Impact of different fermentation times on the microbiological, chemical, and sensorial profile of coffees processed by self-induced anaerobiosis fermentation.

Variation in fermentation time may be an essential alternative to provide coffee beverages with different and unique sensory profiles. This work investigated the microbiological, chemical, and sensory changes in coffees submitted to different fermentation durations (0, 24, 48, 72, and 96 h). Self-induced anaerobiosis fermentation (SIAF) was used, and two treatments were performed: spontaneous fermentation and inoculation with S. cerevisiae CCMA0543. Microbiological analyses were performed, and the permanence of the inoculum was monitored. Chromatography (sugars, organic acids, and volatile compounds) was analyzed, and sensory analysis (temporal dominance of sensations - TDS) was performed. A total of 228 isolates were identified during spontaneous fermentation. The dominant bacteria and yeasts were Leuconostoc mesenteroides, Lactiplantibacillus plantarum, Staphylococcus warneri, Bacillus sp., Torulaspora delbrueckii, Hanseniaspora uvarum, and Meyerozyma caribbica. High concentrations of citric (18.67 mg.g- 1) and succinic (5.04 mg.g- 1) acids were detected at 96 h in SIAF fermentation. One hundred twenty-one volatile compounds were detected, but 22 were detected only in inoculated coffees. In spontaneous fermentation, 48 h of fermentation showed woody notes, while 72 h showed chestnuts. However, in the inoculated coffee, 72 h of fermentation showed high fruity dominance, and 96 h of fermentation was the only one with herbaceous notes. In addition, yeast inoculation increased the intensity of caramel notes in the first 48 h and increased the fruity flavor after 72 h of fermentation. Therefore, the type of fermentation (with or without inoculation) and the chosen fermentation time will depend on the sensorial profile the producer intends to obtain.

Optimizing cellulose fraction for enhanced utility: Comparative pre-treatment of Agave tequilana Weber var. blue bagasse fiber for sustainable applications.

In recent decades, natural fibers have emerged as an alternative to synthetic fibers due to their renewable nature, lower environmental impact, and comparable strength properties. Agave bagasse, a byproduct of agave juice extraction in Mexico, stands out for its potential in various industrial applications, notably biocomposite production. Bagasse is rich in cellulose, along with hemicellulose and lignin. Cellulose is the most suitable to be converted into valuable products, and it is versatile, renewable, and biodegradable. An effective pre-treatment is crucial to enrich its fraction. This study aims to determine the optimal pre-treatment conditions for the agave bagasse. Three different pre-treatments were tested, acid (H2SO4), enzymatic (Cellic® HTec2 enzymatic preparation), and sequence of acid-enzymatic (sulfuric acid and Cellic® HTec2), to determine which pre-treatment got the optimal cellulose fraction increase. The acid pre-treatment was conducted over three time ranges (5, 10, and 15 min) at different acid concentrations (1%, 1.5%, and 2%). Enzymatic reactions were conducted over 24 h, testing three different enzyme concentrations (1.5%, 3%, 4.5%). The sequential pre-treatment utilized the optimal conditions derived from the acid experiments (1.5% H2SO4 for 10 min), followed by enzymatic reactions carried out over three different durations (6, 12, and 24 h). The findings revealed that a 1.5% acid concentration applied for 10 min was the most efficient pre-treatment method. This pre-treatment resulted in a 1.9-fold increase in the cellulose fraction while reducing hemicellulose content by 30%. The hemicellulose reduction was confirmed through Fourier Transform IR spectroscopy (FTIR) analysis, complemented by scanning electron microscopy (SEM) observations highlighting physical alterations in the fiber structure. Furthermore, thermogravimetric analysis (TGA) demonstrated improved thermal stability, suggesting potential use in biocomposites. Future research should evaluate the environmental impact of optimized pre-treatment methods for agave bagasse.

Probabilistic Risk Assessment of Metals, Acrylamide and Ochratoxin A in Instant Coffee from Brazil, Colombia, Mexico and Peru.

This study analysed the probabilistic risk to consumers associated with the presence of iAs, Cd, Cr, Hg, Pb, acrylamide (AA) and ochratoxin A (OTA) in instant coffee from Brazil, Colombia, Mexico and Peru. The results found iAs to be the metal with the highest concentrations (3.50 × 10-2 to 6.00 × 10-2 mg/kg), closely followed by Pb (1.70 × 10-2 to 2.70 × 10-2 mg/kg) and Cr (5.00 × 10-3 to 1.00 × 10-2 mg/kg), although these differences were not significant between countries. Cd and Hg were not detected. Focusing on AA, the concentrations ranged from 1.77 × 10-1 mg/kg (Peru) to 4.77 × 10-1 mg/kg (Brazil), while OTA ranged from 1.32 × 10-3 (Peru) to 1.77 × 10-3 mg/kg (Brazil) with significant differences between countries in both cases. As regards risk, the hazard quotient and hazard index were less than 1, meaning that the consumption of instant coffee represents a low level of concern for non-genotoxic effects. The results of the combination of margin of exposure and probability of exceedance indicated that the non-genotoxic effects of Pb, AA and OTA pose no threat. However, the probability values of suffering cancer from iAs and AA (between 1 × 10-6 and 1 × 10-4) indicated a moderate risk and that management measures should be taken.

Rearing and 60Co radiation do not affect attractiveness but alter the volatile profiles released by Anastrepha obliqua calling males.

Calling males of Anastrepha obliqua release volatile compounds to attract conspecific males to form leks and females to mate. Male volatiles from Mexican and Brazilian populations of A. obliqua have been previously identified. However, there are differences in the number and identity of volatile compounds between the populations. These differences in volatile profiles may be due to male origin (e.g. wild or mass-reared flies) or methodological issues (e.g. sampling techniques). In this study, we evaluated the attractiveness of wild, laboratory non-irradiated, and laboratory-irradiated flies under semi-field conditions. Male volatiles were collected using dynamic headspace sampling (DHS) and solid-phase microextraction (SPME) techniques, and identified using gas chromatography-coupled mass spectrometry. The results showed no difference in the attractiveness of wild, laboratory non-irradiated, and irradiated males to females. However, the number of captured females differed according to the origin; wild and non-irradiated females were captured more frequently than the irradiated flies. A total of 21 compounds were found using SPME, whereas only 12 were collected using DHS, although the relative amounts of these compounds were higher than those obtained using the former sampling technique. In addition, only laboratory non-irradiated males released α-pinene and menthol, which have not been previously reported in this fruit fly species. Additionally, we identified novel compounds in A. obliqua; however, certain compounds previously reported were not detected. This study suggests that despite the qualitative and quantitative variations in the volatile profiles of A. obliqua males, their attractiveness was unaffected.

Radiotherapy in the head and neck region influences the chemical and mechanical properties of intraradicular dentin.

OBJECTIVES: To investigate the chemical and mechanical properties of intraradicular dentin submitted to radiotherapy. MATERIALS AND METHODS: Sixteen mandibular incisors were divided into two groups (n = 8): non-irradiated and irradiated. The irradiated teeth were obtained from head and neck radiotherapy patients, with a total dose ranging from 70.2 to 72 Gy divided into 1.8 Gy daily. After sample preparation, intraradicular dentin slices of each root third were evaluated by Raman spectroscopy, energy dispersive spectroscopy and Knoop microhardness test. Data were analyzed by Two-way ANOVA and Tukey's test (α = 0.05). RESULTS: In Raman spectroscopy, carbonate and amide III showed a significant difference for irradiation and third (carbonate p = 0.021 and p < 0.001; amide III p < 0.001 and p = 0.001, respectively). For amide I, there was a significant difference for third (p < 0.001). For carbonate/mineral ratio, there was a significant difference for irradiation (p = 0.0016) and third (p < 0.001), with the irradiated middle third showing the lowest values. For amide I/amide III ratio, there was a significant difference for irradiation (p = 0.005) in the cervical third. In energy dispersive spectroscopy, carbon (p = 0.004; p = 0.020), phosphorus (p < 0.001; p = 0.009) and calcium (p = 0.008; p = 0.007) showed differences for irradiation and third, with the irradiated groups presenting lower values in cervical and middle thirds. For calcium/phosphorus ratio, there was a significant difference for irradiation (p < 0.001) in cervical and middle thirds. Regarding microhardness, there was a significant difference for irradiation (p < 0.001), with all irradiated groups showing lower microhardness values. CONCLUSIONS: The radiotherapy altered the chemical and mechanical properties of intraradicular dentin, mainly in the cervical and middle root thirds.

The antimicrobial effect and mechanism of the Artemisia argyi essential oil against bacteria and fungus.

Artemisia argyi is a traditional Chinese herb with antibacterial, antifungal, and antitumor activities. The essential oil of Artemisia argyi was extracted using the steam distillation method in this study. The chemical composition of the essential oil was analyzed using the gas chromatography-mass spectrometry method. Agar disc diffusion and double-broth dilution assays were used to detect the antimicrobial activity of the essential oil. Subsequently, the antimicrobial mechanisms were explored through cytomembrane permeability assay and electron microscopy. Based on gas chromatography-mass spectrometry analysis, 25 compounds were detected, including 13.76% cineole, 6.77% terpinen-4-ol, 6.68% 3-dione, 1,7,7-trimethyl-, 4.07% 3-cyclohexen-1-ol, 4-methyl-1-(1-methylethyl)-acetate, 3.58% 1-isopropyl-2-methylbenzene, and 1.58% g-terpinene. The essential oil was tested for antimicrobial activity, and the IC50 values for Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Listeria monocytogenes, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Candida albicans were determined to be 25.51 ± 2.29, 49.53 ± 0.86, 52.40 ± 1.49, 52.76 ± 1.60, 73.99 ± 1.38, 65.52 ± 0.95, and 214.98 ± 3.27 µg mL-1, respectively. For essential oil interaction with cytoderm, the microorganisms treated by 1 × IC50 and 2 × IC50 concentration of essential oil both represented positive test results. Additionally, the alkaline phosphatase levels showed a direct correlation with concentration and treatment duration (range from 0 to 8 h). The interaction between essential oils and the cytomembrane was investigated by examining samples containing one of three test strains (Staphylococcus aureus, Escherichia coli, and Candida albicans), essential oil, and voltage-sensitive fluorescent dye disc35. The results demonstrated a significant increase in fluorescence levels within the solution upon introduction of the essential oil-treated strains. The findings of our research suggest that the essential oil disrupts the cytoderm and cytomembrane, thereby exhibiting antimicrobial activity.

In vitro diffusion of plant phenolics through the skin: A review update.

OBJECTIVE: Excessive skin exposure to deleterious environmental variables results in inflammation as well as molecular and cellular impairments that compromise its functionality, aesthetic qualities, and overall well-being. The implementation of topical administration of antioxidants and other compounds as a method for preventing or reversing damage is a rational approach. Numerous phenolic compounds derived from plants have demonstrated capabilities such as scavenging free radicals and promoting tissue healing. However, the primary obstacle lies in effectively delivering these compounds to the specific place on the skin, and accurately forecasting their diffusion through the skin can assist in determining the most effective tactics. Hence, this article provides a comprehensive analysis of recent literature pertaining to the in vitro skin diffusion characteristics of plant phenolics. The aim is to gain a deeper understanding of their behaviour when present in various forms such as solutions, suspensions, and formulations. METHOD: The data on plant extracts and isolated plant phenolic compounds in vitro skin diffusion assays published over the last six years were compiled and discussed. RESULTS: Even though the gold standard Franz diffusion cell is the most commonly used in the assessment of in vitro plant phenolic skin diffusion profiles, a plethora of skin models and assay conditions are reported for a variety of compounds and extracts in different vehicles. CONCLUSION: The presence of numerous models and vehicles poses a challenge in creating correlations among the existing data on plant phenolic compounds. However, it is possible to draw some general conclusions regarding molecular, vehicle, and skin characteristics based on the gathered information.

OBJECTIF: Une exposition excessive de la peau a des variables environnementales délétères entraîne une inflammation ainsi que des déficiences moléculaires et cellulaires qui compromettent sa fonctionnalité, ses qualités esthétiques et son bien­être général. La mise en œuvre de l'administration topique d'antioxydants et d'autres composés comme méthode de prévention ou d'inversion des dommages est une approche rationnelle. De nombreux composés phénoliques dérivés de plantes ont démontré des capacités telles que l'élimination des radicaux libres et la promotion de la cicatrisation des tissus. Cependant, le principal obstacle réside dans l'administration efficace de ces composés à un endroit spécifique de la peau, et une prévision précise de leur diffusion a travers la peau peut aider à déterminer les tactiques les plus efficaces. Par conséquent, cet article fournit une analyse complète de la littérature récente concernant les caractéristiques de diffusion cutanée in vitro des composés phénoliques végétaux. L'objectif est de mieux comprendre leur comportement lorsqu'ils sont présents sous diverses formes telles que solutions, suspensions et formulations. MÉTHODES: Les données sur les extraits de plantes et les composés phénoliques végétaux isolés in vitro par diffusion cutanée, publiées au cours des six dernières années, ont été compilées et discutées. RÉSULTATS: Même si la cellule de diffusion Franz de référence est la plus couramment utilisée dans l'évaluation des profils de diffusion cutanée phénoliques végétales in vitro, une pléthore de modelés de peau et de conditions d'analyse sont rapportées pour une variété de composés et d'extraits dans différents véhicules. CONCLUSION: La présence de nombreux modèles et véhicules pose un défi dans la création de corrélations entre les données existantes sur les composés phénoliques végétaux. Cependant, il est possible de tirer des conclusions générales concernant les caractéristiques moléculaires du véhicule et de la peau sur la base des informations recueillies.

Changes in flax yield and quality in response to various mineral nutrition / Alterações no rendimento e qualidade do linho em resposta a vários nutrientes minerais

The researchers of Russian State Agrarian University, Moscow Timiryazev Agricultural Academy in 2013-2016 conducted a long-term stationary experiment to study chemical and toxicological properties of fiber flax, Voskhod variety, growing on sod-podzolic soil in the soil and climate of the Moscow region. Test plots were selected with following crop rotation options: without fertilizers, without liming; without fertilizers, with liming; N100P150K120 (kg a.i./ha), without liming; N100P150K120, with liming; N100P150K120 + manure 20 t/ha, without liming; N100P150K120 + manure 20 t/ha, with liming. The agro-climatic conditions of the growing seasons during the research years did not have a negative impact on the growth and development of fiber flax, the hydro-thermal index was 1.1 in 2013, -1.05 in 2014, 1.5 in 2015, and 1.5 in 2016. The maintained crop rotation and the introduction of a full range of mineral and organic fertilizers has been found to contribute to high yields of flax in terms of fiber (18.5-18.9 hwt/ha) and seeds (7.9-8.3 hwt/ha). The seeds contain 16.9-19.5% protein and 33.5-39.4% lipids. The yield of flaxseed oil from seeds ranged from 19.5-35.7% on average for different variants of the experiment. The peroxide number index was 2.5-1.5 mg-eq O2/kg, the acid number index was 1.1-1.9 mg KOH/g, which corresponds to obtaining high-quality linseed oil in compliance with quality standards for all variants of the experiment.

Os pesquisadores da Universidade Agrária Estatal Russa, Academia Agrícola Timiryazev de Moscou, de 2013 a 2016, realizaram um experimento estacionário de longo prazo para estudar as propriedades químicas e toxicológicas da fibra de linho, variedade Voskhod, crescendo em solo sod-podzólico e no clima da região de Moscou. As parcelas-teste foram selecionadas com as seguintes opções de rotação de culturas: sem fertilizantes e sem calagem; sem fertilizantes e com calagem; N100P150K120 (kg ia/ha), sem calagem; N100P150K120 e com calagem; N100P150K120 + esterco 20 t/ha e sem calagem; N100P150K120 + esterco 20 t/há e com calagem. As condições agroclimáticas das épocas de cultivo durante os anos de pesquisa não tiveram impacto negativo no crescimento e desenvolvimento do linho têxtil, o índice hidrotérmico foi de 1,1 em 2013, -1,05 em 2014, 1,5 em 2015 e 1,5 em 2016. Verificou-se que a manutenção da rotação de culturas e a introdução de uma gama completa de fertilizantes minerais e orgânicos contribuem para altos rendimentos de linho em termos de fibra (18,5-18,9 hwt/ha) e sementes (7,9-8,3 hwt/ha). As sementes contêm 16,9-19,5% de proteína e 33,5-39,4% de lipídios. O rendimento de óleo de linhaça das sementes variou de 19,5 a 35,7% em média para diferentes variantes do experimento. O índice de peróxido foi de 2,5-1,5 mg-eq O2/kg, o índice de acidez foi de 1,1-1,9 mg KOH/g, o que corresponde à obtenção de óleo de linhaça de alta qualidade em conformidade com os padrões de qualidade para todas as variantes do experimento.

Dual-energy X-ray absorptiometry: an effective approach for predicting broiler chicken body composition.

Two trials were carried out to develop and validate linear regression equations for body composition prediction using Dual-energy X-ray absorptiometry (DEXA). In Trial 1, 300 Cobb500 male chickens raised from 1 to 42 d of age were scanned in DEXA to estimate total weight, fat mass, soft lean tissue (SLT) mass, bone mineral content (BMC), and fat percentage. DEXA estimates were compared to body ash, crude fat, SLT (sum of protein and water) and scale body weight. The dataset was split, with 70% used for prediction equations development and 30% for testing, and the 5k-fold cross-validation analysis was used to optimize the equations. The R2, mean absolute error (MAE), and root-mean-squared error (RMSE) were used as precision and accuracy indicators. A negative correlation (ρ = -0.27) was observed for ash content, while no correlation was observed for protein content (P > 0.05). Predictive linear equations were developed to assess broiler weight (R2 = 0.999, MAE = 25.12, RMSE = 38.99), fat mass (R2 = 0.981, MAE = 13.87, RMSE = 21.28), ash mass (R2 = 0.956, MAE = 3.98, RMSE = 5.61), SLT mass (R2 = 0.997, MAE = 35.73, RMSE = 52.45), water mass (R2 = 0.997, MAE = 29.56, RMSE = 43.94), protein mass (R2 = 0.989, MAE = 12.94, RMSE = 19.05), fat content (R2 = 0.855, MAE = 0.81, RMSE = 1.05), SLT content (R2 = 0.658, MAE = 1.01, RMSE = 1.28), and water content (R2 = 0.678, MAE = 0.99, RMSE = 1.27). All equations passed the test. In Trial 2, 395 Cobb500 male chickens were raised from 1 to 42 d of age and used for validation of prediction equations. The equations developed for weight, fat mass, ash mass, SLT mass, water mass, and protein mass were validated. In conclusion, DEXA was found to be an effective approach for measuring the body composition of broilers when using predictive equations validated in this study for estimate calibration.

Differentiating True and False Cinnamon: Exploring Multiple Approaches for Discrimination.

This study presents a comprehensive literature review that investigates the distinctions between true and false cinnamon. Given the intricate compositions of essential oils (EOs), various discrimination approaches were explored to ensure quality, safety, and authenticity, thereby establishing consumer confidence. Through the utilization of physical-chemical and instrumental analyses, the purity of EOs was evaluated via qualitative and quantitative assessments, enabling the identification of constituents or compounds within the oils. Consequently, a diverse array of techniques has been documented, encompassing organoleptic, physical, chemical, and instrumental methodologies, such as spectroscopic and chromatographic methods. Electronic noses (e-noses) exhibit significant potential for identifying cinnamon adulteration, presenting a rapid, non-destructive, and cost-effective approach. Leveraging their capability to detect and analyze volatile organic compound (VOC) profiles, e-noses can contribute to ensuring authenticity and quality in the food and fragrance industries. Continued research and development efforts in this domain will assuredly augment the capacities of this promising avenue, which is the utilization of Artificial Intelligence (AI) and Machine Learning (ML) algorithms in conjunction with spectroscopic data to combat cinnamon adulteration.

Impact of Drying Process on the Phenolic Profile and Antioxidant Capacity of Raw and Boiled Leaves and Inflorescences of Chenopodium berlandieri ssp. berlandieri.

C. berlandieri ssp. berlandieri (C. berlandieri) is one of the most common members of the group of plants known as quelites, which are dark leafy greens widely consumed in Mexico. This study aimed to evaluate the impact of two drying procedures (oven drying and freeze-drying/lyophilization) on the polyphenolic composition, antioxidant capacity, and proximal chemical analysis of C. berlandieri leaves and inflorescences (raw or boiled). The results indicated that the raw freeze-dried samples had higher amounts (p < 0.05) of total phenolic compounds, total flavonoids, and antioxidant capacity, mainly in the inflorescence. The oven-dried samples showed an increased concentration of polyphenols after boiling, while the lyophilized samples showed a slightly decreased concentration. The drying process was observed to have little impact on the proximal chemical composition. Quantification by UPLC-DAD-ESI-QToF/MS identified up to 23 individual phenolic compounds, with freeze-dried samples showing higher amounts of individual compounds compared with oven-dried. Procyanidin B2 was found exclusively in the inflorescences. The inflorescences have a higher content of phenolic compounds and greater antioxidant capacity than the leaves. Regardless of the drying process, the leaves and inflorescences of C. berlandieri contain an interesting variety of phenolic compounds that may have beneficial effects on health.

Aprovechamiento alternativo de calamar (Dosidicus gigas) como fuente de proteína para la fortificación de pan tipo baguette / Alternative use of squid (Dosidicus gigas) flour as a protein source to improve the nutritional quality of baguette bread

Introducción. Los cereales tienen aminoácidos limitantes y mezclarlos con harinas de origen marino presentan un mejor equilibrio en éstos mejorando la composición nutricional en productos de panificación, principalmente de proteína y aminoácidos. Objetivo. Desarrollar panes tipo "baguette" sustituyendo parcialente harina de trigo (HT) por harina de calamar (HC) y evaluar su calidad física, química y sensorial. Materiales y métodos. Se utilizó la técnica de masa esponja incorporando 7,5, 10 y 12,5 % de HC. Análisis físicos: tiempo de amasado, peso y fermentación de la masa, volumen específico, peso de los panes (g), % pérdida humedad y color de miga; análisis químicos: humedad, proteína cruda, cenizas, extracto etéreo, fibra cruda, carbohidratos, aminoácidos indispensables y energía bruta y evaluación sensorial: textura, olor, color y sabor. Resultados. Las propiedades físicas no presentaron diferencias (p>0,05), pero sí (p<0,05) para las variables de color en todos los panes, al igual que en los análisis químicos y perfil de aminoácidos (p<0,05), incrementándose la proteína (15,63 % pan con 7,5 % de HC hasta 23,27 % pan con 12,5 % de HC) y mismo comportamiento de aminoácidos indispensables conforme aumentó la inclusión de HC. Los resultados de la evaluación sensorial fueron similares (p>0,05) para 7,5 % de HC (me gusta), para 10,0 y 12,5 % fue indiferente. Conclusiones. La inclusión de HC (7,5 %) en baguette logró un aumento de proteínas y aminoácidos esenciales mejorando la calidad nutricional, con buena aceptación de acuerdo con la prueba de carácter exploratorio con consumidores que se empleó(AU)

Introduction. Cereals have limiting amino acids and mixing them with flours of marine origin could provide a better balance in these improving the nutritional composition in bakery products, mainly protein and amino acids. Objective. To develop and evaluate the physical, chemical, and sensory quality of three white breads of baguette type by partially substitution of wheat flour (HT) with squid flour (HC). Materials and methods. The sponge dough technique was used incorporating 7,5, 10,0 and 12,5 % of HC. Physical analysis: kneading time, dough weight, dough fermentation, specific volume, bread weight (g), % loss of moisture and crumb color; chemical analysis: moisture, crude protein, ash, ether extract, crude fiber, carbohydrates, indispensable amino acids and sensory evaluation: texture, odor, color, and flavor. Results. Physical properties did not show differences (p>0.05), differences (p<0.05) for color variables in all breads as well as in chemical analysis and amino acid profile (p<0.05), the protein show an increase (15,63 % for bread with HT to 23,27 % for bread with 12.5 % of HC) and the same behavior for indispensable amino acids, that increase as inclusion of HC increases. The results of the sensory evaluation were similar (p>0.05) in control and 7,5 % of HC (I like it), for 10 and 12,5% it was indifferent. Conclusions. The inclusion of squid flour (7,5 %) in the preparation of baguette bread achieved an increase of proteins and essential amino acids that improved the nutritional quality of this food, which also presented a good acceptance according to an exploratory test with consumers(AU)

HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances-ex vivo stratum corneum) protocol: Selection of the subjects and approach to present the results.

OBJECTIVE: Nowadays, it is recognized the need for improved safety and efficacy protocols to evaluate the human stratum corneum (SC) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies. The aim of our research work was to streamline the HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances-ex vivo stratum corneum) methodology, by exploring the results of a group of 18 subjects. METHODS: The study included nine women and nine men aged between 19 and 57 years old with phototypes from II to V. Sites in the forearm of each volunteer were randomly delimited, and the SC was collected by tape stripping. HPLC was used to quantify the MDA-TBA2 (malondialdehyde-thiobarbituric acid) adduct from the tape-stripped SC, irradiated and not by an ultraviolet (UV) simulator chamber. RESULTS: Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous. CONCLUSIONS: It seemed that in future studies focusing on the impact of SC UV-induced lipid peroxidation, determined by the HPLC-TBARS-EVSC, the most suitable subjects are females aged less than 35 years old, with phototype II.

OBJECTIF: Aujourd'hui, il est nécessaire d'améliorer les protocoles de sécurité d'emploi et d'efficacité pour évaluer le stratum corneum (SC) humain et son interaction avec les formulations topiques et cosmétiques par des méthodologies peu ou pas invasives. L'objectif de notre travail de recherche était de rationaliser la méthodologie HPLC-TBARS-SCEV, à savoir chromatographie en phase liquide à haute performance (High Performance Liquid Chromatography), substances réactives à l'acide thiobarbiturique (Thiobarbituric Acid Reactive Substances), stratum corneum ex vivo (SCEV), en explorant les résultats d'un groupe de 18 sujets. MÉTHODES: L'étude incluait 9 femmes et 9 hommes âgés de 19 à 57 ans présentant des phototypes II à V. Des sites de l'avant-bras de chaque volontaire ont été délimités de manière aléatoire, et le SC a été recueilli par « tape stripping ¼. La chromatographie en phase liquide à haute performance a été utilisée pour quantifier l'adduit MDA-TBA2 (malondialdéhyde - acide thiobarbiturique) à partir du SC recueilli par « tape stripping ¼, irradié et non irradié par une chambre de simulation à ultraviolets (UV). RÉSULTATS: En observant les résultats de notre recherche actuelle et l'approche statistique appliquée, l'utilisation du rapport entre le site traité et le site contrôle serait une stratégie adéquate pour mieux discriminer et évaluer les résultats. En outre, une sélection optimale des volontaires pour répondre spécifiquement à l'objectif du test ex vivo peut également être considérée comme bénéfique. CONCLUSIONS: Il semble que dans les études futures axées sur l'impact de la peroxydation lipidique induite par UV du SC, déterminé par la méthodologie HPLC-TBARS-SCEV, les sujets les plus appropriés sont les femmes âgées de moins de 35 ans présentant un phototype II.

First report on quality and purity assessment of sweet almond oil in Brazilian body oils by gas chromatography and mass spectrometry.

Sweet almond oil is a raw material with high-added value used in different products. Then, the aim of this study is to evaluate the quality and purity of 10 body oils based on sweet almond oils currently available in the Brazilian market. Fatty acid composition and triacylglycerol (TAG) profile were determined by gas chromatography with flame ionization detector (GC-FID) and atmospheric solids analysis probe mass spectrometry (ASAP-MS), respectively. The authenticity of samples was assessed using an analytical curve equation. Soybean oil was chosen as the adulterant because it is the cheapest vegetable oil commercialized in Brazil. Hierarchical clustering analysis (HCA) in conjunction with ASAP-MS classified product samples according to the type of vegetable oil (soybean and sweet almond oils). The addition of soybean oil (8.79% to 99.70%) was confirmed in samples. However, only two samples stated in their label the presence of soybean oil as an ingredient. These findings highlight the need for better oversight by regulatory bodies to ensure that consumers acquire high quality and authentic products based on equally high quality and purity of sweet almond oils.

Energy values and metabolizability coefficients of maize kernels with different specific gravities / Valores energéticos e coeficientes de metabolizabilidade de grãos de milho com diferentes densidades específicas

The aim of this study was to examine the influence of the specific gravity of maize kernels on physicochemical traits, energy values, and metabolizability coefficients. Pearson's correlations were evaluated between specific gravity (kg/m3) and crude protein (%); ether extract (%); crude fiber (%); gross energy (%); presence of fumonisins (ppb) and aflatoxins (ppb); and kernel quality (good, rotten, weevil-damaged, broken, and shriveled kernels, %). A metabolism trial was conducted with diets containing maize fractions of different specific gravities for male broilers from 14 to 21 days of age. Apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and the metabolizability coefficients of dry matter (MCDM), crude protein (MCCP), ether extract (MCEE), calcium (MCCa), and phosphorus (MCP) were evaluated. The experiment consisted of five treatments (reference diet and diets with 40% replaced with maize at five specific gravities (740, 740, 760, or 800 kg/m3). Eight replications were used, totaling 400 broilers chickens. The Scott-Knott test was applied and regression equations were fitted to compare the treatments. Specific gravity had moderate correlations with good and broken kernels and low-magnitude correlations with chemical parameters. Increasing specific gravities caused AME and AMEn to increase linearly when analyzed on an as-is basis; and to respond quadratically when expressed on a dry-matter basis. The specific gravity of 780 kg/m3 provided the lowest MCDM, MCCP, MCCa, and MCP values, whereas the lowest MCEE, was found at the lowest density. It was not possible to determine the best nutritional composition or the best metabolizability coefficients.(AU)

Objetivou-se avaliar a influência da densidade do grão de milho sobre características físico-químicas, valores energéticos e de metabolizabilidade aparente. Avaliou-se a correlação entre a densidade específica (kg/m3) e os parâmetros: proteína bruta (%); extrato etéreo (%); fibra bruta (%); energia bruta (%); presença de fumonisinas (ppb) e aflatoxinas (ppb); umidade (%); grãos bons, quebrados, chochos, carunchados e ardidos, em Percentagem. Um ensaio de metabolismo com dietas contendo diferentes densidades específicas do grão de milho, para frango de corte, no período de 14 a 21 dias de idade, foi conduzido para avaliar a energia metabolizável aparente (EMA) e a corrigida pelo balanço de nitrogênio (EMAn), os coeficientes de metabolizabilidade: matéria seca (CM_MS), proteína bruta (CM_PB), extrato etéreo (CM_EE), cálcio (CM_Ca) e fósforo (CM_P). O experimento foi composto por cinco tratamentos (dieta referência e dietas substituindo 40% desta com milhos de densidades específicas: 740 kg/m3, 760 kg/m3, 780 kg/m3 e 800 kg/m3), com oito repetições, totalizando 400 frangos machos. As médias foram comparadas pelo teste de Scott-knott e estimada as equações de regressão nas diferentes densidades específicas das frações de milho. As correlações entre a densidade específica e grãos bons e quebrados foram de moderada magnitude e com os parâmetros químicos foram de baixa magnitude. Com o aumento da densidade específica para EMA e EMAn na matéria natural obteve-se resposta linear decrescente, enquanto na matéria seca, foi quadrático. Para CMMS, CMPB, CMCA e CMP a densidade de 780 kg/m3 apresentou os menores valores, enquanto para CMEE, o menor valor foi para a menor densidade específica. Não foi possível determinar a fração de milho com melhor composição nutricional e melhores coeficientes de metabolizabilidade.(AU)

Detection of adaptive genetic diversity and chemical composition in date palm cultivars and their implications in controlling red palm weevil, Rhynchophorus ferrugineus Oliver / Detecção da diversidade genética adaptativa e composição química em cultivares de tamareira e implicações no controle do bicudo vermelho da palmeira, Rhynchophorus ferrugineus Oliver

This study, about RPW and date palms, is under the scope of date palm bioecology and nutrition (nutritional ecology) which includes the integration of several areas of research such as date palm biochemistry, genetics, and RPW infestation behavior through various date palm cultivars. Date palm (Phoenix dactylifera L.; Arecaceae) production is under threat from the red palm weevil (RPW), Rhynchophorus ferrugineus Oliver. A better understanding of genetic diversity within date palm cultivars can be useful for its implementation within the insect IPM program in the future. Three indices, namely simple-sequence repeats (SSR) markers to elucidate genetic diversity, chemical components, and a natural infestation index of RPW, were used to evaluate the resistant or susceptible date palm cultivars in Qassim. Based on a field survey of RPW infestation within 79 date palm farms involving 11 cultivars at Qassim, the sensitivity and resistance cultivars were determined. The resistant date palm cultivars were Nabtat Ali, Shakrah, red Sukary, and um Kobar which had the lowest degree of RPW abundance %. Values of the essential minerals, nitrogen, phosphorus, potassium, and calcium within the date palm cultivars were also estimated. RPW abundance % was negatively correlated with the calcium content of date palm cultivars. The principal component analysis (PCA) revealed that the calcium content and RPW abundance % were highly affected by the cultivars. SSR markers of the date palm cluster tree divided genotypes into two main groups at similarity coefficients between 0.56 and 0.91. The 1st group included; Nabtet Ali, Red Sukary, Um Kobar, and Shakrah with similarity coefficients between 0.56, this group was the most resistant cultivars. Therefore, SSR markers were able to characterize and resolve genetic diversity in date palm cultivars for RPW resistance. When SSR markers coupled with higher calcium (Ca) content can efficiently replace indices in characterizing resistant date-palm genotypes with a high confidence level. Integration between date palm genetic diversity, chemical structures, and RPW infestations rates promoted the understanding of the interplay between the diversity of RPW management (short-time scale), and the resistance genes, plant nutrition, and dynamics of the diversity of RPW through domestication and diversification (long-timescale). Therefore, our results may lead to a change in RPW control strategies by switching to using safe alternative pesticide control methods (Resistant cultivars of date palm), which are underestimated and may reveal the impact of low-cost, but highly effective agricultural practices in the field of date production in the world. Understanding the genetic structure and calcium content of date palm cultivars mechanisms could help to predict date palm resistance against RPW populations in the new IPM strategy in RPW control.

Este estudo, sobre RPW e tamareiras, está no âmbito da bioecologia e nutrição da tamareira (ecologia nutricional) que inclui a integração de várias áreas de pesquisa, como bioquímica da tamareira, genética e comportamento de infestação de RPW através de vários cultivares de tamareira. A produção da tamareira (Phoenix dactylifera L.; Arecaceae) está ameaçada pelo gorgulho vermelho da palmeira (RPW), Rhynchophorus ferrugineus Oliver. A compreensão mais aprofundada da diversidade genética dentro dos cultivares de tamareiras pode ser útil para sua implementação no futuro programa de MIP de insetos. Três índices, ou seja, marcadores de sequência simples (SSR) para elucidar a diversidade genética, componentes químicos e um índice de infestação natural de RPW, foram utilizados para avaliar as cultivares de tamareiras resistentes ou suscetíveis em Qassim. Com base em uma pesquisa de campo da infestação de RPW em 79 fazendas de tamareiras envolvendo 11 cultivares em Qassim, as cultivares de sensibilidade e resistência foram determinadas. As cultivares de tamareiras resistentes foram Nabtat Ali, Shakrah, red Sukary e um Kobar, que apresentaram o menor grau de abundância de RPW. Também foram estimados os valores dos minerais essenciais, nitrogênio, fósforo, potássio e cálcio nas cultivares de tamareira. A porcentagem de abundância de RPW correlacionou-se negativamente com o teor de cálcio das cultivares de tamareira. A análise de componentes principais (PCA) revelou que o teor de cálcio e a abundância de RPW % foram altamente afetados pelas cultivares. Marcadores SSR da tamareira dividiram os genótipos em dois grupos principais com coeficientes de similaridade entre 0,56 e 0,91. O 1º grupo incluiu; Nabtet Ali, Red Sukary, Um Kobar e Shakrah com coeficientes de similaridade entre 0,56, este grupo foi o de cultivares mais resistentes. Portanto, os marcadores SSR foram capazes de caracterizar e resolver a diversidade genética em cultivares de tamareiras para resistência a RPW. Quando os marcadores SSR associados ao maior teor de cálcio (Ca) podem substituir com eficiência os índices na caracterização de genótipos de tamareiras resistentes com alto nível de confiança. A integração entre diversidade genética da tamareira, estruturas químicas e taxas de infestação de RPW promoveu a compreensão da interação entre a diversidade de manejo de RPW (escala de tempo curto) e os genes de resistência, nutrição de plantas e dinâmica da diversidade de RPW por meio da domesticação e diversificação (longo prazo). Portanto, nossos resultados podem levar a uma mudança nas estratégias de controle de RPW, passando a usar métodos alternativos seguros de controle de pesticidas (cultivares resistentes de tamareira), sendo subestimados e podem revelar o impacto de práticas agrícolas de baixo custo, mas altamente eficazes no campo de produção de tâmaras no mundo. Compreender a estrutura genética e o teor de cálcio dos mecanismos dos cultivares de tamareira pode ajudar a prever a resistência da tamareira contra populações de RPW na nova estratégia de IPM no controle de RPW.

Deep learning for analysis of changes in vital capacity and blood markers after swimming matches based on blended learning / Aprendizado profundo para análise das alterações da capacidade vital e marcadores sanguíneos após jogos de natação baseados no aprendizado combinado / Aprendizaje profundo para el análisis de los cambios en la capacidad vital y los marcadores sanguíneos después de los partidos de natación basados en el aprendizaje combinado

ABSTRACT Introduction Nowadays, more people are concerned with physical exercise and swimming competitions, as a major sporting event, have become a focus of attention. Such competitions require special attention to their athletes and the use of computational algorithms assists in this task. Objective To design and validate an algorithm to evaluate changes in vital capacity and blood markers of athletes after swimming matches based on combined learning. Methods The data integration algorithm was used to analyze changes in vital capacity and blood acid after combined learning swimming competition, followed by the construction of an information system model to calculate and process this algorithm. Results Comparative experiments show that the neural network algorithm can reduce the calculation time from the original initial time. In the latest tests carried out in about 10 seconds, this has greatly reduced the total calculation time. Conclusion According to the model requirements of the designed algorithm, practical help has been demonstrated by building a computational model. The algorithm can be optimized and selected according to the calculation model according to the reality of the application. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Atualmente, mais pessoas preocupam-se com o exercício físico e as competições de natação, como evento esportivo de destaque, tornou-se foco de atenção. Tais competições exigem atenção especial aos seus atletas e o uso de algoritmos computacionais auxiliam nessa tarefa. Objetivo Projetar e validar um algoritmo para avaliação das alterações da capacidade vital e marcadores sanguíneos dos atletas após os jogos de natação baseados no aprendizado combinado. Métodos O algoritmo de integração de dados foi usado para analisar as mudanças de capacidade vital e ácido sanguíneo após competição de natação de aprendizado combinado, seguido à construção de um modelo de sistema de informação para calcular e processar esse algoritmo. Resultados Experiências comparativas mostram que o algoritmo de rede neural pode reduzir o tempo de cálculo a partir do tempo inicial original. Nos últimos testes levados à cabo em cerca de 10 segundos, isto reduziu muito o tempo total de cálculo. Conclusão De acordo com os requisitos do modelo do algoritmo projetado, foi demonstrada a ajuda prática pela construção de um modelo computacional. O algoritmo pode ser otimizado e selecionado de acordo com o modelo de cálculo, segundo a realidade da aplicação. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Hoy en día, cada vez más personas se preocupan por el ejercicio físico y las competiciones de natación, como evento deportivo destacado, se han convertido en un foco de atención. Estas competiciones requieren una atención especial para sus atletas y el uso de algoritmos computacionales ayuda en esta tarea. Objetivo Diseñar y validar un algoritmo para evaluar los cambios en la capacidad vital y los marcadores sanguíneos de los atletas después de los partidos de natación basado en el aprendizaje combinado. Métodos Se utilizó el algoritmo de integración de datos para analizar los cambios de la capacidad vital y la acidez de la sangre tras la competición de natación de aprendizaje combinado, seguido de la construcción de un modelo de sistema de información para calcular y procesar este algoritmo. Resultados Los experimentos comparativos muestran que el algoritmo de la red neuronal puede reducir el tiempo de cálculo con respecto al tiempo inicial. En las últimas pruebas realizadas en unos 10 segundos, esto redujo en gran medida el tiempo total de cálculo. Conclusión De acuerdo con los requisitos del modelo del algoritmo diseñado, se ha demostrado la ayuda práctica mediante la construcción de un modelo computacional. El algoritmo puede optimizarse y seleccionarse según el modelo de cálculo en función de la realidad de la aplicación. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

The impact of an early intervention home-based program on body composition in preterm-born preschoolers with very low birth weight.

Background and aims: Early child interventions focused on the family prevented neurodevelopmental and behavioral delays and can provide more knowledge regarding responsive feeding, thus creating learning opportunities to promote better quality nutrition and preventing failure to thrive. The aim is to verify the impact of a continuous program of early home-based intervention on the body composition of preschool infants who were born preterm with very low birth weight (VLBW). Methods: This is a longitudinal analysis from a randomized controlled trial, including VLBW preterm children, born in a tertiary hospital in Southern Brazil and followed up at the high-risk institutional ambulatory clinic. Participants were divided into the intervention group (IG): skin-to-skin care with the mother (kangaroo care), breastfeeding policy, and tactile-kinesthetic stimulation by mothers until hospital discharge. Subsequently, they received a program of early intervention with orientation and a total of 10 home visits, independently from the standard evaluation and care that was performed following the 18 months after birth; conventional group (CG): standard care according to the routine of the newborn intensive care unit (NICU), which includes kangaroo care, and attending to their needs in the follow-up program. Body composition estimation was performed using bioelectrical impedance analyses (BIA), and physical activity and feeding practices questionnaires were evaluated at preschool age, as well as anthropometric measurements and biochemical analysis. Results: Data of 41 children at 4.6 ± 0.5 years old were evaluated (CG n = 21 and IG n = 20). Body weight, height, body mass index, waist and arm circumferences, and triceps and subscapular skinfold did not differ between groups. The IG presented higher segmented fat-free mass (FFM) when compared to the CG (right arm FFM: 0.74 vs. 0.65 kg, p = 0.040; trunk FFM: 6.86 vs. 6.09 kg, p = 0.04; right leg FFM: 1.91 vs. 1.73 kg, p = 0.063). Interaction analyses showed that segmented FFM and FFM Index were associated with higher iron content in the IG. In the CG, interaction analyses showed that increased visceral fat area was associated with higher insulin resistance index. Conclusion: An early intervention protocol from NICU to a home-based program performed by the mothers of VLBW preterm children of low-income families presents a small effect on FFM.

Preparation and characterization of sequilhos-type biscuits added with almond flour of Acrocomia intumescens.

Acrocomia intumescens ("macaúba") is a species that during processing generates a large amount of waste. The use of this residue for the production of flour for incorporation into food products is a way to minimize the cost and nutritionally enrich the final product. This work aimed to develop and analyze, in terms of physico-chemical and microbiological properties, cookies with macaúba almond cake residual flour. Sequilhos formulations were obtained using three different proportions of flour (2, 4 and 6%). The analysis of the flour allowed to find 4.29% of moisture, 1.13% of acidity, 5.33 of pH, 44.46% of carbohydrates, 28.74% of lipids, 20.06% of proteins and 2.45% of ash. In the analysis of the sequilhos formulations, the following values were found: moisture (5.03 to 8.13%), acidity (0.10 to 0.14%), pH (5.52 to 5.93), carbohydrates (67.17 to 73.37%), lipids (18.77 to 31.77%), proteins (0.85 to 1.92%), ash (0.83 to 0.94%) and total energy value (137.57 to 172,50 Kcal/100g). In microbiological analyzes it was highlighted that the sequilhos presented adequate sanitary conditions. The results indicate almond flour as an ingredient to be incorporated in the preparation of sequilhos, which in turn, presented satisfactory physico-chemical properties and microbiological results.

Post-Partum Depression Lactating Rat Model for Evaluating Ketamine's Safety as a Pharmacotherapeutic Treatment: Roles in Cardiac and Urinary Function.

Depression is one of the world's most common and mentally disabling illnesses. Post-partum depression is a subtype of depression that affects one in seven women worldwide. Successful pharmacological treatment must consider the consequences for both, since the mother-child bond is fundamental for the well-being of both mother and infant as well as the general development of the newborn. Changes in maternal physiology and/or behavior can significantly influence the development of breastfed infants. Ketamine has been extensively studied for use as an antidepressant due to its mixed mechanisms of action. Safety and efficacy studies in the cardiovascular and urinary systems of a lactating postpartum depression animal model are essential for contributing toward ketamine's clinical use in the respective patient population. Thus, this project aimed to study the implications of postpartum maternal exposure to ketamine during lactation on the cardiovascular system of female rats submitted to the depression induction model by maternal separation. This model promotes depressive effects through stress caused by the interruption of mother-infant bond early in the offspring's life. To achieve depression, each dam was separated from her offspring for 3 h per day, from post-natal day 2 (PND2) to PND12. Experimental groups received daily treatment with either 5, 10, or 20 mg/kg of ketamine intraperitoneally during the lactation period, from PND2 to PND21. Behavioral tests consisted of the maternal and aggressive maternal behavior tests, the olfactory preference test, and the forced swim test. A technique for the detection of catecholamines and indoleamines in the heart muscle was developed for the experimental model groups. The histopathological evaluation was performed on these animals' cardiac muscles and urinary bladders. Our findings suggest that ketamine is safe for use in postpartum depression and does not induce cardiovascular and/or urinary systems toxicity.