Anaphylaxis and systemic mastocytosis caused by Solenopsis invicta stings / Anafilaxia e mastocitose sistêmica ocasionada pela Solenopsis invicta

Indolent systemic mastocytosis is a rare disease characterized by an increased number of mast cells in the bone marrow and other tissues, such as the liver, spleen, lymph nodes, and skin. Patients with indolent systemic mastocytosis and high serum tryptase levels are at risk for Hymenoptera venom-induced anaphylaxis. Hymenoptera venom immunotherapy in patients with specific IgE is safe and effective. While some patients can receive ultra-rush venom immunotherapy with minimal side effects, omalizumab effectively protects against anaphylaxis during the build-up phase.

A mastocitose sistêmica indolente é uma doença rara caracterizada por um número aumentado de mastócitos na medula óssea e em outros tecidos, como fígado, baço, linfonodos e pele. Pacientes com mastocitose sistêmica indolente e altos níveis séricos de triptase correm risco de anafilaxia induzida pelo veneno dos Hymenoptera. A imunoterapia com veneno de himenópteros em pacientes com IgE específica é segura e eficaz. Embora alguns pacientes possam receber imunoterapia com veneno ultrarrápido com efeitos colaterais mínimos, o omalizumabe protegeu efetivamente contra a anafilaxia durante a fase de acúmulo.

Susceptibility of Boll Weevil (Coleoptera: Curculionidae) to Ethiprole, Differential Toxicity Against Selected Natural Enemies, and Diagnostic Concentrations for Resistance Monitoring.

Synthetic insecticide application is one tactic for reducing boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), infestations during the cotton, Gossypium hirsutum L., reproductive stage. We assessed the susceptibility of the boll weevil and its natural enemies to ethiprole (mode of action 2B), a phenylpyrazole insecticide, and diagnostic concentrations of ethiprole indicative of boll weevil susceptibility. Differences in the lethal concentrations of ethiprole were calculated with susceptibility ratios based on LC50 ranging from 2.89- to 10.34-fold relative to a natural susceptible population. The lowest and the highest recommended field rates of ethiprole, 100 and 200 g a.i./ha, produced residues that caused 83.3% and 93.7% mortality of weevils caged with cotton leaves from field-treated plants for 8 d. We found that ethiprole was less toxic than fipronil to the boll weevil parasitoid Bracon vulgaris Ashmead (Hymenoptera: Braconidae) and to the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae), while fipronil was highly toxic to both. Adult earwigs, Euborellia annulipes Lucas (Dermaptera: Anisolabididae), were relatively tolerant to ethiprole and fipronil at the highest field rates. Pooled LC50-and LC95-concentrations of ethiprole calculated from studied populations were used as diagnostic for boll weevil mortality, and the outcome fitted to the expected mortality for boll weevil populations from different locations serving for further control failure assessment. Ethiprole appears to be suitable for boll weevil control with low impact on natural enemy communities.

The assembly and importance of a novel ecosystem: The ant community of coffee farms in Puerto Rico.

Agricultural ecosystems are by their very nature novel and by definition the more general biodiversity associated with them must likewise constitute a novel community. Here, we examine the community of arboreally foraging ants in the coffee agroecosystem of Puerto Rico. We surveyed 20 coffee plants in 25 farms three times in a period of one year. We also conducted a more spatially explicit sampling in two of the farms and conducted a species interaction study between the two most abundant species, Wasmannia auropunctata and Solenopsis invicta, in the laboratory. We find that the majority of the most common species are well-known invasive ants and that there is a highly variable pattern of dominance that varies considerably over the main coffee producing region of Puerto Rico, suggesting an unusual modality of community structure. The distribution pattern of the two most common species, W. auropunctata and S. invicta, suggests strong competitive exclusion. However, they also have opposite relationships with the percent of shade cover, with W. auropunctata showing a positive relationship with shade, while S. invicta has a negative relationship. The spatial distribution of these two dominant species in the two more intensively studied farms suggests that young colonies of S. invicta can displace W. auropunctata. Laboratory experiments confirm this. In addition to the elaboration of the nature and extent of this novel ant community, we speculate on the possibilities of its active inclusion as part of a biological control system dealing with several coffee pests, including one of the ants itself, W. auropunctata.

Nine new RNA viruses associated with the fire ant Solenopsis invicta from its native range.

The red imported fire ant (Solenopsis invicta) escaped its natural enemies when it was introduced into North America in the 1930s from South America. US efforts have focused on discovery of natural enemies, like viruses, to provide sustainable control of the ant. Nine new virus genomes were sequenced from the invasive fire ant Solenopsis invicta using metagenomic RNA sequencing. The virus genomes were verified by Sanger sequencing and random amplification of cDNA ends reactions. In addition to the nine new virus genomes, the previously described Solenopsis viruses were also detected, including Solenopsis invicta virus 1 (SINV-1), SINV-2, SINV-3, SINV-4, SINV-5, and Solenopsis invicta densovirus. The virus sequences came from S. invicta workers, larvae, pupae, and dead workers taken from midden piles collected from across the ant's native range in Formosa, Argentina. One of the new virus genomes (Solenopsis invicta virus 6) was also detected in populations of North American S. invicta. Phylogenetic analysis of the RNA dependent RNA polymerase, the entire nonstructural polyprotein, and genome characteristics were used to tentatively taxonomically place these new virus genome sequences; these include four new species of Dicistroviridae, one Polycipiviridae, one Iflaviridae, one Totiviridae, and two genome sequences that were too taxonomically divergent to be placed with certainty. The S. invicta virome is the best characterized from any ant species and includes 13 positive-sense, single-stranded RNA viruses (Solenopsis invicta virus 1 to Solenopsis invicta virus 13), one double-stranded RNA virus (Solenopsis midden virus), and one double-stranded DNA virus (Solenopsis invicta densovirus). These new additions to the S. invicta virome offer potentially new classical biological control agents for S. invicta.

Pseudacteon Phorid Flies: Host Specificity and Impacts on Solenopsis Fire Ants.

Human commerce has resulted in the spread of the imported fire ants, Solenopsis species, worldwide. Six species of parasitic Pseudacteon phorid flies that are highly host specific to the Solenopsis saevissima complex of Solenopsis fire ants have been successfully released in the southern United States. The presence of Pseudacteon phorid flies, in addition to having direct mortality effects on their host ants, modifies foraging behavior and disrupts interspecific competition between host species and other ant species in the community. Fire ant workers have evolved effective methods to cope with parasitism pressure, which may relieve population-level impacts of introduced phorid flies. This review focuses on the mechanisms underlying host location, host preference, and host-size selection of Pseudacteon phorid flies and highlights their direct and indirect effects on fire ant populations. Knowledge gained from parasitoid-ant interactions will enhance use of natural enemies as biological control agents for invasive social insects.

Molecular characterization of fire ants, Solenopsis spp., from Brazil based on analysis of mtDNA gene cytochrome oxidase I.

Species from the Solenopsis saevissima (Smith) (Hymenoptera: Formicidae) species group are native to South America and have a cosmopolitan distribution because they have been accidentally introduced in many countries around the world. In Brazil, they have a wide distribution, including urban areas. The present study was conducted to investigate the characterization of Solenopsis genus populations associated with urban/human interference sites in Brazil by analyzing the mitochondrial gene cytochrome oxidase I and estimating the degree of relatedness of these populations to make inferences about their phylogeny and also observe the patterns of mitochondrial haplotype (mitotype) distribution across their range. The results revealed complete geographical coherence and polyphyly for the Solenopsis invicta Buren and Solenopsis saevissima species groups, which confirms the diversity of the genera. It also suggests the possibility that reproductively-isolated populations occur, resulting in the evolutionary process of speciation. No predominant haplotype was found in the populations analyzed, but some were more prevalent.

Ants (Hymenoptera: Formicidae) in vineyards that are infested or uninfested with Eurhizococcus brasiliensis (Hemiptera: Margarodidae) in Southeastern Brazil.

The association between ants and mealybugs can result in damage to agriculture, including vineyards. In southern Brazil, the ant Linepithema micans F. contributes to the dispersal of Eurhizococcus brasiliensis (Wille) (ground pearl), a root mealybug that can lead to economic losses. In this study, the ant communities in vineyards that were infested or uninfested with ground pearls were evaluated in the primary municipalities that produce the Niágara Rosada variety of grapes in southeastern Brazil. The hypothesis of this study was that the composition of the ant community differs between vineyards with and without E. brasiliensis. The ants were collected using subterranean traps in 10 vineyards infested with this mealybug and 10 uninfested vineyards. There was no significant association between ground pearls and the composition or richness of the ant species. Solenopsis invicta (Buren) (Hymenoptera: Formicidae) was the most frequently observed, and Pheidole aberrans (Mayr), Pheidole subarmata (Mayr), and Brachymyrmex incisus F. were common, especially in the rainy season when ground-pearl nymphs were prevalent in the state of São Paulo. Species from preserved or specialized environments were recorded in the vineyards, even with the use of conventional management techniques.

Tetradonema solenopsis n. sp. (Nematoda: Tetradonematidae) Parasitic on the Red Imported Fire Ant Solenopsis invicta Buren from Brazil.

Explorations in Brazil to find parasites of the red imported fire ant, Solenopsis invicta Buren, have uncovered a new species of the Tetradonematidae, Tetradonema solenopsis n. sp. The nematode parasite was fatal to about 25% of the colony. The female nematodes are large and sausage shaped and the males are small and difficult to find, which is typical of the genus. The ant is a new host record for tetradonematids.