miRNA-based electrochemical biosensors for ovarian cancer.

Ovarian cancer, a prevalent and deadly cancer among women, presents a significant challenge for early detection due to its heterogeneous nature. MicroRNAs, short non-coding regulatory RNA fragments, play a role in various cellular processes. Aberrant expression of these microRNAs has been observed in the carcinogenesis-related processes of many cancer types. Numerous studies highlight the critical role of microRNAs in the initiation and progression of ovarian cancer. Given their clinical importance and predictive value, there has been considerable interest in developing simple, prompt, and sensitive miRNA biosensor strategies. Among these, electrochemical sensors have demonstrated advantageous characteristics such as simplicity, sensitivity, low cost, and scalability. These microRNA-based electrochemical biosensors are valuable tools for early detection and point-of-care applications. This article discusses the potential role of microRNAs in ovarian cancer and recent advances in the development of electrochemical biosensors for miRNA detection in ovarian cancer samples.

A novel fluorescent probe with high sensitivity and selectivity for "On-Off-On" sensors for Cu2+ cations and CN- anions.

A novel fluorescent probe NIPF was synthesized by the Suzuki reaction to recognize Cu2+ and CN-. With the addition of Cu2+, NIPF exhibited strong fluorescence quenching (90 % for NIPF) with a Ksv value of 3.4 × 106 M-1 and a detection limit of 9.04 × 10-10 M. Subsequently, CN- was added to the NIPF-Cu2+ solution, and [Cu(CN)x]n- was formed due to the strong interaction between Cu2+ and CN- leading to fluorescence recovery (89 % for NIPF-Cu2+). In addition, a detection limit of 3.6 × 10-8 M was obtained by fluorescence titration. Meanwhile, it was demonstrated that the sensor achieved 93 %-105 % recovery of Cu2+ in the tested environmental samples, and the practicability of Cu2+ and CN- detection were verified using hydrogels test, with significant color changes observed under 365 nm light. Accordingly, the fluorescent probe NIPF was used to recognize Cu2+ and CN- by the "on-off-on" sensors.

Warm red light emitting Eu(III) complexes synthesis and analysis including computational methods for photonic applications.

Six luminescent europium organic complexes have been synthesized and studied for their luminescent properties. The synthesized complexes were analyzed through elemental analysis, XRD, SEM, EDAX, FT-IR, NMR and thermogravimetry. The complexes exhibit crystalline behavior and possess decent thermal stability. Photoluminescence study on complexes were conducted in both solid and solution states, the results indicate the characteristic red emission. With the addition of ancillary ligands, water molecules are replaced from inner coordination sphere, leading to enhanced luminescence properties. The colorimetric parameters (CIE, CP%, CCT, u', v') suggest aptness of these complexes in red light illuminating OLEDs. The J-O parameters were calculated experimentally and theoretically with the help of LUMPAC software. Theoretical and experimental results agree well reflecting the efficacy of the outcomes. As a result of red emission, these complexes could have interesting photonics applications. The biological studies indicate the probable use of these complexes in the medical industry.

Atlantic salmon (Salmo salar) waste as a unique source of biofunctional protein hydrolysates: Emerging productions, promising applications, and challenges mitigation.

The Atlantic salmon is an extremely popular fish for its nutritional value and unique taste among several fish species. Researchers are focusing on the utilization of Atlantic salmon waste for generating protein hydrolysates rich in peptides and amino acids and investigating their health benefits. Several technological approaches, including enzymatic, chemical, and the recently developed subcritical water hydrolysis, are currently used for the production of Atlantic salmon waste protein hydrolysates. Hydrolyzing various wastes, e.g., heads, bones, skin, viscera, and trimmings, possessing antioxidant, blood pressure regulatory, antidiabetic, and anti-inflammatory properties, resulting in applications in human foods and nutraceuticals, animal farming, pharmaceuticals, cell culture, and cosmetics industries. Furthermore, future applications, constraints several challenges associated with industrial hydrolysate production, including sensory, safety, and economic constraints, which could be overcome by suggested techno processing measures. Further studies are recommended for developing large-scale, commercially viable production methods, focusing on eradicating sensory constraints and facilitating large-scale application.

Usabilidade de um aplicativo móvel de apoio aos profissionaisde saúde para cálculos de doses de vancomicina / Usability of a mobile application supporting healthprofessionals on vancomycin dosing calculation

O aplicativo móvel CalcVAN foi desenvolvido para auxiliar os profissionais de saúde para otimizar as doses de vancomicina em pacientes hospitalizados. Porém, é imprescindível avaliar a sua usabilidade antes de disponibilizá-lo para prática clínica. Assim, o objetivo do estudo é avaliar a usabilidade do aplicativo móvel na perspectiva dos profissionais de saúde. Trata-se de um estudo descritivo, de avaliação heurística da usabilidade de um aplicativo móvel. Foram convidados profissionais da área de saúde com expertise no tema de gerenciamento de antimicrobianos e vancomicina. O instrumento validado Smartphone Usability questionnaiRE (SURE) foi utilizado para mensuração da usabilidade por meio de um questionário on-line. Vinte e um especialistas participaram do estudo, com média de idade de 32,6 anos, sendo a maioria de mulheres (n = 14, 66,7%), profissionais farmacêuticos (n = 13, 61,9%), com pós-graduação lato sensu (n = 10, 47,6%), que trabalhavam em hospitais públicos ou privados (n = 15, 71,4%) e com média de experiência em 9,7 anos. Com base na interpretação dos resultados obtidos pelo instrumento SURE, a média de usabilidade geral do CalcVAN foi de 83 pontos, com escore menor de 78 e maior de 90 pontos. O teste de usabilidade foi enquadrado nos dois últimos níveis, 70 e 80, onde os profissionais de saúde passaram a concordar fortemente e totalmente, indicando que o aplicativo móvel apresenta uma usabilidade satisfatória. O CalcVAN atingiu uma usabilidade satisfatória e atende as necessidades e exigências dos profissionais de saúde, mostrando--se eficiente para realizar as funções propostas.

The CalcVAN app was developed to assist healthcare professionals in optimizing vancomycin doses for hospitalized patients. However, the usability test before making it available for clinical practice is essential. Therefore, the study aims to evaluate the usability of the app from the perspective of health professionals. A descriptive study, a heuristic evaluation of the usability of a mobile application was conducted. Healthcare professionals with expertise in antimicrobial management and vancomycin were invited to participate. The validated Smartphone Usability questionnaiRE (SURE) was used to measure usability through an online questionnaire. Twenty-one experts participated in the study, with a mean age of 32.6 years, mostly of them women (n = 14, 66.7%), pharmacists (n = 13, 61.9%), with postgraduate education (n = 10, 47.6%), working in private or public hospitals (n = 15, 71.4%), and a mean experience of 9.7 years. Overall usability score for CalcVAN was 83 points, ranging from a minimum of 78 to a maximum of 90 points. The usability test registered within the last two levels, 70 and 80, with users expressing strongly and fully agreed, indicating that the app demonstrates satisfactory usability. CalcVAN achieved satisfactory usability, fulfilling the needs and requirements of health professionals, proving to be efficient in performing the intended functions.

The WeThrive App and Its Impact on Adolescents Who Menstruate: Qualitative Study.

BACKGROUND: Heavy menstrual bleeding (HMB) affects up to 37% of adolescents. Without recognition, HMB can lead to other medical conditions resulting in diminished health-related quality of life. WeThrive, a new mobile health (mHealth) app, implements the pictorial bleeding assessment chart to identify HMB, and the adolescent Menstrual Bleeding Questionnaire to measure the effects of HMB on adolescents' health-related quality of life. If HMB is identified, WeThrive will connect users to local clinics for further assessment of their menstrual bleeding with a health care provider. OBJECTIVE: This study aimed to describe adolescents' experiences using WeThrive app. METHODS: This qualitative study was approved by the local Research Ethics Board in Halifax, Nova Scotia, and informed consent was provided by all participants. Individual semistructured interviews were held via videoconference with adolescents younger than 18 years, who had at least 1 menstrual period and had used WeThrive at least once. Interview transcripts were thematically analyzed by 2 investigators (MP and NMN) independently, and the κ statistic was calculated to determine the strength of correlation in themes. RESULTS: Five adolescents (mean age 15.5, range 13-18 years), participated in the interviews. All participants stated that WeThrive helps them better understand their menstrual periods by predicting period onset, recognizing menstrual symptoms, and identifying HMB. Four themes were identified: (1) the importance of visual features and usability, (2) newly obtained knowledge using WeThrive, (3) feature use depends on menstrual health, and (4) trustworthiness. There was substantial agreement on the identified themes (κ=0.73). CONCLUSIONS: WeThrive is visually appealing, and trustworthy, and helps users better understand their menstrual periods, including identifying HMB. By identifying HMB early, WeThrive has the potential to improve the recognition of bleeding disorders and iron deficiency in adolescents. WeThrive is a useful tool to help adolescents better understand their menstrual periods.

Mobile Apps for Vaccination Services: Content Analysis and Quality Assessment.

BACKGROUND: Vaccination services are increasingly in demand by the public, and mobile apps are an effective tool to meet that demand. However, the characteristics and quality of these apps are unknown. OBJECTIVE: Commonly used vaccination service apps on the market were surveyed with regard to quality, service content, and user experience to evaluate and guide users. METHODS: The Qimai Data mobile app data analytics platform was used to search for common vaccination service apps by keyword, and the WeChat and Alipay platforms were searched for apps. The apps included in the study were independently evaluated by two reviewers using the Mobile Application Rating Scale, and the service content and user experience of the apps were analyzed. The intragroup correlation coefficient between raters was used to measure interrater reliability. RESULTS: In the app stores of the four major Android platforms and the iOS app store, 1092 and 207 apps were found, respectively; 189 WeChat applets and 30 Alipay applets were also found. A total of 29 apps was ultimately included in this study according to the inclusion criteria, including 21 independent apps, 4 WeChat applets, and 4 Alipay applets. Significant differences were found between independent apps and applets in terms of the quality score (t449.57=-5.301; P<.001) and the subjective quality score (z=-4.753; P<.001). No significant differences were found between iOS and Android platforms in terms of the quality score (t1404=-2.55; P=.80) and the subjective quality score (z=-0.137; P=.89). There was good intragroup consistency among the raters. CONCLUSIONS: In this study, independent apps and nonindependent apps that rely on social and payment platforms for implementation were included in the vaccination services category. The overall quality of these apps was acceptable. Nonindependent running apps were found to have slightly lower scores and showed room for improvement, and scores for the participatory apps were found to be generally low overall.

The impact of a continuous care model utilizing a smartphone application on quality of life and anxiety levels among gynecologic cancer patients: a randomized controlled trial.

BACKGROUND: Patients diagnosed with gynecological cancers often face a range of complications that can impact their quality of life and increase their anxiety. Nursing models combined with mobile phone applications have the potential to improve outcomes for these patients. This study aimed to assess the impact of a continuous care model utilizing a smartphone application on quality of life and anxiety levels among gynecologic cancer patients. METHODS: This study involved two phases: (1) mobile App development and (2) implementation of the intervention. The two-group randomized controlled trial included 70 participants with gynecological cancers referred to medical centers affiliated with Shahrekord University of Medical Sciences in 2023. The participants were randomized into control or intervention groups (n = 35 per group). Finally, 68 patients completed the trial. The intervention group received an 8-week intervention incorporating the continuous care model, whereas the control group received routine care (the standard support provided by nurses both during and after hospitalization). The participants completed the Spielberger state-trait anxiety and quality of life (QLQ-C30) questionnaires before, immediately after, and two months after the intervention. The data were analyzed via the chi-square test, independent samples t test, analysis of covariance, and repeated-measures ANOVA. RESULTS: There were no significant differences in the baseline data between the two groups. However, after the intervention, the intervention group reported a significant increase in quality of life, with mean scores rising from 68.90 ± 17.50 to 73.78 ± 16.79 immediately after the intervention and to 80.61 ± 9.90 at the two-month follow-up. In contrast, the control group showed no significant improvement. Additionally, state anxiety significantly decreased in the intervention group from 51.64 ± 14.97 to 40.20 ± 11.70 at the follow-up, and trait anxiety scores in the intervention group decreased significantly from 49.91 ± 14.96 to 39.82 ± 10.28 at the follow-up, whereas the scores of the control group worsened. CONCLUSION: The intervention improved quality of life and reduced anxiety in patients with gynecological cancers. Given the scant attention given to mobile application-based follow-up in gynecologic cancer patients in previous studies, this approach can be incorporated into routine care to support patients, and it is recommended for nurses, health care providers, and physicians. TRIAL REGISTRATION: The study was registered as a randomized controlled trial in the Clinical Trial Registration Center of Iran. Registration Date: 2024-02-14, Registration Number: IRCT20231107059977N1.

Biogenic synthesis and characterization of antimicrobial, antioxidant, and antihemolytic zinc oxide nanoparticles from Desertifilum sp. TN-15 cell extract.

Cyanobacteria, being a prominent category of phototrophic organism, exhibit substantial potential as a valuable source of bioactive compounds and phytonutrients, including liposomes, amino derivatives, proteins, and carotenoids. In this investigation, a polyphasic approach was employed to isolate and characterize a newly discovered cyanobacterial strain from a rice field in the Garh Moor district of Jhang. Desertifilum sp. TN-15, a unique and less explored cyanobacterial strain, holds significant promise as a novel candidate for the synthesis of nanoparticles. This noticeable research gap underscores the novelty and untapped potential of Desertifilum sp. TN-15 in the field of nanomedicine. The characterization of the biogenically synthesized ZnO-NPs involved the application of diverse analytical techniques. Ultraviolet-visible spectroscopy revealed a surface plasmon resonance peak at 298 nm. Fourier transform infrared spectral analysis was utilized to confirm the involvement of biomolecules in the biogenic synthesis and stability. Scanning electron microscopy was employed to probe the surface morphology of the biogenic ZnO-NPs unveiling their size of 94.80 nm and star-shaped. Furthermore, X-ray diffraction analysis substantiated the crystalline nature of ZnO-NPs, with a crystalline size measuring 46 nm. To assess the physical stability of ZnO-NPs, zeta potential and dynamic light scattering measurements were conducted, yielding values of + 31.6 mV, and 94.80 nm, respectively, indicative of favorable stability. The antibacterial capabilities of Desertifilum sp. TN-15 are attributed to its abundance of bioactive components, including proteins, liposomes, amino derivatives, and carotenoids. Through the synthesis of zinc oxide nanoparticles (ZnO-NPs) with this strain, we have effectively used these chemicals to generate nanoparticles that exhibit noteworthy antibacterial activity against Staphylococcus aureus (MIC: 30.05 ± 0.003 µg/ml). Additionally, the ZnO-NPs displayed potent antifungal activity and antioxidant properties, as well as significant antihemolytic effects on red blood cells (IC50: 4.8 µg/ml). Cytotoxicity assessment using brine shrimps revealed an IC50 value of 3.1 µg/ml. The multifaceted actions of the biogenically synthesized ZnO-NPs underscore their potential applications in pharmacological and therapeutic fields. This study proposes a novel method for ZnO-NPs production utilizing the recently identified cyanobacterial strain Desertifilum sp. TN-15, highlighting the growing significance of biological systems in the environmentally friendly fabrication of metallic oxide nanomaterials.

A review of the botany, metabolites, pharmacology, toxicity, industrial applications, and processing of Polygalae Radix: the "key medicine for nourishing life".

Polygalae radix (PR) is the dried root of Polygala tenuifolia Willd. and Polygala sibirica L. and enjoys the reputation as the "key medicine for nourishing life." In this study, information about "Polygala tenuifolia Willd.," "Polygala sibirica L.," and "Yuanzhi" was retrieved from scientific databases, including Google Scholar, Baidu Scholar, Web of Science, PubMed, CNKI, and Wan Fang Data. Information from Chinese herbal medicine classics, Yaozhi Data, and the Gaide Chemical Network was also collected. Information related to botany, phytochemistry, pharmacology, toxicity, industrial applications, and processing is summarized in this paper to tap its potentialities and promote its further development and clinical application. More than 320 metabolites have been isolated from PR; saponins, xanthones, and oligosaccharide esters are the main functional metabolites. Pharmacological research shows that its pharmacological action mainly focuses on resisting nervous system diseases, and it also has the functions of anti-oxidation, anti-inflammation, anti-tumor, anti-pathogenic microorganisms and others. The gastrointestinal irritation of its saponins impeded its application, but this irritation can be reduced by controlling the dosage, compatibility with other herbs, or processing. The future progress of PR faces opportunities and challenges. More attention should be paid to the traditional application and processing methods of PR recorded in ancient books. The lack of safety and clinical studies has limited its application and transformation of achievements. Moreover, it is one-sided to take the content of only a few metabolites as the index of processing optimization and quality control, which cannot reflect the full pharmacological and toxicological activities of PR.

OpenAI o1-Preview vs. ChatGPT in Healthcare: A New Frontier in Medical AI Reasoning.

This editorial explores the recent advancements in generative artificial intelligence with the newly-released OpenAI o1-Preview, comparing its capabilities to the traditional ChatGPT (GPT-4) model, particularly in the context of healthcare. While ChatGPT has shown many applications for general medical advice and patient interactions, OpenAI o1-Preview introduces new features with advanced reasoning skills using a chain of thought processes that could enable users to tackle more complex medical queries such as genetic disease discovery, multi-system or complex disease care, and medical research support. The article explores some of the new model's potential and other aspects that may affect its usage, like slower response times due to its extensive reasoning approach yet highlights its potential for reducing hallucinations and offering more accurate outputs for complex medical problems. Ethical challenges, data diversity, access equity, and transparency are also discussed, identifying key areas for future research, including optimizing the use of both models in tandem for healthcare applications. The editorial concludes by advocating for collaborative exploration of all large language models (LLMs), including the novel OpenAI o1-Preview, to fully utilize their transformative potential in medicine and healthcare delivery. This model, with its advanced reasoning capabilities, presents an opportunity to empower healthcare professionals, policymakers, and computer scientists to work together in transforming patient care, accelerating medical research, and enhancing healthcare outcomes. By optimizing the use of several LLM models in tandem, healthcare systems may enhance efficiency and precision, as well as mitigate previous LLM challenges, such as ethical concerns, access disparities, and technical limitations, steering to a new era of artificial intelligence (AI)-driven healthcare.

An examination of auxetic componentry for applications in human-centred biomedical product design settings.

This paper explores how the examination of additively manufactured auxetic componentry can be applied in human-centred design settings with particular focus on biomedical products. Firstly, the design applications of auxetics are detailed followed by a review of the key problems facing practical researchers in the field with the treatment of boundary conditions identified as a key issue. The testing setup that is then introduced utilises a novel method of part mounting and facilitates optical analysis and real-time force-displacement measurements. A study is advanced that analyses three different auxetic structures (re-entrant, chiral, and semi-rigid), a set of samples of which were additively manufactured in flexible TPU material. A range of parameters were varied across the three designs including interior geometry and wall thicknesses in order to demonstrate the effectiveness of the setup for the examination of the different structures. The results from these examinations are subsequently discussed and a number of suggestions made regarding how this kind of analysis may be integrated into novel design development workflows for achieving human-centred biomedical devices which often require detailed consideration of ergonomic and usability factors.

Additively Manufactured Zirconia for Dental Applications with In Situ Color Gradation Control.

The current process for creating zirconia reconstructions for teeth is time-consuming, expensive, and results in tool wear and raw material waste. An alternative method, near-net shape additive manufacturing capable of producing samples with color gradient is presented as an innovative and more efficient solution. A low-cost robocasting system, which enabled the co-extrusion of two different ceramic inks, was designed and developed. Moreover, hydrogel-based ceramic inks, with rheological properties suitable for the present system, containing commercially available Yttria-stabilized zirconia (white and yellow) powders, were produced. Parts of different color shades and color gradients, with a high aspect ratio and good green body stability, were printed. In addition, precise color measurements were carried out, and co-extruded parts were compared with ultraviolet-C photofunctionalized parts. High fractions of binder in green bodies caused distortions in the samples during air drying and upon sintering. Debinding and sintering at 1500°C yielded parts of relatively low density (4.90-5.09 g/cm3) and hardness (500 HV10-1100 HV10). A slightly different sintering behavior was observed for parts of different compositions. Density and hardness increased with the fraction of iron oxide-containing ink.

Recent innovations in 3D solar evaporators and their functionalities.

Interfacial solar evaporation (ISE) has emerged as a promising technology to alleviate global water scarcity via energy-efficient purification of both wastewater and seawater. While ISE was originally identified and developed during studies of simple double-layered two-dimensional (2D) evaporators, observed limitations in evaporation rate and functionality soon led to the development of three-dimensional (3D) evaporators, which is now recognized as one of the most pivotal milestones in the research field. 3D evaporators significantly enhance the evaporation rates beyond the theoretical limits of 2D evaporators. Furthermore, 3D evaporators could have multifaceted functionalities originating from various functional evaporation surfaces and 3D structures. This review summarizes recent advances in 3D evaporators, focusing on rational design, fabrication and energy nexus of 3D evaporators, and the derivative functions for improving solar evaporation performance and exploring novel applications. Future research prospects are also proposed based on the in-depth understanding of the fundamental aspects of 3D evaporators and the requirements for practical applications.

Biomaterials for reliable wearable health monitoring: Applications in skin and eye integration.

Recent advancements in biomaterials have significantly impacted wearable health monitoring, creating opportunities for personalized and non-invasive health assessments. These developments address the growing demand for customized healthcare solutions. Durability is a critical factor for biomaterials in wearable applications, as they must withstand diverse wearing conditions effectively. Therefore, there is a heightened focus on developing biomaterials that maintain robust and stable functionalities, essential for advancing wearable sensing technologies. This review examines the biomaterials used in wearable sensors, specifically those interfaced with human skin and eyes, highlighting essential strategies for achieving long-lasting and stable performance. We specifically discuss three main categories of biomaterials-hydrogels, fibers, and hybrid materials-each offering distinct properties ideal for use in durable wearable health monitoring systems. Moreover, we delve into the latest advancements in biomaterial-based sensors, which hold the potential to facilitate early disease detection, preventative interventions, and tailored healthcare approaches. We also address ongoing challenges and suggest future directions for research on material-based wearable sensors to encourage continuous innovation in this dynamic field.

How can physicians adopt AI-based applications in the United Arab Emirates to improve patient outcomes?

Objective: The enabling and derailing factors for using artificial intelligence (AI)-based applications to improve patient care in the United Arab Emirates (UAE) from the physicians' perspective are investigated. Factors to accelerate the adoption of AI-based applications in the UAE are identified to aid implementation. Methods: A qualitative, inductive research methodology was employed, utilizing semi-structured interviews with 12 physicians practicing in the UAE. The collected data were analyzed using NVIVO software and grounded theory was used for thematic analysis. Results: This study identified factors specific to the deployment of AI to transform patient care in the UAE. First, physicians must control the applications and be fully trained and engaged in the testing phase. Second, healthcare systems need to be connected, and the AI outcomes need to be easily interpretable by physicians. Third, the reimbursement for AI-based applications should be settled by insurance or the government. Fourth, patients should be aware of and accept the technology before physicians use it to avoid negative consequences for the doctor-patient relationship. Conclusions: This research was conducted with practicing physicians in the UAE to determine their understanding of enabling and derailing factors for improving patient care through AI-based applications. The importance of involving physicians as the accountable agents for AI tools is highlighted. Public awareness regarding AI in healthcare should be improved to drive public acceptance.

Preparations, Applications, Patents, and Marketed Formulations of Nanoemulsions - A Comprehensive Review.

Nanoemulsions have emerged as versatile colloidal dispersions with promising applications in various fields, including pharmaceuticals, food, and cosmetics. These nano-sized emulsions, stabilized by surfactants, offer unique advantages such as enhanced ingredient penetration efficacy and versatile dosage forms. This article provides an extensive overview of nanoemulsions, covering their composition, methods of preparation, and applications in drug delivery, the food industry, and cosmetics. Various high-energy and low-energy methods for nanoemulsion preparation are discussed, along with their advantages and limitations. Additionally, the article highlights the potential of nanoemulsions in improving drug bioavailability, stability, and therapeutic efficacy, especially in oral, topical, parenteral, intranasal, ocular, and pulmonary drug delivery. Furthermore, nanoemulsions are explored as carriers for encapsulating flavoring agents, nutraceuticals, and natural preservatives in the food industry, as well as their use in cosmetic formulations. Current clinical trials involving nanoemulsions and recent patents in the field are also summarized, providing insights into ongoing research and development efforts. Lastly, a selection of marketed nanoemulsion formulations is presented, showcasing their practical applications and commercial availability. Overall, nanoemulsions hold great promise as effective delivery systems with broad applications across various industries.

Recent advancement of novel marine fungi derived secondary metabolite fibrinolytic compound FGFC in biomedical applications: a review.

For several decades, products derived from marine natural sources (PMN) have been widely identified for several therapeutic applications due to their rich sources of bioactive sub-stances, unique chemical diversity, biocompatibility and excellent biological activity. For the past 15 years, our research team explored several PMNs, especially fungi fibrinolytic compounds (FGFCs). FGFC is an isoindolone alkaloid derived from marine fungi, also known as staplabin analogs or Stachybotrys microspora triprenyl phenol (SMTP). For instance, our previous studies explored different types of FGFCs such as FGFC 1, 2, 3 and 4 from the marine fungi Stachybotrys longispora FG216 derived metabolites. The derivatives of FGFC are potentially employed in several disease treatments, mainly for stroke, cancer, ischemia, acute kidney injury, inflammation, cerebral infarction, thrombolysis and hemorrhagic activities, etc. Due to the increasing use of FGFCs in pharmaceutical and biomedical applications, it is important to understand the fundamental signaling concept of FGFCs. Hence, for the first time, this review collectively summarizes the background, types, mode of action and biological applications of FGFCs and their current endeavors for future therapies.

Laser-Induced Breakdown Spectroscopy as an Accurate Forensic Tool for Bone Classification and Individual Reassignment.

This article provides a detailed discussion of the evidence available to date on the application of laser-induced breakdown spectroscopy (LIBS) and supervised classification methods for the individual reassignment of commingled bone remains. Specialized bone chemistry studies have demonstrated the suitability of bone elemental composition as a distinct individual identifier. Given the widely documented ability of the LIBS technique to provide elemental emission spectra that are considered elemental fingerprints of the samples analyzed, the analytical potential of this technique has been assessed for the investigation of the contexts of commingled bone remains for their individual reassignment. The LIBS bone analysis consists of the direct ablation of micrometric portions of bone samples, either on their surface or within their internal structure. To produce reliable, accurate, and robust bone classifications, however, the available evidence suggests that LIBS spectral information must be processed by appropriate methods. When comparing the performance of seven different supervised classification methods using spectrochemical LIBS data for individual reassociation, those employing artificial intelligence-based algorithms produce analytically conclusive results, concretely individual reassociations with 100% accuracy, sensitivity, and robustness. Compared to LIBS, other techniques used for the purpose of interest exhibit limited performance in terms of robustness, sensitivity, and accuracy, as well as variations in these results depending on the type of bones used in the classification. The available literature supports the suitability of the LIBS technique for reliable individual reassociation of bone remains in a fast, simple, and cost-effective manner without the need for complicated sample processing.

Advances in stimuli-responsive gold nanorods for drug-delivery and targeted therapy systems.

In recent years, the use of gold nanorods (AuNRs) has garnered considerable attention in biomedical applications due to their unique optical and physicochemical properties. They have been considered as potential tools for the advanced treatment of diseases by various stimuli such as magnetic fields, pH, temperature and light in the fields of targeted therapy, imaging and drug delivery. Their biocompatibility and tunable plasmonic properties make them a versatile platform for a range of biomedical applications. While endogenous stimuli have limited cargo delivery control at specific sites, exogenous stimuli are a more favored approach despite their circumscribed penetration depth for releasing the cargo at the specific target. Dual/multi-stimuli responsive AuNTs can be triggered by multiple stimuli for enhanced control and specificity in biomedical applications. This review provides to provide a summary of the biomedical applications of stimuli-responsive AuNRs, including their endogenous and exogenous properties, as well as their dual/multi-functionality and potential for clinical delivery. This review provides a comprehensive review on the improvement of therapeutic efficacy and the effective control of drug release with AuNRs, highlights AuNRs design strategies in recent years, discusses the advantages or challenges so far in the field of AuNRs. Finally, we have addressed the clinical translation bio-integrated nanoassemblies (CTBNs) in this field.