Advances in flow cytometry in basic and applied equine andrology

The aim of this review is to present the current probes available that assess different compartments and functions of stallion spermatozoa, including assays to investigate the functionality of the membranes, nucleus and mitochondria, and to study cell signaling in this particular cell. New multi-parametric protocols for the assessment of stallion sperm, recently developed in the laboratory of the authors, will also be presented. The potential clinical applicability of diagnostic tests based on flow cytometry will also be discussed.(AU)

Advances in flow cytometry in basic and applied equine andrology

The aim of this review is to present the current probes available that assess different compartments and functions of stallion spermatozoa, including assays to investigate the functionality of the membranes, nucleus and mitochondria, and to study cell signaling in this particular cell. New multi-parametric protocols for the assessment of stallion sperm, recently developed in the laboratory of the authors, will also be presented. The potential clinical applicability of diagnostic tests based on flow cytometry will also be discussed.

Avaliação andrológica de ovinos do Núcleo de Ruminantes da UEMA Campus Paulo VI / Evaluation andrologic of Ruminant Center of cattle from UEMA - Campus Paulo VI

The aim of the study was to characterize andrologicaly sheeps belonging to Ruminants Center ofUniversidade Estadual do Maranhao. The semen collection was performed by electro ejaculation. The bodycondition score from seven animals showed averaging 4.57 ± 1.13. The scrotal circumference (SC) was 30.58 ±4.91 cm, a scrotal circumference of rams presented mean length and width of the right testicle of 9.43 ± 1.16 cmand 5.44 ± 0.46 cm and the left testicle of 9.0 ± 0.81 cm and 5.51 ± 0.41 cm, respectively. The average volume ofsemen obtained was 0.89 ± 0.32 mL, appearance, color and odor sui generis were for the species. For physicalsemen parameters, the average turbulence was 3.57 ± 0.53; force of 3.14 ± 0.38; Sperm motility of 81.43 ±6.90%; sperm concentration of 0.8 ± 0.18 x109sptz / mL. The proportion of living and dead sperm stained witheosin-nigrosin averaged 74.1 ± 3.02% and 25 ± 3.46%, respectively. According to this study and the resultsobtained, sheep showed morphology and physical aspects consistent with the species and can be used asbreeders.(AU)

Avaliação andrológica de ovinos do Núcleo de Ruminantes da UEMA – Campus Paulo VI / Evaluation andrologic of Ruminant Center of cattle from UEMA - Campus Paulo VI

The aim of the study was to characterize andrologicaly sheeps belonging to Ruminants Center ofUniversidade Estadual do Maranhao. The semen collection was performed by electro ejaculation. The bodycondition score from seven animals showed averaging 4.57 ± 1.13. The scrotal circumference (SC) was 30.58 ±4.91 cm, a scrotal circumference of rams presented mean length and width of the right testicle of 9.43 ± 1.16 cmand 5.44 ± 0.46 cm and the left testicle of 9.0 ± 0.81 cm and 5.51 ± 0.41 cm, respectively. The average volume ofsemen obtained was 0.89 ± 0.32 mL, appearance, color and odor sui generis were for the species. For physicalsemen parameters, the average turbulence was 3.57 ± 0.53; force of 3.14 ± 0.38; Sperm motility of 81.43 ±6.90%; sperm concentration of 0.8 ± 0.18 x109sptz / mL. The proportion of living and dead sperm stained witheosin-nigrosin averaged 74.1 ± 3.02% and 25 ± 3.46%, respectively. According to this study and the resultsobtained, sheep showed morphology and physical aspects consistent with the species and can be used asbreeders.