Building a Tailored Frame-Channel Structure for High-Performance Protein Air Filters.

To create a healthier indoor environment via sustainable technologies, there is a growing demand for constructing high-performance air filters from natural materials. Addressing this need, we have fabricated high-performance protein air filters with a tailored frame-channel structure via electrospinning. The innovative feature of the protein air filter is generated by adding a small amount of an organic salt, tetrabutylammonium chloride (TBAC), to modulate the denaturation of zein for tuning electrical charge distribution and hydrophilicity of the protein solutions. The results highlight that the optimized filter with 1.0 wt% TBAC exhibits a denser nanofiber assembly on the frame and a sparser arrangement on the channel. Functionally, the filter demonstrates ultralow pressure drop (ca. 9.04 Pa) that is only a third of that observed in unmodified formulation and commercial air filters, while it maintains high filtration efficiency in capturing PM2.5 (99.42% ± 0.30%) and PM0.3 (98.25 ± 0.39%). More importantly, the filter indicates multifunctional perspectives, e.g., high removal efficiency for formaldehyde (HCHO) and PM2.5 under high airflow rates (up to 8 L/min) or after prolonged testing period (120 min). Our design of the frame-channel structure for the protein air filter marks a leap forward in developing biomass-based structural materials.

Effects of biochar and compost on the abundant and rare microbial communities assembly and multifunctionality in pesticide-contaminated soil under freeze‒thaw cycles.

Biochar and compost are effective ways to improve soil quality and reduce pesticide pollution. However, the effects of them on the abundant and rare microbial communities in freeze‒thaw soil need to be further clarified. Therefore, this study took biochar, compost, and their combination as examples to explore their effects on the abundant and rare microbial communities and multifunctionality in glyphosate, imidacloprid and pyraclostrobin contaminated soil under freeze‒thaw cycles. We found that freeze‒thaw cycles enhanced the functional groups and surface aromaticity of biochar and compost, thereby improving the adsorption capacity. Biochar and compost reduced the concentration and half-life of three pesticides and enhanced the degradation function of rare taxa in soil. Biochar and compost improved the structure composition and co-occurrence relationship of abundant and rare taxa. Meanwhile, the assembly processes of abundant and rare sub-communities were mainly driven by stochastic processes and the Combined treatment promoted the transition from dispersal limitation to homogenizing dispersal and homogeneous selection. Moreover, the Combined treatment significantly improved the multifunctionality before and after freezing and thawing by increasing the diversity of rare taxa and assembly processes. The results provide new insights for farmland soil remediation in seasonal frozen areas, especially the soil functional cycle of abundant and rare microorganisms.

A multi-functional coating on cotton fabric to incorporate electro-conductive, anti-bacterial, and flame-retardant properties.

Multi-functional textiles have become a growing trend among smart customers who dream of having multiple functionalities in a single product. Thus, this study aimed to develop a multi-functional textile from a common textile substrate like cotton equipped with electrically conductive, anti-bacterial, and flame-retardant properties. Herein, a bunch of compounds from various sources like petro-based poly-aniline (PANI), phosphoric acid (H3PO4), inorganic silver nanoparticles (Ag-NPs), and biomass-sourced fish scale protein (FSP) were used. The coating was prepared via in-situ polymerization of PANI with the cotton substrate, followed by the dipping in AGNPs solution, layer-by-layer deposition of FSP and sodium alginate, and finally, a dip-dry-cure technique after immersing the modified cotton substrate into the H3PO4 and citric acid solution. The key results indicated that the fabric treated with PANI/Ag-NPs/FSP/P-compound exhibited a balanced improvement in all three desired properties as the electrical resistance was reduced by 44.44 % while showing superior bacterial inhibition against gram-positive bacteria (S. aureus) and gram-negative bacteria (E. coli), and produced dense-black carbonaceous char residues, indicating its flame retardant properties as well. Thus, such amicable developments made the cotton textile substrate a multi-functional textile, which showed potential to be used in medical textiles, wearable electronics, fire-fighter suits, etc.

Synthesis and characterization of methacryl glycol chitosan as a novel functionally advanced thermogel for biomedical applications.

Thermo-responsive hydrogels (thermogels), known for their sol-gel transition capabilities, have garnered significant interest for biomedical applications over recent decades. However, conventional thermogels are hindered by intrinsic physicochemical and functional limitations that impede their broader utility. This study introduces methacryl glycol chitosan (MGC) as a novel thermogel, offering enhanced functionality and addressing these limitations. MGCs, synthesized through N-methacrylation of glycol chitosan, exhibit tunable thermogelling and photo-crosslinking behaviors. The thermo-reversible sol-gel transition of MGCs occurs within a 21-54 °C range, adjustable by polymer concentration and methacryl substitution degree. Photo-crosslinking using UV light further enhances the mechanical properties of MGC thermogels, creating thermo-irreversible, chemically crosslinked hydrogels. MGCs show no cytotoxic effects and effectively support cell encapsulation. In vivo studies demonstrate stable crosslinking with minimal UV-induced skin damage. Due to their unique thermo-sensitivity, multi-functionality, and customizable properties, MGC thermogels are promising novel biomaterials for various biomedical applications, particularly injectable tissue engineering and cell encapsulation, thus overcoming the limitations of conventional thermogels.

A self-healing, long-lasting adhesive, lignin-based polyvinyl alcohol organo-hydrogel for strain-sensing applications.

Hydrogel-based flexible sensors have garnered considerable interest in the fields of soft electronics, robotics, and human-machine interfaces. For better practical applications, integrating multiple properties-such as self-adhesive, anti-freeze, anti-volatile, self-healing, and antibacterial-into a single gel for flexible sensors remains a challenge. In this paper, a multifunctional lignin-based polyvinyl alcohol gel, containing dynamic covalent bonds, hydrogen bonds, and coordination bonds, is constructed by a simple one-pot method, in which ethylene glycol/water chosen as a binary solvent and KI as a conductive medium. The resulting organogel exhibits self-healing, long-lasting adhesion, UV shielding, antibacterial properties, excellent frost resistance (-20 °C), and volatile resistance properties. In addition, the organogel-based sensor demonstrates satisfactory sensitivity in detecting joint movements and facial expressions. This study provides a new strategy for developing a versatile flexible sensor through the introduction of renewable and bio-based lignin, promising applications in the fields of wearable electronics.

Long-term contamination of decabromodiphenyl ether reduces sediment multifunctionality: Insights from nutrient cycling, microbial ecological clusters, and microbial co-occurrence patterns.

Despite the widespread detection of polybrominated diphenyl ethers in aquatic ecosystems, their long-term effects on sediment multifunctionality remain unclear. Herein, a 360-day microcosm experiment was conducted to investigate how decabromodiphenyl ether (BDE-209) contamination at different levels (0.2, 2, and 20 mg/kg dry weight) affects sediment multifunctionality, focusing on carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) cycling. Results showed that BDE-209 significantly increased sediment total organic carbon, nitrate, ammonium, available phosphorus, and sulfide concentrations, but decreased sulfate. Additionally, BDE-209 significantly altered key enzyme activities related to nutrient cycling. Bacterial community dissimilarity was positively correlated with nutrient variability. Long-term BDE-209 exposure inhibited C degradation, P transport and regulation, and most N metabolic pathways, but enhanced C fixation, methanogenesis, organic P mineralization, inorganic P solubilization, and dissimilatory sulfate reduction pathways. These changes were mainly regulated by microbial ecological clusters and keystone taxa. Overall, sediment multifunctionality declined under BDE-209 stress, primarily related to microbial co-occurrence network complexity and ecological cluster diversity. Interestingly, sediment C and N cycling had greater impacts on multifunctionality than P and S cycling. This study provides crucial insights into the key factors altering multifunctionality in contaminated sediments, which will aid pollution control and mitigation in aquatic ecosystems.

CTAB-crafted ZnO nanostructures for environmental remediation and pathogen control.

This study addresses the critical need for efficient and sustainable methods to tackle organic pollutants and microbial contamination in water. The present work aim was to investigate the potential of multi-structured zinc oxide nanoparticles (ZnO NPs) for the combined photocatalytic degradation of organic pollutants and antimicrobial activity. A unique fusion of precipitation-cum-hydrothermal approaches was precisely employed to synthesize the ZnO NPs, resulting in remarkable outcomes. The synthesized CTAB/ZnO NPs demonstrated exceptional properties: they were multi-structured and crystalline with a size of 40 nm and possessed a narrow band gap energy of 2.82 eV, enhancing light absorption for photocatalysis. These nanoparticles achieved an impressive degradation efficiency of 91.75% for Reactive Blue-81 dye within 105 min under UV irradiation. Furthermore, their photocatalytic performance metrics were outstanding, including a quantum yield of 1.73 × 10-4 Φ, a kinetic reaction rate of 3.89 × 102 µmol g-1 h-1, a space-time yield of 8.64 × 10-6 molecules photon-1 mg-1, and a figure-of-merit of 1.03 × 10-9 mol L J-1 g-1 h-1. Notably, the energy consumption was low at 1.73 × 10-4 J mol-1, compared to other systems. Additionally, the ZnO NPs exhibited effective antimicrobial activity against S. aureus and P. aeruginosa. This research underscores the potential of tailored ZnO NPs as a versatile solution for addressing both organic pollution and microbial contamination in water treatment processes. The low energy consumption further enhances its attractiveness as a sustainable solution.

Key microbial taxa play essential roles in maintaining soil muti-nutrient cycling following an extreme drought event in ecological buffer zones along the Yangtze River.

Climatic extremes, especially extreme droughts, are occurring more frequently and profoundly impacting biogeochemical processes. However, the relative importance of microbial communities on soil nutrient cycling and community maintenance under natural extreme drought events remains elusive. During a record-breaking drought in the Yangtze River Basin (YRB) in the summer of 2022, we collected ambient soils and drought-affected bare and vegetated soils in ecological buffer zones from two sites with similar soil and vegetation characteristics along the YRB, and examined the relative contribution of soil bacterial communities in supporting multi-nutrient cycling index (MNCI) involving carbon-, nitrate- and phosphorus-cycling and their associations with microbial network. Extreme drought decreased (p < 0.05) bacterial α-diversity but increased MNCI in vegetated soils at both sites, while both remained unchanged (p > 0.05) in bare soils, possibly as a result of vegetation releasing rhizodeposits under drought which selectively recruited bacterial communities. Bacterial community compositions were shifted (p < 0.05) only in vegetated soils, and they exerted more influence than α-diversity on soil MNCI. Notably, the Anaerolineae, identified as a biomarker enriched in vegetated soils, had close associations with enzyme activities and soil MNCI at both sites, suggesting their potential recruitment by vegetation to withstand drought. Furthermore, key ecological clusters (Module 1) in bacterial co-occurrence networks at both sites supported (p < 0.05) higher MNCI, despite no substantial variation in network structure due to drought. Specifically, the most important taxa within Module 1 for predicting soil MNCI revealed by random forest modeling analysis (R2 = 0.44 - 0.63, p < 0.001), such as B1-7BS, SBR1031 and Nocardioides, could be deeply involved in soil nitrogen-cycling, suggesting an essential role of specialized interactions of bacterial communities in maintaining soil multifunctionality. Overall, this study demonstrates that changes in biomarkers and functional taxa under extreme drought may better reflect the biological mechanisms involved in microbial communities impacting ecosystem function, which may aid in forecasting the ecological consequences of ongoing climate change in the ecological buffer zones along the YRB.

Comparative evaluation of the impacts of different microplastics on greenhouse gas emissions, microbial community structure, and ecosystem multifunctionality in paddy soil.

Although the increasing accumulation of microplastics (MPs) in terrestrial soil ecosystems has aroused worldwide concern, research remains limited on their potential impacts on soil processes and ecosystem functionality. Here, through a 41-day microcosm experiment, we found that polylactic acid (PLA), low-density polyethylene (LDPE), and polypropylene (PP) MPs consistently increased soil carbon nutrients and pH but had varying effects on soil nitrogen nutrients and the chemodiversity of dissolved organic matter (DOM). Different treatments led to notable shifts in the α-diversity and composition of soil microbial community, with phyla Proteobacteria and Ascomycota consistently enriched by MPs regardless of polymer type. The emissions of CO2 and N2O were suppressed by MPs in most cases, which in combination led to a decline in global warming potential. LDPE and 1 - 1.5 % of PLA MPs significantly improved the multifunctionality of the soil ecosystem, while PP and 0.5 % of PLA MPs exerted an opposite effect. Soil total organic carbon, pH, DOM molecular mass and condensation degree, and CO2 emissions were identified as the most important variables for predicting soil ecosystem multifunctionality. Results of this study can extend the current understanding of the impacts of MPs on soil biogeochemical cycling and ecosystem functionality.

Unfolding the dynamics of ecosystems undergoing alternating wet-dry transitional states.

A significant fraction of Earth's ecosystems undergoes periodic wet-dry alternating transitional states. These globally distributed water-driven transitional ecosystems, such as intermittent rivers and coastal shorelines, have traditionally been studied as two distinct entities, whereas they constitute a single, interconnected meta-ecosystem. This has resulted in a poor conceptual and empirical understanding of water-driven transitional ecosystems. Here, we develop a conceptual framework that places the temporal availability of water as the core driver of biodiversity and functional patterns of transitional ecosystems at the global scale. Biological covers (e.g., aquatic biofilms and biocrusts) serve as an excellent model system thriving in both aquatic and terrestrial states, where their succession underscores the intricate interplay between these two states. The duration, frequency, and rate of change of wet-dry cycles impose distinct plausible scenarios where different types of biological covers can occur depending on their desiccation/hydration resistance traits. This implies that the distinct eco-evolutionary potential of biological covers, represented by their trait profiles, would support different functions while maintaining similar multifunctionality levels. By embracing multiple alternating transitional states as interconnected entities, our approach can help to better understand and manage global change impacts on biodiversity and multifunctionality in water-driven transitional ecosystems, while providing new avenues for interdisciplinary studies.

Polymer-Assisted Graphite Exfoliation: Advancing Nanostructure Preparation and Multifunctional Composites.

Exfoliated graphite (ExG) embedded in a polymeric matrix represents an accessible, cost-effective, and sustainable method for generating nanosized graphite-based polymer composites with multifunctional properties. This review article analyzes diverse methods currently used to exfoliate graphite into graphite nanoplatelets, few-layer graphene, and polymer-assisted graphene. It also explores engineered methods for small-scale pilot production of polymer nanocomposites. It highlights the chemistry involved during the graphite intercalation and exfoliation process, particularly emphasizing the interfacial interactions related to steric repulsion forces, van der Waals forces, hydrogen bonds, π-π stacking, and covalent bonds. These interactions promote the dispersion and stabilization of the graphite derivative structures in polymeric matrices. Finally, it compares the enhanced properties of nanocomposites, such as increased thermal and electrical conductivity and electromagnetic interference (EMI) shielding applications, with those of neat polymer materials.

Intrinsic Disorder and Other Malleable Arsenals of Evolved Protein Multifunctionality.

Microscopic evolution at the functional biomolecular level is an ongoing process. Leveraging functional and high-throughput assays, along with computational data mining, has led to a remarkable expansion of our understanding of multifunctional protein (and gene) families over the past few decades. Various molecular and intermolecular mechanisms are now known that collectively meet the cumulative multifunctional demands in higher organisms along an evolutionary path. This multitasking ability is attributed to a certain degree of intrinsic or adapted flexibility at the structure-function level. Evolutionary diversification of structure-function relationships in proteins highlights the functional importance of intrinsically disordered proteins/regions (IDPs/IDRs) which are highly dynamic biological soft matter. Multifunctionality is favorably supported by the fluid-like shapes of IDPs/IDRs, enabling them to undergo disorder-to-order transitions upon binding to different molecular partners. Other new malleable members of the protein superfamily, such as those involved in fold-switching, also undergo structural transitions. This new insight diverges from all traditional notions of functional singularity in enzyme classes and emphasizes a far more complex, multi-layered diversification of protein functionality. However, a thorough review in this line, focusing on flexibility and function-driven structural transitions related to evolved multifunctionality in proteins, is currently missing. This review attempts to address this gap while broadening the scope of multifunctionality beyond single protein sequences. It argues that protein intrinsic disorder is likely the most striking mechanism for expressing multifunctionality in proteins. A phenomenological analogy has also been drawn to illustrate the increasingly complex nature of modern digital life, driven by the need for multitasking, particularly involving media.

Temporal asynchrony of plant and soil biota determines ecosystem multifunctional stability.

The role of plant biodiversity in stabilizing ecosystem multifunctionality has been extensively studied; however, the impact of soil biota biodiversity on ecosystem multifunctional stability, particularly under multiple environmental changes, remains unexplored. By conducting an experiment with environmental changes (adding water and nitrogen to a long-term grazing experiment) and an experiment without environmental changes (an undisturbed site) in semi-arid grasslands, our research revealed that environmental changes-induced changes in temporal stability of both above- and belowground multifunctionality were mainly impacted by plant and soil biota asynchrony, rather than by species diversity. Furthermore, changes in temporal stability of above- and belowground multifunctionality, under both experiments with and without environmental changes, were mainly associated with plant and soil biota asynchrony, respectively, suggesting that the temporal asynchrony of plant and soil biota has independent and non-substitutable effects on multifunctional stability. Our findings emphasize the importance of considering both above- and belowground biodiversity or functions when evaluating the stabilizing effects of biodiversity on ecosystem functions.

Phenotypic limits of crop diversity: a data exploration of functional trait space.

Relationships between crop genetic and functional diversity are key to addressing contemporary agricultural challenges. Yet, there are few approaches for quantifying the relationship between genetic diversity and crop functional trait expression. Here, we introduce 'functional space accumulation curves' to analyze how trait space increases with the number of crop genotypes within a species. We explore the potential for functional space accumulating curves to quantify genotype-trait space relationships in four common annual crop species: barley (Hordeum vulgare), rice (Oryza sativa), soybean (Glycine max), and durum wheat (Triticum durum). We also employ these curves to describe genotype-trait space relationships in the wild annual Arabidopsis thaliana, which has not been subjected to artificial selection. All five species exhibited asymptotic functional space accumulation curves, suggesting a limit to intraspecific functional crop diversity, likely due to: dominant phenotypes represented by several genotypes; or functional redundancy that might exist among genotypes. Our findings indicate that there is a diminishing return of functional diversity with increasing number of genotypes. Our analysis demonstrates the efficacy of functional space accumulation curves in quantifying trait space occupancy of crops, with implications for managing crop diversity in agroecosystems, and genetic diversity in crop breeding programs.

Seasonal variations affect the ecosystem functioning and microbial assembly processes in plantation forest soils.

While afforestation mitigates climate concerns, the impact of afforestation on ecological assembly processes and multiple soil functions (multifunctionality) in afforested areas remains unclear. The Xiong'an New Area plantation forests (Pinus and Sophora forests) in North China were selected to examine the effects of plantation types across four distinct seasons on soil microbiomes. Three functional categories (nutrient stocks, organic matter decomposition, and microbial functional genes) of multifunctionality and the average (net) multifunctionality were quantified. All these categories are directly related to soil functions. The results showed that net soil multifunctionality as a broad function did not change seasonally, unlike other narrow functional categories. Bacterial communities were deterministically (variable selection and homogenous selection) structured, whereas the stochastic process of dispersal limitation was mainly responsible for the assembly and turnover of fungal and protist communities. In Pinus forests, winter initiates a sudden shift from deterministic to stochastic processes in bacterial community assembly, accompanied by decreased Shannon diversity and heightened nutrient cycling (nutrient stocks and organic matter decomposition). This indicates the potential vulnerability of deterministic assembly to seasonal fluctuations, particularly in environments rich in nutrients. The results predicted that protist community composition was uniquely structured with C-related functional activities relative to bacterial and fungal ß-diversity variations, which were mostly explained by seasonal variations. Our study highlighted the importance of the protist phagocytosis process on soil microbial interactions through the predicted impact of protist α-diversity on microbial cooccurrence network parameters. This association might be driven by the high abundance of protist consumers as the main predators of bacterial and fungal lineages in our sampling plots. Our findings reveal that the complexity of microbial co-occurrence interactions was considerably higher in spring, perhaps attributing thermal variability and increased resource availability within spring that foster microbial diversity and network complexity. This study contributes to local ecosystem prospects to model the behavior of soil biota seasonally and their implied effects on soil functioning and microbial assembly processes, which will benefit global-scale afforestation programs by promoting novel, precise, and rational plantation forests for future environmental sustainability and self-sufficiency.

Robust Multifunctional Ultrathin 2 Nanometer Organic Nanofibers.

Ultrathin organic nanofibers (UTONFs) represent an emerging class of nanomaterials as they carry a set of favorable attributes, including ultrahigh specific surface area, lightweight, and mechanical flexibility, over inorganic counterparts, for use in biomedicine and nanotechnology. However, precise synthesis of uniform UTONFs (diameter ≤ 2 nm) with tailored functionalities remained challenging. Herein, we report robust multifunctional UTONFs using hydrophobic interaction-driven self-assembly of amphiphilic alternating peptoids containing hydrophobic photoresponsive azobenzene and hydrophilic hydroxyl moieties periodically arranged along the peptoid backbone. Notably, the as-crafted UTONFs are approximately 2 nm in diameter and tens of micrometers in length (an aspect ratio, AR, of ∼10000), exemplifying the UTONFs with the smallest diameter yielded via self-assembly. Intriguingly, UTONFs were disassembled into short-segmented nanofibers and controllably reassembled into UTONFs, resembling "step-growth polymerization". Photoisomerization of azobenzene moieties leads to reversible transformation between UTONFs and spherical micelles. Such meticulously engineered UTONFs demonstrate potential for catalysis, bioimaging, and antibacterial therapeutics. Our study highlights the significance of the rational design of amphiphiles containing alternating hydrophobic and hydrophilic moieties in constructing otherwise unattainable extremely thin UTONFs with ultrahigh AR and stimuli-responsive functionalities for energy and bionanotechnology.

How children come to (not) detect and apply multiple functions for objects: Rethinking perseveration and functional fixedness.

Although humans acquire sophisticated and flexible tool-use skills rapidly throughout childhood, young children and adults still show difficulties using the same object for different functions, manifesting in, for example, perseveration or functional fixedness. This paper presents a novel model proposing bottom-up processes taking place during the acquisition of tool-use abilities through active interaction with objects, resulting in two kinds of cognitive representations of an object: a lower-level, action-centered representation and a higher-level, purpose-centered one. In situations requiring the use of an object to attain a goal, the purpose-centered representation is activated quickly, allowing for an immediate detection of suitable tools. In contrast, activation of the action-centered representation is slow and effortful, but comes with the advantage of offering wide-ranging information about the object's features and how they can be applied. This differential availability and activation of action-centered versus purpose-centered representations also contributes to a deeper understanding of the cognitive mechanisms underlying perseveration or functional fixedness during multifunctional tool use. When applied to the teaching and acquisition of tool use, the model indicates that the form in which object-related information is provided determines which of the two object representations is fostered, thereby either facilitating or complicating the flexible application of an object as a tool for different functions.

Soil keystone viruses are regulators of ecosystem multifunctionality.

Ecosystem multifunctionality reflects the capacity of ecosystems to simultaneously maintain multiple functions which are essential bases for human sustainable development. Whereas viruses are a major component of the soil microbiome that drive ecosystem functions across biomes, the relationships between soil viral diversity and ecosystem multifunctionality remain under-studied. To address this critical knowledge gap, we employed a combination of amplicon and metagenomic sequencing to assess prokaryotic, fungal and viral diversity, and to link viruses to putative hosts. We described the features of viruses and their potential hosts in 154 soil samples from 29 farmlands and 25 forests distributed across China. Although 4,460 and 5,207 viral populations (vOTUs) were found in the farmlands and forests respectively, the diversity of specific vOTUs rather than overall soil viral diversity was positively correlated with ecosystem multifunctionality in both ecosystem types. Furthermore, the diversity of these keystone vOTUs, despite being 10-100 times lower than prokaryotic or fungal diversity, was a better predictor of ecosystem multifunctionality and more strongly associated with the relative abundances of prokaryotic genes related to soil nutrient cycling. Gemmatimonadota and Actinobacteria dominated the host community of soil keystone viruses in the farmlands and forests respectively, but were either absent or showed a significantly lower relative abundance in that of soil non-keystone viruses. These findings provide novel insights into the regulators of ecosystem multifunctionality and have important implications for the management of ecosystem functioning.

The abundant fraction of soil microbiomes regulates the rhizosphere function in crop wild progenitors.

The rhizosphere influence on the soil microbiome and function of crop wild progenitors (CWPs) remains virtually unknown, despite its relevance to develop microbiome-oriented tools in sustainable agriculture. Here, we quantified the rhizosphere influence-a comparison between rhizosphere and bulk soil samples-on bacterial, fungal, protists and invertebrate communities and on soil multifunctionality across nine CWPs at their sites of origin. Overall, rhizosphere influence was higher for abundant taxa across the four microbial groups and had a positive influence on rhizosphere soil organic C and nutrient contents compared to bulk soils. The rhizosphere influence on abundant soil microbiomes was more important for soil multifunctionality than rare taxa and environmental conditions. Our results are a starting point towards the use of CWPs for rhizosphere engineering in modern crops.