Water-Retaining Polymer and Planting Pit Size on Chlorophyll Index, Gas Exchange and Yield of Sour Passion Fruit with Deficit Irrigation.

Water availability is a limiting factor for the cultivation of sour passion fruit. Soil management techniques and the use of water-retaining polymers can increase soil water retention, reducing the frequency of irrigation in the crop. In this context, the objective of the research was to evaluate the gas exchange, the chlorophyll index, and the yield of the sour passion fruit cv. BRS GA1 as a function of irrigation depths, pit volumes, and doses of water-retaining polymer. The experiment was carried out in randomized blocks, in plots subdivided in a 2 × (2 × 5) arrangement, with irrigation depths of 70 and 100% of the crop evapotranspiration (ETc) as the main plot, the subplots with the volumes of pit of 64 and 128 dm3, and doses of the water-retaining polymer of 0, 0.5, 1.0, 1.5, and 2.0 g dm-3. The interaction of irrigation depths × pit volumes × doses of water-retaining polymer influences chlorophyll indexes, gas exchange, and water productivity, with positive impacts on yield of the sour passion fruit. The water depth of 70% of ETc increased the yield of sour passion fruit, in pits of 64 dm3. The application of doses of up to 1.1 g dm-3 of the water-retaining polymer and irrigation with water of 70% of ETc is recommended, and a dose of 2.0 g dm-3 of the water-retaining polymer in a pit volume of 128 dm3, associated with an irrigation depth of 100% ETc causes stress in sour passion fruit plants due to excess water.

The Effects of Irrigation Water Salinity on the Synthesis of Photosynthetic Pigments, Gas Exchange, and Photochemical Efficiency of Sour Passion Fruit Genotypes.

The objective of this study was to evaluate the synthesis of photosynthetic pigments, gas exchange, and photochemical efficiency of sour passion fruit genotypes irrigated with saline water under the conditions of the semi-arid region of Paraíba state, Brazil. The experiment was conducted at the experimental farm in São Domingos, PB. A randomized block design was adopted, in a 5 × 3 factorial scheme, with five levels of electrical conductivity of irrigation water-ECw (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1)-and three genotypes of sour passion fruit (Gigante Amarelo-'BRS GA1'; Sol do Cerrado-'BRS SC1'; and Catarina-'SCS 437'. The increase in the electrical conductivity of irrigation water negatively affected most of the physiological characteristics of the sour passion fruit at 154 days after transplanting. Significant differences were observed between sour passion fruit genotypes when its tolerance was subjected to the salinity of irrigation water. There was an increase in the percentage of damage to the cell membrane with the increase in the electrical conductivity of irrigation water, with maximum values of 70.63, 60.86, and 80.35% for the genotypes 'BRS GA1', 'BRS SC1', and SCS 437', respectively, when irrigated with water of 3.5 dS m-1. The genotype 'BRS Sol do Cerrado' showed an increase in the synthesis of photosynthetic pigments when irrigated with water of 3.5 dS m-1, with maximum values estimated at 1439.23 µg mL-1 (Chl a); 290.96 µg mL-1 (Chl b); 1730.19 µg mL-1 (Chl t); and 365.84 µg mL-1 (carotenoids). An increase in photosynthetic efficiency parameters (F0, Fm, and Fv) of the genotype 'BRS Gigante Amarelo' was observed when cultivated with water with high electrical conductivity (3.5 dS m-1).

Foliar Applications of Salicylic Acid on Boosting Salt Stress Tolerance in Sour Passion Fruit in Two Cropping Cycles.

Brazil stands out as the largest producer of sour passion fruit; however, the water available for irrigation is mostly saline, which can limit its cultivation. This study was carried out with the objective of evaluating the effects of salicylic acid in the induction of tolerance in sour passion fruit to salt stress. The assay was conducted in a protected environment, using a completely randomized design in a split-plot scheme, with the levels of electrical conductivity of the irrigation water (0.8, 1.6, 2.4, 3.2, and 4.0 dS m-1) considering the plots and concentrations of salicylic acid (0, 1.2, 2.4, and 3.6 mM) the subplots, with three replications. The physiological indices, production components, and postharvest quality of sour passion fruit were negatively affected by the increase in the electrical conductivity of irrigation water, and the effects of salt stress were intensified in the second cycle. In the first cycle, the foliar application of salicylic acid at concentrations between 1.0 and 1.4 mM partially reduced the harmful effects of salt stress on the relative water content of leaves, electrolyte leakage, gas exchange, and synthesis of photosynthetic pigments, in addition to promoting an increase in the yield and quality parameters of sour passion fruit.

Potassium fertilization as salt stress attenuator in sour passion fruit / Adubação potássica como atenuante do estresse salino em maracujazeiro-azedo

Water scarcity associated with irregular rainfall in the semi-arid region of Northeastern Brazil stands out as a limiting factor for agricultural production. Thus, the use of waters with high concentration of salts is an alternative to expand irrigated agriculture in this region. In this context, this study evaluated the water status, intercellular electrolyte leakage, photosynthetic pigments, and gas exchange of 'BRS SC1' sour passion fruit as a function of irrigation with water of different levels of salinity and potassium doses. The experiment was carried out in pots adapted as drainage lysimeters under field conditions at the Experimental Farm of the Federal University of Campina Grande in São Domingos - PB, Brazil. The experimental design was randomized blocks, in a 5 × 4 factorial scheme, whose treatments were obtained by combining two factors: five levels of electrical conductivity of irrigation water - ECw (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1), associated with four potassium doses (60, 80, 100, and 120% of the recommendation), with three replicates. Water with electrical conductivity greater than 0.3 dS m-1 reduced the relative water content, chlorophyll a and chlorophyll b contents, CO2 assimilation rate, and instantaneous water use efficiency of 'BRS SC1' sour passion fruit plants. The estimated potassium dose of 85% of the recommendation (equivalent to 293 g per plant per year) mitigated the deleterious effects of salt stress on stomatal conductance, transpiration, internal CO2 concentration, and instantaneous carboxylation efficiency of passion fruit 'BRS SC1'.

Water scarcity associated with irregular rainfall in the semi-arid region of Northeastern Brazil stands out as a limiting factor for agricultural production. Thus, the use of waters with high concentration of salts is an alternative to expand irrigated agriculture in this region. In this context, this study evaluated the water status, intercellular electrolyte leakage, photosynthetic pigments, and gas exchange of 'BRS SC1' sour passion fruit as a function of irrigation with water of different levels of salinity and potassium doses. The experiment was carried out in pots adapted as drainage lysimeters under field conditions at the Experimental Farm of the Federal University of Campina Grande in São Domingos - PB, Brazil. The experimental design was randomized blocks, in a 5 × 4 factorial scheme, whose treatments were obtained by combining two factors: five levels of electrical conductivity of irrigation water - ECw (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1), associated with four potassium doses (60, 80, 100, and 120% of the recommendation), with three replicates. Water with electrical conductivity greater than 0.3 dS m-1 reduced the relative water content, chlorophyll a and chlorophyll b contents, CO2 assimilation rate, and instantaneous water use efficiency of 'BRS SC1' sour passion fruit plants. The estimated potassium dose of 85% of the recommendation (equivalent to 293 g per plant per year) mitigated the deleterious effects of salt stress on stomatal conductance, transpiration, internal CO2 concentration, and instantaneous carboxylation efficiency of passion fruit 'BRS SC1'.

Productivity and mortality, of Fusarium oxysporum f. sp. passiflorae in passionfruit vines brs rubi do cerrado, grafted onto different rootstocks

The use of scions grafted onto passionfruit vines has become an alternative to their cultivation in areas with a history of fusariosis. However, the combinations between the graft and rootstock can influence on the productivity and longevity of the passionfruit farm. The objective was to evaluate the productivity and mortality of the passionfruit cv BRS Rubi do Cerrado grafted onto three species of rootstock in Terra Nova do Norte-MT. The design was a randomized block with 4 treatments BRS Rubi do Cerrado (BRS-RC) seedling plant along with those grafted onto three species of rootstock: Passiflora gibertii, Passiflora alata; Passiflora nitida. The scions were grafted through a cleft graft and the planting performed after 70 days. The evaluation was made of the total number and weight of the fruits, along with productivity and mortality of plants. Results showed that the BRS-RC, grafted onto P. gibertii and P. nitida, presented the best performance for weight of fruits, number of fruits and productivity. The cultivar that was grafted onto different rootstocks presented higher productivity in regards to the seedling plant. The BRS-RC, grafted onto P. nitida, presented a zero-mortality rate over the 16.5 months of cultivation. The rootstocks P. nitida and P. gibertii were superior to those of P. alata in reducing mortality in the passionfruit vine by Fusarium oxysporum f. sp. passiflorae. The confirmation was made of the pathogen Fusarium oxysporum f. passiflorae isolated in the experimental area in scions of the BRS-RC inoculated at 70 days of age.

Agronomic assessment of 32 sour passionfruit genotypes in federal district

The production of passion fruit is important in Brazil. In order to contribute to the development of the most promising cultivars of passion fruit, this study aimed to evaluate the agronomic performance of 32 genotypes of passion fruit in Federal District of Brazil, and to estimate genetic parameters for use in breeding programs. Thirty-two genotypes were used in a randomized block design, with eight plants per plot and four replications. The experiment was conducted in field. Twenty-eight harvests were performed, and the variables analyzed were: productivity estimated, total number of fruits per hectare, average fruit weight and these characteristics following classification of fruits in five categories. The genotypes that presented the highest total yield estimated were MAR20 # 23, AR 01 and PLANTA 7. For industrial purposes, genotypes MAR 20 # 21 and BRS Gigante Amarelo were superior. For fresh consumption, the genotypes with the best performance were PLANT 7, AR 01 and MSC. Total productivity estimated and total number of fruits per hectare in the first-class classification showed high values of heritability and CVg/CVe ratio. These results indicate a favorable condition for selection.

Postharvest quality of yellow passion fruit produced in soil with bovine biofertilizer and nitrogen.

The use of soil conditioners as bovine biofertilizer associated with mineral fertilization affect the physical and physicochemical quality of passion fruit. For fruit growth, post-harvest quality is crucial for production chain development, as it is the characteristic most used by the fresh consumption market for this fruit. In this sense, an experiment was carried out to investigate the effects of doses of bovine biofertilizer in the soil with and without nitrogen fertilization in the cultivation of yellow passion fruit. A randomized block design was adopted, with three replications in a 5×2 factorial scheme, referring to five doses of liquid bovine biofertilizer (B) diluted in water (A): 0% - control (0B + 4A); 25% (1B + 3A); 50% (2B + 2A); 75% (3B + 1A); and 100% (4B + 0A) with and without nitrogen fertilization applied to the soil. Urea was the nitrogen source used in this study. A total of 10 g plant-1 of N was applied monthly at 30 and 60 days after transplanting, and after that age, 20 g plant-1 was applied until the end of harvest. During the final phase of production and ripening, twelve fruits were harvested from each treatment in physiological maturation for physical and physicochemical characterization. The following analyses were performed: longitudinal diameter, transversal diameter, number of seeds per fruit, peel firmness, pulp yield, fruit peel percentage, pulp pH, soluble solids content; titratable acidity and soluble solids content/titratable acidity ratio. Data underwent analysis of variance by the F test means for nitrogen were compared by Tukey's test and means for bovine biofertilizer, by regression. Nitrogen enhances the positive effect of bovine biofertilizer on the postharvest quality of yellow passion fruit. The association of biofertilizer and nitrogen improves fruit quality in comparison to plants without these inputs, except for pulp yield and fruit peel percentage, which suffered isolated effects from the factors. High doses of biofertilizer, above 75 and 100%, reduce soluble solids content and increase titratable acidity. The bovine biofertilizer has promising effects, but it does not replace nitrogen fertilization on the postharvest quality of yellow passion fruit.

Quantum yield, chlorophyll, and cell damage in yellow passion fruit under irrigation strategies with brackish water and potassium / Rendimento quântico, clorofila e dano celular em maracujazeiro-amarelo sob estratégias de irrigação com água salobra e potássio

The occurrence of water sources with high concentrations of salts in the semiarid region of Northeast Brazil stands out as a limiting factor in the cultivation of irrigated yellow passion fruit. Thus the search for irrigation strategies with brackish water is fundamental for the sustainability of irrigated crops. The objective of the present study was to determine the quantum yield, chlorophyll levels, and cell damage in yellow passion fruit cultivated under different irrigation strategies with brackish water and potassium fertilization. The experiment was conducted under field conditions in São Domingos - PB, Brazil, adopting a randomized block design in a 6×2 factorial scheme, consisting of six strategies of use of brackish water applied in the different phenological stages of crop and two doses of potassium (60 and 100% of the recommendation), with four replicates. Two levels of irrigation water salinity were used, with low (1.3 dS m-1) and high electrical conductivity (4.0 dS m-1). The potassium dose of 100% recommendation corresponds to the application of 345 g of K2O per plant per year. The use of brackish water (4.0 dS m-1) increased chlorophyll fluorescence and negatively affected the photosynthetic pigments of yellow passion fruit, regardless of the phenological stage. Salt stress intensified intercellular electrolyte leakage in passion fruit plants under higher dose of potassium. Our results show that fertilization equivalent to 100% of the potassium recommendation is excessive for yellow passion fruit 'BRS GA1' in the tropical semiarid region under salt stress conditions.

A ocorrência de mananciais com altas concentrações de sais no semiárido nordestino destaca-se como fator limitante no cultivo do maracujazeiro-amarelo irrigado. Assim, a busca por estratégias de irrigação com água salobra é fundamental para a sustentabilidade da agricultura irrigada. O objetivo deste trabalho foi determinar o rendimento quântico, os teores de clorofila e dano celular em maracujazeiro-amarelo cultivado sob diferentes estratégias de irrigação com água salobra e adubação potássica. O experimento foi conduzido em condições de campo em São Domingos - PB, Brasil, adotando-se o delineamento em blocos casualizados em esquema fatorial 6×2, correspondendo a seis estratégias de uso de água salobra aplicadas nos diferentes estádios fenológicos da cultura e duas doses de potássio (60 e 100% da recomendação), com quatro repetições. Foram utilizados dois níveis de salinidade da água de irrigação, com baixa (1,3 dS m-1) e alta condutividade elétrica (4,0 dS m-1). A dose de potássio de 100% corresponde à aplicação de 345 g de K2O por planta por ano. O uso de água salobra (4,0 dS m-1) aumentou a fluorescência da clorofila e afetou negativamente os pigmentos fotossintéticos do maracujá-amarelo, independente do estádio fenológico. O estresse salino intensificou o extravasamento de eletrólitos em plantas de maracujazeiro adubadas sob alta dose de potássio. Os resultados mostram que a adubação equivalente a 100% da recomendação de potássio é excessiva para o cultivo do maracujazeiro-amarelo 'BRS GA1' no semiárido tropical, sob condições de estresse salino.

Determination of changes in physicochemical and sensory characteristics of purple passion fruit with the application of different packaging systems, including an ethylene scavenger additive.

To achieve a suitable packaging configuration, it is important first to determine the physicochemical characteristics related to the packaged product. In this study, the physicochemical characterization of fresh purple passion fruits of three different ripening stages was carried out to determine key variables for the packaging, such as O2 consumption and CO2 -ethylene production rates. Subsequently, intermediate-ripe fruits were packaged for 21 days at 6 °C under three packaging conditions: Xtend® perforated bags, low-density polyethylene (LDPE) bags, and LDPE bags with a novel ethylene scavenger active additive (ESAA). It was observed that an equilibrium modified atmosphere was formed in the packages. For the Xtend® bags, the highest values of O2 (yo2  = 0.184 to 0.192) and lowest of CO2 (yco2  = 0.033 to 0.041) were reached, whereas for the LDPE bags with ESAA these values were moderate. In the case of ethylene, the LDPE bags showed the highest levels in the headspace (26 to 31 ppm), whereas the lowest levels were obtained in the LDPE bags with additive (2 to 4 ppm). These levels resulted in a delay in the ripening of the fruits during storage, which was verified through a sensory acceptability test that was carried out on the juice extracted from the fruits. In this sensory test, panelists identified similar characteristics between the fruits packaged with ESAA and the Xtend® bags, regarding the control fruits. The LDPE bags with the ethylene scavenger performed satisfactorily and can considerably delay the ripening, which may result in longer shelf life and conservation of fresh purple passion fruits. PRACTICAL APPLICATION: This work presents a novel packaging proposal that reduces oxygen and ethylene levels in contact with purple passion fruits. Our proposed active packaging can be used to increase the fruit shelf life by improving its conservation conditions throughout the chain of storage, transport, and distribution in the market. With this, it will be possible to reduce the fruit's losses due to senescence and to reduce the substrate consumption by using a more effective packaging system.

Cost reduction and quality enhancement in passion fruit seedlings using alternative substrate / Redução de custo e aumento da qualidade de mudas de maracujá azedo usando substrato alternativo

Obtaining vigorous, quality seedlings is a complex task because it involves multiple physical, chemical and biological factors, with the substrate being a fundamental of this process. Therefore, this study proposes to evaluate different formulations of substrate mixtures and identify which provides better emergence vigor and shoot and root growth in passion fruit (Passiflora edulis Sims). The experiment was laid out in a completely randomized design consisting of 23 substrates with different proportions of Vivatto® and Osmocote®, washed sand, soil, fine coal, coarse coal, enriched hydrogel and enriched vermiculite, with and without the addition of NPK, which were distributed into four replications of four plants per plot. To enrich the hydrogel and vermiculite, foliar fertilizer was used. Emergence vigor was evaluated throughout the seedling emergence period and seedling growth parameters were observed at 60 days after sowing. The data were subjected to multivariate analysis and the groups formed were subjected to analysis of variance. Based on principal component analysis (PCA), four groups were identified. The group composed only of soil + coarse coal and enriched hydrogel (2:2:1) was considered the best treatment, as it favored most of the vegetative growth and emergence vigor traits. Soil plus sand with the addition of NPK was not recommended to grow passion fruit seedlings in any of the proportions used, as there was no emergence.

A obtenção de mudas com qualidade e vigor é complexa, pois envolve múltiplos fatores físicos, químicos e biológicos sendo o substrato fundamental neste processo, assim, o objetivo deste trabalho foi avaliar diferentes formulações de substrato e identificar qual proporciona melhor vigor de emergência e crescimento de parte aérea e raízes de maracujá amarelo (Passiflora edulis Sims). O delineamento experimental foi inteiramente casualizado, composto por 23 substratos, em diferentes misturas de Vivatto®, Osmocote®, areia lavada, solo, resíduo de carvão fino, carvão grosso, hidrogel enriquecido e vermiculita enriquecida com adubo foliar, além de tratamentos com e sem adição de NPK, distribuídos em quatro repetições de quatro plantas por parcela. O vigor de emergência foi avaliado durante o período de emergência das plântulas e os parâmetros de crescimento das plântulas foram observados aos 60 dias após a semeadura. Os dados foram submetidos à análise multivariada e os grupos formados foram submetidos à análise de variância. Com base na análise de componentes principais (PCA), quatro grupos foram identificados. O grupo composto apenas de solo + carvão vegetal e hidrogel enriquecido (2:2:1), foi considerado o melhor tratamento, pois favoreceu a maioria das características de crescimento vegetativo e vigor de emergência. O solo mais a areia com a adição de NPK não foi recomendado para a produção de mudas de maracujá azedo em nenhuma das proporções utilizadas, pois não houve emergência.

Foliar application of H2O2 as salt stress attenuator in 'BRS Rubi do Cerrado' sour passion fruit / Aplicação foliar de H2O2 como atenuante do estresse salino em maracujazeiro-azedo 'BRS Rubi do Cerrado'

Irrigation with saline water causes a reduction in yield, especially in semi-arid regions. Cultivation strategies have been developed to mitigate salt stress on plants, such as the use of hydrogen peroxide. The objective of this study was to evaluate the attenuating effect of hydrogen peroxide on the gas exchange and growth of 'BRS Rubi do Cerrado' sour passion fruit cultivated under irrigation with saline water. The design was completely randomized in split-plot plots, with water salinity levels ECw (0.6, 1.2, 1.8, 2.4, and 3.0 dS m-1) considered the plots and the concentrations of hydrogen peroxide H2O2 (0, 15, 30, and 45 µM) considered the subplots, with three replicates. Gas exchange (stomatal conductance, transpiration, CO2 assimilation rate, intercellular CO2 concentration, instantaneous water use efficiency, and instantaneous carboxylation efficiency), and absolute and relative growth rates in stem diameter were evaluated. An increase in irrigation water salinity from 0.6 dS m-1 reduced gas exchange, and exogenous application of hydrogen peroxide did not promote a significant effect on gas exchange. However, foliar application of hydrogen peroxide at 15 µM increased the growth of 'BRS Rubi do Cerrado' sour passion fruit.(AU)

A irrigação com águas salinas causa redução na produtividade, principalmente nas regiões semiáridas. Estratégias de cultivo vêm sendo desenvolvidas, a exemplo uso de peróxido de hidrogênio para mitigar o estresse salino sobre as plantas. Objetivou-se avaliar o efeito atenuante do peróxido de hidrogênio sobre as trocas gasosas e o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado' cultivado sob irrigação com águas salinas. O delineamento foi inteiramente casualizados em parcelas subdivididas, sendo os níveis de salinidade da água - CEa (0,6, 1,2, 1,8, 2,4 e 3,0 dS m-1) consideradas as parcelas, e as concentrações de peróxido de hidrogênio ­ H2O2 (0, 15, 30 e 45 µM) as subparcelas, com três repetições. Foram avaliadas as trocas gasosas (condutância estomática, transpiração, taxa de assimilação de CO2, concentração intercelular de CO2, eficiência instantânea no uso da água e eficiência instantânea de carboxilação), e a taxa de crescimento absoluto e relativo do caule. O aumento na salinidade da água de irrigação a partir da salinidade de 0,6 dS m-1 reduziu as trocas gasosas; a aplicação exógena de peróxido de hidrogênio não promoveu efeito significativos sob as trocas gasosas. A aplicação foliar de 15 µM de peróxido de hidrogênio aumentou o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado'.(AU)

Cost reduction and quality enhancement in passion fruit seedlings using alternative substrate / Redução de custo e aumento da qualidade de mudas de maracujá azedo usando substrato alternativo

Obtaining vigorous, quality seedlings is a complex task because it involves multiple physical, chemical and biological factors, with the substrate being a fundamental of this process. Therefore, this study proposes to evaluate different formulations of substrate mixtures and identify which provides better emergence vigor and shoot and root growth in passion fruit (Passiflora edulis Sims). The experiment was laid out in a completely randomized design consisting of 23 substrates with different proportions of Vivatto® and Osmocote®, washed sand, soil, fine coal, coarse coal, enriched hydrogel and enriched vermiculite, with and without the addition of NPK, which were distributed into four replications of four plants per plot. To enrich the hydrogel and vermiculite, foliar fertilizer was used. Emergence vigor was evaluated throughout the seedling emergence period and seedling growth parameters were observed at 60 days after sowing. The data were subjected to multivariate analysis and the groups formed were subjected to analysis of variance. Based on principal component analysis (PCA), four groups were identified. The group composed only of soil + coarse coal and enriched hydrogel (2:2:1) was considered the best treatment, as it favored most of the vegetative growth and emergence vigor traits. Soil plus sand with the addition of NPK was not recommended to grow passion fruit seedlings in any of the proportions used, as there was no emergence.(AU)

A obtenção de mudas com qualidade e vigor é complexa, pois envolve múltiplos fatores físicos, químicos e biológicos sendo o substrato fundamental neste processo, assim, o objetivo deste trabalho foi avaliar diferentes formulações de substrato e identificar qual proporciona melhor vigor de emergência e crescimento de parte aérea e raízes de maracujá amarelo (Passiflora edulis Sims). O delineamento experimental foi inteiramente casualizado, composto por 23 substratos, em diferentes misturas de Vivatto®, Osmocote®, areia lavada, solo, resíduo de carvão fino, carvão grosso, hidrogel enriquecido e vermiculita enriquecida com adubo foliar, além de tratamentos com e sem adição de NPK, distribuídos em quatro repetições de quatro plantas por parcela. O vigor de emergência foi avaliado durante o período de emergência das plântulas e os parâmetros de crescimento das plântulas foram observados aos 60 dias após a semeadura. Os dados foram submetidos à análise multivariada e os grupos formados foram submetidos à análise de variância. Com base na análise de componentes principais (PCA), quatro grupos foram identificados. O grupo composto apenas de solo + carvão vegetal e hidrogel enriquecido (2:2:1), foi considerado o melhor tratamento, pois favoreceu a maioria das características de crescimento vegetativo e vigor de emergência. O solo mais a areia com a adição de NPK não foi recomendado para a produção de mudas de maracujá azedo em nenhuma das proporções utilizadas, pois não houve emergência.(AU)

Assessment of resistance of hybrids and lineages of passion fruit to bacteriosis under protected cultivation / Avaliação da resistência de híbridos e linhagens de maracujazeiro à bacteriose sob cultivo protegido

Yield and longevity of yellow passion fruit have been reduced by diseases such as the bacterial spot caused by Xanthomonas axonopodis pv. passiflorae. Genetic resistance has been confirmed as the most efficient and economical correct option to minimize this disease problem. Aiming at it, the objective of this research was to evaluate the incidence, severity and progression of the disease in 12 genotypes of sour passion fruit, in seedling stage in nursery greenhouse after inoculation of Xanthomonas axonopodis pv. passiflorae. The inoculation was performed with an isolate collected in the Pipiripau Rural Nucleus, Brasilia-DF, named UnB-1397 (1x106 CFU/mL), through induction of injuries. There were performed four assessments, with interval of 7 days except the first which was performed 11 days after inoculation. The incidence was estimated by the percentage of plants affected. To evaluate the severity, it was used the diagrammatic scale validated by Costa et al. (2018), with adaptations, using the measurement of the affected area by necrotic lesions on the leaf. All genotypes were susceptible to bacteriosis, 5 being considered moderately susceptible: F1BRS Pérola do Cerrado x Rosa Intenso, Mar20#21, Mar20#15b, Mar20#24xMar20#40 and FB200PL4R2 x Mar20#2005, with a mean of severity ranging from 11 to 25% of injured area in leaves.

A produtividade e a longevidade dos pomares de maracujazeiro-azedo têm sido comprometidas em razão de doenças como a bacteriose, causada por Xanthomonas axonopodis pv. passiflorae. A resistência genética tem se confirmado como a opção mais eficiente e econômica para minimizar tal problema. Dessa forma, o objetivo deste trabalho foi avaliar a reação de 12 genótipos de maracujazeiro, em fase de mudas, sob cultivo protegido, à Xanthomonas axonopodis pv. passiflorae. A inoculação com isolado denominado UnB-1397 (1x106 CFU/mL),coletado no Núcleo Rural de Pipiripau, Brasília-DF, se deu pela indução de ferimentos. Foram realizadas 4 avaliações, com intervalo de 7 dias, sendo a primeira avaliação realizada 11 dias após a inoculação. A incidência foi estimada pela porcentagem de plantas afetadas. Para avaliação da severidade, foi utilizada escala diagramática validada por Costa et al. (2018), com adaptações, utilizando-se a mensuração da área foliar atingida por lesões nas folhas. Todos os genótipos se mostraram suscetíveis à bacteriose, sendo 5 considerados moderadamente suscetíveis: F1 BRS Pérola do Cerrado x Rosa Intenso, Mar20#21, Mar20#15b, Mar20#24 x Mar20#40 e FB200PL4R2 x Mar20#2005, apresentando uma média de severidade que variou de 11 a 25% de área ou tecidos foliares lesionados.

Standard area diagram set for scab quantification in trilobed leaves of the sour passion fruit / Escala diagramática para a quantificação da verrugose em folhas trilobadas do maracujazeiro azedo

The study aimed to develop and validate a standard area diagram set (SADs) to improve accuracy, precision, and reproducibility in the assessment of scab severity in trilobed leaves of the sour passion fruit. The SADs comprised six severity levels (1%; 3%; 6%; 11%; 21%; and 33%). The SADs increased the accuracy of the estimates by reducing the constant and systematic errors, and the tendency to overestimate the actual severity, limited the number of evaluators who presented this trend, in addition to increasing the bias correction factor. The inexperienced evaluators were the ones that benefited the most from the SADs use. Once the SADs was introduced, 80% and 100% evaluators gave estimates free from constant and systematic errors, respectively. The improvement in precision was verified by the increase in the coefficient of determination and Lins concordance correlation coefficient, by reducing absolute errors and increasing the reproducibility of the estimates.(AU)

O objetivo deste estudo foi desenvolver e validar uma escala diagramática para melhorar a acurácia, precisão e reprodutibilidade na avaliação da severidade da verrugose em folhas trilobadas do maracujazeiro azedo. A escala apresentou seis níveis de severidade (1; 3; 6; 11; 21 e 33%) e aumentou a acurácia das estimativas, reduzindo os erros constantes e sistemáticos, a tendência de superestimação em relação aos valores de severidade real e o número de avaliadores que apresentavam essa tendência, além de aumentar o fator de correção do desvio. Os avaliadores inexperientes foram os mais beneficiados pela utilização da escala, sendo que 80% e 100% destes avaliadores apresentaram estimativas livres de erros constantes e sistemáticos, respectivamente, quando a escala foi empregada. O aumento da precisão foi verificado pelo incremento dos coeficientes de determinação e de concordância de Lin, redução dos erros absolutos e aumento da reprodutibilidade das estimativas.(AU)

Production of Probiotic Passion Fruit (Passiflora edulis Sims f. flavicarpa Deg.) Drink Using Lactobacillus reuteri and Microencapsulation via Spray Drying.

Probiotic foods offer many benefits to human health, causing increased interest in the development of new food products that exploit such benefits. However, traditional dairy foods are being replaced by other non-dairy foods to provide additional sources of benefits provided by bioactive molecules. Therefore, the objective of the present work was to study the production process of a probiotic fruit drink and then microencapsulate the probiotic pulp to stabilize the drink further. Passion fruit pulp (Passiflora edulis Sims f. flavicarpa Deg.) was fermented with Lactobacillus reuteri under different temperature conditions in combination with different pHs to find the best fermentation conditions. Different from dairy sources, the optimal conditions for the growth of Lactobacillus reuteri in the passion fruit pulp were found to be 30 °C at pH 3.18, where phenolic compounds could also be used as a secondary metabolic pathway. Spray-drying was performed using different conditions for microencapsulation. Process yields and Lactobacillus reuteri survival showed the dependency of droplet sizes, whereas phenolic compound retention was increased when higher amounts of gelatin were used. Therefore, the development of a new food product comprising a powdered fruit pulp rich in probiotic and phenolic compounds was possible.

Passion fruit (Passiflora edulis) leaf extract modulates the oxidative metabolism of rat peritoneal neutrophils in a model of inflammation

This study was conducted to evaluate the effect of extracts of Passiflora edulis Sims leaves on the oxidative metabolism of rat peritoneal neutrophils using a model of acute inflammation. The extract was obtained by maceration in 70% ethanol, evaporation under reduced pressure and lyophilisation. Total phenolic content (TP) was determined by the Folin-Ciocalteu assay. The P. edulis extract, in different doses, was administered by gavage 1 h prior to inflammation induction by carrageenan (8 mg/kg, i.p.); five hours later, the neutrophils were obtained by intraperitoneal lavage. The tests performed in neutrophils were cytochrome C and chemiluminescence assay as well as myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. The administration of the extract reduced the number of neutrophils recruited to the site of inflammation; however, the extract did not alter the activity of NADPH oxidase as well as SOD activity in these cells. The MPO and CAT activities in peritoneal neutrophils of rat treated with extract was lower than in the control group, and the GPx activity was increased. Based on the experimental model utilised, the anti-inflammatory potential of P. edulis leaf extract could be related to the presence of phenolic compounds in the extract.

Standard area diagram set for scab quantification in trilobed leaves of the sour passion fruit / Escala diagramática para a quantificação da verrugose em folhas trilobadas do maracujazeiro azedo

ABSTRACT: The study aimed to develop and validate a standard area diagram set (SADs) to improve accuracy, precision, and reproducibility in the assessment of scab severity in trilobed leaves of the sour passion fruit. The SADs comprised six severity levels (1%; 3%; 6%; 11%; 21%; and 33%). The SADs increased the accuracy of the estimates by reducing the constant and systematic errors, and the tendency to overestimate the actual severity, limited the number of evaluators who presented this trend, in addition to increasing the bias correction factor. The inexperienced evaluators were the ones that benefited the most from the SADs use. Once the SADs was introduced, 80% and 100% evaluators gave estimates free from constant and systematic errors, respectively. The improvement in precision was verified by the increase in the coefficient of determination and Lin's concordance correlation coefficient, by reducing absolute errors and increasing the reproducibility of the estimates.

RESUMO: O objetivo deste estudo foi desenvolver e validar uma escala diagramática para melhorar a acurácia, precisão e reprodutibilidade na avaliação da severidade da verrugose em folhas trilobadas do maracujazeiro azedo. A escala apresentou seis níveis de severidade (1; 3; 6; 11; 21 e 33%) e aumentou a acurácia das estimativas, reduzindo os erros constantes e sistemáticos, a tendência de superestimação em relação aos valores de severidade real e o número de avaliadores que apresentavam essa tendência, além de aumentar o fator de correção do desvio. Os avaliadores inexperientes foram os mais beneficiados pela utilização da escala, sendo que 80% e 100% destes avaliadores apresentaram estimativas livres de erros constantes e sistemáticos, respectivamente, quando a escala foi empregada. O aumento da precisão foi verificado pelo incremento dos coeficientes de determinação e de concordância de Lin, redução dos erros absolutos e aumento da reprodutibilidade das estimativas.

Development and validation of a standard area diagram set as assessment aid for estimating the severity of bacterial spot on tri-lobed leaves of yellow passion fruit / Desenvolvimento e validação de escala diagramática como auxílio na avaliação da severidade da bacteriose em folhas trilobadas do maracujazeiro azedo

Bacterial spot (Xanthomonas axonopodis pv. passiflorae) significantly reduces yellow passion fruit (Passiflora edulis Sims) yield and longevity. A standard area diagram set (SADs) for severity assessment of bacterial spot on tri-lobed leaves of yellow passion was developed and validated in this study. The SADs consisted of eight severity levels (2; 4; 9; 18; 35; 58; 80; and 94%). For its validation, 20 raters, who initially estimated the disease severity without the aid of the SADs, were divided into four groups (G1 and G3, inexperienced; G2 and G4, experienced). Subsequently, G1 and G2 performed the second evaluation without the SADs, and G3 and G4 completed the second evaluation with the proposed SADs. The accuracy and precision of the assessments were determined by simple linear regression and by the Lin's concordance correlation coefficient (LCCC). The proposed SADs allowed accurate and precise quantification of bacterial spot severity, increasing the agreement between estimated and actual values. Inexperienced raters benefited the most from the use of the SADs. The increase in accuracy and precision in the non-aided groups, when present, was less pronounced than those increments observed in the SADs-aided groups. The LCCC confirmed the increases in accuracy and precision detected by the linear regression analysis.

A bacteriose (Xanthomonas axonopodis pv. passiflorae) reduz significativamente a produção e longevidade do maracujazeiro azedo (Passiflora edulis Sims). Uma escala diagramática para a avaliação da severidade da bacteriose em folhas trilobadas do maracujazeiro azedo foi desenvolvida e validada neste estudo. A escala diagramática apresentou oito níveis de severidade (2; 4; 9; 18; 35; 58; 80 e 94%). Para a sua validação, os 20 avaliadores foram divididos em quatro grupos (G1 e G3, sem experiência; G2 e G4, com experiência), que inicialmente estimaram a severidade da doença sem auxílio da escala. Posteriormente, G1 e G2 fizeram outra avaliação sem escala, e G3 e G4 realizaram a avaliação com a escala proposta. A acurácia e a precisão das estimativas foram determinadas por regressão linear simples e pelo coeficiente de correlação de concordância de Lin (LCCC). A escala diagramática proposta permitiu quantificar a severidade da bacteriose de forma acurada e precisa, aumentando a concordância entre os valores estimados e os reais. Os avaliadores inexperientes foram os mais beneficiados pelo uso da escala. O aumento da acurácia e precisão nos grupos que realizaram dupla avaliação sem escala, quando ocorreu, foi mais discreto que os incrementos observados nos grupos que utilizaram a escala. O LCCC confirmou os incrementos da acurácia e precisão detectados pela análise de regressão linear.

Nitrogen as a mitigator of salt stress in yellow passion fruit seedlings / O nitrogênio como mitigador do estresse salino nas mudas de maracujazeiro amarelo

The poor chemical quality of water, especially in arid and semiarid regions, almost always precludes the practice of irrigated agriculture, thus demanding the adoption of techniques that mitigate the deleterious effects of excess salt on soil and plants. The aim of this research was to evaluate the effectiveness of nitrogen fertilization in mitigating the negative effects of excess salt in irrigation water on the growth of yellow passion fruit seedlings grown in a greenhouse in plastic tubes containing 0.65 dm3 of substrate. The treatments were organized in randomized blocks, in accordance with a 5 × 3 factorial scheme five electrical conductivities of irrigation water (0.3, 1.0, 2.0, 3.0, and 4.0 dS m-1) combined with three levels of nitrogen fertilizer (no nitrogen fertilization and 150 mg dm-3 of N derived from either ammonium sulfate or urea). Evaluations were performed 80 days after sowing and consisted of measuring the seedling height, stem diameter, number of leaves, leaf area, leaf nitrogen content, leaf concentration of chlorophyll a and b and total chlorophyll, specific leaf area, leaf area ratio, and Dickson quality index. An increase in the electrical conductivity of irrigation water hindered the production of yellow passion fruit seedlings. Nitrogen fertilization, with urea or ammonium sulfate, mitigated the effects of irrigation water salinity and favored the...(AU)

A baixa qualidade química da água, especialmente nas regiões áridas e semiáridas, quase sempre impede a prática da agricultura irrigada, exigindo a adoção de técnicas que atenuem os efeitos deletérios do excesso de sal no solo e nas plantas. O objetivo desta pesquisa foi avaliar a eficiência da adubação nitrogenada na mitigação dos efeitos negativos do excesso de sal na água de irrigação, sobre o crescimento de mudas de maracujazeiro amarelo, produzidas em tubetes contendo 0,65 dm3 de substrato em uma estufa telada. Os tratamentos, distribuídos em blocos ao acaso, obedeceu um esquema fatorial de 5 × 3 cinco condutividade elétrica da água de irrigação (0,3; 1,0; 2,0; 3,0; e 4,0 dS m-1) combinadas níveis de adubação nitrogenada (sem adubação nitrogenada e com aplicação 150 mg dm-3 de N utilizando sulfato de amônio ou ureia). As avaliações foram realizadas aos 80 dias após a semeadura e consistiram em medir a altura da muda, o diâmetro do caule, o número de folhas, a área foliar, o teor foliar de nitrogênio, os índices foliares de clorofila a e b e clorofila total, a área foliar específica, a razão de área foliar e o Índice de qualidade de Dickson. O aumento na condutividade elétrica da água de irrigação reduziu a qualidade das mudas de maracujazeiro amarelo. A adubação nitrogenada, com ureia ou sulfato de amônio, atenuou os efeitos da salinidade da água de irrigação...(AU)

Produção de mudas de maracujazeiro amarelo em substratos contendo resíduos vegetais / Production of yellow passion fruit seedlings on substrates containing plant residues

Um dos problemas enfrentados pelos produtores de maracujá é a dificuldade de obtenção de mudas de qualidade. Assim, o objetivo desse trabalho foi avaliar diferentes substratos contendo resíduos vegetais na produção de mudas de maracujazeiro amarelo. O delineamento experimental foi em bloco casualizados (DBC) com 10 tratamentos, 3 repetições, 10 plantas por parcelas, totalizando 300 mudas. Os tratamentos foram formulados com resíduos vegetais em mistura com solo em diferentes proporções expressas em volume/volume (v/v) e consistiram de: T1 - resíduo de soja + solo (1:2); T2 -resíduo de soja + solo (1:3); T3 - resíduo de soja + solo (1:4); T4 - resíduo de algodão + solo (1:2); T5 - resíduo de algodão + solo (1:3); T6 -resíduo de algodão + solo (1:4); T7 -resíduo de cana-de-açúcar + solo (1:2); T8 - resíduo de cana-de-açúcar + solo(1:3); T9 - resíduo de cana-de-açúcar + solo (1:4); T10 - solo (testemunha). As mudas foram obtidas através de sementes de maracujazeiro amarelo (Passiflora edulis Sims f. flavicarpa Deg.) cultivar ‘FB200 Yellow Master’. As características avaliadas foram: altura, diâmetro do caule, número de folhas, massa fresca e seca do sistema radicular e da parte aérea. O substrato formulado com resíduo de cana-de-açúcar + solo (1:2) proporcionou maior crescimento das mudas de maracujazeiro, sendo recomendado para a produção de mudas de Maracujazeiro amarelo.

One of the problems faced by passion fruit producers is the difficulty of obtaining quality seedlings, the substrate being an important input to ensure the satisfactory growth of the seedlings. Thus, the objective of this study was to evaluate different substrates containing plant residues in the production of yellow passion fruit seedlings. The experimental design was in a randomized block with 10 treatments, 3 replicates, 10 plants per plot, totaling 300 seedlings. The treatments were formulated with vegetal residues in mixture with soil in different proportions expressed in volume/volume (v/v) and consisted of: T1- soybean residue + soil (1:2); T2 - soybean residue + soil (1:3); T3 - soybean residue + soil (1:4); T4 - cotton residue + soil (1:2); + T5 - cotton residue + soil (1:3); T6 - cotton residue + soil (1:4); T7 - sugarcane residue + soil (1:2); T8 - sugarcane residue + soil (1:3); T9 - sugarcane residue + soil (1:4); T10 - soil (control). The seedlings were obtained from yellow passion fruit seeds (Passiflora edulis Sims f. flavicarpa Deg.) cultivar 'FB200 Yellow Master'. The characteristics evaluated were: height, stem diameter, number of leaves, fresh and dry mass of root and shoot. The substrate formulated with sugarcane bagasse + soil (1:2) was provided the highest growth of seedlings, being recommended for the production of Yellow passion fruit seedlings.