RESUMEN
The objective of this study was to evaluate the effect of aqueous extracts of Baccharis trimera, Plantago sp., Verbena officinalis and Arctium lappa on the M. javanica control in the greenhouse. The experiment was performed in plastic pots containing a soil mixture and sand 2:1 (volume: volume), it was infested with 4.000 eggs of Meloidogyne javanica and 200 mL of the respective aqueous extract was added. After seven days a tomato seedling was transplanted in each pot. The evaluations of the number of galls and eggs of the nematode by root system were performed after 50 days of transplanting the seedlings. The data was subjected to analysis of variance and the means were compared by Duncan test at 5% probability. None of the tested treatments reduced the number of galls and eggs of M. javanica per root system.
O objetivo desse ensaio foi avaliar o efeito de extratos aquosos de Baccharis trimera, Plantago sp., Verbena officinalis e Arctium lappa no controle de Meloidogyne javanica, em casa de vegetação. O experimento foi montado em vasos plásticos contendo uma mistura solo e areia 2:1 (volume : volume), infestada com 4.000 ovos de M. javanica e adicionado 200 mL dos respectivos extratos. Após sete dias foi transplantada uma muda de tomate em cada vaso. As avaliações do número de galhas e de ovos do nematoide por sistema radicular foram realizadas após 50 dias do transplantio das mudas. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Duncan, a 5 % de probabilidade. Nenhum dos tratamentos testados reduziu o número de galhas e de ovos de M. javanica por sistema radicular.
Asunto(s)
Baccharis , Tratamiento del Suelo/análisis , Tylenchoidea/parasitología , VerbenaRESUMEN
The objective of this study was to evaluate the effect of aqueous extracts of Baccharis trimera, Plantago sp., Verbena officinalis and Arctium lappa on the M. javanica control in the greenhouse. The experiment was performed in plastic pots containing a soil mixture and sand 2:1 (volume: volume), it was infested with 4.000 eggs of Meloidogyne javanica and 200 mL of the respective aqueous extract was added. After seven days a tomato seedling was transplanted in each pot. The evaluations of the number of galls and eggs of the nematode by root system were performed after 50 days of transplanting the seedlings. The data was subjected to analysis of variance and the means were compared by Duncan test at 5% probability. None of the tested treatments reduced the number of galls and eggs of M. javanica per root system.(AU)
O objetivo desse ensaio foi avaliar o efeito de extratos aquosos de Baccharis trimera, Plantago sp., Verbena officinalis e Arctium lappa no controle de Meloidogyne javanica, em casa de vegetação. O experimento foi montado em vasos plásticos contendo uma mistura solo e areia 2:1 (volume : volume), infestada com 4.000 ovos de M. javanica e adicionado 200 mL dos respectivos extratos. Após sete dias foi transplantada uma muda de tomate em cada vaso. As avaliações do número de galhas e de ovos do nematoide por sistema radicular foram realizadas após 50 dias do transplantio das mudas. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Duncan, a 5 % de probabilidade. Nenhum dos tratamentos testados reduziu o número de galhas e de ovos de M. javanica por sistema radicular.(AU)
Asunto(s)
Tylenchoidea/parasitología , Tratamiento del Suelo/análisis , Baccharis , VerbenaRESUMEN
Verbena officinalis L., Verbenaceae, commonly known as vervain, is a plant widely used in medicine. Despite of its widespread use in different traditional practices, the mechanisms of pharmacological actions of the plant and its volatile oil are still unclear. We evaluated the pro-apoptotic activity of V. officinalis essential oil and of its main component, citral, on lymphocytes collected from ten patients with chronic lymphocytic leukaemia (CLL), a disease in which a faulty apoptotic mechanism is still retained one of the primary pathogenic events, by adding to treated mononuclear cells, annexin-V, propidium iodide, and CD19. Apoptosis was also evaluated using anti-active-caspase-3 monoclonal antibody after permeabilization of the cells. Both V. officinalis essential oil and citral were found able to induce apoptosis in CLL cells and to activate caspase-3, which is considered the way by means they active apoptosis in B neoplastic cells. This data further support evidences that indicate natural compounds as possible lead structure to develop new therapeutic agents for CLL.