Spatial distribution of load induced soft-tissue strain in cattle claws.

Claw disorders in dairy cattle have negative effects on both animal welfare and farm profits. One possible cause of claw disorders is the high mechanical load that cattle encounter when walking and standing on hard concrete floors. It is currently unclear how high mechanical loading leads to claw disorders and lameness. It is hypothesized that mechanical loading leads to compression of the soft tissue in the claws, which may directly or indirectly lead to tissue damage. Roentgen stereophotogrammetry in combination with CT-reconstruction was used to detect deformations in the distal hind limbs of dissected specimens of dairy cows under a range of loading regimens. The load was recorded in 3D using a force plate. Even at moderate load levels, such as during standing, the soft tissue layer was considerably compressed (>10% of the initial thickness), especially where the sole rests on the floor. Compression increases with increased and/or prolonged load. Most importantly, the location of areas of highest compression coincides with the locations where sole ulcers are most often found. These findings provide insight into the etiology of bovine claw disorders, and may contribute to solutions to reduce them.

A new technique using roentgen stereophotogrammetry to measure changes in the spatial conformation of bovine hind claws in response to external loads.

Claw and locomotion problems are widespread in ungulates. Although it is presumed that mechanical overload is an important contributor to claw tissue damage and impaired locomotion, deformation and claw injury as a result of mechanical loading has been poorly quantified and, as a result, practical solutions to reduce such lesions have been established mostly through trial and error. In this study, an experimental technique was developed that allowed the measurement under controlled loading regimes of minute deformations in the lower limbs of dissected specimens from large ungulates. Roentgen stereophotogrammetric analysis (RSA) was applied to obtain 3D marker coordinates with an accuracy of up to 0.1 mm with optimal contrast and to determine changes in the spatial conformation. A force plate was used to record the applied forces in three dimensions. The results obtained for a test sample (cattle hind leg) under three loading conditions showed that small load-induced deformations and translations as well as small changes in centres of force application could be measured. Accuracy of the order of 0.2-0.3 mm was feasible under practical circumstances with suboptimal contrast. These quantifications of claw deformation during loading improve understanding of the spatial strain distribution as a result of external loading and the risks of tissue overload. The method promises to be useful in determining load-deformation relationships for a wide variety of specimens and circumstances.

Accuracy of noninvasive, single-plane fluoroscopic analysis for measurement of three-dimensional femorotibial joint poses in dogs treated by tibial plateau leveling osteotomy.

OBJECTIVE: To compare accuracy of a noninvasive single-plane fluoroscopic analysis technique with radiostereometric analysis (RSA) for determining 3-D femorotibial poses in a canine cadaver stifle joint treated by tibial-plateau-leveling osteotomy (TPLO). SAMPLE: Left pelvic limb from a 25-kg adult mixed-breed dog. PROCEDURES: A CT scan of the left pelvic limb was performed. The left cranial cruciate ligament was transected, and a TPLO was performed. Radiopaque beads were implanted into the left femur and tibia, and the CT scan was repeated. Orthogonal fluoroscopic images of the left stifle joint were acquired at 5 stifle joint flexion angles ranging from 110° to 150° to simulate a gait cycle; 5 gait cycles were completed. Joint poses were calculated from the biplanar images by use of a digitally modified RSA and were compared with measurements obtained by use of hybrid implant-bone models matched to lateral-view fluoroscopic images. Single-plane measurements were performed by 2 observers and repeated 3 times by the primary observer. RESULTS: Mean absolute differences between results of the single-plane fluoroscopic analysis and modified RSA were 0.34, 1.05, and 0.48 mm for craniocaudal, proximodistal, and mediolateral translations, respectively, and 0.56°, 0.85°, and 1.08° for flexion-extension, abduction-adduction, and internal-external rotations, respectively. Intraobserver and interobserver mean SDs did not exceed 0.59 mm for all translations and 0.93° for all rotations. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that single-plane fluoroscopic analysis by use of hybrid implant-bone models may be a valid, noninvasive technique for accurately measuring 3-D femorotibial poses in dogs treated with TPLO.

Three dimensional, radiosteriometric analysis (RSA) of equine stifle kinematics and articular surface contact: a cadaveric study.

REASONS FOR PERFORMING STUDY: Studies examining the effect of stifle joint angle on tibial rotation, adduction-abduction angle and articular contact area are lacking. OBJECTIVES: To test the hypothesis that tibial rotation, adduction-abduction angle and articular contact area change with stifle joint angle. STUDY DESIGN: Descriptive study of normal kinematics and articular contact patterns of the equine stifle through the functional range of motion using 3 dimensional (3D) radiosteriometric analysis (RSA) and equine cadaver stifles. METHODS: Multiple, radiopaque markers were embedded in the distal femur and proximal tibia and sequential, biplanar x-rays captured as the stifle was passively extended from 110° to full extension. Computer-programmed RSA was used to determine changes in abduction-adduction and internal-external rotation angles of the tibia during stifle extension as well as articular contact patterns (total area and areas of high contact) through the range of motion. RESULTS: The tibia rotated externally (P < 0.001) as the stifle was extended. Tibial abduction occurred from 110-135° of extension (P < 0.001) and tibial adduction occurred from 135° through full extension (P = 0.009). The centre of joint contact moved cranially on both tibial condyles during extension with the lateral moving a greater distance than the medial (P = 0.003). Articular contact area decreased (P = 0.001) in the medial compartment but not in the lateral compartment (P = 0.285) as the stifle was extended. The area of highest joint contact increased on the lateral tibial condyle (P < 0.001) with extension but decreased (P = 0.001) on the medial tibial condyle. CONCLUSIONS: Significant changes occur in tibial rotation, adduction-abduction angle and articular contact area of the equine stifle through the functional range of motion. Understanding the normal kinematics of the equine stifle and the relationship between joint positions and articular contact areas may provide important insight into the aetiology and location of common stifle joint pathologies (articular cartilage and meniscal lesions).