RESUMEN
Chronic wounds, especially in diabetic patients, represent a challenging health issue. Since standard treatment protocols often do not provide satisfactory results, additional treatment methods-like phototherapy using low-level light therapy-are being investigated. The aim of our study was to evaluate the effect of phototherapy with light-emitting diodes on chronic wound treatment in diabetic and non-diabetic patients. Since a sufficient blood supply is mandatory for wound healing, the evaluation of microcirculation in the healthy skin at a wound's edge was the main outcome measure. Forty non-diabetic patients and 39 diabetics with lower limb chronic wounds who were referred to the University Medical Center Ljubljana between October 2012 and June 2014 were randomized to the treated and control groups. The treated group received phototherapy with LED 2.4 J/cm2 (wavelengths 625, 660, 850 nm) three times a week for 8 weeks, and the control group received phototherapy with broadband 580-900 nm and power density 0.72 J/cm2. Microcirculation was measured using laser Doppler. A significant increase in blood flow was noted in the treated group of diabetic and non-diabetic patients (p = 0.040 and p = 0.033), while there was no difference in the control groups. Additional Falanga wound bed score evaluation showed a significant improvement in both treated groups as compared to the control group. According to our results, phototherapy with LED was shown to be an effective additional treatment method for chronic wounds in diabetic and non-diabetic patients.
Asunto(s)
Diabetes Mellitus/fisiopatología , Diabetes Mellitus/radioterapia , Microcirculación/efectos de la radiación , Fototerapia , Cicatrización de Heridas/efectos de la radiación , Anciano , Enfermedad Crónica , Método Doble Ciego , Femenino , Hemorreología/efectos de la radiación , Humanos , Masculino , Persona de Mediana Edad , Placebos , Estudios ProspectivosRESUMEN
Physico-chemical adsorption-rheological properties of venous blood in patients, suffering varicose disease of the lower extremities, and their impact on efficacy of various methods of surgical treatment were studied. Conduction of endovasal laser coagulation in combination with crossectomy have promoted enhancement of operative treatment efficacy in patients in initial terms of observation (in 1 week), in 1 month a complete occlusion of the vein was noted more rarely. Efficacy of a small--power laser ablation with irradiation power of 10 W and less in 4 weeks postoperatively is higher, than of surgical treatment with a laser irradiation power 15 W. In a varicose disease of the lower extremities there were observed the raising of the blood volume toughness, superficial relaxation and superficial stress on background of reduction of the toughness--elasticity module, superficial toughness and superficial elasticity. Crossectomy conduction did not influence the integral dynamics of adsorption--rheological properties of venous blood, but in 1 month after endovasal laser coagulation a normalization of physicchemical parameters of blood was noted. Application of laser irradiation of the 10 W power and less promotes inhibition of the relaxation properties of venous blood; a prognostic meaning owes initial value of the blood volume toughness.
Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Extremidad Inferior/cirugía , Vena Safena/cirugía , Várices/radioterapia , Várices/cirugía , Adulto , Angioplastia por Láser/métodos , Femenino , Hemorreología/efectos de la radiación , Humanos , Coagulación con Láser/métodos , Extremidad Inferior/irrigación sanguínea , Extremidad Inferior/patología , Extremidad Inferior/efectos de la radiación , Masculino , Persona de Mediana Edad , Vena Safena/patología , Resultado del Tratamiento , Várices/patologíaRESUMEN
We have investigated impact of electromagnetic SWF-oscillations at nitric oxide molecular spectrum of radiation and absorption (MSRA) on rheological blood properties of albino rats under the immobilizing stress. The SWF-irradiation was demonstrated to foster restoration of disordered rheological blood properties, its efficiency depending on the period of irradiation.
Asunto(s)
Viscosidad Sanguínea/efectos de la radiación , Campos Electromagnéticos , Óxido Nítrico/química , Estrés Psicológico/sangre , Animales , Viscosidad Sanguínea/fisiología , Hemorreología/efectos de la radiación , Masculino , Ratas , Ratas EndogámicasRESUMEN
OBJECTIVE: The purpose of this study was to investigate the in vitro effects of He-Ne laser irradiation on some rheological factors of human blood, such as blood viscosity, erythrocyte deformability, and sedimentation rate. BACKGROUND DATA: The intravascular irradiation of low power laser has been applied in pre-clinical and clinical to treat various pathological processes. However, the mechanism is not fully understood so far. Especially the interaction and related mechanism between the laser and blood are unclear. In this work, by measuring the change of the main rheological factors after laser irradiation, the interaction and mechanism were explored. METHODS: A30-mW He-Ne laser was used for irradiation with a 4-5-mm-diameter beam spot on blood samples, with a fluence rate of about 150 mW/cm.(2) The irradiation time was 60 min, so the total dose of irradiation was 540 J/cm.(2) The pathological samples of blood were obtained from patients (volunteers), and each sample was divided into two tubes for irradiation and control. The blood viscosity, erythrocyte deformability, and sedimentation rate were measured after laser irradiation and compared with un-irradiated control. The blood samples with poor erythrocyte deformability were prepared by adding Ca(2+) to the normal erythrocytes of a healthy person for investigating the laser effect on erythrocyte deformability further. RESULTS: Laser irradiation reduced the erythrocyte sedimentation rate of blood samples, which had a hyper-sedimentation rate originally. The blood viscosity of samples in hyper-values was lowered by laser irradiation in all shear rates measured (10-110 S(-1)), with a relative variation of approximately 10%. The deformability of erythrocytes from pathological samples and Ca(2+)-treated samples was improved after laser irradiation. CONCLUSIONS: The positive effects of laser irradiation on improving the rheological properties of blood were demonstrated in vitro.
Asunto(s)
Viscosidad Sanguínea/efectos de la radiación , Deformación Eritrocítica/efectos de la radiación , Hemorreología/efectos de la radiación , Rayos Láser , Sedimentación Sanguínea , Helio , Hematócrito , Humanos , NeónRESUMEN
A correlation between variations in the medical parameters and the heliogeophysical indices describing physical processes during solar storms was established. It was proposed that one of the reasons for the biotropic effects observed is rheological shifts of human blood during enhancement of solar activity. The influence of heliogeophysical disturbances on the rheological properties of blood was investigated.
Asunto(s)
Enfermedades Cardiovasculares , Actividad Solar , Accidente Cerebrovascular , Enfermedades Cardiovasculares/sangre , Servicios Médicos de Urgencia/estadística & datos numéricos , Hemorreología/efectos de la radiación , Hospitalización/estadística & datos numéricos , Humanos , Moscú , Accidente Cerebrovascular/sangreRESUMEN
BACKGROUND: Blood is often irradiated before transfusion for severely immunocompromised patients to prevent a potentially fatal complication of transfusion-associated GVHD. STUDY DESIGN AND METHODS: This study evaluates the effects of X-ray radiation on platelet and lymphocyte rheology because the ability of these blood cells to deform is vital to their flow throughout the microvascular system. Micropipette aspiration experiments were conducted on platelets and lymphocytes exposed to X-ray radiation doses of 0 (control), 25, and 50 Gy. RESULTS: A significant increase in the Young modulus of elasticity was observed between control platelets and irradiated platelets at 25 Gy (p = 0.02) and 50 Gy (p = 0.03). Percent cell activation increased significantly in 25 Gy-irradiated platelets (p = 0.008). In addition, lymphocytes irradiated at 25 Gy have a higher viscosity than controls (p < 0.02). A significantly larger number of activated cells is found in the 50 Gy-irradiated lymphocyte population (p < 0.04). CONCLUSION: The changes in the deformability and activation of irradiated platelets and lymphocytes may reduce local blood flow and lead to intermittent blockage, which may cause a change of blood flow in microvasculatures.
Asunto(s)
Plaquetas/efectos de la radiación , Hemorreología/efectos de la radiación , Linfocitos/efectos de la radiación , Fenómenos Biomecánicos , Plaquetas/fisiología , Citoplasma/fisiología , Elasticidad , Humanos , Linfocitos/fisiología , Linfocitos/ultraestructura , Activación Plaquetaria/efectos de la radiación , Succión , Viscosidad , Rayos XRESUMEN
A high-dose (7 Gy) whole-body 60Co irradiation for a short period caused disturbances of hematopoietic function. A decrease in the hematocrit of the circulating blood lasted for about 15 days, thus forming an anemic animal model. We studied the influence of high-dose 60Co irradiation on hemorheologic parameters: percentage of reticulocytes, RBC deformability, sedimentation rate and plasma fibrinogen concentration in the rabbit. It was found that the plasma fibrinogen concentration increased to twice more than control level and that percentage of reticulocytes in circulation disappeared immediately after irradiation. The deformation index of RBCs in shear flow decreased from a value of 58% down to a value of 42% in the first two weeks and gradually returned to control levels about 40 days after 60Co irradiation. Our results showed that a short period of high-dose 60Co irradiation caused severe and relatively long-lasting damage of hematopoietic system in animals' body.
Asunto(s)
Radioisótopos de Cobalto/farmacología , Hemorreología/efectos de la radiación , Animales , Sedimentación Sanguínea/efectos de la radiación , Deformación Eritrocítica/efectos de la radiación , Eritrocitos/efectos de la radiación , Femenino , Fibrinógeno/metabolismo , Fibrinógeno/efectos de la radiación , Hematócrito , Masculino , Conejos , Reticulocitos/efectos de la radiación , Irradiación Corporal TotalRESUMEN
The rheological, coagulatory and clinical examination of 70 patients with acute ischemic stroke has demonstrated that EHF therapy (53.53 GHz) improves rheological and coagulatory parameters in such patients, has a positive effect on hemostasis and clinical course of acute ischemic stroke. The technique and doses are presented.
Asunto(s)
Coagulación Sanguínea/efectos de la radiación , Isquemia Encefálica/radioterapia , Microondas/uso terapéutico , Enfermedad Aguda , Isquemia Encefálica/sangre , Hemorreología/efectos de la radiación , HumanosRESUMEN
The authors made an analysis of results of examination of 41 patients with ischemic heart disease treated by the standard medicamentous therapy and when using different methods of photohemotherapy against its background. It was established that medicamentous therapy during 2 weeks failed to result in a substantial improvement of rheological properties of blood, while its combination with photohemotherapy could give a considerable positive effect coinciding with clinical improvement of the patient's state. Shorter terms are required to correct hemorheological indices when autotransfusions of photomodified blood are used.
Asunto(s)
Transfusión de Sangre Autóloga/métodos , Sangre/efectos de la radiación , Hemorreología/efectos de la radiación , Isquemia Miocárdica/terapia , Terapia Ultravioleta/métodos , Anciano , Transfusión de Sangre Autóloga/instrumentación , Viscosidad Sanguínea/efectos de los fármacos , Viscosidad Sanguínea/efectos de la radiación , Fármacos Cardiovasculares/uso terapéutico , Cateterismo Periférico , Terapia Combinada , Quimioterapia Combinada , Femenino , Hemorreología/efectos de los fármacos , Humanos , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/sangre , Factores de Tiempo , Terapia Ultravioleta/instrumentaciónRESUMEN
OBJECTIVE: The purpose of this study was to investigate the in vitro effects of low-level laser radiation (LLLR) on some rheological factors of the human blood, such as complete blood count (CBC) parameters and blood sedimentation rate (BSR). We were mainly concerned with the alterations caused by LLLR action on blood cells (erythrocytes and leukocytes) of fresh blood obtained from apparently healthy adult patients. We used low doses ranging between 0.80 J x cm(-3) and 4.40 J x cm(-3), at the very low-power densities of the laser radiation, so as not to damage the cell structure and not to alter in an undesired manner their functions. METHODS: Blood samples were taken from 22 volunteers. Where health problems existed, they were indicated for each case. The parameters mentioned above were measured before (control samples) and after irradiation. A He-Ne laser, operating in continuous wave, as a radiation source (632.8 nm, 1 mW, intensity of 8 x 10(-2) W x sr(-1), mean power density incident on blood samples around 30 mW x cm(-2), beam spot diameter 2 mm) was used. The measurements were performed immediately after irradiation. Only the erythrocyte complex was irradiated. EDTA anticoagulant was used. RESULTS: The measurements using a computerized hemoanalyzer type SERONO showed significant differences between control and irradiated blood samples concerning the following parameters: RBC (in 22% of cases), HGB (47.3%), HCT (84.2%), RDW (11%), PLT (5.26%), MPV (33.3%), WBC (5.26%), MONO (26.3%), and GRAN (63.15%). In the case of BSR (44%), the significant differences were noticed especially in the cases of patients suffering from some acute or chronic diseases. Nonsignificant differences were noticed in the cases of MCV, MCH, MCHC, RDW, and LYMPH. CONCLUSIONS: This study has shown that LLLR, even though used at low doses and low power densities, produced some changes of the rheological factors of the blood, as follows: a revitalizing and regenerating effect on mitosis stimulation and a nondamaging and biostimulating effect on the cell membrane (by keeping unmodified MCV, MCH, and MCHC). In 3 cases out of 22, hemolysis (complete or partially) occurred, but we are not yet sure whether this was caused by laser exposure or by certain environmental physical factors.
Asunto(s)
Hemorreología/efectos de la radiación , Rayos Láser , Adulto , Anciano , Sedimentación Sanguínea , Femenino , Hemólisis/efectos de la radiación , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Results are submitted of investigation designed to study effects of a combined use of plasmapheresis and laser irradiation of blood on hemorheologic and hemodynamic characteristics in 36 patients with stage II hypertensive disease. The course exposure of patients to a combined use of plasmapheresis and laser irradiation of blood led to a drop of arterial pressure in different hemodynamic groups at the expense of different parameters characterising the hemodynamic status. Thus combined use of plasmapheresis and laser irradiation of blood can be considered a promising nonmedicamentous therapeutic alternative in patients with hypertensive disease being associated with a drop of arterial blood pressure, and what is more, improvement in viscous- and elastic properties of blood as well as its hemodynamic indices.
Asunto(s)
Sangre/efectos de la radiación , Hemorreología/efectos de la radiación , Hipertensión/terapia , Terapia por Láser , Plasmaféresis , Adulto , Viscosidad Sanguínea/efectos de la radiación , Terapia Combinada , Hemodinámica/efectos de la radiación , Humanos , Hipertensión/sangre , Hipertensión/fisiopatología , Persona de Mediana Edad , Inducción de RemisiónRESUMEN
158 patients with essential hypertension received beta-adrenoblockers and were exposed to travelling impulse magnetic field, magnetolaser radiation. The study of platelet-vessel hemostasis and blood rheology revealed a relation of good clinical response and increased exercise tolerance with initial platelet dysfunction and rheological disorders which underwent positive changes in the course of treatment.