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1.
Biomed Chromatogr ; 32(8): e4236, 2018 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-29516524

RESUMEN

Isoimperatorin (IP) and phellopterin (PP) are two furocoumarins existing in Angelicae Dahuricae Radix. There is an isopentenyloxyl substituted at C-5 in IP, and an isopentenyloxyl and a methoxyl substituted at C-8 and C-5, respectively, in PP. To elucidate the in vivo metabolic characteristics of PP and IP, HPLC coupled with diode array detector and electrospray ionization ion trap time-of-flight mass spectrometry technique was used. In total, 111 metabolites, including 53 new ones, were identified from the urine and plasma samples of rats after oral administration of IP and PP, respectively. The metabolites were formed through eight reactions on IP and PP: oxidation, hydroxylation-hydrogenation, carboxylation on the isopentenyloxyl, O-dealkylation, hydroxylation on the furocoumarin nucleus, ring-opening reaction on the furan ring and reduction or ring-opening reaction on the lactone ring. Among these, hydroxylation on the furocoumarin nucleus was found for the first time for in vivo metabolites of PP and IP, and the ring-opening reaction on the furan ring or lactone ring was found for the first time for in vivo metabolites of isopentenyloxyl furocoumarins. The research gave us a new insight into the in vivo metabolic profiles of IP and PP, which could help us better understand their important roles as two active constituents of Angelicae Dahuricae Radix.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Cumarinas , Furocumarinas , Espectrometría de Masa por Ionización de Electrospray/métodos , Animales , Cumarinas/sangre , Cumarinas/química , Cumarinas/metabolismo , Cumarinas/orina , Medicamentos Herbarios Chinos/metabolismo , Furocumarinas/sangre , Furocumarinas/química , Furocumarinas/metabolismo , Furocumarinas/orina , Masculino , Redes y Vías Metabólicas , Modelos Moleculares , Ratas , Ratas Sprague-Dawley
2.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1044-1045: 30-38, 2017 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-28064067

RESUMEN

An accurate, precise, selective, and sensitive liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of imperatorin (IMP) and its metabolite, xanthotoxol (XAN), in rat plasma and urine samples. The analytes, along with psoralen as an internal standard, were determined by multiple reaction monitoring (MRM) operated in the positive electrospray ionization (ESI) mode. Chromatographic separation was performed on an Acquity UPLC BEH C18 column (50mm×2.1mm, 1.7µm) with a mobile phase consisting of 0.1% formic acid solution and 0.1% formic acid in methanol at a flow rate of 0.3mL/min. The run time was 6min per sample and the injection volume was 5µL. The method had a lower limit of quantification (LLOQ) of 0.25ng/mL for IMP in plasma and urine, and 1ng/mL for XAN in urine. The linear calibration curves were fitted over the range of 0.25-1000ng/mL for IMP in plasma, 0.25-1000ng/mL for IMP in urine, and 1-1000ng/mL for XAN in urine, with correlation coefficients greater than 0.995. The inter- and intra-day accuracies (relative error, RE%) were between -8.5% and 3.5%, and the precisions (relative standard deviation, RSD%) were less than 10.0% for all quality control samples (QCs). The analytes were extracted from rat plasma and urine samples using a liquid-liquid extraction method with the extraction recovery in the range of 60.3-79.1%. A good stability of the analytes was observed in all the analysis procedures. The method was successfully validated and applied to determine the pharmacokinetics of IMP in rat plasma and, for the first time, the metabolite kinetics of IMP to XAN in rat urine after IMP administration.


Asunto(s)
Cromatografía Liquida/métodos , Furocumarinas/sangre , Furocumarinas/orina , Espectrometría de Masas en Tándem/métodos , Animales , Furocumarinas/metabolismo , Furocumarinas/farmacocinética , Límite de Detección , Modelos Lineales , Masculino , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados
3.
Int J Food Sci Nutr ; 67(8): 881-7, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27396405

RESUMEN

To develop a comprehensive analytical method for photoactive furanocoumarins, grapefruit (whole, flesh, peel and juice) was extracted using QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. Seven furanocoumarins: bergaptol, psoralen, 8-methoxypsoralen, bergapten, 6',7'-dihydroxybergamottin (6',7'-DHB), epoxybergamottin and bergamottin were determined in grapefruit using UPLC-MS/MS. The concentrations of furanocoumarins in the plasma and urine of six healthy young adults before and after ingestion of grapefruit or grapefruit juice were also determined. Recovery rates of furanocoumarins by QuEChERS method from matrix spike sample and laboratory calibrate sample were 125.7 ± 25.4% and 105.7 ± 6.3%, respectively. Bergamottin and 6',7'-DHB were predominant compounds in grapefruit flesh, juice and plasma, while bergaptol and 6',7'-DHB were major compounds detected in the urine. The results demonstrated that bergamottin and 6',7'-DHB were metabolized to bergaptol. Overall, the analytical methods developed in the present study can be applied to the analysis of various furanocoumarins in plant sources and biological samples.


Asunto(s)
Citrus paradisi/química , Furocumarinas/análisis , Adulto , Disponibilidad Biológica , Cromatografía Líquida de Alta Presión/métodos , Femenino , Análisis de los Alimentos/métodos , Jugos de Frutas y Vegetales/análisis , Furocumarinas/sangre , Furocumarinas/orina , Humanos , Masculino , Espectrometría de Masas en Tándem/métodos , Adulto Joven
4.
J Agric Food Chem ; 62(9): 2134-40, 2014 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-24568314

RESUMEN

Grapefruit is a rich source of flavonoids but also contains furanocoumarins, which are known to strongly interact with a variety of medications. Thus, characterization of grapefruit furanocoumarin metabolites may help in a better understanding of grapefruit-drug interactions. In the present work, identification of the main metabolites of grapefruit juice furanocoumarins in urine was performed by ultraperformance liquid chromatography (UPLC) coupled to linear ion trap-Orbitrap mass spectrometry (LTQ-Orbitrap). Glucuronides of 6',7'-dihydroxybergamottin and a hydroxybergamottin-like metabolite were identified for the first time as grapefruit juice metabolites. Afterward, a fast and sensitive method based on solid-phase extraction (SPE) and UPLC coupled to triple quadrupole-tandem mass spectrometry (QqQ-MS/MS) was developed for determination of the identified metabolites in urine. The proposed method was applied to urine samples of five volunteers after intakes of moderate doses of grapefruit, lemon, and orange juices. Furanocoumarin metabolites were only detected in urines after consumption of grapefruit juice.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Citrus paradisi/química , Citrus paradisi/metabolismo , Furocumarinas/química , Extracción en Fase Sólida/métodos , Espectrometría de Masas en Tándem/métodos , Adulto , Furocumarinas/aislamiento & purificación , Furocumarinas/orina , Humanos , Masculino , Adulto Joven
5.
Anal Bioanal Chem ; 405(21): 6721-38, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23881365

RESUMEN

The detection of drug metabolites, especially for minor metabolites, continues to be a challenge because of the complexity of biological samples. Imperatorin (IMP) is an active natural furocoumarin component originating from many traditional Chinese herbal medicines and is expected to be pursued as a new vasorelaxant agent. In the present study, a generic and efficient approach was developed for the in vivo screening and identification of IMP metabolites using liquid chromatography-Triple TOF mass spectrometry. In this approach, a novel on-line data acquisition method mutiple mass defect filter (MMDF) combined with dynamic background subtraction was developed to trace all probable urinary metabolites of IMP. Comparing with the traditionally intensity-dependent data acquisition method, MMDF method could give the information of low-level metabolites masked by background noise and endogenous components. Thus, the minor metabolites in complex biological matrices could be detected. Then, the sensitive and specific multiple data-mining techniques extracted ion chromatography, mass defect filter, product ion filter, and neutral loss filter were used for the discovery of IMP metabolites. Based on the proposed strategy, 44 phase I and 7 phase II metabolites were identified in rat urine after oral administration of IMP. The results indicated that oxidization was the main metabolic pathway and that different oxidized substituent positions had a significant influence on the fragmentation of the metabolites. Two types of characteristic ions at m/z 203 and 219 can be observed in the MS/MS spectra. This is the first study of IMP metabolism in vivo. The interpretation of the MS/MS spectra of these metabolites and the proposed metabolite pathway provide essential data for further pharmacological studies of other linear-type furocoumarins.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Minería de Datos/métodos , Furocumarinas/administración & dosificación , Furocumarinas/orina , Espectrometría de Masa por Ionización de Electrospray/métodos , Espectrometría de Masas en Tándem/métodos , Administración Oral , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
6.
Food Chem Toxicol ; 49(12): 3224-31, 2011 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-21945416

RESUMEN

Furocoumarins are phototoxic and photogenotoxic natural plant constituents occurring in cosmetics, food and drugs. Grapefruit juice is considered as a major dietary source of furocoumarins although few is known about the variability of furocoumarins in grapefruit juice. We analyzed the major furocoumarins in eight commercial grapefruit juices and in freshly prepared juices made from pink grapefruit obtained from German retailers. Bergaptol was the major furocoumarin in commercial juices, followed by bergamottin and 6',7'-dihydroxy-bergamottin (DHB), whereas an inverse picture (DHB>bergamottin>bergaptol) was obtained in freshly prepared juices. Results from different batches of a single brand of commercial juice, purchased over a period of 7 months, revealed a variability of about 50% for the individual furocoumarins and the sum. In a study with healthy volunteers, consumption of 900 ml commercial grapefruit juice (containing 12.5mg bergaptol, 6.9 mg bergamottin, and 0.6 mg DHB) resulted in an average urinary excretion of 0.36 mg free plus 13.23 mg conjugated bergaptol within 6h. Other furocoumarins were not found in urine. Thus, other grapefruit furocoumarins were obviously converted in the human body, at least in part, into bergaptol excreted in urine, since the excreted amount of bergaptol exceeded the consumed one.


Asunto(s)
Bebidas , Citrus paradisi/química , Furocumarinas/orina , Adulto , Femenino , Humanos , Límite de Detección , Masculino , Adulto Joven
7.
J Chromatogr Sci ; 44(8): 473-8, 2006 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-16959122

RESUMEN

A high-performance liquid chromatography method has been developed for the determination of coumarins and furocoumarins (psoralens). Nine coumarins and furocoumarins are separated simultaneously on a Hypersil C(8) (25 cm x 4.6-mm i.d.) column with a gradient of methanol and acetonitrile aqueous solution as mobile phase at 1.0 mL/min with two-channel UV-vis absorbance detection. The limits of detection are 0.366, 0.219, 0.317, 0.440, 0.536, 0.300, 0.531, 0.531, 0.237, and 0.280 ng/mL for coumarin, 7-hydroxycoumarin, 7-methoxycoumarin, citropten (5,7-dimethoxycoumarin), 7-ethoxy-4-methylcoumarin, psoralen, xanthotoxin (8-methoxypsoralen), bergapten (5-methoxypsoralen), isopimpinellin (5,8-dimethoxypsoralen), and imperatorin (9-isopenteneoxypsoralen), respectively. Human urine is analyzed 1-6 days after ingestion of the oral Chinese medicines. This lead to the conclusion that the concentration of coumarins and furocoumarins is higher than that of the control urine. The coumarins and furocoumarins are detected at 312 and 249 nm, respectively.


Asunto(s)
Apiaceae/química , Cromatografía Líquida de Alta Presión/métodos , Furocumarinas/orina , Administración Oral , Humanos , Extractos Vegetales/administración & dosificación , Sensibilidad y Especificidad , Espectrofotometría Ultravioleta
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