Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm.

Bioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat, Shofu, Kyoto, Japan) on periodontopathogenic biofilms. This study evaluated the antibacterial activity of S-PRG fillers on the microbial profile of multispecies subgingival biofilms. A Calgary Biofilm Device (CBD) was used to grow a 33-species biofilm related to periodontitis for 7 days. The S-PRG coating was applied on CBD pins from the test group and photo-activated (PRG Barrier Coat, Shofu), while the control group received no coating. Seven days after treatment, the total bacterial counts, metabolic activity, and microbial profile of the biofilms were observed using a colorimetric assay and DNA-DNA hybridization. Statistical analyses were applied; namely, the Mann-Whitney, Kruskal-Wallis, and Dunn's post hoc tests. The bacterial activity of the test group was reduced by 25.7% compared with that of the control group. A statistically significant reduction was observed for the counts of 15 species: A. naeslundii, A. odontolyticus, V. parvula, C. ochracea, C. sputigena, E. corrodens, C. gracilis, F. nucleatum polymorphum, F. nucleatum vincentii, F. periodonticum, P. intermedia, P. gingivalis, G. morbillorum, S. anginosus, and S. noxia (p ≤ 0.05). The bioactive coating containing S-PRG modified the composition of the subgingival biofilm in vitro, thereby decreasing colonization by pathogens.

Significance of immediate dentin sealing and flowable resin coating reinforcement for unfilled/lightly filled adhesive systems.

BACKGROUND: Immediate dentin sealing implies applying an adhesive system to dentin directly after tooth preparation, before impression. The technique is universal (inlays, onlays, veneers, crowns) and well documented clinically and experimentally. Different types of dentin bonding agents (DBAs) are available on the market. Major differences lie in the thickness of the hybrid layer and overlaying adhesive resin (filled vs. unfilled/lightly filled adhesives). OBJECTIVE: The objective of this work is to provide precise clinical instructions and present new experimental data about the bond strength of five DBAs (Optibond FL, Scotchbond MP, Single Bond Plus, Clearfil SE Bond, and Scotchbond Universal) used conventionally (dentin sealed at the time of restoration delivery) or with immediate dentin sealing, as well as with an additional flowable resin coating. METHODS: Seventy-five human molars were selected, restored/tested according the microtensile bond strength method. Fifteen groups (n=5) were obtained from the combination of the five DBAs and three application modes: delayed dentin sealing, immediate dentin sealing and immediate dentin sealing with flowable resin coating. RESULTS: It appears that immediate dentin sealing was confirmed to significantly improve the bond strength of all tested adhesives. The use of a flowable resin coating reinforcement after immediate dentin sealing increased the microtensile bond strength of all unfilled/lightly filled adhesives (from 233% of increase for ScotchBond MP, up to 560% for Clearfil SE Bond) and maintained the performance of the 3-step golden standard adhesive. Optibond FL used with (52.51 MPa) or without (54.75 MPa) additional flowable resin coating and Clearfil SE Bond (45.64 MPa) used with flowable resin coating provided the best results. CLINICAL SIGNIFICANCE: The original immediate dentin sealing (IDS) technique implies the use of a filled DBA. With unfilled/lightly filled adhesives, it is suggested to reinforce IDS with an additional flowable resin coating. This seems especially paramount to the performance of simplified adhesive systems to protect the thin bonding interface from oxygen inhibition and preserve IDS layer during predelivery cleaning of the preparation. The clinical reinforcement of unfilled/lightly filled IDS with flowable resin composite is encouraged for more predictable bonding.

Microtensile bond strength of indirect composite restorations using different combinations of resin-coating technique / Resistência da união por microtração de restaurações indiretas de resina, usando diferentes combinações na técnica de Resin-Coating

The aim of this study was to evaluate microtensile bond strength (μ-TBS) and failure mode of indirect composite restorations bonded to dentin using different combinations of Resin-Coating (RC) after thermal and load cycling. Thirty five extracted third molars were used in the study. Two box-like Class II cavities were prepared in each tooth (mesial and distal surface). The 70 cavities were distributed in 7 groups according to the coating materials: G1:Etch-rinse 2steps (SB2); G2:Etch-rinse 2steps/Hydrophobic-monomer (SB2/B); G3:Etch-rinse 2steps/Flowable composite resin (SB2/FL); G4:Self-etch 1step (CS3); G5:Self-etch 1step/Hydrophobic monomer (CS3/B); G6:Self-etch1step/Flowable composite resin liner (CS3/PL), G7:Self-etch 2step/Flowable composite resin liner (CSEB/PL). The cavities were molded with a vinyl polysiloxane impression material and the molds were poured with a stone plaster. The fillings were confectioned using the Sinfony composite system (3M/ESPE) and were cemented with resin luting cement (Rely X ARC system). After 24 hours, the teeth were submitted to thermocycling (2,000C/5-55°C) and load cycling (250,000C/30N). After, the restored teeth were sectioned in to beams and μ-TBS were measured. The data were analyzed with ANOVA and Tukey test (p<0.05). In addition failure mode pattern was determined by scanning electrical microscopy. Bond strength were significantly higher in the groups CSEB/PL and CS3/B (p<0.05). In the groups in which was not used a liner, the failure mode exhibited dentin exposure. The groups CSEB/PL and CS3/B showed the highest values of bond strength and the failure mode reveal CSEB/PL exhibited better performance since doesn’t present any fracture kind A.

O objetivo deste estudo foi avaliar a resistência da união, por microtração (μ-TBS), e o modo de fratura de restaurações indiretas de resina aderidas à dentina por diferentes combinações na técnica de Resin-Coating (RC), após ciclagem térmica e mecânica. Neste trabalho foram utilizados trinta e cinco terceiros molares extraídos. Em cada dente foram preparadas duas caixas Classe II (nas faces mesial e distal). As 70 cavidades foram divididas em 7 grupos, de acordo com os materiais de cobertura: G1: adesivo convencional de 2 passos (SB2); G2: adesivo convencional de 2 passos/monômero hidrófobo (SB2/B); G3: adesivo convencional de 2 passos /resina composta flow (SB2/FL); G4:adesivo auto-condicionante de 1 passo (CS3); G5: adesivo auto-condicionante de 1 passo/monômero hidrófobo (CS3/B); G6: adesivo auto-condicionante de 1 passo/resina composta flow forradora (CS3/PL), G7: adesivo auto-condicionante de 2 passos/resina composta flow forradora (CSEB/PL). As cavidades foram moldadas por materiais à base de polivinilsiloxano e os modelos foram obtidos em gesso pedra especial. As restaurações foram confeccionadas usando o sistema Sinfony de compósitos (3M/ESPE); e foram cimentadas com cimento resinoso (Rely X ARC system). Após 24 h, os dentes foram submetidos a ciclagem térmica (2.000C/ 5-55oC) e mecânica (250.000/30N). Foram então seccionadas em palitos para medidas de μ-TBS. Os dados foram analisados por ANOVA e teste Tukey (p<0,05). Além disso, os padrões de fratura foram determinados por meio de microscopia eletrônica de varredura. A resistência da união foi significativamente mais alta nos grupos CSEB/PL e CS3/B (p<0.05). Nos grupos onde não foi usado um agente forrador, os padrões de fratura exibiram exposição de dentina. Os grupos CSEB/PL and CS3/Bos maiores valores de resistência de união e o modo de fratura revelou que CSEB/PL exibiu melhor performance, considerando o fato de não apresentarem nenhuma fratura Tipo A.

Marginal adaptation of indirect restorations using different resin coating protocols

This study evaluated the influence of material combinations used in the resin coating technique (RCT) on the marginal adaptation of indirect restorations with gingival margins in enamel (EM) and cement (CM). Eighty third-molars were used. Two cavities were prepared in each tooth. The cavities were distributed into 16 groups. Cavities with EM were filled with the following material combinations: G1: Single-Bond 2 (Sb2), G2: Sb2 + Bond/Scotchbond-Multipurpose (Sb2B), G3: Sb2 + Filtek-Flow Z350 (Sb2Fl), G4: Scotchbond-Multipurpose (SBMP), G5: Clearfil-S3 (CS3), G6: CS3 + Bond/Clearfil-SE Bond (CSE3B), G7: CS3 + Protect Liner F (CS3PL) and G8: Clearfil SE Bond + Protect Liner F (CSEBPL). The same combinations were applied to the cavities in CM: G9, G10, G11, G12, G13, G14, G15, G16, respectively. The fillings were performed with the Sinfony-System (3M/ESPE). After 24 h, the teeth were submitted to thermocycling (2,000 cycles, 5° to 55°C) and load-cycling (50,000 cycles, 50 N). Next, the Caries-Detector (Kuraray) was applied to the restoration margins. Images from the proximal margin were evaluated using the Image-Tool 3.0 software. The results were submitted to ANOVA and Tukey's test (α=0.05). The mean values (%) for the groups were: EM: G1=46.68, G2=15.53, G3=19.83, G4=27.53; G5=59.49, G6=25.13, G7=34.37, G8=15.20; CM: G9=38.38, G10=23.25, G11=26.97, G12=25.85, G13=37.81, G14=30.62, G15=29.17, G16=20.31. The highest percentages of marginal gap on EM or CM were found in the groups that did not use a liner. It can be concluded that the most appropriate RCT combinations were the groups that used a liner.

Este estudo avaliou a influência de diferentes combinações de materiais usados na técnica de selamento dentinário (TSD) sobre a adaptação marginal de restaurações indiretas, cujas margens gengivais localizam-se em esmalte (ME) ou cemento (MC). Oitenta terceiros molares foram selecionados e duas cavidades foram preparadas em cada dente, as quais foram distribuídas em 16 grupos. As cavidades com margem em esmalte foram forradas pelas seguintes combinações de materiais: G1: Single-Bond2 (Sb2), G2: Sb2 + Bond/Scotchbond-Multipurpose (Sb2B), G3: Sb2 + Filtek-Flow Z350 (Sb2Fl), G4: Scotchbond-Multipurpose (SBMP), G5: Clearfil-S3 (CS3), G6: CS3 + Bond/Clearfil-SE Bond (CSE3B), G7: CS3 + Protect Liner F (CS3PL), G8: Clearfil SE Bond + Protect Liner F (CSEBPL). As mesmas combinações foram aplicadas às cavidades com margens em cemento: G9, G10, G11, G12, G13, G14, G15, G16, respectivamente. As restaurações foram confeccionadas usando o sistema Sinfony (3M/ESPE). Após 24 h, os dentes restaurados foram submetidos à ciclagem térmica (2.000 ciclos - 5° a 55° C) e mecânica (50.000 ciclos, 50 N). Em seguida, Carie-Detector (Kuraray) foi aplicado sobre as margens das restaurações. As imagens obtidas da margem proximal foram avaliadas pelo software Image-Tool 3.0. Os resultados foram submetidos aos testes estatísticos ANOVA e Tukey (p<0,05). As médias (%) observadas para os grupos foram: ME: G1=46,68, G2=15,53, G3=19,83, G4=27,53; G5=59,49, G6=25,13, G7=34,37 e G8=15,20; MC: G9=38,38, G10=23,25, G11=26,97, G12=25,85, G13=37,81, G14=30,62, G15=29,17, G16=20,31. Os maiores valores de desadaptação marginal encontrados em ME e MC foram encontrados nos grupos que não utilizaram um "liner". Desta forma, pôde-se concluir que a combinação mais apropriada para a TSD é aquela que faz uso do "liner".

Resistência de união ao cisalhamento e à tração de uma cerâmica fixada com cimento resinoso a dentina tratada pela técnica do resin-coating / Shear and tensile bond strength of ceramic crowns cemented with resinous cement after the resin coating technique

A técnica resin-coating consiste na aplicação de sistema adesivo eresina de baixa viscosidade flow imediatamente após o preparo cavitário, produzindouma camada híbrida protegendo dentina e tecido pulpar subjacente. O objetivodesta pesquisa foi avaliar a resistência de união ao cisalhamento e à traçãoquando uma restauração foi fixada com cimento resinoso de cura dual à dentinacom resin-coating. Foram comparados os sistemas adesivos autocondicionantes depasso único (ED Primer) e dois passos (Clearfil Protect Bond) e a técnica de limpezado cimento temporário na resistência adesiva. Foram confeccionadas 160amostras. Os grupos foram divididos, para cada sistema adesivo e para cada tipode teste, da seguinte forma: Grupo 1 ? non-resin-coating, sem cimentação temporária;Grupo 2 ? resin-coating, sem cimentação temporária; Grupo 3 ? resin-coating,cimentação temporária, remoção do cimento com colher de dentina e bolinhade algodão embebida em etanol; Grupo 4 ? resin-coating, cimentação temporária,remoção do cimento com jato de bicarbonato e bolinha de algodão embebidaem etanol. A análise estatística utilizou o teste não-paramétrico de Mann-Whitney.Nos resultados obtidos, não houve significância estatística entre o gruponon-resin-coating e o resin-coating submetidos ao teste de cisalhamento, ao contráriodo teste de tração em que o resin-coating aumentou a adesão. O sistema adesivode dois passos mostrou-se mais eficiente que o de passo único. Observou-se,também, que não houve diferença entre as técnicas de limpeza

The resin coating techniqueconsists in adhesive system and flow resincomposite application immediately after cavitypreparation, yielding a hybrid layer and protectingthe subjacent pulpal complex. The aim ofthis study was to evaluate the tensile and shearbond strength of ceramic crowns cemented withresinous cement after the resin coating technique.Two self etching adhesive systems (ED Primer,one step and (Clearfil Protect Bond, two steps)were compared as also different temporary cementremoving techniques. One hundred and sixty specimenswere divided in groups as followed: G1)non-resin coating/no temporary cementation,G2) resin coating/no temporary cementation,G3) resin coating/temporary cement removedwith dentin spoon/ethanol soaked cotton pellet,G4) resin coating/temporary cement removedwith bicarbonate air abrasion/ethanol soakedcotton pellet. Data were submitted to non-parametricalstatistical test (Mann-Whitney). Therewere no different statistical significance betweenthe resin coating and the non-resin coating groupswhen submitted to the shear test. The resin coatinggroups showed higher tensile bond strengththan the others groups. The two steps self etchingadhesive system seemed to improve the bondstrength results. There were no differences amongthe temporary cement removing techniques used