RESUMEN
The study aimed to evaluate the impact of selected factors of the freeze-drying process on the hydrolytic and synthetic activity of the extracellular lipases of Y. lipolytica KKP 379 and to attempt the use of the crude enzyme preparation as a biocatalyst in the synthesis of geranyl 4-hydroxyphenylpropanoate. Antioxidant and antibacterial properties of the geranyl ester derivative were also investigated in order to evaluate their usefulness as a novel food additive. The studies confirmed that freeze-drying was an effective method of dehydrating yeast supernatant and allowed for obtaining lyophilizates with low water activity from 0.055 to 0.160. The type and concentration of the additive (2-6% whey protein hydrolyzate, 0.5% and 1% ammonium sulphate) had a significant effect on the hydrolytic activity of enzyme preparations, while the selected variants of drying temperature during the freeze-drying process were not significant (10 °C and 50 °C). Low yield of geranyl 4-hydroxyphenylopropionate was shown when the lyophilized supernatant was used (5.3%), but the yield of ester synthesis increased when the freeze-dried Y. lipolytica yeast biomass was applied (47.9%). The study confirmed the antioxidant properties of the synthesized ester by the DPPH⢠and CUPRAC methods, as well as higher antibacterial activity against tested bacteria than its precursor with 0.125 mM MIC (minimal inhibitory concentration) against L. monocytogenes.
Asunto(s)
Monoterpenos Acíclicos/metabolismo , Líquido Extracelular/enzimología , Lactatos/metabolismo , Lipasa/metabolismo , Yarrowia/enzimología , Monoterpenos Acíclicos/síntesis química , Antibacterianos/síntesis química , Antibacterianos/metabolismo , Antioxidantes/síntesis química , Antioxidantes/metabolismo , Catálisis , Ésteres , Liofilización/métodos , Lactatos/síntesis química , Lipasa/química , Pruebas de Sensibilidad Microbiana/métodos , Yarrowia/químicaRESUMEN
Bone is one of the major tissues that undergoes continuous remodeling throughout life, thus ensuring both organic body growth during development and protection of internal organs as well as repair of trauma during adulthood. Many endogenous substances contribute to bone homeostasis, including purines. Their role has increasingly emerged in recent decades as compounds which, by interacting with specific receptors, can help determine adequate responses of bone cells to physiological or pathological stimuli. Equally, it is recognized that the activity of purines is closely dependent on their interconversion or metabolic degradation ensured by a series of enzymes present at extracellular level as predominantly bound to the cell membrane or, also, as soluble isoforms. While the effects of purines mediated by their receptor interactions have sufficiently, even though not entirely, been characterized in many tissues including bone, those promoted by the extracellular enzymes providing for purine metabolism have not been. In this review, we will try to circumstantiate the presence and the role of these enzymes in bone to define their close relationship with purine activities in maintaining bone homeostasis in normal or pathological conditions.
Asunto(s)
Huesos/fisiología , Líquido Extracelular/enzimología , Líquido Extracelular/metabolismo , Homeostasis , Purinas/metabolismo , Receptores Purinérgicos/metabolismo , Animales , Humanos , Transducción de SeñalRESUMEN
The crucial role of extracellular proteases in cancer progression is well-known, especially in relation to the promotion of cell invasion through extracellular matrix remodeling. This also occurs by the ability of extracellular proteases to induce the shedding of transmembrane proteins at the plasma membrane surface or within extracellular vesicles. This process results in the regulation of key signaling pathways by the modulation of kinases, e.g., the epidermal growth factor receptor (EGFR). Considering their regulatory roles in cancer, therapeutics targeting various extracellular proteases have been discovered. These include the metal-binding agents di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) and di-2-pyridylketone-4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), which increase c-MET degradation by multiple mechanisms. Both the direct and indirect inhibition of protease expression and activity can be achieved through metal ion depletion. Considering direct mechanisms, chelators can bind zinc(II) that plays a catalytic role in enzyme activity. In terms of indirect mechanisms, Dp44mT and DpC potently suppress the expression of the kallikrein-related peptidase-a prostate-specific antigen-in prostate cancer cells. The mechanism of this activity involves promotion of the degradation of the androgen receptor. Additional suppressive mechanisms of Dp44mT and DpC on matrix metalloproteases (MMPs) relate to their ability to up-regulate the metastasis suppressors N-myc downstream regulated gene-1 (NDRG1) and NDRG2, which down-regulate MMPs that are crucial for cancer cell invasion.
Asunto(s)
Antineoplásicos/uso terapéutico , Quelantes/uso terapéutico , Hierro , Proteínas de Neoplasias/fisiología , Péptido Hidrolasas/fisiología , Inhibidores de Proteasas/uso terapéutico , Zinc , Antineoplásicos/farmacología , Línea Celular Tumoral , Transformación Celular Neoplásica , Quelantes/farmacología , Progresión de la Enfermedad , Diseño de Fármacos , Ensayos de Selección de Medicamentos Antitumorales , Líquido Extracelular/enzimología , Vesículas Extracelulares/enzimología , Humanos , Ácidos Hidroxámicos/farmacología , Ácidos Hidroxámicos/uso terapéutico , Quelantes del Hierro/farmacología , Quelantes del Hierro/uso terapéutico , Calicreínas/antagonistas & inhibidores , Calicreínas/fisiología , Metaloproteinasas de la Matriz/fisiología , Terapia Molecular Dirigida , Proteínas de Neoplasias/antagonistas & inhibidores , Oxaprozina/farmacología , Oxaprozina/uso terapéutico , Fenilalanina/análogos & derivados , Fenilalanina/farmacología , Fenilalanina/uso terapéutico , Inhibidores de Proteasas/farmacología , Proteínas Quinasas/fisiología , Piridinas/farmacología , Piridinas/uso terapéutico , Tiofenos/farmacología , Tiofenos/uso terapéutico , Tiosemicarbazonas/farmacología , Tiosemicarbazonas/uso terapéuticoRESUMEN
Amyotrophic lateral sclerosis (ALS) is characterized by adult-onset progressive degeneration of upper and lower motor neurons. Increasing numbers of genes are found to be associated with ALS; among those, the first identified gene, SOD1 coding a Cu/Zn-superoxide dismutase protein (SOD1), has been regarded as the gold standard in the research on a pathomechanism of ALS. Abnormal accumulation of misfolded SOD1 in affected spinal motor neurons has been established as a pathological hallmark of ALS caused by mutations in SOD1 (SOD1-ALS). Nonetheless, involvement of wild-type SOD1 remains quite controversial in the pathology of ALS with no SOD1 mutations (non-SOD1 ALS), which occupies more than 90% of total ALS cases. In vitro studies have revealed post-translationally controlled misfolding and aggregation of wild-type as well as of mutant SOD1 proteins; therefore, SOD1 proteins could be a therapeutic target not only in SOD1-ALS but also in more prevailing cases, non-SOD1 ALS. In order to search for evidence on misfolding and aggregation of wild-type SOD1 in vivo, we reviewed pathological studies using mouse models and patients and then summarized arguments for and against possible involvement of wild-type SOD1 in non-SOD1 ALS as well as in SOD1-ALS.
Asunto(s)
Esclerosis Amiotrófica Lateral/enzimología , Esclerosis Amiotrófica Lateral/patología , Pliegue de Proteína , Superóxido Dismutasa-1/metabolismo , Esclerosis Amiotrófica Lateral/genética , Animales , Líquido Extracelular/enzimología , Humanos , Neuronas Motoras/enzimología , Neuronas Motoras/patología , Superóxido Dismutasa-1/genéticaRESUMEN
A human single nucleotide polymorphism (SNP) in the matrix-binding domain of extracellular superoxide dismutase (EC-SOD), with arginine to glycine substitution at position 213 (R213G), redistributes EC-SOD from the matrix into extracellular fluids. We reported that, following bleomycin (bleo), knockin mice harboring the human R213G SNP (R213G mice) exhibit enhanced resolution of inflammation and protection against fibrosis, compared with wild-type (WT) littermates. In this study, we tested the hypothesis that the EC-SOD R213G SNP promotes resolution via accelerated apoptosis of recruited alveolar macrophage (AM). RNA sequencing and Ingenuity Pathway Analysis 7 d postbleo in recruited AM implicated increased apoptosis and blunted inflammatory responses in the R213G strain exhibiting accelerated resolution. We validated that the percentage of apoptosis was significantly elevated in R213G recruited AM vs. WT at 3 and 7 d postbleo in vivo. Recruited AM numbers were also significantly decreased in R213G mice vs. WT at 3 and 7 d postbleo. ChaC glutathione-specific γ-glutamylcyclotransferase 1 (Chac1), a proapoptotic γ-glutamyl cyclotransferase that depletes glutathione, was increased in the R213G recruited AM. Overexpression of Chac1 in vitro induced apoptosis of macrophages and was blocked by administration of cell-permeable glutathione. In summary, we provide new evidence that redistributed EC-SOD accelerates the resolution of inflammation through redox-regulated mechanisms that increase recruited AM apoptosis.-Allawzi, A., McDermott, I., Delaney, C., Nguyen, K., Banimostafa, L., Trumpie, A., Hernandez-Lagunas, L., Riemondy, K., Gillen, A., Hesselberth, J., El Kasmi, K., Sucharov, C. C., Janssen, W. J., Stenmark, K., Bowler, R., Nozik-Grayck, E. Redistribution of EC-SOD resolves bleomycin-induced inflammation via increased apoptosis of recruited alveolar macrophages.
Asunto(s)
Apoptosis , Bleomicina/toxicidad , Líquido Extracelular/enzimología , Matriz Extracelular/enzimología , Inflamación/prevención & control , Macrófagos Alveolares/patología , Superóxido Dismutasa/metabolismo , Animales , Antibióticos Antineoplásicos/toxicidad , Células Cultivadas , Femenino , Fibrosis/inducido químicamente , Fibrosis/metabolismo , Fibrosis/prevención & control , Humanos , Inflamación/inducido químicamente , Inflamación/metabolismo , Pulmón/efectos de los fármacos , Pulmón/inmunología , Pulmón/metabolismo , Pulmón/patología , Macrófagos Alveolares/efectos de los fármacos , Macrófagos Alveolares/inmunología , Macrófagos Alveolares/metabolismo , Ratones , Ratones Endogámicos C57BL , Polimorfismo de Nucleótido Simple , Superóxido Dismutasa/genéticaRESUMEN
Damage-associated molecular patterns (DAMPs) are molecules that can be actively or passively released by injured tissues and that activate the immune system. Here we show that nicotinate phosphoribosyltransferase (NAPRT), detected by antibody-mediated assays and mass spectrometry, is an extracellular ligand for Toll-like receptor 4 (TLR4) and a critical mediator of inflammation, acting as a DAMP. Exposure of human and mouse macrophages to NAPRT activates the inflammasome and NF-κB for secretion of inflammatory cytokines. Furthermore, NAPRT enhances monocyte differentiation into macrophages by inducing macrophage colony-stimulating factor. These NAPRT-induced effects are independent of NAD-biosynthetic activity, but rely on NAPRT binding to TLR4. In line with our finding that NAPRT mediates endotoxin tolerance in vitro and in vivo, sera from patients with sepsis contain the highest levels of NAPRT, compared to patients with other chronic inflammatory conditions. Together, these data identify NAPRT as a endogenous ligand for TLR4 and a mediator of inflammation.
Asunto(s)
Espacio Extracelular/metabolismo , Inflamación/enzimología , Pentosiltransferasa/metabolismo , Receptor Toll-Like 4/metabolismo , Diferenciación Celular , Líquido Extracelular/enzimología , Humanos , Inflamación/genética , Inflamación/patología , Factor Estimulante de Colonias de Macrófagos/metabolismo , Macrófagos/metabolismo , Monocitos/citología , Células Mieloides/metabolismo , Nicotinamida Fosforribosiltransferasa/química , Nicotinamida Fosforribosiltransferasa/metabolismo , Pentosiltransferasa/sangre , Pentosiltransferasa/química , Unión Proteica , Factores de Riesgo , Sepsis/sangre , Sepsis/enzimologíaRESUMEN
Interaction of truffle mycelium with the host plant involves the excretion of extracellular enzymes. The ability of Tuber maculatum mycelium to produce an extracellular cellulase during submerged fermentation was demonstrated for the first time. T. maculatum mycelia were isolated and tested for extracellular cellulase production at variable pH on solid agar medium, and the highest activity was observed at pH 7.0. Furthermore, T. maculatum was subjected to submerged fermentation in basal salt medium for cellulase production. Under optimized conditions using sodium carboxymethyl cellulose (0.5 % w/v) as carbon source and an initial pH of 7.0, the enzyme production yielded 1.70 U/mL of cellulase in the cell-free supernatant after 7 days of incubation time. The optimum of the obtained cellulase's activity was at pH 5.0 and a temperature of 50 °C. The enzyme showed good thermostability at 50 °C by retaining 99 % of its maximal activity over an incubation time of 100 min. The cellulase activity was inhibited by Fe2+ and slightly activated by Mn2+ and Cu2+ at 1 mM concentration. The results indicated that truffle mycelium is utilizing cellulosic energy source in the root system, and the optimal conditions are those existing in the acidic Finnish soil.
Asunto(s)
Ascomicetos/enzimología , Reactores Biológicos/microbiología , Celulasa/química , Celulasa/metabolismo , Celulosa/química , Líquido Extracelular/enzimología , Ascomicetos/crecimiento & desarrollo , Celulasa/aislamiento & purificación , Activación Enzimática , Estabilidad de Enzimas , Especificidad por SustratoRESUMEN
Gut-brain hormones such as ghrelin have recently been suggested to have a role in reward regulation. Ghrelin was traditionally known to regulate food intake and body weight homoeostasis. In addition, recent work has pin-pointed that this peptide has a novel role in drug-induced reward, including morphine-induced increase in the extracellular levels of accumbal dopamine in rats. Herein the effect of the ghrelin receptor (GHS-R1A) antagonist, JMV2959, on morphine-induced activation of the mesolimbic dopamine system was investigated in mice. In addition, the effects of JMV2959 administration on opioid peptide levels in reward related areas were investigated. In the present series of experiment we showed that peripheral JMV2959 administration, at a dose with no effect per se, attenuates the ability of morphine to cause locomotor stimulation, increase the extracellular levels of accumbal dopamine and to condition a place preference in mice. JMV2959 administration significantly increased tissue levels of Met-enkephalin-Arg(6)Phe(7) in the ventral tegmental area, dynorphin B in hippocampus and Leu-enkephalin-Arg(6) in striatum. We therefore hypothesise that JMV2959 prevents morphine-induced reward via stimulation of delta receptor active peptides in striatum and ventral tegmental areas. In addition, hippocampal peptides that activate kappa receptor may be involved in JMV2959׳s ability to regulate memory formation of reward. Given that development of drug addiction depends, at least in part, of the effects of addictive drugs on the mesolimbic dopamine system the present data suggest that GHS-R1A antagonists deserve to be elucidated as novel treatment strategies of opioid addiction.
Asunto(s)
Analgésicos Opioides/farmacología , Encéfalo/metabolismo , Glicina/análogos & derivados , Morfina/farmacología , Péptidos Opioides/farmacología , Receptores de Ghrelina/antagonistas & inhibidores , Recompensa , Triazoles/farmacología , Animales , Encéfalo/efectos de los fármacos , Condicionamiento Operante/efectos de los fármacos , Dopamina/metabolismo , Dinorfinas/metabolismo , Endorfinas/metabolismo , Líquido Extracelular/efectos de los fármacos , Líquido Extracelular/enzimología , Líquido Extracelular/metabolismo , Glicina/farmacología , Masculino , Ratones , Microdiálisis , Actividad Motora/efectos de los fármacosRESUMEN
Myeloperoxidase (MPO) is an important neutrophil lysosomal enzyme, a major autoantigen, and a potential mediator of tissue injury in MPO-ANCA-associated vasculitis (MPO-AAV) and glomerulonephritis. Here we examined MPO deposition in kidney biopsies from 47 patients with MPO-AAV. Leukocyte accumulation and fibrin deposition consistent with cell-mediated immunity was a major feature. Tubulointerstitial macrophage, CD4+ and CD8+ T-cell, and neutrophil numbers correlated with low presenting eGFR. MPO was not detected in kidneys from patients with minimal change or thin basement membrane disease, but was prominent in glomerular, periglomerular, and tubulointerstitial regions in MPO-AAV. Extracellular MPO released from leukocytes was pronounced in all MPO-AAV patients. Similar numbers of neutrophils and macrophages expressed MPO in the kidneys, but colocalization studies identified neutrophils as the major source of extracellular MPO. Extraleukocyte MPO was prominent in neutrophil extracellular traps in the majority of patients; most of which had traps in half or more glomeruli. These traps were associated with more neutrophils and more MPO within glomeruli. Glomerular MPO-containing macrophages generated extracellular trap-like structures. MPO also localized to endothelial cells and podocytes. The presence of the most active glomerular lesions (both segmental necrosis and cellular crescents) correlated with intraglomerular CD4+ cells and MPO+ macrophages. Thus, cellular and extracellular MPO may cause glomerular and interstitial injury.
Asunto(s)
Anticuerpos Anticitoplasma de Neutrófilos , Enfermedades Autoinmunes/enzimología , Trampas Extracelulares/enzimología , Glomerulonefritis/enzimología , Peroxidasa/metabolismo , Anciano , Anticuerpos Anticitoplasma de Neutrófilos/inmunología , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/patología , Recuento de Linfocito CD4 , Linfocitos T CD4-Positivos , Linfocitos T CD8-positivos , Células Dendríticas/enzimología , Células Endoteliales/enzimología , Líquido Extracelular/enzimología , Femenino , Tasa de Filtración Glomerular , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Humanos , Glomérulos Renales/enzimología , Glomérulos Renales/patología , Macrófagos/enzimología , Masculino , Neutrófilos/enzimología , Podocitos/enzimologíaRESUMEN
Cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phylogenetically conserved, ubiquitous enzyme that plays an indispensable role in energy metabolism. Although a wealth of information is available on cellular GAPDH, there is a clear paucity of data on its extracellular counterpart (i.e., the secreted or extracellular GAPDH). Here, we show that the extracellular GAPDH in human serum is a multimeric, high-molecular-weight, yet glycolytically active enzyme. The high-molecular-weight multimers of serum GAPDH were identified by immunodetection on one- and two-dimensional gel electrophoresis using multiple antibodies specific for various epitopes of GAPDH. Partial purification of serum GAPDH by DEAE Affigel affinity/ion exchange chromatography further established the multimeric composition of serum GAPDH. In vitro data demonstrated that human cell lines secrete a multimeric, high-molecular-weight enzyme similar to that of serum GAPDH. Furthermore, LC-MS/MS analysis of extracellular GAPDH from human cell lines confirmed the presence of unique peptides of GAPDH in the high-molecular-weight subunits. Furthermore, data from pulse-chase experiments established the presence of high-molecular-weight subunits in the secreted, extracellular GAPDH. Taken together, our findings demonstrate the presence of a high-molecular-weight, enzymatically active secretory GAPDH in human serum that may have a hitherto unknown function in humans.
Asunto(s)
Líquido Extracelular/enzimología , Gliceraldehído-3-Fosfato Deshidrogenasas/análisis , Suero/enzimología , Secuencia de Aminoácidos , Animales , Línea Celular , Cromatografía por Intercambio Iónico , Cromatografía Liquida , Electroforesis en Gel Bidimensional , Gliceraldehído-3-Fosfato Deshidrogenasas/genética , Humanos , Mamíferos , Datos de Secuencia Molecular , Peso Molecular , Multimerización de Proteína , Espectrometría de Masas en TándemRESUMEN
Unilateral ureteral obstruction (UUO) induced tubulointerstitial fibrosis in kidneys mimics the pathogenesis of chronic kidney diseases and is considered a suitable model for studying the mechanisms leading to fibrosis. To study the role of cyclooxygenase-2 (COX-2) in kidney fibrosis, we investigated whether a selective COX-2 inhibitor, celecoxib, affected renal interstitial fibrosis during UUO in mice. To induce UUO, the left proximal ureter was ligated in male C57BL/6 mice. The mice were fed a diet with or without celecoxib from the day of UUO induction. Following UUO, the renal pelvis was observed to be dilated and the kidney cortex was significantly thinner than that of sham-operated mice. Immunofluorescent staining of type I, III, and IV collagen in UUO kidneys revealed that interstitial collagen deposition was significantly increased in the celecoxib-treated group. Expression of type I, III, and IV collagen in UUO kidneys was also significantly higher in the celecoxib-treated group than in the vehicle-treated group. In the celecoxib-treated group, mRNA levels of TGF-ß/FGF-2 were also significantly higher than those in the vehicle-treated group. The present study demonstrates that COX-2 plays a protective role against fibrosis in UUO kidneys and suggests that supplementation of COX-2 products, such as PG analogues, will be a good option for preventing interstitial fibrosis.
Asunto(s)
Ciclooxigenasa 2/fisiología , Enfermedades Renales/enzimología , Obstrucción Ureteral/enzimología , Animales , Inhibidores de la Ciclooxigenasa 2/uso terapéutico , Líquido Extracelular/efectos de los fármacos , Líquido Extracelular/enzimología , Fibrosis/tratamiento farmacológico , Fibrosis/enzimología , Fibrosis/patología , Enfermedades Renales/tratamiento farmacológico , Enfermedades Renales/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Obstrucción Ureteral/tratamiento farmacológico , Obstrucción Ureteral/patologíaRESUMEN
The bone marrow provides a protected environment for generating a vast array of cell types. Bones are thus a dynamic source of structural components and soluble factors used either locally or at a distance from their site of production. We discuss the role of ectoenzymes in the bone niche where human myeloma grows. Selected ectoenzymes have been tested for their ability to promote production of substrates involved in signaling, synthesis of growth factors and hormones, and modulation of the immune response. Because of the difficulty of simultaneously tracking all these activities, we narrow our focus to events potentially influencing synthesis of adenosine (ADO), an important regulator of multiple biological functions, including local immunological tolerance. Our working hypothesis, to be discussed and partially tested herein, is that CD38, and likely BST1/CD157--both NAD(+) -consuming enzymes, are active in the myeloma niche and lead a discontinuous chain of ectoenzymes whose final products are exploited by the neoplastic plasma cell as part of its local survival strategy. Coadjuvant ectoenzymes include PC-1/CD203a, CD39, and CD73, which control the production of ADO. Results discussed here and from ongoing experiments indicate that the myeloma niche hosts the canonical, as well as alternative, pathways of ADO generation. Other possibilities are presented and discussed.
Asunto(s)
ADP-Ribosil Ciclasa 1/metabolismo , Médula Ósea/enzimología , Mieloma Múltiple/enzimología , Hidrolasas Diéster Fosfóricas/metabolismo , Pirofosfatasas/metabolismo , Adenosina/metabolismo , Animales , Médula Ósea/patología , Supervivencia Celular/fisiología , Líquido Extracelular/enzimología , Humanos , Mieloma Múltiple/patologíaRESUMEN
Lignocellulose facilitates the fungal oxidization of recalcitrant organic pollutants through the extracellular ligninolytic enzymes induced by lignin in wood or other plant tissues. However, available information on this phenomenon is insufficient. Free radical chain reactions during lignin metabolism are important in xenobiotic removal. Thus, the effect of lignin on azo dye decolorization in vivo by Echinodontium taxodii was evaluated. In the presence of lignin, optimum decolorization percentages for Remazol Brilliant Violet 5R, Direct Red 5B, Direct Black 38, and Direct Black 22 were 91.75% (control, 65.96%), 76.89% (control, 43.78%), 43.44% (control, 17.02%), and 44.75% (control, 12.16%), respectively, in the submerged cultures. Laccase was the most important enzyme during biodecolorization. Aside from the stimulating of laccase activity, lignin might be degraded by E. taxodii, and then these degraded low-molecular-weight metabolites could act as redox mediators promoting decolorization of azo dyes. The relationship between laccase and lignin degradation was investigated through decolorization tests in vitro with purified enzyme and dozens of aromatics, which can be derivatives of lignin and can function as laccase mediators or inducers. Dyes were decolorized at triple or even higher rates in certain laccase-aromatic systems at chemical concentrations as low as 10 µM.
Asunto(s)
Compuestos Azo/química , Compuestos Azo/metabolismo , Basidiomycota/efectos de los fármacos , Basidiomycota/metabolismo , Contaminantes Ambientales/química , Contaminantes Ambientales/metabolismo , Lignina/farmacología , Compuestos Azo/aislamiento & purificación , Basidiomycota/citología , Basidiomycota/enzimología , Biodegradación Ambiental/efectos de los fármacos , Color , Técnicas de Cultivo , Contaminantes Ambientales/aislamiento & purificación , Líquido Extracelular/efectos de los fármacos , Líquido Extracelular/enzimología , Hidrólisis/efectos de los fármacos , Lacasa/metabolismo , Lignina/metabolismoRESUMEN
ß-Fructofuranosidases or invertases (EC 3.2.1.26) are enzymes that are widely used in the food industry, where fructose is preferred over sucrose, because it is sweeter and does not crystallize easily. Since Aspergillus niger GH1, an xerophilic fungus from the Mexican semi-desert, has been reported to be an invertase producer, and because of the need for new enzymes with biotechnological applications, in this work, we describe the gene and amino acid sequence of the invertase from A. niger GH1, and the use of a synthetic gene to produce the enzyme in the methylotrophic yeast Pichia pastoris. In addition, the produced invertase was characterized biochemically. The sequence of the invertase gene had a length of 1770 bp without introns, encodes a protein of 589 amino acids, and presented an identity of 93% and 97% with invertases from Aspergillus kawachi IFO 4308 and A. niger B60, respectively. A 4.2 L culture with the constructed recombinant P. pastoris strain showed an extracellular and periplasmic invertase production at 72 h induction of 498 and 3776 invertase units (U), respectively, which corresponds to 1018 U/L of culture medium. The invertase produced had an optimum pH of 5.0, optimum temperature of 60 °C, and specific activity of 3389 U/mg protein, and after storage for 96 h at 4 °C showed 93.7% of its activity. This invertase could be suitable for producing inverted sugar used in the food industry.
Asunto(s)
Aspergillus niger/genética , Proteínas Fúngicas/genética , beta-Fructofuranosidasa/genética , Secuencia de Aminoácidos , Aspergillus niger/enzimología , Secuencia de Bases , Sistema Libre de Células , Clonación Molecular , Líquido Extracelular/enzimología , Fructosa/biosíntesis , Proteínas Fúngicas/aislamiento & purificación , Proteínas Fúngicas/metabolismo , Genes Sintéticos , Glucosa/biosíntesis , Concentración de Iones de Hidrógeno , Microbiología Industrial/métodos , Periplasma/enzimología , Pichia , Estabilidad Proteica , Proteínas Recombinantes de Fusión/metabolismo , Homología de Secuencia , Sacarosa/metabolismo , Temperatura , beta-Fructofuranosidasa/aislamiento & purificación , beta-Fructofuranosidasa/metabolismoRESUMEN
Superoxide dismutase (SOD) activity in flushings from oviducts and uterine horns of 8 anestrous, 5 estrous and 7 diestrous bitches was measured. SOD activity in oviductal fluid in estrous bitches was significantly higher than that in anestrous and diestrous bitches (P<0.01). SOD activity in uterine fluid of diestrous bitches was, however, significantly higher than that in anestrous and estrous bitches (P<0.01). Additionally, sperm collected from normal dogs were incubated in MEM and in MEM containing SOD (SOD-MEM) for 24 hr. The percentages of sperm with viability, motility and hyperactivation in SOD-MEM were higher than those in MEM. SOD produced in oviduct and uterus may be able to maintain or improve sperm quality and fertility in the dog.
Asunto(s)
Líquido Extracelular/enzimología , Oviductos/enzimología , Espermatozoides/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Útero/enzimología , Animales , Supervivencia Celular/efectos de los fármacos , Perros , Ciclo Estral/metabolismo , Femenino , Masculino , Motilidad Espermática/efectos de los fármacos , Superóxido Dismutasa/farmacologíaRESUMEN
BACKGROUND: Atherosclerosis and vascular remodeling after injury are driven by inflammation and mononuclear cell infiltration. Extracellular RNA (eRNA) has recently been implicated to become enriched at sites of tissue damage and to act as a proinflammatory mediator. Here, we addressed the role of eRNA in high-fat diet-induced atherosclerosis and neointima formation after injury in atherosclerosis-prone mice. METHODS AND RESULTS: The presence of eRNA was revealed in atherosclerotic lesions from high-fat diet-fed low-density lipoprotein receptor-deficient (Ldlr(-/-)) mice in a time-progressive fashion. RNase activity in plasma increased within the first 2 weeks (44±9 versus 70±7 mU/mg protein; P=0.0012), followed by a decrease to levels below baseline after 4 weeks of high-fat diet (44±9 versus 12±2 mU/mg protein; P<0.0001). Exposure of bone marrow-derived macrophages to eRNA resulted in a concentration-dependent upregulation of the proinflammatory mediators tumor necrosis factor-α, arginase-2, interleukin-1ß, interleukin-6, and interferon-γ. In a model of accelerated atherosclerosis after arterial injury in apolipoprotein E-deficient (ApoE(-/-)) mice, treatment with RNase1 diminished the increased plasma level of eRNA evidenced after injury. Likewise, RNase1 administration reduced neointima formation in comparison with vehicle-treated ApoE(-/-) controls (25.0±6.2 versus 46.9±6.9×10(3) µm(2), P=0.0339) and was associated with a significant decrease in plaque macrophage content. Functionally, RNase1 treatment impaired monocyte arrest on activated smooth muscle cells under flow conditions in vitro and inhibited leukocyte recruitment to injured carotid arteries in vivo. CONCLUSIONS: Because eRNA is associated with atherosclerotic lesions and contributes to inflammation-dependent plaque progression in atherosclerosis-prone mice, its targeting with RNase1 may serve as a new treatment option against atherosclerosis.
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Líquido Extracelular/fisiología , Placa Aterosclerótica/sangre , ARN/fisiología , Ribonucleasas/fisiología , Animales , Aterosclerosis/sangre , Aterosclerosis/inducido químicamente , Aterosclerosis/tratamiento farmacológico , Dieta Alta en Grasa/efectos adversos , Líquido Extracelular/enzimología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Placa Aterosclerótica/inducido químicamente , Placa Aterosclerótica/tratamiento farmacológico , ARN/sangre , Ribonucleasas/uso terapéuticoRESUMEN
To address pivotal roles of cell surface F1/FO ATP synthase in the development of acidic microenvironment in tumor tissues, we investigated effects of shear stress stimulation on the cultured human breast cancer cells, MDA-MB-231 and MDA-MB-157, or human melanoma cells, SK-Mel-1. Shear stress stimulation (0.5-5.0 dyn/cm(2)), the levels of which are similar to those produced by the interstitial flow, induced strength-dependent corelease of ATP and H(+) from the cells, which triggered CO2 gas excretion. In contrast, the same level of shear stress stimulation did not induce significant ATP release and CO2 gas excretion from the control human mammary epithelial cells (HMEC). Marked immunocytochemical and mRNA expression of cell surface F1/FO ATP synthase, vacuolar-ATPase (V-ATPase), carbonic anhydrase type IX, and ectonucleoside triphosphate diphosphohydrolase (ENTPDase) 3 were detected in MDA-MB-231 cells, but little or no expression on the HMEC. Pretreatment with cell surface F1/FO ATP synthase inhibitors, but not cell surface V-ATPase inhibitors, caused a significant reduction of the shear stress stimulation-mediated ATP release and CO2 gas excretion from MDA-MB-231 cells. The ENTPDase activity in the shear stress-loaded MDA-MB-231 cell culture medium supernatant increased significantly in a time-dependent manner. In addition, MDA-MB-231 cells displayed strong staining for purinergic 2Y1 (P2Y1) receptors on their surfaces, and the receptors partially colocalized with ENTPDase 3. These findings suggest that cell surface F1/FO ATP synthase, but not V-ATPase, may play key roles in the development of interstitial flow-mediated acidic microenvironment in tumor tissues through the shear stress stimulation-induced ATP and H(+) corelease and CO2 gas production.
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Membrana Celular/enzimología , Líquido Extracelular/enzimología , ATPasas de Translocación de Protón/biosíntesis , Resistencia al Corte/fisiología , Microambiente Tumoral/fisiología , Técnicas de Cultivo de Célula/métodos , Línea Celular Tumoral , Membrana Celular/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Líquido Extracelular/efectos de los fármacos , Humanos , Concentración de Iones de Hidrógeno , ATPasas de Translocación de Protón/antagonistas & inhibidores , Resistencia al Corte/efectos de los fármacosRESUMEN
The bacterium Streptomyces coelicolor produces useful antibiotics from its secondary metabolites. DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in S. coelicolor through the DraR/DraK two-component system. Here, the extracellular sensory domain of DraK was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to 2.2 Å resolution and belonged to space group C2221, with unit-cell parameters a = 41.91, b = 174.50, c = 145.25 Å, α = ß = γ = 90°.
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Proteínas Bacterianas/biosíntesis , Líquido Extracelular/enzimología , Regulación Bacteriana de la Expresión Génica , Proteínas Quinasas/biosíntesis , Streptomyces coelicolor/enzimología , Proteínas Bacterianas/química , Proteínas Bacterianas/aislamiento & purificación , Cristalización , Cristalografía por Rayos X , Histidina Quinasa , Proteínas Quinasas/química , Proteínas Quinasas/aislamiento & purificación , Estructura Terciaria de ProteínaRESUMEN
Pancreatic enzymes, such as trypsin and lipase, are essential for the digestion of dietary components in the small intestine. Measurement of both enzymes in jejunal fluid and fecal specimens from dogs has not been reported and will be a prelude for further investigations. Therefore, the aim of the study was to validate radioimmunoassays (RIAs) for the measurement of canine trypsin-like immunoreactivity (cTLI) and pancreatic lipase immunoreactivity (cPLI) in jejunal fluid and fecal specimens from dogs. Jejunal fluid and fecal specimens were collected from five healthy Beagles. A commercial (125)I-RIA was used for measuring cTLI concentrations and an in-house (125)I-RIA was modified for the quantification of cPLI in jejunal fluid and fecal specimens. Both RIAs were analytically validated for canine jejunal fluid and fecal specimens by determining dilutional parallelism, spiking recovery, and intra- and inter-assay variability. For both cTLI and cPLI in jejunal fluid, observed-to-expected ratios for dilutional parallelism and spiking recovery ranged from ≥77.0% to ⩽115.3% and ≥79.0% to ≤ 120.0%, respectively, and from ≥87.2% to ≤ 118.5% and ≥74.6% to ≤ 116.1%, respectively, for fecal specimens. Intra- and inter-assay coefficients of variation (%CV) for both cTLI and cPLI in jejunal fluid were ≤ 7.6% and ≤ 10.0%, respectively, and were ≤ 10.8% and ≤ 9.0%, respectively, for fecal specimens. Both RIAs were demonstrated to be linear, accurate, precise, and reproducible for use with jejunal fluid and fecal specimens from dogs. These results are important for the investigation of pancreatic enzyme concentrations in the gastrointestinal lumen in response to changes in dietary components.
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Perros/metabolismo , Líquido Extracelular/enzimología , Heces/enzimología , Yeyuno/enzimología , Radioinmunoensayo/métodos , Animales , Femenino , Lipasa/metabolismo , Radioinmunoensayo/veterinaria , Sensibilidad y Especificidad , Tripsina/metabolismo , Tripsinógeno/metabolismoRESUMEN
Purkinje neurons are a sensitive and specialised cell type important for fine motor movement and coordination. Purkinje cell damage manifests as motor incoordination and ataxia - a prominent feature of many human disorders including spinocerebellar ataxia and Huntington's disease. A correlation between Purkinje degeneration and excess cerebellar levels of tissue-type plasminogen activator (tPA) has been observed in multiple genetically-distinct models of ataxia. Here we show that Purkinje loss in a mouse model of Huntington's disease also correlates with a 200% increase in cerebellar tPA activity. That elevated tPA levels arise in a variety of ataxia models suggests that tPA is a common mediator of Purkinje damage. To address the specific contribution of tPA to cerebellar dysfunction we studied the T4 mice line that overexpresses murine tPA in postnatal neurons through the Thy1.2 gene promoter, which directs preferential expression to Purkinje cells within the cerebellum. Here we show that T4 mice develop signs of cerebellar damage within 10 weeks of birth including atrophy of Purkinje cell soma and dendrites, astrogliosis, reduced molecular layer volume and altered gait. In contrast, T4 mice displayed no evidence of microgliosis, nor any changes in interneuron density, nor alteration in the cerebellar granular neuron layer. Thus, excess tPA levels may be sufficient to cause targeted Purkinje cell degeneration and ataxia. We propose that elevated cerebellar tPA levels exert a common pathway of Purkinje cell damage. Therapeutically lowering cerebellar tPA levels may represent a novel means of preserving Purkinje cell integrity and motor coordination across a wide range of neurodegenerative diseases.