Methods: The phases and methods for the design and implementation of the surveillance system are described. Efforts to institutionalize the system in government institutions are described, and illustrative examples describing different data uses, and lessons learned are provided. Results: After initial assessments of data needs and consultations with officials in government institutions and partners in the country, a population-based nutrition surveillance system prototype with complex sampling was designed and tested in 5 Guatemalan Highland departments in 2011. After dissemination of the prototype, government and partners expanded the content, and multitopic nutrition and health surveillance cycles were collected in 2013, 2015, 2016, 2017/18, and 2018/19 providing nationally representative data for households, women of reproductive age (15-49 y), and children aged 0-59 mo. For each cycle, data were to be collected from 100 clusters, 30 households in each, and 1 woman and 1 child per household. Content covered ∼25 health and nutrition topics, including coverage of all large-scale nutrition-specific interventions; the micronutrient content of fortifiable sugar, salt, and bread samples; anthropometry; and biomarkers to assess annually, or at least once, ∼25 indicators of micronutrient status and chronic disease. Data were collected by 3-5 highly trained field teams. The design was flexible and revised each cycle allowing potential changes to questionnaires, population groups, biomarkers, survey design, or other changes. Data were used to change national guidelines for vitamin A and B-12 interventions, among others, and evaluate interventions. Barriers included frequent changes of high-level government officials and heavy dependence on US funding. Conclusions: This system provides high-quality data, fills critical data gaps, and can serve as a useful model for others.

Effects of chromium yeast supplementation on productive and metabolic responses of laying hens fed diets containing different energy levels

This study evaluated the effects of chromium yeast (Cr yeast) and two apparent metabolizable energy (AME) levels on productive performance, egg quality, and lipid and carbohydrate metabolism in laying hens. A total of 192 Bovans White laying hens at 47 weeks of age were randomly assigned to eight dietary treatments (six replicates each) in a 4 × 2 factorial arrangement: four levels of supplemental Cr (0, 0.2, 0.4, and 0.8 ppm as Cr yeast) and two AME levels (2,780 and 2,900 kcal AME kg−1). No significant effect of Cr yeast supplementation on feed intake, egg weight, egg production, intact eggs, egg mass, feed conversion ratio, or egg quality was observed. Egg quality parameters and Cr content in the yolk were not affected by dietary treatments. Plasma glucose and lipid levels were not influenced by either Cr yeast or AME levels used in this study. However, Cr yeast supplementation improved yolk percentage and hepatic glycogen content. The inclusion of Cr yeast at 0.2 and 0.4 ppm induced the highest hepatic glycogen content with the energy levels 2,900 and 2,780 kcal AME kg−1, respectively. Laying hens fed 2,900 kcal AME kg−1 showed the highest abdominal fat. The results observed in the present study support the hypothesis that the lack of positive effects of Cr yeast supplementation on production performance and egg quality may be related to good management practices, as the birds were not subjected to stressful conditions.

Chronic copper poisoning in beef cattle in the state of Mato Grosso, Brazil / Intoxicação crônica por cobre em bovinos de corte no Estado de Mato Grosso, Brasil

Copper is an essential micromineral in animal feed; however, when consumed in excess, it can cause liver necrosis, hemolytic crisis, hemoglobinuric nephrosis and death in cattle. Although uncommon in this species, copper poisoning occurs as a result of exacerbated supplementation, deficiency of antagonist microminerals, or previous liver lesions. An outbreak of chronic copper poisoning is reported in semi-confined cattle after supplementation with 50 mg/Kg of dry matter copper. The cattle showed clinical signs characterized by anorexia, motor incoordination, loss of balance, jaundice, brownish or black urine, diarrhea and death, or were found dead, 10 to 302 days after consumption. Of the 35 cattle that died, 20 underwent necropsy, whose frequent findings were jaundice, enlarged liver with evident lobular pattern, black kidneys, and urinary bladder with brownish to blackish content. Microscopically, the liver showed vacuolar degeneration and/or zonal hepatocellular centrilobular or paracentral coagulative necrosis, in addition to cholestasis, mild periacinal fibrosis, apoptotic bodies, and mild to moderate mononuclear inflammation. Degeneration and necrosis of the tubular epithelium and intratubular hemoglobin cylinders were observed in the kidneys. Copper levels in the liver and kidneys ranged from 5,901.24 to 28,373.14 µmol/kg and from 303.72 to 14,021 µmol/kg, respectively. In conclusion, copper poisoning due to excessive nutritional supplementation is an important cause of jaundice, hemoglobinuria, and death in semi-confined cattle.(AU)

Cobre é um micromineral essencial, que quando em excesso induz necrose hepática, crise hemolítica, nefrose hemoglobínurica e morte em bovinos. As intoxicações, apesar de incomuns nessa espécie, ocorrem devido a suplementação exacerbada de cobre, pela deficiência de microminerais antagonistas ou secundária a lesão hepática prévia. Relata-se um surto de intoxicação crônica por cobre em bovinos semiconfinados após suplementação com 50mg/kg de cobre em matéria seca. Os bovinos manifestaram sinais clínicos caracterizados por anorexia, incoordenação motora, perda de equilíbrio, icterícia, urina acastanhada ou negra, diarreia e morte ou foram encontrados mortos, após 10 a 302 dias do início de consumo. De 35 bovinos que morreram 20 foram submetidos à necropsia sendo achada frequente icterícia, fígado aumentado e com padrão lobular evidente, rins pretos e bexiga urinária repleta de conteúdo acastanhado a enegrecida. Microscopicamente, no fígado havia degeneração vacuolar e ou necrose coagulativa hepatocelular zonal, centrolobular ou paracentral, além de degeneração vacuolar com corpúsculos de Councilman, colestase, fibrose periascinar leve, e inflamação de discreta a moderada. Nos rins havia degeneração e necrose do epitélio tubular assim como cilindros de hemoglobina intratubulares. Os níveis de cobre no fígado e rim foram de 5.901,24 a 28.373,14µmol/kg e 303,72 a 14.021µmol/kg respectivamente. A suplementação nutricional excessiva com cobre pode causar doença hemolítica com icterícia, hemoglobinúria e morte de bovinos mantidos em sistema de semiconfinamento.(AU)

Chronic copper poisoning in beef cattle in the state of Mato Grosso, Brazil / Intoxicação crônica por cobre em bovinos de corte no Estado de Mato Grosso, Brasil

Copper is an essential micromineral in animal feed; however, when consumed in excess, it can cause liver necrosis, hemolytic crisis, hemoglobinuric nephrosis and death in cattle. Although uncommon in this species, copper poisoning occurs as a result of exacerbated supplementation, deficiency of antagonist microminerals, or previous liver lesions. An outbreak of chronic copper poisoning is reported in semi-confined cattle after supplementation with 50 mg/Kg of dry matter copper. The cattle showed clinical signs characterized by anorexia, motor incoordination, loss of balance, jaundice, brownish or black urine, diarrhea and death, or were found dead, 10 to 302 days after consumption. Of the 35 cattle that died, 20 underwent necropsy, whose frequent findings were jaundice, enlarged liver with evident lobular pattern, black kidneys, and urinary bladder with brownish to blackish content. Microscopically, the liver showed vacuolar degeneration and/or zonal hepatocellular centrilobular or paracentral coagulative necrosis, in addition to cholestasis, mild periacinal fibrosis, apoptotic bodies, and mild to moderate mononuclear inflammation. Degeneration and necrosis of the tubular epithelium and intratubular hemoglobin cylinders were observed in the kidneys. Copper levels in the liver and kidneys ranged from 5,901.24 to 28,373.14 µmol/kg and from 303.72 to 14,021 µmol/kg, respectively. In conclusion, copper poisoning due to excessive nutritional supplementation is an important cause of jaundice, hemoglobinuria, and death in semi-confined cattle.(AU)

Cobre é um micromineral essencial, que quando em excesso induz necrose hepática, crise hemolítica, nefrose hemoglobínurica e morte em bovinos. As intoxicações, apesar de incomuns nessa espécie, ocorrem devido a suplementação exacerbada de cobre, pela deficiência de microminerais antagonistas ou secundária a lesão hepática prévia. Relata-se um surto de intoxicação crônica por cobre em bovinos semiconfinados após suplementação com 50mg/kg de cobre em matéria seca. Os bovinos manifestaram sinais clínicos caracterizados por anorexia, incoordenação motora, perda de equilíbrio, icterícia, urina acastanhada ou negra, diarreia e morte ou foram encontrados mortos, após 10 a 302 dias do início de consumo. De 35 bovinos que morreram 20 foram submetidos à necropsia sendo achada frequente icterícia, fígado aumentado e com padrão lobular evidente, rins pretos e bexiga urinária repleta de conteúdo acastanhado a enegrecida. Microscopicamente, no fígado havia degeneração vacuolar e ou necrose coagulativa hepatocelular zonal, centrolobular ou paracentral, além de degeneração vacuolar com corpúsculos de Councilman, colestase, fibrose periascinar leve, e inflamação de discreta a moderada. Nos rins havia degeneração e necrose do epitélio tubular assim como cilindros de hemoglobina intratubulares. Os níveis de cobre no fígado e rim foram de 5.901,24 a 28.373,14µmol/kg e 303,72 a 14.021µmol/kg respectivamente. A suplementação nutricional excessiva com cobre pode causar doença hemolítica com icterícia, hemoglobinúria e morte de bovinos mantidos em sistema de semiconfinamento.(AU)

Influence of organic chromium supplementation on the performance of beef calves undergoing weaning-related stress / Influência da suplementação com cromo orgânico no desempenho de bezerros de corte submetidos ao estresse da desmama

Dietary chromium supplementation before, during, and after weaning was conducted to evaluate the hypothesis that chromium supplementation could reduce weaning-induced cortisol release in beef calves. We examined the effects of chromium supplementation in 150 crossbred calves (male and female) between five and six months of age. The calves were randomly divided by sex and breed into two equal homogeneous groups (n=75). One group was used as the control, and the other experimental group received supplementation with 0.9mg of chromium carbon-amino-phospho-chelate per 100kg BW. The chromium supplement was mixed with mineral salt for the consumption of 0.1% of BW, and the supplement was administered via creep feeding 60 days before and 60 days after forced weaning. Calves were weighed, and their blood and urine samples were obtained at four time-points: T0 (60 days before weaning), T1 (at weaning), T2 (48 hours after weaning), and T3 (60 days after weaning). Blood samples were used to determine chromium, cortisol, total protein, and albumin concentrations, and urine samples were used to determine urinary creatinine and chromium levels. Cumulative weight gain was higher in calves supplemented with chromium before weaning and during the experiment (P<0.05). In addition, weaning-related stress caused an increase in chromium excretion in the urine, and chromium supplementation reduced stress, which resulted in lower cortisol and total protein levels during weaning.(AU)

O estudo foi realizado para avaliar a hipótese de que a suplementação dietética com cromo antes, durante e após a desmama possa diminuir a concentração de cortisol causado por este processo em bezerros de corte. Para tal, foram utilizados 150 bezerros mestiços, machos e fêmeas, entre cinco e seis meses de idade. Os animais foram divididos randomicamente por sexo e grupo genético em dois grupos homogêneos (n=75), um mantido como controle e outro suplementado com 0,9mg de carboaminofosfoquelato de cromo/100 kg PV misturado a um sal proteinado para ser consumido na base de 0,1% do PV via creep feeding, no decorrer de 60 dias antes e 60 dias após à desmama forçada. Os animais foram pesados e foram coletadas amostras sanguíneas e urinárias no M0 (60 dias antes da desmama), M1 (desmama), M2 (48 horas após a desmama) e M3 (60 dias após à desmama) para determinação de cromo, cortisol, proteína total e albumina no sangue e da concentração urinária de creatinina e cromo. O ganho acumulado de peso foi superior nos bezerros suplementados com cromo antes da desmama e no decorrer de todo o experimento (P<0,05). A suplementação com cromo reduziu os teores de cortisol e de proteína total durante a desmama. O estresse da desmama provocou aumento da excreção de cromo pela urina.(AU)

Influence of organic chromium supplementation on the performance of beef calves undergoing weaning-related stress / Influência da suplementação com cromo orgânico no desempenho de bezerros de corte submetidos ao estresse da desmama

Dietary chromium supplementation before, during, and after weaning was conducted to evaluate the hypothesis that chromium supplementation could reduce weaning-induced cortisol release in beef calves. We examined the effects of chromium supplementation in 150 crossbred calves (male and female) between five and six months of age. The calves were randomly divided by sex and breed into two equal homogeneous groups (n=75). One group was used as the control, and the other experimental group received supplementation with 0.9mg of chromium carbon-amino-phospho-chelate per 100kg BW. The chromium supplement was mixed with mineral salt for the consumption of 0.1% of BW, and the supplement was administered via creep feeding 60 days before and 60 days after forced weaning. Calves were weighed, and their blood and urine samples were obtained at four time-points: T0 (60 days before weaning), T1 (at weaning), T2 (48 hours after weaning), and T3 (60 days after weaning). Blood samples were used to determine chromium, cortisol, total protein, and albumin concentrations, and urine samples were used to determine urinary creatinine and chromium levels. Cumulative weight gain was higher in calves supplemented with chromium before weaning and during the experiment (P<0.05). In addition, weaning-related stress caused an increase in chromium excretion in the urine, and chromium supplementation reduced stress, which resulted in lower cortisol and total protein levels during weaning.(AU)

O estudo foi realizado para avaliar a hipótese de que a suplementação dietética com cromo antes, durante e após a desmama possa diminuir a concentração de cortisol causado por este processo em bezerros de corte. Para tal, foram utilizados 150 bezerros mestiços, machos e fêmeas, entre cinco e seis meses de idade. Os animais foram divididos randomicamente por sexo e grupo genético em dois grupos homogêneos (n=75), um mantido como controle e outro suplementado com 0,9mg de carboaminofosfoquelato de cromo/100 kg PV misturado a um sal proteinado para ser consumido na base de 0,1% do PV via creep feeding, no decorrer de 60 dias antes e 60 dias após à desmama forçada. Os animais foram pesados e foram coletadas amostras sanguíneas e urinárias no M0 (60 dias antes da desmama), M1 (desmama), M2 (48 horas após a desmama) e M3 (60 dias após à desmama) para determinação de cromo, cortisol, proteína total e albumina no sangue e da concentração urinária de creatinina e cromo. O ganho acumulado de peso foi superior nos bezerros suplementados com cromo antes da desmama e no decorrer de todo o experimento (P<0,05). A suplementação com cromo reduziu os teores de cortisol e de proteína total durante a desmama. O estresse da desmama provocou aumento da excreção de cromo pela urina.(AU)

Supplementation with an Inorganic Iron Source Modulates the Metalloproteomic Profile of the Royal Jelly Produced by Apis mellifera L.

This study aimed to evaluate the quality of the royal jelly produced by Apis mellifera bees in the presence of different iron concentrations (ferrous sulfate heptahydrate-0, 25, 50, and 100 mg L-1). Two-dimensional electrophoresis was used for the fractionation of royal jelly proteins, and iron level was quantified using flame atomic absorption spectrometry technique. The proteins were identified using electrospray ionisation mass spectrometry. Analysis of variance followed by the Tukey test (P < 0.05) was utilised. Dietary supplementation with mineral Fe affected the protein content and number of proteins in the experimental period. Further, the diet containing the highest iron concentration showed a greater number of spots containing iron, as well as in the abdomen of the bees. The most protein containing Fe were classified as major royal jelly proteins. These results showed that Fe influenced the quality of royal jelly and can improve its nutritional value.

Nutritional plans of digestible phosphorus for gilts from 30 to 100 kg

The objective of this study was to evaluate sequential digestible phosphorus levels for gilts from 30 to 100 kg. Sixty gilts were used, with an initial weight of 29.98±3.37 kg and final weight of 100.66±8.82 kg, distributed in a randomized block design, with five nutritional digestible phosphorus levels: 2.19-1.88-1.68, 2.69-2.38-2.18, 3.19-2.88-2.68, 3.69-3.38-3.18, and 4.19-3.88-3.68 g kg−1, respectively, for the growth phases 30 to 50 kg, 50 to 70 kg, and 70 to 100 kg, with six replicates and two animals per pen. The nutritional plans did not influence the final weight, days to reach 100 kg, total and daily weight gain, total and daily feed intake, crude protein intake, digestible lysine intake, or metabolizable energy intake. An increase in digestible phosphorus and calcium intake was observed according to the increase in the digestible phosphorus level in the diet. There was a significant effect on feed conversion. No effect of nutritional plans was observed for backfat thickness, muscle depth, loin eye area, lean meat percentage, or carcass bonus index. There was no difference in the production of dry and natural matter or the residue coefficient. The effect of the nutritional digestible phosphorus levels in the contents of total solids and volatile solids was verified. There was a difference in the concentration of total nitrogen and total phosphorus in the manure. The nutritional plan with 3.19-2.88-2.68 and 3.69-3.38-3.18 g kg−1 of digestible phosphorus results in better feed conversion than the basal diet. For a lower excretion of phosphorus in the manure, the recommended nutritional plan is 2.19-1.88-1.68 g kg−1 of digestible phosphorus.(AU)

Bio-economic evaluation of a reduced phosphorus supplementation strategy for a cow-calf system in Brazil: a case study.

Fertility, weight of calves at weaning, and the economic aspects of a breeding herd receiving mineral supplements containing 75 or 12.5 g of phosphorus (P)/kg were measured from 2013 to 2016. No differences in reproduction parameters or weight at weaning were found before and after the adoption of the new scheme of mineral supplementation. Before the study, the annual cost with the formula containing more P was equivalent to 29.3 weaned beef calves; after the P reduction, the annual cost was equivalent to 2.2 to 6.8 weaned calves. After 3 years of supplementation with 12.5 g P/kg no signs of P deficiency were observed. The clinical-nutritional diagnosis of the herd indicated no cause-effect of P content of mineral supplements upon fertility or performance of healthy cows, demonstranting that the adequate forage allowance was enough to meet most P required by the cows.

Mineralization in newborn calves contributes to health, improve the antioxidant system and reduces bacterial infections.

The first phase of life of dairy calves has elevated mortality indices linked with low immunity and sanitary challenges, mainly bacterial infections are involved in the pathogenesis of diarrhea, the leading cause of death. Also, other important problem is the nutritional deficiencies, such as the mineral deficiency. Thus, the aim of this study was to evaluate whether an intramuscular mineral supplementation based on selenium, copper, potassium, magnesium and phosphorus possess beneficial effects on health of dairy calves. For this, ten calves were divided in two groups: the group A was supplemented with injectable mineral, while the group B was used as control group (without mineral supplementation). The mineral complex was administrated via intramuscularly at dose of 3 mL/animal on days 2 and 14 post-birth. The total blood was collected on days 2, 10, 20 and 30 of life of animals in order to analyze the antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)), blood count and seric biochemistry linked with proteic, lipid and carbohydrate metabolism. Feces samples were also collected on days 10, 20 and 30 of life of animals to perform the total bacterial count, parasitological exam and fecal consistency score. Moreover, the weight and corporal temperature were also evaluated. The mineral supplementation presented beneficial properties to calves from birth to the 30th of life through the increase on activity of antioxidant enzymes, improvement of immunity, and avoiding problems linked with diarrhea and anemia, can be considered an interesting approach to prevent these alterations linked with high mortality in the period of life.

Effect Of Ad Libitum Supplementation Selenium And Selenium Serum In Nellore Cattle / Efeito Da Suplementação Ad Libitum Com Selênio E O Selênio Sérico Em Bovinos Nelore / Efecto De Suplementación Ad Libitum Con Seleccion Y El Selenio Sérico En Bovinos Nelore

Selenium (Se) is an essential micromineral for cattle but the researches in Brazil with mineral elements are scarce. An optimal dosage of Se should be determined for the different cattle breeds although this is not well known for Nelore cattle. It was aimed to evaluate the effects of different levels of Se supplementation on serum Se concentration in Nelore cattle for 150 days. It was used 60 male, uncastrated Nellore calves (~ 12 mo) grazing on Brachiaria decumbens pasture, which was Se deficient (0.04 mg Se/kg dry matter) and fed on mineral mixture ad libitum supplemented by Se at daily concentrations of 0 mg (Gc; N= 15), 3.6 mg (G3.6; N= 15), 5.4 mg (G5.4; N= 15), or 6.4 mg (G6.4; N= 15). In Gc Se concentration decreased significantly (P<0.01) from day 0 (105.68 ± 51.87 g/L) on 120 (81.08 ± 40.92 g/L) and the cattle had marginal Se deficiency. In G3.6, Se levels increased progressively (P<0.01) throughout the experiment and reached normal values by the end (103.09 ± 47.29 g/L). In G5.4 Se concentrations were higher (P<0.01) on day 120 (95.37 ± 37.89 g/L) than on day 0 (59.02 ± 24.29 g/L), whereas in G6.4 they were high (P<0,01) on day 60 (87.78 ± 40.87 g/L) of supplementation, but animals from both groups had marginal Se deficiency. It was concluded that the supplementation dosage of 3.6 mg Se/animal/day is efficient to prevent Se deficiency in Nelore cattle.(AU)

O selênio (Se) é um micromineral essencial para os bovinos, mas no Brasil as pesquisas com esse elemento mineral são escassas e, ainda não está totalmente determinado uma dose ideal para suplementar os bovinos da raça Nelore. Objetivou-se avaliar os efeitos de diferentes níveis de suplementação com Se sobre o Se sérico em bovinos da raça Nelore por 150 dias. Foi utilizado 60 bezerros, machos não castrados da raça Nelore (~ 12 meses) mantidos em pastagem de Brachiaria decumbens, que era deficiente em Se (0,04 mg de Se/kg MS) e suplementos ad libitum com mistura mineral que consumiram doses diárias de 0 mg (Gc; N= 15), 3,6 mg (G3,6; N= 15), 5,4 mg (G5,4; N= 15) ou 6,4 mg (G6,4; N= 15). No Gc a concentração de Se diminuiu significativamente (P<0,01) do dia 0 (105,68 ± 51,87 g/L) ao 120 (81,08 ± 40,92 g/L) e os animais apresentaram deficiência marginal no Se sérico. No G3.6, Se sérico aumentou progressivamente (P<0,01) durante o experimento, atingindo valores normais no dia 120 (103,09 ± 47,29 g/L). No G5.4, concentração de Se foi superior (P<0,01) no dia 120 (95,37 ± 37,89 g/L), enquanto que em G6.4 elevou (P<0,01) no dia 60 (87,78 ± 40,87 g/L), mas em ambos grupos as concentrações séricas de Se eram consideradas deficiência marginal. Conclui-se que a suplementação com 3,6 mg Se/animal/dia, é eficaz para prevenir a deficiência de Se em bovinos Nelore.(AU)

El selenio (Se) es un micromineral esencial para los bovinos, pero en Brasil las investigaciones con ese elemento mineral son escasas y, aún no está totalmente determinado una dosis ideal para suplementar los bovinos de la raza Nelore. Se evaluó los efectos de diferentes niveles de suplementación de Se sobre el Se sérico en bovinos de la raza Nelore por 150 días. Se utilizaron 60 terneros, machos no castrados de la raza nelore (~ 12 meses) mantenidos en pastoreo de Brachiaria decumbens, que era deficiente en Se (0,04 mg de Se/Kg MS) y suplementos ad libitum con mezcla mineral que consumieron dosis diarias 0 mg (Gc, N= 15), 3,6 mg (G3,6; N= 15), 5,4 mg (G5,4; N= 15) o 6,4 mg (G6,4; N= 15). En el Gc la concentración de Se disminuyó significativamente (P<0,01) del día 0 (105,68 ± 51,87 g/L) al 120 (81,08 ± 40,92 g/L) y los animales presentaron deficiencia marginal en el sérico. En el G3.6, si el sérico aumentó progresivamente (P<0,01) durante el experimento, alcanzando valores normales en el día 120 (103,09 ± 47,29 g/L). En el G5,4, concentración de Se fue superior (P<0,01) el día 120 (95,37 ± 37,89 g/L), mientras que en G6,4 elevó (P<0,01) el día 60 (87,78 ± 40,87 g/L), pero en ambos grupos las concentraciones séricas de Si se consideraban deficiencia marginal. Se concluye que la suplementación con 3,6 mg Se/animal/día, es eficaz para prevenir la deficiencia de Se en bovinos.(AU)

Effect Of Ad Libitum Supplementation Selenium And Selenium Serum In Nellore Cattle / Efeito Da Suplementação Ad Libitum Com Selênio E O Selênio Sérico Em Bovinos Nelore / Efecto De Suplementación Ad Libitum Con Seleccion Y El Selenio Sérico En Bovinos Nelore

Selenium (Se) is an essential micromineral for cattle but the researches in Brazil with mineral elements are scarce. An optimal dosage of Se should be determined for the different cattle breeds although this is not well known for Nelore cattle. It was aimed to evaluate the effects of different levels of Se supplementation on serum Se concentration in Nelore cattle for 150 days. It was used 60 male, uncastrated Nellore calves (~ 12 mo) grazing on Brachiaria decumbens pasture, which was Se deficient (0.04 mg Se/kg dry matter) and fed on mineral mixture ad libitum supplemented by Se at daily concentrations of 0 mg (Gc; N= 15), 3.6 mg (G3.6; N= 15), 5.4 mg (G5.4; N= 15), or 6.4 mg (G6.4; N= 15). In Gc Se concentration decreased significantly (P<0.01) from day 0 (105.68 ± 51.87 g/L) on 120 (81.08 ± 40.92 g/L) and the cattle had marginal Se deficiency. In G3.6, Se levels increased progressively (P<0.01) throughout the experiment and reached normal values by the end (103.09 ± 47.29 g/L). In G5.4 Se concentrations were higher (P<0.01) on day 120 (95.37 ± 37.89 g/L) than on day 0 (59.02 ± 24.29 g/L), whereas in G6.4 they were high (P<0,01) on day 60 (87.78 ± 40.87 g/L) of supplementation, but animals from both groups had marginal Se deficiency. It was concluded that the supplementation dosage of 3.6 mg Se/animal/day is efficient to prevent Se deficiency in Nelore cattle.

O selênio (Se) é um micromineral essencial para os bovinos, mas no Brasil as pesquisas com esse elemento mineral são escassas e, ainda não está totalmente determinado uma dose ideal para suplementar os bovinos da raça Nelore. Objetivou-se avaliar os efeitos de diferentes níveis de suplementação com Se sobre o Se sérico em bovinos da raça Nelore por 150 dias. Foi utilizado 60 bezerros, machos não castrados da raça Nelore (~ 12 meses) mantidos em pastagem de Brachiaria decumbens, que era deficiente em Se (0,04 mg de Se/kg MS) e suplementos ad libitum com mistura mineral que consumiram doses diárias de 0 mg (Gc; N= 15), 3,6 mg (G3,6; N= 15), 5,4 mg (G5,4; N= 15) ou 6,4 mg (G6,4; N= 15). No Gc a concentração de Se diminuiu significativamente (P<0,01) do dia 0 (105,68 ± 51,87 g/L) ao 120 (81,08 ± 40,92 g/L) e os animais apresentaram deficiência marginal no Se sérico. No G3.6, Se sérico aumentou progressivamente (P<0,01) durante o experimento, atingindo valores normais no dia 120 (103,09 ± 47,29 g/L). No G5.4, concentração de Se foi superior (P<0,01) no dia 120 (95,37 ± 37,89 g/L), enquanto que em G6.4 elevou (P<0,01) no dia 60 (87,78 ± 40,87 g/L), mas em ambos grupos as concentrações séricas de Se eram consideradas deficiência marginal. Conclui-se que a suplementação com 3,6 mg Se/animal/dia, é eficaz para prevenir a deficiência de Se em bovinos Nelore.

El selenio (Se) es un micromineral esencial para los bovinos, pero en Brasil las investigaciones con ese elemento mineral son escasas y, aún no está totalmente determinado una dosis ideal para suplementar los bovinos de la raza Nelore. Se evaluó los efectos de diferentes niveles de suplementación de Se sobre el Se sérico en bovinos de la raza Nelore por 150 días. Se utilizaron 60 terneros, machos no castrados de la raza nelore (~ 12 meses) mantenidos en pastoreo de Brachiaria decumbens, que era deficiente en Se (0,04 mg de Se/Kg MS) y suplementos ad libitum con mezcla mineral que consumieron dosis diarias 0 mg (Gc, N= 15), 3,6 mg (G3,6; N= 15), 5,4 mg (G5,4; N= 15) o 6,4 mg (G6,4; N= 15). En el Gc la concentración de Se disminuyó significativamente (P<0,01) del día 0 (105,68 ± 51,87 g/L) al 120 (81,08 ± 40,92 g/L) y los animales presentaron deficiencia marginal en el sérico. En el G3.6, si el sérico aumentó progresivamente (P<0,01) durante el experimento, alcanzando valores normales en el día 120 (103,09 ± 47,29 g/L). En el G5,4, concentración de Se fue superior (P<0,01) el día 120 (95,37 ± 37,89 g/L), mientras que en G6,4 elevó (P<0,01) el día 60 (87,78 ± 40,87 g/L), pero en ambos grupos las concentraciones séricas de Si se consideraban deficiencia marginal. Se concluye que la suplementación con 3,6 mg Se/animal/día, es eficaz para prevenir la deficiencia de Se en bovinos.

Chelated minerals and two limestone particle sizes on production of layers in the second laying cycle / Minerais quelatados e duas granulometrias de calcário sobre a produção de poedeiras em segundo ciclo produtivo

The aim of this experiment was to evaluate the performance, egg quality, and bone quality of commercial white-egg layer hens in the second production cycle fed diets containing organic and inorganic minerals and two limestone particle sizes. A total of 215 birds with an average weight of 1.527 ± 0.092 kg were distributed in a completely randomized design in a 2 × 2 factorial arrangement (two mineral sources and two limestone particle sizes) with six replicates. The following four treatments were tested: T1 = inorganic minerals + 100% fine limestone; T2 = inorganic minerals + 50% fine limestone + 50% coarse limestone; T3 = organic minerals + 100% fine limestone; and T4 = organic minerals+ 50% fine limestone + 50% coarse limestone. No significant interaction occurred between the studied factors for any variable. Egg production, egg mass, bone resistance and bone deformity were higher with organic supplementation. Eggshell weight, percentage of eggshell, and specific gravity were higher in the treatments with the fine+coarse limestone association. In conclusion, organic mineral supplementation improves the performance based on egg production and egg mass. The association between fine and larger-sized limestone improves the external quality of eggs. The use of organic minerals improves the bone quality of birds in the second production cycle.

Neste experimento objetivou-se de avaliar o desempenho, qualidade dos ovos e qualidade óssea das poedeiras comerciais leves em 2° ciclo de produção, submetidas às dietas contendo minerais orgânicos e inorgânicos e duas granulometrias do calcário. Foram utilizadas 216 aves, com peso médio de 1,527 ± 0,092 kg, distribuídas em um delineamento inteiramente casualizado em um esquema fatorial 2 × 2 (duas fontes de minerais e duas granulometrias do calcário), com seis repetições. Foram empregados quatro tratamentos a seguir: T1 = minerais inorgânicos + 100% de calcário fino; T2 = minerais inorgânicos + 50% de calcário fino + 50% de calcário grosso; T3 = minerais orgânicos + 100% de calcário fino e T4 = minerais orgânicos + 50% de calcário fino + 50 % de calcário grosso. Não houve interação significativa entre os fatores estudados para nenhuma variável. A produção de ovos, massa de ovo, resistência e deformidade óssea foram melhores com a suplementação orgânica. O peso da casca, percentagem da casca e a gravidade específica foram melhores nos tratamentos com a associação do calcário fino + grosso. Concluiu-se que suplementação mineral orgânica melhora o desempenho com base na produção de ovos e massa dos ovos. A utilização da associação do calcário fino com o de maior granulometria melhora a qualidade externa dos ovos. A utilização de minerais orgânicos melhora a qualidade óssea das aves, em 2° ciclo produtivo.

Chelated minerals and two limestone particle sizes on production of layers in the second laying cycle / Minerais quelatados e duas granulometrias de calcário sobre a produção de poedeiras em segundo ciclo produtivo

The aim of this experiment was to evaluate the performance, egg quality, and bone quality of commercial white-egg layer hens in the second production cycle fed diets containing organic and inorganic minerals and two limestone particle sizes. A total of 215 birds with an average weight of 1.527 ± 0.092 kg were distributed in a completely randomized design in a 2 × 2 factorial arrangement (two mineral sources and two limestone particle sizes) with six replicates. The following four treatments were tested: T1 = inorganic minerals + 100% fine limestone; T2 = inorganic minerals + 50% fine limestone + 50% coarse limestone; T3 = organic minerals + 100% fine limestone; and T4 = organic minerals+ 50% fine limestone + 50% coarse limestone. No significant interaction occurred between the studied factors for any variable. Egg production, egg mass, bone resistance and bone deformity were higher with organic supplementation. Eggshell weight, percentage of eggshell, and specific gravity were higher in the treatments with the fine+coarse limestone association. In conclusion, organic mineral supplementation improves the performance based on egg production and egg mass. The association between fine and larger-sized limestone improves the external quality of eggs. The use of organic minerals improves the bone quality of birds in the second production cycle.(AU)

Neste experimento objetivou-se de avaliar o desempenho, qualidade dos ovos e qualidade óssea das poedeiras comerciais leves em 2° ciclo de produção, submetidas às dietas contendo minerais orgânicos e inorgânicos e duas granulometrias do calcário. Foram utilizadas 216 aves, com peso médio de 1,527 ± 0,092 kg, distribuídas em um delineamento inteiramente casualizado em um esquema fatorial 2 × 2 (duas fontes de minerais e duas granulometrias do calcário), com seis repetições. Foram empregados quatro tratamentos a seguir: T1 = minerais inorgânicos + 100% de calcário fino; T2 = minerais inorgânicos + 50% de calcário fino + 50% de calcário grosso; T3 = minerais orgânicos + 100% de calcário fino e T4 = minerais orgânicos + 50% de calcário fino + 50 % de calcário grosso. Não houve interação significativa entre os fatores estudados para nenhuma variável. A produção de ovos, massa de ovo, resistência e deformidade óssea foram melhores com a suplementação orgânica. O peso da casca, percentagem da casca e a gravidade específica foram melhores nos tratamentos com a associação do calcário fino + grosso. Concluiu-se que suplementação mineral orgânica melhora o desempenho com base na produção de ovos e massa dos ovos. A utilização da associação do calcário fino com o de maior granulometria melhora a qualidade externa dos ovos. A utilização de minerais orgânicos melhora a qualidade óssea das aves, em 2° ciclo produtivo.(AU)

Avaliação econômica de três tipos de suplementação mineral para bovinos de corte no Estado do Pará / Economic evaluation of three strategies of mineral supplementation for beef cattle in State of Pará, Brazil

Em uma propriedade localizada na região nordeste do Estado do Pará foram analisadas alternativas de suplementação mineral para bovinos de corte, da raça nelore, machos, com idades entre 18 e 20 meses e pesos de 299,5 a 308,5 kg. Foram avaliados o custo benefício, os valores dos minerais no fígado e nas costelas e o ganho de peso médio diário dos animais submetidos aos diferentes tratamentos. O estudo foi realizado durante seis meses (março a agosto de 2012), no período de ótima oferta de pastagem. Foram utilizados 60 bovinos, divididos em três grupos de 20 animais em delineamento experimental inteiramente casualizado. O Grupo 1 foi suplementado com uma mistura mineral seletiva (MMS) composta por 25kg de NaCl, 50kg de fosfato bicálcico,190g de sulfato de Cobre, 60g de sulfato de Cobalto e 15g de selenito de sódio; essa mistura continha, em um quilograma, 133g de Na, 123g de P, 631 mg de Cu, 167mg de Co e 9 mg de Se; essa mistura foi proposta com base no prévio diagnóstico clínico-nutricional do rebanho. O Grupo 2 foi suplementado apenas com NaCl (Grupo controle) e o Grupo 3 recebeu uma mistura mineral comercial (MMC), rotineiramente utilizada na fazenda, contendo, em um quilograma, 130g de P, 220g de Ca, 18g de Mg, 36g de S, 6.000mg de Zn, 1.500mg de Cu, 2.000mg de Mn, 200mg de Co, 90mg de I e 36mg de Se. O melhor custo benefício foi obtido com a mistura mineral seletiva. A pastagem não contém Co suficiente para atender as necessidades dos bovinos do grupo controle, mas foi capaz de suprir as necessidades de P, Cu, Zn e Se durante os cinco meses do experimento.(AU)

Three alternatives of mineral supplementation of 18 to 20-month-old male Nelore cattle with 299.5 to 308.5 kg of body weight were used at a farm in northeast of Pará state, Brazil. To evaluate which mineral mixture resulted in better cost-benefit, three groups of 20 head were supplemented for six months, from March to August of 2012. Group 1 was supplemented with a selected mineral mixture (SMM) composed of 25kg NaCl, 50kg dicalcium phosphate, 190g copper sulphate, 60g cobalt sulphate, and 15g sodium selenite, having 133g Na, 123g P, 631mg Cu, 167mg Co, and 9mg Se per kg; this mixture was made based in previous clinical-nutritional examination of herd. Group 2 (control), supplemented only with NaCl; and group 3 received a commercial mineral mixture (CMM), routinely used in this farm, with 130g P, 220g Ca, 18g Mg, 36g S, 6000mg Zn, 1500mg Cu, 2000mg Mn, 200mg Co, 90mg I, and 36mg Se per kg. Concentrations of phosphorus, copper, zinc and iron were determined in liver and 12th ribs. The best cost-benefit alternative was the SMM; the forage alone did not contain enough Co to meet the requirements of the control group, but provided enough P, Cu, Zn and Se during the five months of the experiment.(AU)

Avaliação econômica de três tipos de suplementação mineral para bovinos de corte no Estado do Pará / Economic evaluation of three strategies of mineral supplementation for beef cattle in State of Pará, Brazil

Em uma propriedade localizada na região nordeste do Estado do Pará foram analisadas alternativas de suplementação mineral para bovinos de corte, da raça nelore, machos, com idades entre 18 e 20 meses e pesos de 299,5 a 308,5 kg. Foram avaliados o custo benefício, os valores dos minerais no fígado e nas costelas e o ganho de peso médio diário dos animais submetidos aos diferentes tratamentos. O estudo foi realizado durante seis meses (março a agosto de 2012), no período de ótima oferta de pastagem. Foram utilizados 60 bovinos, divididos em três grupos de 20 animais em delineamento experimental inteiramente casualizado. O Grupo 1 foi suplementado com uma mistura mineral seletiva (MMS) composta por 25kg de NaCl, 50kg de fosfato bicálcico,190g de sulfato de Cobre, 60g de sulfato de Cobalto e 15g de selenito de sódio; essa mistura continha, em um quilograma, 133g de Na, 123g de P, 631 mg de Cu, 167mg de Co e 9 mg de Se; essa mistura foi proposta com base no prévio diagnóstico clínico-nutricional do rebanho. O Grupo 2 foi suplementado apenas com NaCl (Grupo controle) e o Grupo 3 recebeu uma mistura mineral comercial (MMC), rotineiramente utilizada na fazenda, contendo, em um quilograma, 130g de P, 220g de Ca, 18g de Mg, 36g de S, 6.000mg de Zn, 1.500mg de Cu, 2.000mg de Mn, 200mg de Co, 90mg de I e 36mg de Se. O melhor custo benefício foi obtido com a mistura mineral seletiva. A pastagem não contém Co suficiente para atender as necessidades dos bovinos do grupo controle, mas foi capaz de suprir as necessidades de P, Cu, Zn e Se durante os cinco meses do experimento.(AU)

Three alternatives of mineral supplementation of 18 to 20-month-old male Nelore cattle with 299.5 to 308.5 kg of body weight were used at a farm in northeast of Pará state, Brazil. To evaluate which mineral mixture resulted in better cost-benefit, three groups of 20 head were supplemented for six months, from March to August of 2012. Group 1 was supplemented with a selected mineral mixture (SMM) composed of 25kg NaCl, 50kg dicalcium phosphate, 190g copper sulphate, 60g cobalt sulphate, and 15g sodium selenite, having 133g Na, 123g P, 631mg Cu, 167mg Co, and 9mg Se per kg; this mixture was made based in previous clinical-nutritional examination of herd. Group 2 (control), supplemented only with NaCl; and group 3 received a commercial mineral mixture (CMM), routinely used in this farm, with 130g P, 220g Ca, 18g Mg, 36g S, 6000mg Zn, 1500mg Cu, 2000mg Mn, 200mg Co, 90mg I, and 36mg Se per kg. Concentrations of phosphorus, copper, zinc and iron were determined in liver and 12th ribs. The best cost-benefit alternative was the SMM; the forage alone did not contain enough Co to meet the requirements of the control group, but provided enough P, Cu, Zn and Se during the five months of the experiment.(AU)

Reduction of phosphorus concentration in mineral supplement on fertility rate, maternal ability and costs of beef cows reared in pastures of Urochloa decumbens.

Manufacturing and marketing of mineral mixtures with less than 40 g kg(-1) phosphorus (P) is prohibited under Brazilian regulations, although scientific evidence rejects this recommendation. Considering the hypothesis that P levels in commercial mineral supplements can be reduced without affecting animal performance and health, the objective of this experiment was to evaluate the effects of reducing the concentration of P in the mineral supplement (from 40 to 18 g kg(-1)) of a herd of beef cows grazing tropical pastures of signal grass (Urochloa decumbens). The experiment was carried out in the savanna region of Mato Grosso do Sul, Brazil, during the years 2011 to 2013. Variables analyzed included pregnancy rate, calving interval, weight of calves at weaning, and cost of mineral supplementation. There were no changes in the reproductive parameters of the herd and the weight at weaning of the calves. However, the cost of mineral supplementation was significantly lower when the herd was supplemented with the mineral mix containing only 18 g kg(-1) P. Phosphorus concentration of the forage was analyzed monthly during 1 year and averaged 1.9 ± 0.45 g kg(-1) DM. Thus, it appears possible to reduce P content and cost of mineral supplementation without any adverse effects on the health and productivity of beef cattle herds in the State of Mato Grosso do Sul. However, the final decision should be made based on the clinical-nutritional examination and by constant technical assistance to the farm.