RESUMEN
During blood stage Plasmodium falciparum infection, merozoites invade uninfected erythrocytes via a complex, multistep process involving a series of distinct receptor-ligand binding events. Understanding each element in this process increases the potential to block the parasite's life cycle via drugs or vaccines. To investigate specific receptor-ligand interactions, they were systematically blocked using a combination of genetic deletion, enzymatic receptor cleavage and inhibition of binding via antibodies, peptides and small molecules, and the resulting temporal changes in invasion and morphological effects on erythrocytes were filmed using live cell imaging. Analysis of the videos have shown receptor-ligand interactions occur in the following sequence with the following cellular morphologies; 1) an early heparin-blockable interaction which weakly deforms the erythrocyte, 2) EBA and PfRh ligands which strongly deform the erythrocyte, a process dependant on the merozoite's actin-myosin motor, 3) a PfRh5-basigin binding step which results in a pore or opening between parasite and host through which it appears small molecules and possibly invasion components can flow and 4) an AMA1-RON2 interaction that mediates tight junction formation, which acts as an anchor point for internalization. In addition to enhancing general knowledge of apicomplexan biology, this work provides a rational basis to combine sequentially acting merozoite vaccine candidates in a single multi-receptor-blocking vaccine.
Asunto(s)
Eritrocitos/parasitología , Interacciones Huésped-Parásitos , Malaria Falciparum/sangre , Malaria Falciparum/parasitología , Plasmodium falciparum/patogenicidad , Receptores de Superficie Celular/metabolismo , Animales , Antígenos de Protozoos/metabolismo , Basigina/metabolismo , Calcio/metabolismo , Proteínas Portadoras/metabolismo , Forma de la Célula , Células Cultivadas , Eritrocitos/metabolismo , Eritrocitos/patología , Interacciones Huésped-Parásitos/fisiología , Ligandos , Malaria Falciparum/metabolismo , Proteínas de la Membrana/metabolismo , Merozoítos/metabolismo , Merozoítos/patología , Plasmodium falciparum/metabolismo , Unión Proteica , Proteínas Protozoarias/metabolismo , Conejos , Transducción de SeñalRESUMEN
The liver stage is the first stage of the malaria parasite that replicates in the vertebrate host. However, little is known about the interplay between the parasite liver stage and its host cell, the hepatocyte. In this study, we identified an exported protein that has a critical role in parasite development in host hepatocytes. Expressed sequence tag analysis of Plasmodium berghei liver-stage parasites indicated that transcripts encoding a protein with an N-terminal signal peptide, designated liver-specific protein 2 (LISP2), are highly expressed in this stage. Expression of LISP2 was first observed 24 h after infection and rapidly increased during the liver-stage schizogony. Immunofluorescent staining with anti-LSP2 antibodies showed that LISP2 was carried to the parasitophorous vacuole and subsequently transported to the cytoplasm and nucleus of host hepatocytes. Gene targeting experiments demonstrated that majority of the LISP2-mutant liver-stage parasites arrested their development during formation of merozoites. These results indicate that exported LISP2 is involved in parasite-host interactions required for the development of liver-stage parasites inside hepatocytes. This study demonstrated that mid-to-late liver-stage malarial parasites have a system for exporting proteins to the host cell as intraerythrocytic stages do and presumably to use the proteins to modify the host cell and improve the environment.
Asunto(s)
Hepatocitos/metabolismo , Hepatocitos/parasitología , Merozoítos/crecimiento & desarrollo , Plasmodium berghei/crecimiento & desarrollo , Plasmodium berghei/metabolismo , Proteínas Protozoarias/metabolismo , Citoplasma/metabolismo , Etiquetas de Secuencia Expresada , Hepatocitos/citología , Interacciones Huésped-Parásitos , Humanos , Hígado/parasitología , Malaria/parasitología , Merozoítos/patología , Plasmodium berghei/genética , Plasmodium berghei/inmunología , Regiones Promotoras Genéticas , Señales de Clasificación de Proteína/genética , Transporte de Proteínas , Proteínas Protozoarias/genéticaRESUMEN
An intense and generalized sporozoan infection was detected in every population of the queen conch, Strombus gigas through the Caribbean. In this contribution we establish the relationship between occurrences of an Apicomplexa: Emeriidae-like organism and reproductive activity at San Andres archipelago, Colombia. Occurrence of the parasites was estimated counting the feeding stage Merozoites and cysts Sporozoites at 40× magnification. Nonmetric multidimensional scaling analysis (NMDS) was made to correlate the parasites stages abundance with frequency of the reproductive stages. Gametogenesis and spawning were always low coinciding with high numbers of Merozoites, a positive correlation was established between parasite abundance with reabsorption and undifferentiated stages, and negative correlation was observed between parasite abundance with maturity and spawning stages. The nonmetric multidimensional scaling (NMDS) shows that gametogenesis, maturity and spawning increase as the number of parasites decrease, factor that could be threatening reproduction of S. gigas through the Caribbean.
Asunto(s)
Apicomplexa/fisiología , Sistema Digestivo/parasitología , Gastrópodos/parasitología , Infecciones Protozoarias en Animales/parasitología , Animales , Apicomplexa/aislamiento & purificación , Sistema Digestivo/patología , Femenino , Gastrópodos/fisiología , Interacciones Huésped-Parásitos , Masculino , Merozoítos/parasitología , Merozoítos/patología , Infecciones Protozoarias en Animales/patología , Infecciones Protozoarias en Animales/fisiopatología , Reproducción/fisiología , Estaciones del AñoRESUMEN
Sequestration of parasitized erythrocytes and dysregulation of the coagulation and complement system are hallmarks of severe Plasmodium falciparum malaria. A link between these events emerged through the discovery that the parasite digestive vacuole (DV), which is released together with infective merozoites into the bloodstream, dually activates the intrinsic clotting and alternative complement pathway. Complement attack occurs exclusively on the membrane of the DVs, and the question followed whether DVs might be marked for uptake by polymorphonuclear granulocytes (PMNs). We report that DVs are indeed rapidly phagocytosed by PMNs after schizont rupture in active human serum. Uptake of malaria pigment requires an intact DV membrane and does not occur when the pigment is extracted from the organelle. Merozoites are not opsonized and escape phagocytosis in nonimmune serum. Antimalarial Abs mediate some uptake of the parasites, but to an extent that is not sufficient to markedly reduce reinvasion rates. Phagocytosis of DVs induces a vigorous respiratory burst that drives the cells into a state of functional exhaustion, blunting the production of reactive oxygen species (ROS) and microbicidal activity upon challenge with bacterial pathogens. Systemic overloading of PMNs with DVs may contribute to the enhanced susceptibility of patients with severe malaria toward invasive bacterial infections.
Asunto(s)
Neutrófilos/parasitología , Fagocitosis/fisiología , Plasmodium falciparum/patogenicidad , Vacuolas/fisiología , Animales , Recuento de Células Sanguíneas , Muerte Celular/inmunología , Eritrocitos/parasitología , Eritrocitos/patología , Humanos , Malaria Falciparum/sangre , Malaria Falciparum/inmunología , Malaria Falciparum/metabolismo , Malaria Falciparum/parasitología , Merozoítos/inmunología , Merozoítos/metabolismo , Merozoítos/patología , Merozoítos/fisiología , Modelos Biológicos , Neutrófilos/inmunología , Neutrófilos/metabolismo , Neutrófilos/fisiología , Fagocitosis/inmunología , Plasmodium falciparum/inmunología , Plasmodium falciparum/metabolismo , Plasmodium falciparum/ultraestructura , Especies Reactivas de Oxígeno/metabolismo , Especificidad por Sustrato , Factores de Tiempo , Vacuolas/metabolismo , Vacuolas/parasitologíaRESUMEN
Vector ticks possess a unique system that enables them to digest large amounts of host blood and to transmit various animal and human pathogens, suggesting the existence of evolutionally acquired proteolytic mechanisms. We report here the molecular and reverse genetic characterization of a multifunctional cysteine protease, longipain, from the babesial parasite vector tick Haemaphysalis longicornis. Longipain shares structural similarity with papain-family cysteine proteases obtained from invertebrates and vertebrates. Endogenous longipain was mainly expressed in the midgut epithelium and was specifically localized at lysosomal vacuoles and possibly released into the lumen. Its expression was up-regulated by host blood feeding. Enzymatic functional assays using in vitro and in vivo substrates revealed that longipain hydrolysis occurs over a broad range of pH and temperature. Haemoparasiticidal assays showed that longipain dose-dependently killed tick-borne Babesia parasites, and its babesiacidal effect occurred via specific adherence to the parasite membranes. Disruption of endogenous longipain by RNA interference revealed that longipain is involved in the digestion of the host blood meal. In addition, the knockdown ticks contained an increased number of parasites, suggesting that longipain exerts a killing effect against the midgut-stage Babesia parasites in ticks. Our results suggest that longipain is essential for tick survival, and may have a role in controlling the transmission of tick-transmittable Babesia parasites.