RESUMEN
In order to investigate the expression levels of serum IFN-γ, IL-4, and tumor necrosis TNF-α in patients with cervicitis complicated with human papillomavirus (HPV) infection and clinical significance, a retrospective study was conducted on 90 patients with chronic cervicitis complicated by HP V infection who visited our hospital from June 2020 to June 2021, and they are included in the research group. According to the degree of HPV infection, the patients are divided into low-risk HPV type group (n = 65 cases) and high-risk HPV type group (n = 25 cases); 50 patients with cervicitis (without HPV infection) who received treatment in our hospital are selected as control group 1. Fifty healthy women who underwent physical examination are selected as the control group 2. The general data of the two groups of patients during hospitalization are collected, and HPV-DNA, IFN-γ, IL-4, and TNF-α are detected in all patients. For patients with cervicitis complicated by HPV infection, the IFN-indexes in the body are significantly decreased, IL-4 and TNF-αare significantly increased, and with the degree of HPV infection, IFN-γ, IL-4, and TNF-α have high diagnostic performance with HPV infection, and there is a significant correlation between the three, which can be used in cervicitis complicated with HPV infection. It is widely used in the early diagnosis and screening of infected patients.
Asunto(s)
Infecciones por Papillomavirus , Cervicitis Uterina , Femenino , Humanos , Interferón gamma/sangre , Interleucina-4/sangre , Infecciones por Papillomavirus/sangre , Infecciones por Papillomavirus/complicaciones , Infecciones por Papillomavirus/diagnóstico , Estudios Retrospectivos , Factor de Necrosis Tumoral alfa/sangre , Cervicitis Uterina/sangre , Cervicitis Uterina/complicaciones , Cervicitis Uterina/diagnósticoRESUMEN
Cervicitis is an extremely common gynecological disease and can be induced by diverse factors such as Neisseria gonorrhoeae, Chlamydia trachomatis, and Mycoplasma genitalium infections. Long-term unhealed cervicitis may lead to a series of diseases including endometritis, salpingitis, pelvic inflammatory disease, and chorioamnionitis. However, the pathogenesis of cervicitis remains unknown. Ainsliaea fragrans Champ. (AFC) has been widely used in clinical treatment of cervicitis. In the present study, we performed an integrative investigation involving histopathology analysis and non-target plasma metabolomics analysis in a cervicitis rat model induced by phenol mucilage, using ultra-performance liquid chromatography coupled with a tandem quadrupole time-of-flight mass spectrometry approach. Based on the integrative investigation, marked metabolomic differences were identified between the cervicitis and control groups using multivariate analysis. As a result, 32 potential biomarkers were identified in the response to cervicitis, and were involved in arachidonic acid metabolism, linoleic acid metabolism, primary bile acid biosynthesis, taurine and hypotaurine metabolism, pantothenate and CoA biosynthesis, and glycerophospholipid metabolism. After treatment, a total of 27 potential biomarkers exhibited altered levels in the AFC group compared to the model group, and 12 metabolites including 1-stearoylglycerophosphoinositol, bolasterone, lysoPC(16:0), lysoPC(20:4), lysoPC(P-16:0), lysoPC(P-18:0), lysoPC(P-18:1), stearoylcarnitine, taurine, lysoPC(17:0), 20-hydroxyeicosatetraenoic acid, and 1-arachidonoylglycerophosphoinositol returned to their normal levels. This study suggested that the therapeutic mechanism of AFC is related to those altered endogenous metabolites.
Asunto(s)
Asteraceae/química , Medicamentos Herbarios Chinos/farmacología , Metabolismo de los Lípidos/efectos de los fármacos , Cervicitis Uterina/tratamiento farmacológico , Adhesivos/toxicidad , Animales , Biomarcadores/sangre , Cromatografía Líquida de Alta Presión/métodos , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/uso terapéutico , Femenino , Humanos , Lípidos/sangre , Metabolómica/métodos , Fenol/toxicidad , Ratas , Ratas Sprague-Dawley , Organismos Libres de Patógenos Específicos , Espectrometría de Masas en Tándem/métodos , Cervicitis Uterina/sangre , Cervicitis Uterina/inducido químicamente , Cervicitis Uterina/patologíaRESUMEN
We evaluated whether genital inflammation affects the selection of the transmitted virus. Among South African women, we found that preinfection genital inflammation facilitates transmission of less infectious human immunodeficiency virus, but highly infectious viruses are able to establish infection regardless of inflammation status. This suggests that viral phenotype can influence transmission risk.
Asunto(s)
Infecciones por VIH/complicaciones , Infecciones por VIH/virología , VIH-1/clasificación , VIH-1/genética , Cervicitis Uterina/complicaciones , Vaginitis/complicaciones , Biomarcadores , Citocinas/sangre , Femenino , Infecciones por VIH/sangre , Infecciones por VIH/tratamiento farmacológico , Humanos , Filogenia , Cervicitis Uterina/sangre , Cervicitis Uterina/diagnóstico , Vaginitis/sangre , Vaginitis/diagnóstico , Carga ViralRESUMEN
OBJECTIVE: To identify plasma protein biomarkers of cervical high-grade squamous intraepithelial lesion (HSIL) of Uyghur women by proteomics approach. METHODS: Plasma protein samples of Uyghur women with HSIL and chronic cervicitis were analyzed with 2D HPLC followed by detection of target proteins with Linear Trap Quadrupole Mass Spectrometer (LTQ MS/MS). RESULTS: We detected three upregulated and one downregulated protein peaks representing protein constituents distinguishing HSIL from controls by 2D HPLC, identified 31 target proteins by LTQ MS/MS. Further confirmed analysis with online software IPA® 8.7 and ELISA assay showed APOA1 and mTOR as potential biomarkers. CONCLUSIONS: A distinct plasma proteomic profile may be associated with HSIL of Uyghur women.
Asunto(s)
Apolipoproteína A-I/sangre , Biomarcadores de Tumor/sangre , Serina-Treonina Quinasas TOR/sangre , Displasia del Cuello del Útero/sangre , Neoplasias del Cuello Uterino/sangre , Adulto , Apolipoproteína A-I/aislamiento & purificación , Pueblo Asiatico , Biomarcadores de Tumor/aislamiento & purificación , Proteínas Sanguíneas/aislamiento & purificación , Proteínas Sanguíneas/metabolismo , Neuritis del Plexo Braquial , Cromatografía de Fase Inversa , Detección Precoz del Cáncer , Femenino , Humanos , Péptidos y Proteínas de Señalización Intercelular/sangre , Péptidos y Proteínas de Señalización Intercelular/aislamiento & purificación , Persona de Mediana Edad , Lactógeno Placentario , Proteómica , Serina-Treonina Quinasas TOR/aislamiento & purificación , Espectrometría de Masas en Tándem , Neoplasias del Cuello Uterino/diagnóstico , Cervicitis Uterina/sangre , Displasia del Cuello del Útero/diagnósticoRESUMEN
The blood plasma erythropoietin content, erythropoietin content, erythropoietin and cellular renovation markers expression in exocervical epithelium in reproductive aged women with normal peripheral red blood values were studied. It was determined that erythropoietin expression increased in exocervical epithelium activity (cervical intraepithelial neoplasia 1 and 2), combined with the increase of blood plasma erythropoietin level, activation of pro- and antiapoptosis programs and cellular proliferation processes in cervical epithelium.
Asunto(s)
Apoptosis , Proliferación Celular , Cuello del Útero/metabolismo , Eritropoyetina/sangre , Displasia del Cuello del Útero/sangre , Cervicitis Uterina/sangre , Adolescente , Adulto , Cuello del Útero/patología , Enfermedad Crónica , Epitelio/metabolismo , Epitelio/patología , Femenino , Humanos , Persona de Mediana Edad , Displasia del Cuello del Útero/patologíaRESUMEN
In cervical secretions of healthy non-pregnant women of the reproductive age high concentrations of ant-inflammatory cytokines, greatly exceeding those in blood serum, were detected. During pregnancy the level of TNF-alpha in cervical secretions dropped. The inflammation of the uterus neck was accompanied by a drop in the levels of IL-alpha and IL-1beta and a rise in the level of IL-8 in cervical secretions in pregnant and non-pregnant women. Similar changes in the cytokine profile occurred also in the blood serum, but they were less pronounced and could be observed only in non-pregnant women. The threat of the interruption of pregnancy, developing simultaneously with cervicitis, was accompanied not only by changes in the levels of cytokines in cervical secretions, but also by a perceptible increase in the content of IL-1alpha in the blood serum.
Asunto(s)
Moco del Cuello Uterino/inmunología , Citocinas/análisis , Complicaciones Infecciosas del Embarazo/inmunología , Cervicitis Uterina/inmunología , Adulto , Moco del Cuello Uterino/química , Citocinas/sangre , Femenino , Humanos , Interleucina-1/análisis , Interleucina-1/sangre , Embarazo , Complicaciones Infecciosas del Embarazo/sangre , Nacimiento Prematuro/inmunología , Factor de Necrosis Tumoral alfa/análisis , Cervicitis Uterina/sangreRESUMEN
OBJECTIVE: To evaluate the usefulness of endocervical Gram stain smears in the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae in a female population attending a STD clinic. METHODS: 494 females attending a STD clinic and undergoing a speculum examination had endocervical specimens submitted for C trachomatis culture, direct fluorescent antibody testing (DFA), and N gonorrhoeae culture. Endocervical smears were also collected for Gram stain. The number of polymorphonuclear leucocytes (PMN) per high power field (HPF), presence of bacteria, yeast, red blood cells, and clue cells were recorded. Clinical signs of cervicitis were also documented. RESULTS: N gonorrhoeae was isolated from one subject who was co-infected with C trachomatis and no further analysis was done regarding N gonorrhoeae. Analysis was performed on 220 participants. The prevalence of C trachomatis infection was 13%. Of the Gram smears collected, 55% were inadequate owing to the presence of vaginal contamination. There were an equal number of C trachomatis isolates in patients with < or = 10 PMN/HPF (48%) and > 10 PMN/HPF (52%). Endocervical mucopus and erythema were statistically significant for the presence of C trachomatis (p < 0.001 and 0.02 respectively). The presence of any signs of cervicitis-that is, mucopus, friability, erythema, and ectropion together with > 10 PMN/HPF was statistically significant for the presence of C trachomatis. CONCLUSION: The use of endocervical Gram smear results together with clinical information can be used to identify high risk women for C trachomatis infection.
Asunto(s)
Infecciones por Chlamydia/diagnóstico , Chlamydia trachomatis/aislamiento & purificación , Violeta de Genciana , Fenazinas , Coloración y Etiquetado/métodos , Cervicitis Uterina/diagnóstico , Adolescente , Adulto , Infecciones por Chlamydia/sangre , Femenino , Técnica del Anticuerpo Fluorescente Directa , Gonorrea/sangre , Gonorrea/diagnóstico , Humanos , Recuento de Leucocitos , Neisseria gonorrhoeae/aislamiento & purificación , Neutrófilos , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Cervicitis Uterina/sangreRESUMEN
Human papillomaviruses (HPVs) are frequently present in anogenital lesions but little is known about their role in carcinogenesis. There are steroid hormone response elements in virus genomes that influence expression patterns of viral genes. Activity of the elements may contribute to development of neoplasia in case of hormone level anomalies. Our study was to determine whether the presence of HPV DNA in cervical smears correlates with abnormal levels, of steroid hormones in blood serum. One hundred women aged 40-62 participated in the tests and were divided into two groups: premenopausal and postmenopausal (45 and 55 individuals, respectively). Presence of HPV DNA in cervical smears was detected by PCR and Southern blot hybridisation. Progesterone and estradiol levels in blood serum were measured by radioimmunoassay. Our study showed a higher prevalence of HPV DNA in women with higher levels of progesterone in blood serum. A relationship between hormone level and HPV DNA prevalence should alert clinicians about using hormone contraceptives and hormone replacement therapy.
Asunto(s)
ADN Viral/análisis , Estradiol/sangre , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/sangre , Infecciones por Papillomavirus/diagnóstico , Progesterona/sangre , Infecciones Tumorales por Virus/sangre , Infecciones Tumorales por Virus/diagnóstico , Adulto , Secuencia de Bases , Southern Blotting , Estradiol/análisis , Femenino , Humanos , Persona de Mediana Edad , Datos de Secuencia Molecular , Estadificación de Neoplasias , Reacción en Cadena de la Polimerasa , Posmenopausia , Premenopausia , Progesterona/análisis , Radioinmunoensayo , Cervicitis Uterina/sangre , Cervicitis Uterina/diagnóstico , Cervicitis Uterina/virología , Frotis VaginalRESUMEN
OBJECTIVES: We investigated whether oxidative stress is associated with human uterine cervicitis and uterine myoma. DESIGN AND METHODS: We measured lipid peroxidation and antioxidant enzymes in plasma and erythrocytes of cervicitis patients and myoma patients in comparison with matched controls. Thiobarbituric acid-reactive substances (TBARS), a measure of lipid peroxidation, were determined in plasma; glutathione peroxidase (GSHPx) and catalase in erythrocytes; and superoxide dismutase (SOD) in both plasma and erythrocytes. RESULTS: We showed that plasma TBARS were significantly higher (p < 0.05) in both cervicitis patients and myoma patients than in controls. Plasma TBARS were significantly (and negatively) correlated with plasma and erythrocyte T-SOD activities in cervicitis patients only. Plasma T-SOD activity was significantly lower in both groups of patients than in controls whereas erythrocyte T-SOD activity was only significantly lower in myoma patients. The lowered plasma T-SOD activity in the cervicitis patients was attributed to decreased Mn-SOD activity whereas the lowered plasma T-SOD activity in myoma patients was attributed to decreased activities of both Cu,Zn-SOD and Mn-SOD. Erythrocyte GSHPx activity was 14% higher (p < 0.05) in cervicitis patients and 11% lower (p > 0.05) in myoma patients than in controls; catalase activity was 10% higher (p > 0.05) in cervicitis patients and 13% lower (p > 0.05) in myoma patients than in controls. Neither erythrocyte GSHPx nor catalase activity was significantly correlated with plasma TBARS. CONCLUSIONS: The elevated lipid peroxidation and disturbed antioxidant enzyme activities demonstrate the potential of oxidative injury in patients with uterine cervicitis and myoma.
Asunto(s)
Eritrocitos/enzimología , Glutatión Peroxidasa/sangre , Leiomioma/sangre , Peroxidación de Lípido , Superóxido Dismutasa/sangre , Cervicitis Uterina/sangre , Adulto , Femenino , Humanos , Leiomioma/enzimología , Persona de Mediana Edad , Estrés Oxidativo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Cervicitis Uterina/enzimología , Neoplasias Uterinas/sangre , Neoplasias Uterinas/enzimologíaRESUMEN
T(CD3+)-, B(CD19+)-lymphocytes and their subsets (CD4+, CD8+, CD3+ DR+, CD3- DR+) in peripheral blood of patients with CIN I, CIN II, CIN III and cancer in situ associated with HPV infection were evaluated. In peripheral blood of women with CIN II, CIN III and cancer in situ the number of T-lymphocytes which expressed CD3+ DR+ antigen decreased. In patients with CIN I, CIN III and cancer in situ the level of the CD4+ cells decreased; the level of the CD8+ cells increased. These patients had a lower CD4/CD8 ratio, the number of B cells being standard. The results may have important implications in the prognosis and immunotherapy of HPV infection.
Asunto(s)
Subgrupos Linfocitarios/inmunología , Papillomaviridae , Infecciones por Papillomavirus/inmunología , Infecciones Tumorales por Virus/inmunología , Displasia del Cuello del Útero/inmunología , Neoplasias del Cuello Uterino/inmunología , Cervicitis Uterina/inmunología , Adolescente , Adulto , Subgrupos de Linfocitos B/inmunología , Relación CD4-CD8 , Carcinoma in Situ/sangre , Carcinoma in Situ/inmunología , Estudios de Casos y Controles , Femenino , Humanos , Infecciones por Papillomavirus/sangre , Subgrupos de Linfocitos T/inmunología , Infecciones Tumorales por Virus/sangre , Neoplasias del Cuello Uterino/sangre , Cervicitis Uterina/sangre , Displasia del Cuello del Útero/sangreRESUMEN
An artificial glycoconjugate containing, as a ligand, the deacylated carbohydrate backbone of a recombinant Chlamydia-specific lipopolysaccharide was used as a solid-phase antigen in ELISA to measure antibodies against chlamydial LPS. The specificity and reproducibility of the assay was shown by using a panel of prototype monoclonal antibodies representing the spectrum of antibodies also occurring in patient sera. These mAbs recognized Chlamydia-specific epitopes [alpha 2-->8-linked disaccharide of 3-deoxy-D-manno-octulosonic acid (Kdo) or the trisaccharide alpha Kdo-(2-->8)-alpha Kdo-(2-->4)-alpha Kdo] or those shared between chlamydial and Re-type LPS (alpha Kdo, alpha 2-->4-linked Kdo disaccharide). The assay was used to measure IgG, IgA and IgM antibodies against chlamydial LPS in patients with genital or respiratory tract infections. In comparison to the results obtained with sera from blood donors, it became evident that both types of infection result in significant changes in the profile of LPS antibodies.