RESUMEN
An extensive phytochemical investigation on the EtOAc-soluble fraction of the 90% MeOH extract from the twigs and needles of the 'vulnerable' Chinese endemic conifer Tsuga forrestii (Forrest's hemlock) led to the isolation and characterization of 50 structurally diverse diterpenoids, including 15 unreported C-18 carboxylated ones (tsugaforrestiacids A-O, 1-15, resp.). Among them, compounds 1-7 are abieten-18-oic acids, compound 8 is an abieten-18-succinate, and compounds 10-12 are podocarpen-18-oic acids, whereas compounds 13-15 are pimarane-type, isopimarane-type, and totarane-type diterpenoid acids, respectively. Their structures and absolute configurations were determined by a combination of spectroscopic methods, GIAO NMR calculations and DP4+ probability analyses, electronic circular dichroism (ECD) data, and single crystal X-ray diffraction analyses. All the isolates were evaluated for their inhibitory activities against the ATP-citrate lyase (ACL), a key enzyme in cellular metabolism. Tsugaforrestiacids E (5) and H (8) were found to have significant inhibitory effects against ACL, with IC50 values of 5.3 and 6.2 µM, respectively. The interactions of the bioactive molecules with the ACL enzyme were examined by molecular docking studies. The isolated diterpenoids also provide chemotaxonomic evidence to support the delimitation of Tsuga from its closest sister group (Nothotsuga). The above findings highlight the importance of protecting plant species with unique and diverse secondary metabolites, which may be potential sources of new therapeutic agents for the treating ACL-associated diseases.
Asunto(s)
ATP Citrato (pro-S)-Liasa , Diterpenos , Fitoquímicos , Diterpenos/química , Diterpenos/farmacología , Diterpenos/aislamiento & purificación , ATP Citrato (pro-S)-Liasa/antagonistas & inhibidores , ATP Citrato (pro-S)-Liasa/metabolismo , Fitoquímicos/farmacología , Fitoquímicos/química , Fitoquímicos/aislamiento & purificación , China , Estructura Molecular , Inhibidores Enzimáticos/farmacología , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/aislamiento & purificación , Especies en Peligro de Extinción , Simulación del Acoplamiento Molecular , Relación Estructura-Actividad , Hojas de la Planta/químicaRESUMEN
BACKGROUND: In Chinese Pharmacopeia, Picrasma quassioides (PQ) stems and leaves are recorded as Kumu with antimicrobial, anti-cancer, anti-parasitic effects, etc. However, thick stems are predominantly utilized as medicine in many Asian countries, with leaves rarely used. By now, the phytochemistry and bioactivity of PQ leaves are not well investigated. METHODS: An Orbitrap Elite mass spectrometer was employed to comprehensively investigate PQ stems and leaves sourced from 7 different locations. Additionally, their bioactivities were evaluated against 5 fungi, 6 Gram-positive bacteria and 9 Gram-negative bacteria, a tumor cell line (A549), a non-tumor cell line (WI-26 VA4) and N2 wild-type Caenorhabditis elegans. RESULTS: Bioassay results demonstrated the efficacy of both leaves and stems against tumor cells, several bacteria and fungi, while only leaves exhibited anthelmintic activity against C. elegans. A total of 181 compounds were identified from PQ stems and leaves, including 43 ß-carbolines, 20 bis ß-carbolines, 8 canthinone alkaloids, 56 quassinoids, 12 triterpenoids, 13 terpenoid derivatives, 11 flavonoids, 7 coumarins, and 11 phenolic derivatives, from which 10 compounds were identified as indicator components for quality evaluation. Most alkaloids and triterpenoids were concentrated in PQ stems, while leaves exhibited higher levels of quassinoids and other carbohydrate (CHO) components. CONCLUSION: PQ leaves exhibit distinct chemical profiles and bioactivity with the stems, suggesting their suitability for medicinal purposes. So far, the antibacterial, antifungal, and anthelmintic activities of PQ leaves were first reported here, and considering PQ sustainability, the abundant leaves are recommended for increased utilization, particularly for their rich content of PQ quassinoids.
Asunto(s)
Caenorhabditis elegans , Fitoquímicos , Picrasma , Hojas de la Planta , Tallos de la Planta , Hojas de la Planta/química , Picrasma/química , Animales , Tallos de la Planta/química , Caenorhabditis elegans/efectos de los fármacos , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Humanos , Línea Celular Tumoral , Estructura Molecular , Antineoplásicos Fitogénicos/farmacología , Alcaloides/farmacología , Cuassinas/farmacología , Cuassinas/química , Cuassinas/aislamiento & purificación , Antihelmínticos/farmacología , Antihelmínticos/química , Hongos/efectos de los fármacos , Flavonoides/farmacología , Flavonoides/análisisRESUMEN
Morus alba L. is a plant with a long history of dietary and medicinal uses. We hypothesized that M. alba possesses a significant biological potential. In that sense, we aimed to generate the chemical, antimicrobial, toxicological, and molecular profile of M. alba leaf and fruit extracts. Our results showed that extracts were rich in vitamin C, phenols, and flavonoids, with quercetin and pterostilbene concentrated in the leaf, while fisetin, hesperidin, resveratrol, and luteolin were detected in fruit. Extracts exhibited antimicrobial activity against all tested bacteria, including multidrug-resistant strains. The widest inhibition zones were in Staphylococcus aureus ATCC 33591. The values of the minimum inhibitory concentration ranged from 15.62 µg/ml in Enterococcus faecalis to 500 µg/ml in several bacteria. Minimum bactericidal concentration ranged from 31.25 µg/ml to 1000 µg/ml. Extracts impacted the biofilm formation in a concentration-dependent and species-specific manner. A significant difference in the frequency of nucleoplasmic bridges between the methanolic extract of fruit (0.5 µg/ml, 1 µg/ml, 2 µg/ml), as well as for the frequency of micronuclei between ethanolic extract of leaf (2 µg/ml) and the control group was observed. Molecular docking suggested that hesperidin possesses the highest binding affinity for multidrug efflux transporter AcrB and acyl-PBP2a from MRSA, as well as for the SARS-CoV-2 Mpro. This study, by complementing previous research in this field, gives new insights that could be of great value in obtaining a more comprehensive picture of the Morus alba L. bioactive potential, chemical composition, antimicrobial and toxicological features, as well as molecular profile.
Asunto(s)
Antibacterianos , Frutas , Pruebas de Sensibilidad Microbiana , Simulación del Acoplamiento Molecular , Morus , Fitoquímicos , Extractos Vegetales , Hojas de la Planta , Morus/química , Extractos Vegetales/farmacología , Extractos Vegetales/química , Hojas de la Planta/química , Antibacterianos/farmacología , Antibacterianos/química , Frutas/química , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Fitoquímicos/química , Biopelículas/efectos de los fármacos , Bacterias/efectos de los fármacos , Estructura Molecular , Flavonoides/farmacología , Flavonoides/químicaRESUMEN
Osage orange trees (Maclura pomifera (Raf.) C.K.Schneid.) are distributed worldwide, particularly in south-east states of the USA. They produce large quantities of strong yellow fruits, bigger than oranges, but these fruits are inedible, with an acid milky juice which is little consumed by birds and insects. Extracts prepared from Osage orange fruits (hedge apple) have revealed a range of pharmacological properties of interest in human and veterinary medicine. In addition, Osage orange extracts can be used in agriculture and aquaculture, and as dyeing agent for the textile industry. Extracts contain potent antioxidant compounds, notably the isoflavonoids pomiferin and auriculasin, together with other terpenoids and flavonoids. The structural characteristics and pharmacological properties of the major prenylated isoflavones isolated from M. pomifera are discussed here, with a focus on the two phenolic compounds osajin and warangalone, and the two catechol analogues pomiferin and auriculasin. The mechanisms at the origin of their potent antioxidant and anti-inflammatory effects are presented, notably inhibition of xanthine oxidase, phosphodiesterase 5A and kinases such as RKS2 and kRAS. Osajin and auriculasin display marked anticancer properties, owing to their ability to inhibit tumor cell proliferation, migration and tumor angiogenesis. Different molecular mechanisms are discussed, including osajincopper complexation and binding to quadruplex DNA. An overview of the mechanism of action of the prenylated isoflavones from Osage orange is presented, with the objective to promote their knowledge and to raise opportunities to better exploit the fruits of Osage orange, abundant but largely neglected at present.
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Antioxidantes , Frutas , Isoflavonas , Maclura , Frutas/química , Isoflavonas/farmacología , Isoflavonas/aislamiento & purificación , Isoflavonas/química , Maclura/química , Humanos , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Estructura Molecular , Extractos Vegetales/farmacología , Extractos Vegetales/química , Antiinflamatorios/farmacología , Antiinflamatorios/aislamiento & purificación , Prenilación , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Animales , Fenoles/farmacología , Fenoles/aislamiento & purificación , BenzopiranosRESUMEN
Herpetospermum pedunculosum seeds also known as Herpetospermum caudigerum Wall. is the mature seed of the Herpetospermum pedunculosum(Ser.) C. B. Clarke,Cucurbitaceae. Modern pharmacological studies have shown that H. pedunculosum has hepatoprotective, anti-inflammatory, anti-gout and antibacterial pharmacological activities. The biologically active chemical components include lignin compounds such as Herpetin, Herpetetrone, Herpetoriol and so on. The natural product displays considerable skeletal diversity and structural complexity, offering significant opportunities for novel drug discovery. Based on the multi-omics research strategy and the 'gene-protein-metabolite' research framework, the biosynthetic pathway of terpenoids and lignans in H. pedunculosum has has been elucidated at multiple levels. These approaches provide comprehensive genetic information for cloning and identification of pertinent enzyme genes. Furthermore, the application of multi-omics integrative approaches provides a scientific means to elucidate entire secondary metabolic pathways. We investigated the biosynthetic pathways of lignin and terpene components in H. pedunculosum and conducted bioinformatics analysis of the crucial enzyme genes involved in the biosynthetic process using genomic and transcriptomic data. We identified candidate genes for six key enzymes in the biosynthetic pathway. This review reports on the current literature on pharmacological investigations of H. pedunculosum, proposing its potential as an antidiabetic agent. Moreover, we conclude, for the first time, the identification of key enzyme genes potentially involved in the biosynthesis of active compounds in H. pedunculosum. This review provides a scientific foundation for the discovery of novel therapeutic agents from natural sources.
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Cucurbitaceae , Semillas , Terpenos , Semillas/química , Terpenos/farmacología , Cucurbitaceae/química , Lignina/química , Vías Biosintéticas , Lignanos/farmacología , Lignanos/biosíntesis , Lignanos/química , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Fitoquímicos/química , Genómica , MultiómicaRESUMEN
This study was designed to investigate chemical composition and biological activities of the Anthriscus cerefolium methanolic extract. Chemical characterization of the extracts was performed by LC-HRMS/MS analysis. Antimicrobial activities of the extract were investigated on six bacteria and eight fungi while antioxidant activity was assessed by six different assays. Anti-enzymatic activity of the methanolic extract was tested on five enzymes associated with therapy of neurodegenerative diseases and diabetes mellitus type 2. Cytotoxic properties of the extract were tested on human immortalized keratinocytes (HaCaT) and tumor cell lines (SiHa, MCF7, HepG2). Anti-inflammatory activity of the extract was assessed on bacteria mediated inflammation model using HaCaT cell line. Molecular docking studies of enzymes and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed. The results showed that the obtained extract was rich in phenolic compounds (a total of seventy-two were identified), with malonyl-1,4-O-dicaffeoylquinic acid and 3,5-O-dicaffeoylquinic acid dominating in the sample. The extract expressed antimicrobial, antioxidant, anti-enzymatic, cytotoxic and anti-inflammatory properties. The identified compounds demonstrated strong binding to the acetylcholinesterase (AChE) and to a lesser extent, to the butyrylcholinesterase (BChE), glucosidase, amylase, and modestly, to tyrosinase. KEGG pathway analysis has shown that the certain phenolic compounds may be related to anti-tumor, anti-inflammatory and anti-microbial activities of the extract. The data obtained suggest that phenolic compounds of the extract and their mixtures should be considered for future research as ingredients in pharmaceutical and nutraceutical formulations.
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Antiinflamatorios , Antioxidantes , Simulación del Acoplamiento Molecular , Extractos Vegetales , Humanos , Extractos Vegetales/farmacología , Extractos Vegetales/química , Antioxidantes/farmacología , Antiinflamatorios/farmacología , Línea Celular Tumoral , Antiinfecciosos/farmacología , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Fenoles/farmacologíaRESUMEN
Chemical investigation on the aqueous extract of Dendrobium aphyllum led to the isolation of thirty-one constituents with structures identified by analysis of the extensive spectroscopic data (1D/2D NMR, MS, UV, and ECD), including previously undescribed two bibenzyls, one furfural, and one phenolic acid, namely trigonopol D (1), trigonopol C (2), dendrofunan A (10), and 6-(4-hydroxy-3-methoxyphenyl)-3,6-dioxohexyl acetate (30), respectively, as well as twenty-seven known ones. Among them, there were one new natural product (11), seven compounds (6-7, 9, 12, 20, 28, 31) described from the genus Dendrobium for the first time, and fifteen compounds (8, 13-17, 19, 21-27, 29) isolated from D. aphyllum for the first time. Further, the antioxidant and anti-inflammatory potentials of fifteen compounds (4-5, 8, 11-12, 14-19, 22, 24, 26, and 29) with significant scavenging capacities against DPPH and hydroxyl radicals, and virtual docking activities inhibiting COX-2 and 5-LOX, respectively. Our study may draw the attention of medicinal plant taxonomists and supply potential quality markers for discrimination of D. aphyllum from other species in Dendrobium genus.
Asunto(s)
Antiinflamatorios , Antioxidantes , Bibencilos , Dendrobium , Fitoquímicos , Dendrobium/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Antiinflamatorios/farmacología , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/química , Estructura Molecular , Bibencilos/farmacología , Bibencilos/aislamiento & purificación , Bibencilos/química , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Fenantrenos/farmacología , Fenantrenos/aislamiento & purificación , Fenantrenos/química , Fenilpropionatos/aislamiento & purificación , Fenilpropionatos/farmacología , Fenilpropionatos/química , China , Animales , Ratones , Araquidonato 5-Lipooxigenasa/metabolismo , Simulación del Acoplamiento Molecular , Furanos/aislamiento & purificación , Furanos/farmacología , Furanos/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Ciclooxigenasa 2/metabolismoRESUMEN
Periodontitis is clinically characterized by destruction of the tooth support system and tooth loss. Porphyromonas gingivalis (Pg) plays a dominant role in periodontitis. Fractions and isolated compounds from an acetone-water extract of the roots of Limonium brasiliense (Lb) were tested in vitro for their anti-adhesive capacity against Pg on human KB buccal cells, influence on gingipains, the main virulence factors of Pg, and biofilm formation. Fractions EAF and FLB7 (50 µg/mL) reduced the bacterial adhesion of Pg to KB cells significantly (63 resp. 70%). The proanthocyanidin samarangenin A inhibited the adhesion (72%, 30 µM), samarangenin B (71%, 20 µM), and the flavan-3-ol epigallocatechin-3-O-gallate (79%, 30 µM). Fraction AQF, representing hydrophilic compounds, reduced the proteolytic activity of Arginin-specific gingipain (IC50 12.78 µg/mL). Fractions EAF and FLB7, characterized by lipohilic constituents, inhibited Arg-gingipain (IC50 3 µg/mL). On Lysine-specific gingipain, AQF has an IC50 15.89, EAF 14.15, and FLB7 6 µg/mL. The reduced bacterial adhesion is due to a strong interaction of proanthocyanidins with gingipains. AQF, EAF, and FLB7 significantly inhibited biofilm formation: IC50 11.34 (AQF), 11.66 (EAF), and 12.09 µg/mL (FLB7). In silico analysis indicated, that the polyphenols act against specific targets of Pg, not affecting mammalian cells. Therefore, Lb might be effective for prevention of periodontal disease by influencing virulence factors of Pg.
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Adhesinas Bacterianas , Adhesión Bacteriana , Biopelículas , Cisteína Endopeptidasas , Cisteína-Endopeptidasas Gingipaínas , Extractos Vegetales , Plumbaginaceae , Porphyromonas gingivalis , Factores de Virulencia , Biopelículas/efectos de los fármacos , Porphyromonas gingivalis/efectos de los fármacos , Humanos , Adhesinas Bacterianas/efectos de los fármacos , Adhesión Bacteriana/efectos de los fármacos , Extractos Vegetales/farmacología , Extractos Vegetales/química , Plumbaginaceae/química , Raíces de Plantas/química , Proantocianidinas/farmacología , Proantocianidinas/aislamiento & purificación , Células KB , Antibacterianos/farmacología , Antibacterianos/aislamiento & purificación , Antibacterianos/química , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificaciónRESUMEN
BACKGROUND: Myrsine (the family Primulaceae) contains flowering species. Pharmacologically, the plants of this genus belong to a list of medicinal plants that induce infectious and inflammatory treatments. There are no scientific publications that review phytochemistry and pharmacological activities. OBJECTIVE: The compilation and classification of phytochemicals, chromatographic information, essential oils, and pharmacological reviews are the ultimate aim. METHODS: References on phytochemical and pharmacological investigations of Myrsine species were collected from various sources, such as Google Scholar, PubMed, and Web of Science from the 1990s to present. The main keyword "Myrsine" was used alone or in combination with others to search for references. RESULTS: Chromatographic procedure of Myrsine extracts led to the purification of 134 compounds. Flavonoids, mono-phenols, saponins, quinones, megastigmanes, and lignans were the main phytochemical classes. Myrsine Volatile compounds are monoterpenoids, sesquiterpenoids, and aliphatic compounds. Myrsine constituents established a widespread panel of pharmacological activities, such as cytotoxicity, antioxidant, antimicrobial, anti-parasite, tyrosine inhibition, and hepatoprotection, especially anti-inflammation. Novel flavonoids myrsininones A-B are better than the standard triclosan against bacteria Staphylococcus warneri, S. mutan, S. sanguis, and Actinomyces naeslundii. M. seguinii aerial part ethanolic extract inhibited LPS (lipopolysaccharide)-stimulated inflammatory Raw 264.7 cells via Src/Syk/NF-κB (sarcoma kinase/spleen tyrosine kinase/ nuclear factor-kappa B) and IRAK-1/AP-1 (interleukin-1 receptor-associated kinase-1/activating protein-1) signaling inhibition. Generally, Myrsine plant extracts showed no toxicity. CONCLUSION: Myrsine constituents are good antimicrobial, antioxidative, and anti-inflammatory agents. However, the majority of earlier research focuses on the pharmacological analyses of M. africana. Thus, comprehensive findings for the remaining species are needed.
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Fitoquímicos , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Animales , Aceites Volátiles/farmacología , Aceites Volátiles/química , Plantas Medicinales/química , Extractos Vegetales/farmacología , Extractos Vegetales/química , Humanos , Estructura Molecular , Antiinflamatorios/farmacología , Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificaciónRESUMEN
Mondia whitei is an aromatic plant native to sub-Saharan Africa. This spice is commonly used in the treatment of various diseases, such as hypertension, diabetes, erectile dysfunction, and premature ejaculation. This review was undertaken to provide updated information on the botanical, phytochemical, pharmacological, and toxicological knowledge of this plant of high relevance to African populations. Moreover, its mechanism of action was described based on previous experimental studies. Data were compiled from various online databases such as PubMed, Google Scholar, Scopus, Science Direct, Web of Science, Springer link, Taylor and Francis, and SciFinder. Additionally, books, book chapters and proceedings were used as secondary sources. The chemical structures of phytocompounds were drawn using PubChem Sketcher program. M. whitei contains various phytocompounds, including reducing sugars, triterpenes, steroids, alkaloids, saponins, tannins, phenolics, hydrogen cyanide, carotenoid, oxalate and phytate. Moreover, para-pentylphenyl-benzoate, (-)-Loliolide, 5-chloropropacin, propacin, 2-hydroxy-4-methoxybenzaldehyde, isovanillin, 9-hexacosene, 2-hexen-1-ol, and heptacosane were isolated from this spice. M. whitei has several pharmacological benefits, including aphrodisiac, pro-fertile, pro-erectile, androgenic, antioxidant, antiparasitic, antimalarial, antibacterial, antiviral, antifungal, antiepileptic, anti-inflammatory, analgesic, antidepressant, antidiarrheal, hepatoprotective, antisickling, and anticancer activities. Toxicological studies showed an LD50 of above 5000 mg/Kg and no signs of toxicity after one week of oral treatment. The aphrodisiac effect of this spice is one of its main activities, supported by numerous experimental studies. Because M. whitei delays contractions of the bulbospongiosus muscles, its aphrodisiac effect could be mediated through the modulation of the spinal generator of ejaculation. This can justify its folkloric use in the treatment of premature ejaculation.
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Medicinas Tradicionales Africanas , Fitoquímicos , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Humanos , África del Sur del Sahara , Estructura Molecular , AnimalesRESUMEN
Phytochemical investigation on the extract of the seeds of Thevetia peruviana resulted in the isolation of six new cardiac glycosides, namely theveperosides A-F (1-6), including a rare 19-nor-cardenolide (1), together with seven known analogues (7-13). The chemical structures of these compounds were determined based on detailed spectroscopic analysis. The cytotoxic activities of 1-13 were evaluated against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, and their structure-activity relationships (SARs) were investigated. Compound 3 exhibited the significant cytotoxic effects with IC50 values ranging from 0.032 to 0.055 µΜ, which could induce HepG2 cells apoptosis in a dose-dependent manner.
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Antineoplásicos Fitogénicos , Glicósidos Cardíacos , Fitoquímicos , Semillas , Thevetia , Humanos , Glicósidos Cardíacos/farmacología , Glicósidos Cardíacos/aislamiento & purificación , Glicósidos Cardíacos/química , Semillas/química , Estructura Molecular , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Thevetia/química , Relación Estructura-Actividad , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Apoptosis/efectos de los fármacosRESUMEN
Two previously unreported lindenane sesquiterpene dimers (1 and 2) with a rare skeleton containing an oxaspiro[4.5]decane moiety were isolated from the roots of Chloranthus holostegius var. trichoneurus. Their structures were elucidated by HRESIMS, NMR, ECD, and NMR quantum chemical calculations, along with DP4+ probability analysis. In bioassay, compound 1 exhibited significant activity to reverse the multidrug resistance (MDR)in MCF-7/ADR cells, with an IC50 value of 4.4 µM. Further mechanistic studies revealed that compound 1 combined with doxorubicin could induce apoptosis of MCF-7/ADR cells and block the cell cycle in the G2/M phase. Mechanistically, compound 1 could inhibit the efflux function of P-glycoprotein (P-gp) using the zebrafish model. Finally, the enhanced chemotherapeutic effects of doxorubicin were further confirmed by in vivo zebrafish xenograft experiments.
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Apoptosis , Doxorrubicina , Resistencia a Múltiples Medicamentos , Resistencia a Antineoplásicos , Raíces de Plantas , Sesquiterpenos , Pez Cebra , Animales , Humanos , Estructura Molecular , Resistencia a Múltiples Medicamentos/efectos de los fármacos , Sesquiterpenos/farmacología , Sesquiterpenos/aislamiento & purificación , Doxorrubicina/farmacología , Resistencia a Antineoplásicos/efectos de los fármacos , Apoptosis/efectos de los fármacos , Raíces de Plantas/química , Células MCF-7 , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Magnoliopsida/química , ChinaRESUMEN
Chrysosplenium axillare Maxim. is used in traditional Tibetan medicine for the treatment of various human diseases, such as fever, headache, cholecystitis, acute icterohepatitis and acute liver necrosis. In this study, five new cucurbitane triterpenoid derivatives, chrysosaxillins A-E (1-5), along with three known structurally related compounds (6-8) have been isolated from whole herb of C. axillare. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR, HRESIMS, UV, IR, ECD and single-crystal X-ray diffraction. All isolates were evaluated for cytotoxic activities against four tumor cell lines including PC-3, A549, MCF-7, and HepG2. The results discovered that compound 1 possessed the most potent cytotoxicity against A549 cells with IC50 value of 0.05 µM, while compounds 2 and 4 have mild cytotoxicities against cells tested with IC50 values ranging from 8.78 to 41.72 µM. Our study suggests that C. axillare might serve as a valuable source of cucurbitane triterpenoids potentially useful for the development of new anti-tumor agents and support its use as a crop benefits to local economic.
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Antineoplásicos Fitogénicos , Fitoquímicos , Triterpenos , Humanos , Triterpenos/aislamiento & purificación , Triterpenos/farmacología , Triterpenos/química , Estructura Molecular , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Línea Celular Tumoral , GlicósidosRESUMEN
The classic Astragalus-Cassia twig drug pair has a long history of proven efficacy. However, a fewer studies on material basis of the Astragalus and Cassia twig decoction (ACD) was researched at present. The method of UPLC-Q-TOF-MS for classifying and identifying the main chemical components of ACD was established and the differences in composition between single decoction and co-decoction were compared by using HPLC-UV. The therapeutic role of ACD on type 2 diabetes (T2D) rats was investigated. Thirty-five compounds were resolved from the ACD. Fifteen compounds were deduced from the decoction of Astragalus, whereas nine compounds were identified from Cassia twig. Pairing of herbs make a significant effect on the chemical composition of herbal decoction. ACD can play a more obvious role in alleviating the symptoms of T2D rats, compared to the application of single herb.
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Planta del Astrágalo , Animales , Cromatografía Líquida de Alta Presión , Ratas , Planta del Astrágalo/química , Masculino , Ratas Sprague-Dawley , Cassia/química , Medicamentos Herbarios Chinos/química , Espectrometría de Masas , Estructura Molecular , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Tipo 2/tratamiento farmacológicoRESUMEN
A naturally occurring stilbene, resveratrol, shows promising effects in the treatment of malignant pleural mesothelioma (MPM) both as a single agent and in combination with chemotherapeutic drugs. To discover new anticancer agents targeting MPM, stilbene-targeted isolation was performed on the roots of Polygonum multiflorum Thunb., an herbal medicine rich in stilbene compounds. In this study, seven stilbene glycosides (1-7) were isolated, along with four non-stilbenes (8-11), of which compounds 4 and 9-11 have not previously been isolated from this species. Stiquinoside A (1) is a previously undescribed stilbene glycoside, and its structure was elucidated as (E)-2,3,5,4'-tetrahydroxystilbene 2-O-ß-d-quinovopyranoside based on 1D and 2D-NMR, HR-ESI-MS, and acid hydrolysis experiments. Compounds 1, 4, 6, and 8 significantly inhibit the growth of MPM cancer cells H2452. These results demonstrate the potential utility of stilbenes in new strategies for the treatment of MPM.
Asunto(s)
Antineoplásicos Fitogénicos , Fallopia multiflora , Mesotelioma Maligno , Raíces de Plantas , Estilbenos , Humanos , Estilbenos/farmacología , Estilbenos/aislamiento & purificación , Raíces de Plantas/química , Estructura Molecular , Línea Celular Tumoral , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Mesotelioma Maligno/tratamiento farmacológico , Fallopia multiflora/química , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Glicósidos/farmacología , Glicósidos/aislamiento & purificación , Mesotelioma/tratamiento farmacológico , Neoplasias Pulmonares/tratamiento farmacológico , ChinaRESUMEN
Pandan (Pandanus amaryllifolius Roxb.), a member of the Pandanaceae family, has been consumed as food and medicine since ancient times. The current paper provides an overview of the botanical profile, phytochemistry, pharmacology, and applications of P. amaryllifolius. Information regarding P. amaryllifolius was collected from online sources (using PubMed, Science Direct, Google Scholar, Web of Science, ACS, and CNKI) as well as traditional textbooks. Over 100 compounds have been identified, including its characteristic components 2-Acetyl-1-pyrroline and Pandanus alkaloids. Several therapeutic uses of P. amaryllifolius, such as antioxidant, hypoglycemic, antimicrobial, and antitumor activities, have been demonstrated in modern pharmacological studies. Additionally, it could be applied in various fields, including food, energy, material, and the environment. Continued research on P. amaryllifolius can contribute to the development of new drugs and therapies for various diseases. And further studies are needed to improve its utilization.
Asunto(s)
Pandanaceae , Fitoquímicos , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Estructura Molecular , Pandanaceae/química , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Antioxidantes/química , Humanos , Antiinfecciosos/farmacología , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Hipoglucemiantes/farmacología , Hipoglucemiantes/química , Alcaloides/farmacología , Alcaloides/aislamiento & purificación , Alcaloides/química , Extractos Vegetales/farmacología , Extractos Vegetales/químicaRESUMEN
PURPOSE: Natural plant raw materials, previously underestimated in therapeutics, are becoming the subject of research for new applications in medicine. In our research, the hydroalcoholic extract of Isatis tinctoria leaf, rich in flavonoid compounds such as vicenin-2 and quercetin, was examined as a potential antidiabetic and neuroprotective agent. METHODS: The effect of the extract and its main flavonoid compounds on protein glycation, alpha-glucosidase activity, and acetylcholinesterase activity was tested. In vitro, in the mouse hippocampal neuronal cell line and in vivo, using a mouse model, the safety of the extract was screened for. RESULTS: Our experiments demonstrated significant inhibition of protein glycation, alpha-glucosidase activity, acetylcholinesterase activity, and ß-amyloid aggregation by the extract, in a concentration-dependent manner. The extract had a strong reducing effect and did not exhibit cytotoxicity up to a concentration of 25 mg/mL. Intraperitoneal administration of the extract to mice did not have negative effects on body mass, locomotor activity, coordination, and liver cell integrity. CONCLUSIONS: Our research sheds new light on this raw material and deepens knowledge of its activity. This may result in the recognition of its therapeutic effects and even in its introduction in the modern treatment of diseases characterized by pathological changes associated with hyperglycemia, oxidation, and inflammation.
Asunto(s)
Hipoglucemiantes , Fármacos Neuroprotectores , Extractos Vegetales , Hojas de la Planta , Animales , Extractos Vegetales/farmacología , Ratones , Fármacos Neuroprotectores/farmacología , Fármacos Neuroprotectores/aislamiento & purificación , Hipoglucemiantes/farmacología , Hojas de la Planta/química , Masculino , Línea Celular , Péptidos beta-Amiloides , Flavonoides/farmacología , Flavonoides/aislamiento & purificación , Acetilcolinesterasa/metabolismo , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Inhibidores de Glicósido Hidrolasas/farmacología , Hipocampo/efectos de los fármacos , Neuronas/efectos de los fármacos , alfa-Glucosidasas/metabolismoRESUMEN
Five new compounds, including two sesquiterpenoid glycosides (1 and 2), two monoterpenoid glycosides (3 and 4), and a quinovose ester (5), together with four known compounds (6-9) were isolated from branches and leaves of Pittosporum pulchrum Gagnep. Their structures were established by 1D and 2D NMR, HR-ESI-MS, IR and UV spectral analyses. This is the first time to investigate the chemical constituents of P. pulchrum. Subsequently, the anti-inflammatory and antioxidant activities of different solvent fractions of ethanol extract and isolated compounds were evaluated. Dichloromethane and ethyl acetate fractions dramatically inhibited the production of NO in a concentration-dependent manner in LPS-induced RAW264.7 cells. Ethyl acetate and n-butanol fractions showed excellent DPPH radical scavenging activities with IC50 values of 24.31 µg/mL and 27.81 µg/mL, respectively. Compounds 7 and 8 might be potential natural antioxidants with IC50 values of 16.13 µM and 24.81 µM, respectively.
Asunto(s)
Antiinflamatorios , Antioxidantes , Fitoquímicos , Hojas de la Planta , Hojas de la Planta/química , Ratones , Células RAW 264.7 , Animales , Estructura Molecular , Antioxidantes/farmacología , Antioxidantes/aislamiento & purificación , Antioxidantes/química , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Antiinflamatorios/farmacología , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Glicósidos/química , Óxido Nítrico/metabolismo , Tallos de la Planta/química , Sesquiterpenos/aislamiento & purificación , Sesquiterpenos/farmacología , Sesquiterpenos/química , China , Monoterpenos/aislamiento & purificación , Monoterpenos/farmacologíaRESUMEN
The investigation of the leaves of Pittosporum elevaticostatum Chang et Yan led to the isolation of fifteen pentacyclic triterpenoids (1-15), including five previously undescribed ones (1-5), and nine others (16-24). The structures of compounds 1-5 were elucidated based on comprehensive spectroscopic techniques, including one dimension (1D) and 2D nuclear magnetic resonance (NMR), high resolution electrospray ionization mass spectroscopy (HR-ESI-MS), and other methods. Compounds 2 and 13 demonstrated significant inhibitory activity against Listeria monocytogenes (L. monocytogenes) with minimum inhibitory concentration (MIC) values of 32 µM. Scanning electron microscopy (SEM) observations revealed insights into the antibacterial mechanism, indicating that compounds 2 and 13 either prevent biofilm formation of dispersed the preformed cell membranes. Additionally, compounds 1, 5, 7, and 12 exhibited anti-inflammatory activity on lipopolysaccharide (LPS)-stimulated BV-2 microglial cells with IC50 values ranging from 11.27 to 17.80 µM.
Asunto(s)
Antibacterianos , Antiinflamatorios , Listeria monocytogenes , Pruebas de Sensibilidad Microbiana , Triterpenos Pentacíclicos , Hojas de la Planta , Hojas de la Planta/química , Antibacterianos/farmacología , Antibacterianos/aislamiento & purificación , Antiinflamatorios/farmacología , Antiinflamatorios/aislamiento & purificación , Estructura Molecular , Ratones , Animales , Listeria monocytogenes/efectos de los fármacos , Triterpenos Pentacíclicos/farmacología , Triterpenos Pentacíclicos/aislamiento & purificación , Fitoquímicos/farmacología , Fitoquímicos/aislamiento & purificación , Microglía/efectos de los fármacos , Línea Celular , ChinaRESUMEN
Herein, six previously undescribed steroids (1-6), were isolated from leaves and twigs of Aphanamixis polystachya (Wall.) R. N. Parker (Meliaceae). Their structures were elucidated by comprehensive spectroscopic analysis, including HRESIMS, 1D and 2D NMR, UV, and IR. Antiviral activity of these compounds were evaluated. Compounds 1-6 showed varying degrees of inhibitory activity against the severe acute respiratory syndrome coronavirus 2 main protease (SARS-CoV-2 Mpro) at 200 µM.