[Mineral content in freshwater fish in Shaanxi Province in 2021].

OBJECTIVE: To understand the mineral content of freshwater fish produced in Shaanxi Province and evaluate its related nutritional value. METHODS: According to the 2021 Shaanxi Provincial nutrition monitoring plan, the 9 mineral contents of 13 varieties of freshwater fish, produced in Shaanxi province, were determined by inductively coupled plasma atomic emission spectrometry. The nutritional evaluation of mineral elements was carried out by using the index of nutritional quality(INQ) method. Simultaneously, the correlation between 9 minerals and energy was analyzed by SPSS software. RESULTS: Among the 13 fish species, the contents of P and K were highest, with content ranges of 169-255 and 159-373 mg/100 g, respectively, followed by sodium, calcium, magnesium, iron, zinc. The contents of copper and manganese were lowest. The nutritional evaluation showed that the INQ values of P, K and Mg were than 1, the INQ value of P was highest, which was 4.57-8.72. Some fish have INQ values greater than 1 for calcium, iron, copper and zinc. The correlation between the nine minerals was not strong, as a whole. Only some elements have a correlation coefficient greater than 0.6, indicating that there was a synergistic accumulation effect or antagonistic effect in the fish body. CONCLUSION: The dominant mineral elements in different species of fish were different. However, most fish species can be used as high-quality food sources of phosphorus, potassium, magnesium, copper and zinc.

Health risks associated with mercury intake from rice and rice products.

The main route of human exposure to toxic contaminants is through the consumption of contaminated food. To protect against potential negative health effects of rice consumption, the concentration of total mercury (THg) in rice and rice products sold in Poland was determined, and a consumer risk assessment was carried out. In this study, the concentration of THg in selected types of rice and rice products was determined and assessed. The determinations were carried out using atomic absorption spectroscopy (AAS) in a DMA-1 mercury analyzer. Based on the estimated daily intake (EDI), target hazard quotient values (THQ), and tolerable weekly intake (TWI), the toxicological risk associated with the intake of total mercury and methylmercury (MeHg) from the products tested was also estimated. The analysis indicated that the highest Hg content was in rice cakes and the lowest in white rice and rice noodles. Total mercury content was found to be below the maximum permitted level. The estimated daily intake (EDI) and target hazard quotient (THQ) values for THg and MeHg in rice and rice products were lower than 1. These findings suggest that the food items examined pose no health risk.

Hg, Cd, and Pb in fish of the Moulouya River, Morocco, and human health risk.

Concentrations of toxic metals (Hg, Pb, and Cd) were determined in the muscles of Lepomis macrochirus, Barbus callensis, and Barbus nasus caught from the Moulouya River of Morocco by graphite furnace (for Pb and Cd) and cold vapour (for Hg) atomic absorption spectrometry, after acid digestion. Although the concentration of metals in some species was relatively high, no health risk has been identified in comparison to the maximum limits as set by the European Commission. The order of increasing concentrations was Cd < Hg < Pb. Calculated Target Hazard Quotients and Hazard Indices were below 1, indicating that the intake of metals via consumption of the muscles of both fish species does not represent a hazard to human health.

Development and validation of a microwave-assisted digestion technique as a rapid sample preparation method for the estimation of selenium in pharmaceutical dosage forms by ICP-OES.

Selenium is a class 2B element according to the International Council for Harmonization Q3D guidelines. Selenium sulfide is an anti-infective agent with antifungal and antibacterial properties used to treat dandruff and seborrheic dermatitis. The literature survey revealed that most of the analytical techniques to estimate selenium were time-consuming and/or required high skill levels. The process involved identifying the isotopes, selecting the measurement approach, and optimizing a typical microwave-aided digesting procedure. Ammonium hydrogen difluoride, water, and concentrated nitric acid were added to the samples. The confirmed microwave digestion program was a two-step program where in the initial step, the samples were ramped at 200°C for 20 min and held for 5 min. Later, samples were cooled and neutralized by boric acid, then ramped for 20 min to a temperature of 180°C and held for 10 min. Selenium was estimated at 196.090 nm by inductively coupled plasma optical emission spectroscopy (ICP-OES). System suitability was run before initiating analysis to ensure that system performance was consistent. Analytical validation parameters, such as the specificity of the method, were demonstrated at 196.090 nm, linearity was proven from 10 ppm to 150 ppm of selenium concentration, the detection limit was 1.28 ppm, and the limit of quantification was 3.89 ppm. Robustness was confirmed for small changes to ICP-OES operating conditions. The precision of the method demonstrated by analyzing the percentage relative standard deviation for six injections was found to be less than 2.0%. Accuracy was confirmed from 10 ppm to 150 ppm, and all the samples were observed to be within the range of 95%-105%. A common microwave-assisted digestion technique was developed and validated as well. The precision, specificity, linearity, accuracy, and robustness of the method for estimating selenium in selenium sulfide drug substances and various pharmaceutical dosage forms were demonstrated. This newly developed microwave-assisted digestion technique has optimum sensitivity and is highly reproducible and time-saving than the existing methods This method can be applied to numerous matrices for a finished dosage of selenium sulfide formulations.

Analysis of heavy metals and minerals in edible vegetable oils produced and marketed in Gondar City, Northwest Ethiopia.

INTRODUCTION: Nowadays, food safety is regarded as one of the most critical global public health issues. Edible oil, a key ingredient in food processing, is widely used and consumed in every Ethiopian household. However, its safety is often overlooked. Currently, edible oil is produced in Ethiopia from small-scale operations to large industrial levels, as well as imported from other countries. OBJECTIVE: This study aimed to determine the levels of heavy metals and essential minerals in edible vegetable oils produced and marketed in Gondar City, Northwest Ethiopia. METHODS: A laboratory-based cross-sectional study was conducted from May to July 2021 in Gondar City. Seventeen edible oil samples were collected using simple random sampling techniques. Heavy metal content was determined using an atomic absorption spectrophotometer with the standard procedures and techniques after microwave digestion. The efficiency and validity of the method used were evaluated by determining the limit of detection (LOD), the limit of quantification (LOQ), accuracy, and precision. The collected data were entered into Microsoft Excel and transported to Stata for analysis. RESULT: A total of seventeen vegetable oil samples were analyzed. The accuracy of the method was evaluated by recovery studies, which ranged from 81 to 115%, and the relative standard deviations were found to be below 15%. The concentrations of Zn, Cu, Fe, Cd, and Pb were in the range of 0.07 to 0.8 mg/l, 0.002 to 0.06 mg/l, 0.01 to 0.8 mg/l, 0.08 to 0.18 mg/l, and 0.003 to 0.27 mg/l, respectively. In general, the lead and cadmium content was higher than other metals in some of the investigated edible vegetable oils. Most values fell within the permissible quality limits for edibility as prescribed by the World Health Organization (WHO) and the National Agency for Food and Drug Administration and Control (NAFDAC). However, the levels of Pb and Cd exceeded the reference levels in some locally produced vegetable oils. CONCLUSION: To address the exceeded levels of heavy metals, it is imperative to implement more careful handling, processing of raw materials, and filtering practices. Producers and marketers should take the necessary precautions to prevent contamination. Strict regulatory control from responsible bodies and stakeholders is recommended to ensure the safety and metal contents of vegetable oils originating from the study area.

Insights into the Versatility of Using Atomic Absorption Spectrometry in Antibacterial Research.

The ongoing development of bacterial resistance to antibiotics is a global challenge. Research in that field is thus necessary. Analytical techniques are required for such a purpose. From this perspective, the focus was on atomic absorption spectrometry (AAS). Although it is old, AAS often offers unexpected potential. Of course, this should be exploited. The aim was therefore to demonstrate the versatility of the technique in antibacterial research. This is illustrated by various examples of its practical application. AAS can be used, for example, to confirm the identity of antibacterial compounds, for purity controls, or to quantify the antibiotics in pharmaceutical preparations. The latter allowed analysis without laborious sample preparation and without interference from other excipients. In addition, AAS can help elucidate the mode of action or resistance mechanisms. In this context, quantifying the accumulation of the antibiotic drug in the cell of (resistant) bacteria appears to play an important role. The general application of AAS is not limited to metal-containing drugs, but also enables the determination of some organic chemical antibiotics. Altogether, this perspective presents a range of applications for AAS in antibacterial research, intending to raise awareness of the method and may thus contribute to the fight against resistance.

Application of soil magnetometry and geochemical methods to investigate soil contamination with antimony.

The aim of the study was an assessment of the pollution level and identification of the antimony sources in soils in areas subjected to industrial anthropopressure from: transport, metallurgy and electrical waste recycling. The combination of soil magnetometry, chemical analyzes using atomic spectrometry (ICP-OES and ICP-MS), Sb fractionation analysis, statistical analysis (Pearson's correlation matrix, factor analysis) as well as Geoaccumulation Index, Pollution Load Index, and Sb/As factor allowed not only the assessment of soil contamination degree, but also comprehensive identification of different Sb sources. The results indicate that the soil in the vicinity of the studied objects was characterized by high values of magnetic susceptibility and thus, high contents of potentially toxic elements. The most polluted area was in the vicinity of electrical waste processing plants. Research has shown that the impact of road traffic and wearing off brake blocks, i.e. traffic anthropopression in general, has little effect on the surrounding soil in terms of antimony content. Large amounts of Pb, Zn, As and Cd were found in the soil collected in the vicinity of the heap after the processing of zinc-lead ores, the average antimony (11.31 mg kg-1) content was lower in the vicinity of the heap than in the area around the electrical and electronic waste processing plant, but still very high. Antimony in the studied soils was demobilized and associated mainly with the residual fraction.

Evaluating the quality of sediments in streams draining contrasting land-use areas in Osogbo metropolis, southwestern Nigeria.

The attendant effects of urbanization on the environment and human health are evaluable by measuring the potentially harmful element (PHE) concentrations in environmental media such as stream sediments. To evaluate the effect of urbanization in Osogbo Metropolis, the quality of stream sediments from a densely-populated area with commercial/industrial activities was contrasted with sediments from a sparsely-populated area with minimal anthropogenic input.Forty samples were obtained: 29 from Okoko stream draining a Residential/Commercial Area (RCA, n = 14) and an Industrial Area (IA, n = 15), and 11 from Omu stream draining a sparsely-populated area (SPA). The samples were air-dried, sieved to < 75 micron fraction, and analysed for PHEs using inductively-coupled plasma atomic emission spectrometry (ICP-AES). Index of geoaccumulation (Igeo), pollution index (PI), ecological risk factor (Er) and index (ERI) were used for assessment. Inter-elemental relationships and source identification were done using Pearson's correlation matrix and principal component analysis (PCA).PHE concentrations in the stream sediments were RCA: Zn > Pb > Cu > Cr > Sr > Ni > Co, IA: Zn > Cr > Ni > Co > Pb > Cu > Sr and SPA: Zn > Co > Cr > Cu > Sr > Ni > Pb. Igeo calculations revealed moderate-heavy contamination of Cu, Pb and Zn in parts of RCA, moderate-heavy contamination of Zn in IA while SPA had moderate contamination of Co and Zn. PI values revealed that stream sediments of RCA are extremely polluted, while those of IA and SPA are moderately and slightly polluted, respectively.The pollution of the stream sediments in RCA and IA is adduced to anthropogenic activities like vehicular traffic, automobile repairs/painting, blacksmithing/welding and metal scraping. In SPA however, the contamination resulted from the application of herbicides/fertilizers for agricultural purposes.

Recent Advances in the Determination of Major and Trace Elements in Plants Using Inductively Coupled Plasma Optical Emission Spectrometry.

Interest in measuring major and trace elements in plants has increased in recent years because of growing concerns about the elements' contribution to daily intakes or the health risks posed by ingesting vegetables contaminated by potentially toxic elements. The recent advances in using inductively coupled plasma atomic emission spectrometry (ICP-OES) to measure major and trace elements in plant samples are reviewed in the present work. The sample preparation before instrumental determination and the main advantages and limitations of ICP-OES are described. New trends in element extraction in liquid solutions using fewer toxic solvents and microextractions are observed in recently published literature. Even though ICP-OES is a well-established and routine technique, recent innovations to increase its performance have been found. Validated methods are needed to ensure the obtaining of reliable results. Much research has focused on assessing principal figures of merit, such as limits of detection, quantification, selectivity, working ranges, precision in terms of repeatability and reproducibility, and accuracy through spiked samples or certified reference materials analysis. According to the published literature, the ICP-OES technique, 50 years after the release of the first commercially available equipment, remains a powerful and highly recommended tool for element determination on a wide range of concentrations.

Specific of accumulation of manganese in organs and tissues of Hereford cattle.

The elemental status of cattle is one of the important factors, which determine its growth, fertility, fetal development, meat and dairy production, etc. Therefore, the study of content of different elements in cattle organs and tissues and its correlation with cattle characteristics and diet is urgent task. It is also important to develop intravital and low-invasive methods to analyze element content in cattle to regulate its diet during lifetime. In the present work, we have studied the content and distribution of manganese in Hereford cattle from an ecologically clean zone of Western Siberia (Russia). 252 samples were taken from 31 bulls aged 15-18 months. They were collected from various livestock farms in the region and analyzed using atomic absorption spectrophotometry (organs and muscle tissue) and inductively coupled plasma atomic emission spectrometry (hair). The median values of manganese concentration obtained in natural moisture for hair, heart, kidneys, liver, lungs, muscles, spleen, testes, and brain were 25, 0.37, 1.0, 2.6, 0.4, 0.2, 0.4, 0.5, and 0.5 ppm. Accordingly, the concentration of manganese differs significantly in the organs and tissues of animals (H = 188.6, df = 8, p <0.0001). Statistically significant associations of manganese were revealed in pairs: liver-testis, hair-testis, spleen-testis, and heart-brain. The classification of organs and tissues of animals according to the level of content and variability of manganese is carried out. The concentration of manganese in the body is not uniform, most of all it is deposited in the hair and excretory organs of the liver and kidneys. In other organs and muscle tissues, the distribution of manganese is more even and is in the range of 0.2-0.5 ppm. The resulting ranges can be used as a guideline for Hereford cattle bred in Western Siberia.

Assessment of human health risk concerning edible plants contamination with toxic elements around functional and derelict mines.

Edible plants samples were analysed for non-carcinogenic and carcinogenic human health risks. The elements nickel (Ni), cadmium (Cd), arsenic (As), lead (Pb), chromium (Cr) and mercury (Hg) analysed using atomic absorption spectrophotometer (AAS). The recovery, limit of detection (LOD) and limit of quantification (LOQ) ranged from 75 to 89 %, 0.001-0.003 and 0.003-0.01, respectively. The mean value of Hg (0.34 mg/kg) exceeded the limit of 0.05 mg/kg recommended by World Health Organization (WHO). The estimated daily intake (EDI) of Cd in adults ranged from 7.93 × 10-7 to 1.43 × 10-4 and that of Hg from 0.07 to 1.27 and Cd (0.08 × 10-4) in children. These are below the oral reference doses (RfD). Hazard Quotient (HQ) of Hg in children was 1.92. The elements Hg and As obtained the highest total hazard (THI) index of 2.02 in mango1 and oil palm. Arsenic possessed the highest cancer risk of 4.5 × 10-4 in children and 1.9 × 10-4 in adults. Cancer risk (CR) ranged from low to moderate (10-6-10-4), which is below the limit of 10-3. The total carcinogenic risk (TCR) of the edible plants were above the limit of 10-6. The study identified minimal As and Hg pollution and carcinogenic risks in edible plants.

Heavy metal pollution indices in soil and plants within the vicinity of the Gosa Dumpsite in Abuja, Nigeria.

Heavy metal contamination in the soil and phytoremediation potential of the plants cultivated around the Gosa dumpsite were evaluated using pollution indices. The concentrations of heavy metals in the soil and plant samples were determined using an atomic absorption spectrophotometer (Agilent 280FS AA). The mean heavy metal contents in the upper and lower soil layers ranged from 0.37 to 1662.61 mg/kg and 0.32 to 1608.61 mg/kg, respectively, in ascending order of Cd < Cr < Cu < Ni < Pb < Co < Zn < Fe. The results revealed a steady depthwise decrease in heavy metal contents from the upper to lower soil layers. Co, Pb, Zn and Fe were introduced through geogenic and anthropogenic pathways, while Cr, Ni, Cu and Cd were derived mainly from anthropogenic sources. The mean soil enrichment in the heavy metals ranged from 0.96 to 237.04 in the ascending order of Fe > Co > Pb > Zn > Cu > Cd > Cr > Ni. The soil was moderately polluted with Co, Cu, Pb, Zn, Fe and Cd but heavily polluted with Cr and Ni. The results revealed that 37.5% of the sites studied had pollution load indices greater than 1.0, indicating gradual deterioration in overall soil quality. The concentrations of Pb, Cd and Fe exceeded the recommended limits for the five plant species assessed. The transfer factor (TF) values of okra plant 1 (0.7536), water hyacinth (1.3768), and Amaranthus hybridus (0.9783) indicated excellent Cd phytoremediation potential. Okra Plant, water hyacinth and Amaranthus hybridus had excellent potential for phytoremediation of Cu, Fe and Pb, respectively. The study area was strongly enriched in Fe, Cd, Cr, and Ni, suggesting some degree of soil pollution, while the plants demonstrated an excellent capacity to accumulate Cd, Cu, Fe and Pb. This dumpsite should be adequately monitored while proper remediation measures are adopted by government authorities.

Bismuth Film along with dsDNA-Modified Electrode Surfaces as Promising (bio)Sensors in the Analysis of Heavy Metals in Soils.

Heavy metals constitute pollutants that are particularly common in air, water, and soil. They are present in both urban and rural environments, on land, and in marine ecosystems, where they cause serious environmental problems since they do not degrade easily, remain almost unchanged for long periods, and bioaccumulate. The detection and especially the quantification of metals require a systematic process. Regular monitoring is necessary because of seasonal variations in metal levels. Consequently, there is a significant need for rapid and low-cost metal determination methods. In this study, we compare and analytically validate absorption spectrometry with a sensitive voltammetric method, which uses a bismuth film-plated electrode surface and applies stripping voltammetry. Atomic absorption spectroscopy (AAS) represents a well-established analytical technique, while the applicability of anodic stripping voltammetry (ASV) in complicated sample matrices such as soil samples is currently unknown. This sample-handling challenge is investigated in the present study. The results show that the AAS and ASV methods were satisfactorily correlated and showed that the metal concentration in soils was lower than the limit values but with an increasing trend. Therefore, continuous monitoring of metal levels in the urban complex of a city is necessary and a matter of great importance. The limits of detection of cadmium (Cd) were lower when using the stripping voltammetry (SWASV) graphite furnace technique compared with those obtained with AAS when using the graphite furnace. However, when using flame atomic absorption spectroscopy (flame-AAS), the measurements tended to overestimate the concentration of Cd compared with the values found using SWASV. This highlights the differences in sensitivity and accuracy between these analytical methods for detecting Cd. The SWASV method has the advantage of being cheaper and faster, enabling the simultaneous determination of heavy elements across the range of concentrations that these elements can occur in Mediterranean soils. Additionally, a dsDNA biosensor is suggested for the discrimination of Cu(I) along with Cu(II) based on the oxidation peak of guanine, and adenine residues can be applied in the redox speciation analysis of copper in soil, which represents an issue of great importance.

Characteristics of minerals and oxide compounds in sediment collected from blood cockle culture areas at Bandon Bay, Thailand.

Bandon Bay is a very fertile bay for coastal aquaculture, especially for blood cockles (Anadara granosa). Its structural pattern supports the flow of nutrients which directly sent from many rivers resulted the high production capacity of blood cockle at the top level in the country. Besides organic compounds present in sediment, inorganic substances are essential for growth, survival and shell development of blood cockles. A comparative study of minerals and oxide compounds which accumulated in the sediments at eight stations around the cockle culture area was conducted. These stations are located along the estuaries at Tha Thong, Tha Chang, Phum Riang, and Tapi. The proportion of oxide compounds were determinedusing X-Ray Fluorescence (XRF) technique and minerals were analyzed by Atomic Absorption Spectroscopy (AAS). Results showed that sediment characteristics, oxide composition and the amount of minerals among the stations are different from each other. The sediments of the eastern and the western coasts were characterized as crumble clay and muddy sand, respectively. Twelve types of oxide compounds, namely SiO2, Al2O3, Fe2O3, K2O, Cl, MgO, Na2O, SO3, CaO, TiO2, MnO, P2O5 were found in various quantities, with SiO2, Al2O3, and Fe2O3 were the fundamental minerals ranging from 85.64-90.82%. Tha Thong estuary in the east coast showed highly significant quantities (P<0.05) of potassium, calcium and manganese compared to the other estuaries.

Heavy metal profile with health risk peculiarities in Enugu State and their long-term challenges in drinking water.

Health authorities are particularly concerned about water security in Enugu, southeast Nigeria and heavy metal (HM) pollution. The HM profiles of 51 samples collected from 17 different commercial bottled water brands in Enugu were examined using an flame atomic absorption spectroscopy. Cd, Cr, Cu, Pb, Ni, and Zn had mean values of 0.15 ± 0.03, 0.03 ± 0.02, 0.16 ± 0.03, 0.13 ± 0.02, and 0.02 ± 0.01 mg/L, respectively. The highest levels of Pb2+ were 0.27 mg/L in Exalté, Ni2+ 0.26 mg/L in Jasmine, Cd2+ 0.36 mg/L in Ezbon, Cr3+ 0.07 mg/L in Trinity, Cu2+ 0.04 mg/L in Bigi, and Zn2+ 0.02 mg/L in Aquarapha. The amounts of Cr, Cu, and Zn were below the allowable limits; nevertheless, the Pb content in eight bottled water samples exceeded both the Nigerian and World Health Organization (WHO)/U.S. Environmental Protection Agency (USEPA) permissible limits. The Cd2+ and Ni2+ levels in the 11th and 4th bottled water samples were above the WHO/USEPA-approved limits. Statistical evaluation revealed significant differences in the amounts of HM ions in the samples (p < 0.05). The findings indicated that concentration levels of Cd2+ Ni2+, and Pb2+ pose a public health concern that needs to be addressed due to potential risk to consumer health.

Inhibition of incipient caries lesion progression by different fluoridated varnishes.

The aim of this in vitro study was to evaluate the potential of different fluoridated varnishes to inhibit the progression of incipient caries lesions after cariogenic challenge. Seventy-five enamel specimens of bovine teeth were prepared and selected based on the initial surface microhardness (SMH). The specimens were first subjected to artificial demineralization (in buffer solution) after which SMH was re-analyzed (SM1). They were then randomly assigned to five experimental groups: 1- CONTROL (pH cycling), 2 - MI VAR (MI Varnish with RECALDENTTM - CPP-ACP), 3 - PROFL (Profluorid®), 4 - CLIN (ClinproTM White Varnish with TCP), and 5 - DUR (Duraphat®) (n=15). The varnishes were applied in a thin layer and the specimens were then subjected to pH cycling for eight days. The SMH and cross-sectional microhardness (CSMH) were then analyzed (SM2). The fluoride and calcium ion concentrations in the solution were analyzed by the indirect method and atomic absorption spectrophotometry, respectively. Data were statistically analyzed by Student's t-test, ANOVA/Tukey-Kramer, or Kruskall-Wallis/Dunn tests for individual comparisons (p˂0.05). All varnishes led to significantly higher surface and subsurface remineralization compared with the control group but did not differ from each other. The varnishes with the highest fluoride release were: PROFL and CLIN, followed by MI VAR and DUR. The varnishes with significantly higher release of calcium were: DUR, CLIN, and PROFL. In conclusion, all commercial fluoridated varnishes tested have good potential to inhibit the progression of demineralization, regardless of the ion release mechanisms.

Mercury in four common fishes sold in Algeria and associated humans risk.

This study aimed to analyse the concentrations of mercury in fish samples available in the Algerian market and evaluate the potential health risks associated with their consumption. A total of 135 fish samples, representing the species Sardina pilchardus, Merluccius merluccius, Sparus aurata and Auxis rochie, were collected and analysed. Mercury levels were determined using thermal decomposition amalgamation Atomic Absorption Spectrophotometry. Health risks were evaluated by calculating hazard quotients. The overall mean concentration was 0.19 ± 0.11 mg/kg across all species. Individual species concentrations were recorded as 0.17 ± 0.08 mg/kg for Sardina pilchardus, 0.26 ± 0.19 mg/kg for Merluccius merluccius, 0.27 ± 0.18 mg/kg for Sparus aurata and 0.23 ± 0.13 mg/kg for Auxis rochei. Hazard quotients were below 1, indicating low health risk for fish consumers. Nevertheless, it is recommended to conduct periodic monitoring of heavy metal levels in fish, coupled with ongoing risk assessments, to ensure continued consumer protection.

Interaction of carbonic anhydrase I released from red blood cells with human plasma in vitro.

Red blood cells (RBCs) constitute ∼50% of the bloodstream and represent an important target for environmental pollutants and bacterial/viral infections, which can result in their rupture. In addition, diseases such as sickle cell anaemia and paroxysmal nocturnal haemoglobinuria can also result in the rupture of RBCs, which can be potentially life-threatening. With regard to the release of cytosolic metalloproteins from RBCs into the blood-organ system, the biochemical fate of haemoglobin is rather well understood, while comparatively little is known about another highly abundant Zn-metalloprotein, carbonic anhydrase (CA I). To gain insight into the interaction of CA I with human blood plasma constituents, we have employed a metallomics tool comprised of size-exclusion chromatography (SEC) coupled online with an inductively coupled plasma atomic emission spectrometer (ICP-AES), which allows to simultaneously observe all Cu, Fe, and Zn-metalloproteins. After the addition of CA I to human blood plasma incubated at 37°C, the SEC-ICP-AES analysis using phosphate buffered saline (pH 7.4) after 5 min, 1 h, and 2 h revealed that CA I eluted after all endogenous Zn-metalloproteins in the 30 kDa range. Matrix-assisted laser desorption-time of flight mass spectrometry analysis of the collected Zn-peak confirmed that CA I eluted from the column intact. Our in vitro results suggest that CA I released from RBCs to plasma remains free and may be actively involved in health-relevant adverse processes that unfold at the bloodstream-endothelial interface, including atherosclerosis and vision loss.

The determination of thallium in the environment: A review of conventional and advanced techniques and applications.

Thallium (Tl) is a potential toxicity element that poses significant ecological and environmental risks. Recently, a substantial amount of Tl has been released into the environment through natural and human activities, which attracts increasing attention. The determination of this hazardous and trace element is crucial for controlling its pollution. This article summarizes the advancement and progress in optimizing Tl detection techniques, including atomic absorption spectroscopy (AAS), voltammetry, inductively coupled plasma (ICP)-based methods, spectrophotometry, and X-ray-based methods. Additionally, it introduces sampling and pretreatment methods such as diffusive gradients in thin films (DGT), liquid-liquid extraction, solid phase extraction, and cloud point extraction. Among these techniques, ICP-mass spectrometry (MS) is the preferred choice for Tl detection due to its high precision in determining Tl as well as its species and isotopic composition. Meanwhile, some new materials and agents are employed in detection. The application of novel work electrode materials and chromogenic agents is discussed. Emphasis is placed on reducing solvent consumption and utilizing pretreatment techniques such as ultrasound-assisted processes and functionalized magnetic particles. Most detection is performed in aqueous matrices, while X-ray-based methods applied to solid phases are summarized which provide non-destructive analysis. This work improves the understanding of Tl determination technology while serving as a valuable resource for researchers seeking appropriate analytical techniques.

Understanding the maturity of coconut water through 1H NMR profiling and MPAES analyses.

This study investigated the relationship between coconut maturity stages and the sugar, amino acid, and mineral profiles of coconut water (CW). Metabolite profiles were analysed using 1H NMR, covering glucose (G), fructose (F), sucrose (S), reducing sugars (RS), total sugars (TS), amino acids, and organic acids. Mineral composition was measured using Microwave Plasma Atomic Emission Spectroscopy (MPAES). The results revealed distinct metabolite and mineral profiles across different maturity stages. Immature CW had high G/F and RS/TS ratios but low S/G ratios. Conversely, mature CW showed decreased G/F and RS/TS ratios but an increase in S/G. Mineral analysis revealed potassium as the predominant mineral in CW, peaking in the youngest stage and declining with maturity. Sodium, magnesium, and calcium showed a similar pattern, with higher concentrations in early than in later stages. The study identifies the age of 9-10 months as optimal stages for selecting tender coconut water.